Difference between revisions of "Team:HK GTC"

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                    <h1>PRACTICAL</h1>
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                    <p>PETase & Related Analogous Chimeras Transfused in Computer and AI Learning</p>
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                <h1>Project Abstract</h1>
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<p>Plastic pollution has been a global issue since the last century. In this study, we provide solutions to alleviate the plastic pollution problem from multiple perspectives. We develop a dual enzyme system as chimeras between PETase and MHETase to degrade polyethylene terephthalate (PET) into its constituent monomers. The performance of PETase and MHETase cocktail mixtures is also compared for the extent of hydrolysis of amorphous PET film, and the mixture exhibits improved depolymerization activity compared with the single enzyme. A survey of 60 items, aimed to investigate knowledge, values and actions of secondary students towards plastic pollution, was designed and conducted in 4 secondary schools. The findings suggest the need for environmental education to engage students to take part in preserving the natural environment. Drone and AI technology was applied to train and develop a deep learning PET bottle detection model, which maps plastic pollution on beaches.</p>
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<h1> Welcome to iGEM 2021! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li> <a href="https://2021.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2021.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2021.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<p> You must upload any pictures and files to the iGEM 2021 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2021.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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EDIT PAGE
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2021.igem.org/Calendar">iGEM 2021 calendar</a> </li>
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                        <h1>Hover over the Bottle!</h1>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                        <h1>Team</h1>
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<li> <a href="https://2019.igem.org/Team:Vilnius-Lithuania"> 2019 Vilnius Lithuania</a> </li>
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                        <p>We are team HK_GTC - A secondary school team from Hong Kong. Click to explore more about the
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<li> <a href="https://2019.igem.org/Team:NUS_Singapore">2019 NUS Singapore</a> </li>
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us.</p>
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<li> <a href="https://2019.igem.org/Team:Lambert_GA">2019 Lambert GA</a> </li>
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<li> <a href="https://2020.igem.org/Team:Waterloo"> 2020 Waterloo</a></li>
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<li> <a href="https://2020.igem.org/Team:TUDelft">2020 TUDelft</a></li>
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<li> <a href="https://2020.igem.org/Team:XMU-China"> 2020 XMU China </a></li>
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                        <h1>Project</h1>
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<li> <a href="https://2020.igem.org/Team:TAS_Taipei"> 2020 TAS Taipei </a></li>
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                        <p>Our research team develops different dual enzyme systems of PETase and MHETase to increase the rate of PET depolymerization. We constructed chimeras between PETase and MHETase, with the C-terminus of PETase linked to the N-terminus of MHETase using a 12 amino acid link. The degradation rates of PET film using PETase-MHETase chimera, as well as PETase and MHETase cocktail mixtures, are compared. We have shown that the two enzymes act synergistically, increasing the efficiency of the digestion of PET into its final monomers, TPA and EG, and that the mixture exhibits improved depolymerization activity compared with the single enzyme.
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                        <h1>Safety</h1>
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                        <h1>Parts</h1>
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                        <h1>Human Practice</h1>
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                        <p>Human practice team is responsible for raising the awareness of the plastic pollution problem, and our project. We have interviewed local professionals from relevant fields and integrated their advice into our project to strive for improvement. Aside from that, we have also done some educational work. Click to explore.</p>
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                        <h1>Deep Learning</h1>
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                        <p>Responding to the plastic pollution problem, the team created a plastic bottle detecting deep learning model to map PET bottles on beaches. The data allows government, councils, NGO to have an overview of the current situation and to estimate how effective their proposal is to reduce the impact of plastic wastes. Our ultimate goal is to help to reduce the amount of plastic pollution in the ocean. </p>
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                            <a href="https://2021.igem.org/Team:HK_GTC/Team"><span><div class="link"></div></span></a>
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                        <img id="bot-project-im" class="showable" src="https://static.igem.org/mediawiki/2021/a/ad/T--HK_GTC--home_bot_purple_plastic.png">
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                        <a href="https://2021.igem.org/Team:HK_GTC/Description"><span><div class="link"></div></span></a>
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                        <img id="bot-project-im" class="showable"
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                            src="https://static.igem.org/mediawiki/2021/d/da/T--HK_GTC--top-right-bottle.png">
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                        <a href="https://2021.igem.org/Team:HK_GTC/Parts"><span><div class="link"></div></span></a>
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                        <img id="bot-part-im" class="showable"
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                            src="https://static.igem.org/mediawiki/2021/7/72/T--HK_GTC--home_bot_blue_plastic.png">
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                    <div id="bot-safety" class="bot-button">
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                        <a href="https://2021.igem.org/Team:HK_GTC/Safety"><span><div class="link"></div></span></a>
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                        <img id="bot-safe-im" class="showable"
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                            src="https://static.igem.org/mediawiki/2021/2/2c/T--HK_GTC--right-bottle.png">
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                        <a href="https://2021.igem.org/Team:HK_GTC/Deep_learning"><span><div class="link"></div></span></a>
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                        <img id="bot-dl-im" class="showable" src="https://static.igem.org/mediawiki/2021/4/4d/T--HK_GTC--bot-left-bottle.png">
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                        <a href="https://2021.igem.org/Team:HK_GTC/Human_Practices"><span><div class="link"></div></span></a>
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                        <a href="#welcome"><span><div class="link"></div></span></a>
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        </section>
  
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        <footer>
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                <h1>GT COLLEGE iGEM2021</h1>
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                <img width="30%" src="https://static.igem.org/mediawiki/2021/7/78/T--HK_GTC--logo.png" alt="">
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                <h1>Follow Us!</h1>
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                <h1>Contact:</h1>
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                <p>igemteam.gt@gmail.com</p>
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Latest revision as of 23:11, 28 November 2021

HK_GTC 2021 Homepage

PRACTICAL

PETase & Related Analogous Chimeras Transfused in Computer and AI Learning

Project Abstract

Plastic pollution has been a global issue since the last century. In this study, we provide solutions to alleviate the plastic pollution problem from multiple perspectives. We develop a dual enzyme system as chimeras between PETase and MHETase to degrade polyethylene terephthalate (PET) into its constituent monomers. The performance of PETase and MHETase cocktail mixtures is also compared for the extent of hydrolysis of amorphous PET film, and the mixture exhibits improved depolymerization activity compared with the single enzyme. A survey of 60 items, aimed to investigate knowledge, values and actions of secondary students towards plastic pollution, was designed and conducted in 4 secondary schools. The findings suggest the need for environmental education to engage students to take part in preserving the natural environment. Drone and AI technology was applied to train and develop a deep learning PET bottle detection model, which maps plastic pollution on beaches.

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com