Difference between revisions of "Team:TecCEM/Results Zetasizer"

 
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e&&e.upscaleMethod&&"string"==typeof e.upscaleMethod&&n.upscaleMethods[e.upscaleMethod.toUpperCase()]||n.upscaleMethods.AUTO},e.exports.roundToFixed=g},function(e,t,i){"use strict";var r={isWEBP:i(0).noWEBP,isObjectFitBrowser:!0};e.exports.getFeature=function(e){return r[e]},e.exports.setFeature=function(e,t){r[e]=t}},function(e,t,i){"use strict";function r(e){return(r="function"==typeof Symbol&&"symbol"==typeof Symbol.iterator?function(e){return typeof e}:function(e){return e&&"function"==typeof Symbol&&e.constructor===Symbol&&e!==Symbol.prototype?"symbol":typeof e})(e)}e.exports.includes=function(e,t){return e.indexOf?e.indexOf(t)>-1:!(!e||"object"!==r(e))&&Object.keys(e).some((function(i){return e[i]===t}))},e.exports.last=function(e){return e[e.length-1]},e.exports.template=function(e){return function(t){var i=e;for(var r in t)t.hasOwnProperty(r)&&(i=i.replace(new RegExp("\\${"+r+"}","g"),t[r]));return i}}},function(e,t,i){"use strict";function r(e,t){var i=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter((function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable}))),i.push.apply(i,r)}return i}function n(e,t,i){return t in e?Object.defineProperty(e,t,{value:i,enumerable:!0,configurable:!0,writable:!0}):e[t]=i,e}var o=i(1),a=o.isSEOBot,s=o.getFileType,c=o.getFileName,u=o.getFileExtension,l=o.getDevicePixelRatio,h=o.getUpscaleString,d=o.isImageTransformApplicable,f=i(7),p=f.isMobile,g=f.isWEBPBrowserSupport,m=i(10),b=i(11),v=i(0);e.exports.getTransform=function(e,t,i,r){var n=a(r),o=s(t.id),f=c(t.id,t.name),p=u(t.id),v=!n&&g(o),T=n?1:l(i),_={fileName:f,fileExtension:p,fileType:o,isWEBPSupport:v,fittingType:e,preferredExtension:v?"webp":p,src:{id:t.id,width:t.width,height:t.height,isCropped:!1},focalPoint:{x:t.focalPoint&&t.focalPoint.x,y:t.focalPoint&&t.focalPoint.y},parts:[],devicePixelRatio:T,quality:0,upscaleMethod:h(r),progressive:!0,watermark:"",unsharpMask:{},filters:{}};return d(t.id)&&(m.setTransformParts(_,t,i),b.setTransformOptions(_,r)),_},e.exports.getTarget=function(e,t,i){var o=function(e){for(var t=1;t<arguments.length;t++){var i=null!=arguments[t]?arguments[t]:{};t%2?r(Object(i),!0).forEach((function(t){n(e,t,i[t])})):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(i)):r(Object(i)).forEach((function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(i,t))}))}return e}({},i),a=p();switch(e){case v.fittingTypes.LEGACY_BG_FIT_AND_TILE:case v.fittingTypes.LEGACY_BG_FIT_AND_TILE_HORIZONTAL:case v.fittingTypes.LEGACY_BG_FIT_AND_TILE_VERTICAL:case v.fittingTypes.LEGACY_BG_NORMAL:var s=a?v.MOBILE_MAX_BG_SITE_LEGACY_WIDTH:v.DSKTP_MAX_BG_SITE_LEGACY_WIDTH,c=a?v.MOBILE_MAX_BG_SITE_LEGACY_HEIGHT:v.DSKTP_MAX_BG_SITE_LEGACY_HEIGHT;o.width=Math.min(s,t.width),o.height=Math.min(c,Math.round(o.width/(t.width/t.height))),o.pixelAspectRatio=1}return o}},function(e,t,i){"use strict";var r=i(2),n=i(0);function o(e){var t=r.getFeature("isWEBP"),i=new window.Image;i.onload=function(){t[e]=i.width>0&&i.height>0,r.setFeature("isWEBP",t)},i.onerror=function(){t[e]=!1,r.setFeature("isWEBP",t)},i.src="data:image/webp;base64,"+{lossy:"UklGRiIAAABXRUJQVlA4IBYAAAAwAQCdASoBAAEADsD+JaQAA3AAAAAA",lossless:"UklGRhoAAABXRUJQVlA4TA0AAAAvAAAAEAcQERGIiP4HAA==",alpha:"UklGRkoAAABXRUJQVlA4WAoAAAAQAAAAAAAAAAAAQUxQSAwAAAARBxAR/Q9ERP8DAABWUDggGAAAABQBAJ0BKgEAAQAAAP4AAA3AAP7mtQAAAA==",animation:"UklGRlIAAABXRUJQVlA4WAoAAAASAAAAAAAAAAAAQU5JTQYAAAD/////AABBTk1GJgAAAAAAAAAAAAAAAAAAAGQAAABWUDhMDQAAAC8AAAAQBxAREYiI/gcA"}[e]}e.exports.checkSupportByUserAgent=function(e,t){var i=t.browser,o=t.os;if(e){var a,s=parseFloat(i.version),c=parseFloat(o.version),u=new RegExp(/AppleWebKit\/([\d.]+)/),l=null===u.exec(e)?null:parseFloat(u.exec(e)[1]),h=function(e,t,i,r,n){var o=!e.phone&&!e.tablet&&i.chrome&&r>=23,a=e.android&&(e.phone||e.tablet)&&i.webkit&&i.chrome&&r>=25,s=e.android&&n<535&&(e.phone||e.tablet)&&t>=4.2&&i.webkit&&!i.safari,c=i.edge&&r>=18,u=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(o||a||s||c||u)}(o,c,i,s,l);r.setFeature("isWEBP",((a={})[n.webp.LOSSY]=function(e,t,i,r,n){var o=!e.phone&&!e.tablet&&i.chrome&&r>=17,a=e.android&&(e.phone||e.tablet)&&i.webkit&&i.chrome&&r>=25,s=e.android&&n<535&&(e.phone||e.tablet)&&t>=4&&i.webkit,c=i.edge&&r>=18,u=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(o||a||s||c||u)}(o,c,i,s,l),a[n.webp.LOSSLESS]=h,a[n.webp.ALPHA]=h,a[n.webp.ANIMATION]=function(e,t,i,r){var n=!e.ios&&i.chrome&&r>=32,o=i.edge&&r>=18,a=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(n||o||a)}(o,0,i,s),a))}},e.exports.checkSupportByFeatureDetection=function(){o(n.webp.LOSSY),o(n.webp.LOSSLESS),o(n.webp.ALPHA),o(n.webp.ANIMATION)},e.exports.isWEBPBrowserSupport=function(e){var t=r.getFeature("isWEBP"),i=e===n.fileType.JPG&&t[n.webp.LOSSY],o=e===n.fileType.PNG&&t[n.webp.LOSSLESS],a=e===n.fileType.PNG&&t[n.webp.ALPHA];return i||o&&a}},function(e,t,i){"use strict";var r=i(0).noWEBP,n=i(9),o=i(5),a=i(2);e.exports.populateGlobalFeatureSupport=function(e){var t;void 0===e&&(e=""),"undefined"!=typeof window&&"undefined"!=typeof navigator?((t=n(navigator.userAgent)).browser.safari||t.os.iphone||t.os.ipad?a.setFeature("isWEBP",r):(o.checkSupportByUserAgent(navigator.userAgent,t),o.checkSupportByFeatureDetection()),a.setFeature("isObjectFitBrowser","objectFit"in window.document.documentElement.style),a.setFeature("isMobile",t.os.phone)):(t=n(e),o.checkSupportByUserAgent(e,t),a.setFeature("isMobile",t.os.phone))}},function(e,t,i){"use strict";var r=i(5),n=i(2);e.exports.isWEBPBrowserSupport=r.isWEBPBrowserSupport,e.exports.isObjectFitBrowserSupport=function(){return n.getFeature("isObjectFitBrowser")},e.exports.isMobile=function(){return n.getFeature("isMobile")}},function(e,t,i){"use strict";var r=i(0),n=i(1).isImageTransformApplicable,o=i(12),a=i(4);e.exports.getURI=function(e,t,i,s,c){var u=r.emptyData.uri;return n(t.id)?(c=c||a.getTransform(e,t,i,s,c),u=o.getImageURI(c)):u=t.id,u}},function(e,t,i){e.exports=function(){"use strict";return function(e){var t={},i={};if(!e)return{browser:i,os:t};var r=e.match(/Web[kK]it[\/]{0,1}([\d.]+)/),n=e.match(/(Android);?[\s\/]+([\d.]+)?/),o=!!e.match(/\(Macintosh\; Intel /),a=e.match(/(iPad).*OS\s([\d_]+)/),s=e.match(/(iPod)(.*OS\s([\d_]+))?/),c=!a&&e.match(/(iPhone\sOS)\s([\d_]+)/),u=e.match(/(webOS|hpwOS)[\s\/]([\d.]+)/),l=e.match(/Windows Phone ([\d.]+)/),h=u&&e.match(/TouchPad/),d=e.match(/Kindle\/([\d.]+)/),f=e.match(/Silk\/([\d._]+)/),p=e.match(/(BlackBerry).*Version\/([\d.]+)/),g=e.match(/(BB10).*Version\/([\d.]+)/),m=e.match(/(RIM\sTablet\sOS)\s([\d.]+)/),b=e.match(/PlayBook/),v=e.match(/Chrome\/([\d.]+)/)||e.match(/CriOS\/([\d.]+)/),T=e.match(/Firefox\/([\d.]+)/),_=e.match(/MSIE\s([\d.]+)/)||e.match(/Trident\/[\d](?=[^\?]+).*rv:([0-9.].)/),y=!v&&e.match(/(iPhone|iPod|iPad).*AppleWebKit(?!.*Safari)/),I=y||e.match(/Version\/([\d.]+)([^S](Safari)|[^M]*(Mobile)[^S]*(Safari))/),E=e.match(/Edge\/(\d{2,}\.[\d\w]+)$/),A=e.match(/Opera Mini/);return i.webkit=r&&!E,i.webkit&&(i.version=r[1]),n&&(t.android=!0,t.version=n[2]),c&&!s&&(t.ios=t.iphone=!0,t.version=c[2].replace(/_/g,".")),a&&(t.ios=t.ipad=!0,t.version=a[2].replace(/_/g,".")),s&&(t.ios=t.ipod=!0,t.version=s[3]?s[3].replace(/_/g,"."):null),l&&(t.wp=!0,t.version=l[1]),u&&(t.webos=!0,t.version=u[2]),h&&(t.touchpad=!0),p&&(t.blackberry=!0,t.version=p[2]),g&&(t.bb10=!0,t.version=g[2]),m&&(t.rimtabletos=!0,t.version=m[2]),b&&(i.playbook=!0),d&&(t.kindle=!0,t.version=d[1]),f&&(i.silk=!0,i.version=f[1]),!f&&t.android&&e.match(/Kindle Fire/)&&(i.silk=!0),v&&!E&&(i.chrome=!0,i.version=v[1]),T&&!E&&(i.firefox=!0,i.version=T[1]),_&&(i.ie=!0,i.version=_[1]),I&&(o||t.ios)&&(i.safari=!0,o&&(i.version=I[1])),y&&(i.webview=!0),E&&(i.edge=!0,i.version=E[1]),A&&(i.operaMini=!0),t.tablet=!!(a||b||n&&!e.match(/Mobile/)||T&&e.match(/Tablet/)||(_||E)&&!e.match(/Phone/)&&e.match(/Touch/)),t.phone=!(t.tablet||t.ipod||!(n||c||u||p||g||v&&e.match(/Android/)||v&&e.match(/CriOS\/([\d.]+)/)||T&&e.match(/Mobile/)||_&&e.match(/Touch/))),t.mac=o,t.googleBot=!!e.match(/Googlebot\/2.1/),{browser:i,os:t}}}()},function(e,t,i){"use strict";var r=i(0),n=i(1),o=n.getAlignment,a=n.getScaleFactor,s=n.getOverlappingRect,c=n.getAlignedRect,u=n.getTransformData,l=n.getFocalPoint;function h(e,t){var i=u(e.src.width,e.src.height,t,e.devicePixelRatio,r.transformTypes.FIT,e.upscaleMethod);return{transformType:r.transformTypes.FILL,width:Math.round(i.width),height:Math.round(i.height),alignment:r.alignTypesMap.center,upscale:i.scaleFactor>1,forceUSM:i.forceUSM,scaleFactor:i.scaleFactor,cssUpscaleNeeded:i.cssUpscaleNeeded,upscaleMethodValue:i.upscaleMethodValue}}function d(e){return{transformType:r.transformTypes.CROP,x:Math.round(e.x),y:Math.round(e.y),width:Math.round(e.width),height:Math.round(e.height),upscale:!1,forceUSM:!1,scaleFactor:1,cssUpscaleNeeded:!1}}e.exports.setTransformParts=function(e,t,i){var n;switch(t.crop&&(n=s(t,t.crop))&&(e.src.width=n.width,e.src.height=n.height,e.src.cropped=!0,e.parts.push(d(n))),e.fittingType){case r.fittingTypes.SCALE_TO_FIT:case r.fittingTypes.LEGACY_FIT_WIDTH:case r.fittingTypes.LEGACY_FIT_HEIGHT:case r.fittingTypes.LEGACY_FULL:case r.fittingTypes.FIT_AND_TILE:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE_HORIZONTAL:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE_VERTICAL:case r.fittingTypes.LEGACY_BG_NORMAL:e.parts.push(h(e,i));break;case r.fittingTypes.SCALE_TO_FILL:e.parts.push(function(e,t){var i=u(e.src.width,e.src.height,t,e.devicePixelRatio,r.transformTypes.FILL,e.upscaleMethod),n=l(e.focalPoint);return{transformType:n?r.transformTypes.FILL_FOCAL:r.transformTypes.FILL,width:Math.round(i.width),height:Math.round(i.height),alignment:o(t),focalPointX:n&&n.x,focalPointY:n&&n.y,upscale:i.scaleFactor>1,forceUSM:i.forceUSM,scaleFactor:i.scaleFactor,cssUpscaleNeeded:i.cssUpscaleNeeded,upscaleMethodValue:i.upscaleMethodValue}}(e,i));break;case r.fittingTypes.STRETCH:e.parts.push(function(e,t){var i=a(e.src.width,e.src.height,t.width,t.height,r.transformTypes.FILL),n=Object.assign({},t);return n.width=e.src.width*i,n.height=e.src.height*i,h(e,n)}(e,i));break;case r.fittingTypes.TILE_HORIZONTAL:case r.fittingTypes.TILE_VERTICAL:case r.fittingTypes.TILE:case r.fittingTypes.LEGACY_ORIGINAL_SIZE:case r.fittingTypes.ORIGINAL_SIZE:n=c(e.src,e.focalPoint,i,i.alignment),e.src.isCropped?(Object.assign(e.parts[0],n),e.src.width=n.width,e.src.height=n.height):e.parts.push(d(n));break;case r.fittingTypes.LEGACY_STRIP_TILE_HORIZONTAL:case r.fittingTypes.LEGACY_STRIP_TILE_VERTICAL:case r.fittingTypes.LEGACY_STRIP_TILE:case r.fittingTypes.LEGACY_STRIP_ORIGINAL_SIZE:e.parts.push(function(e){return{transformType:r.transformTypes.LEGACY_CROP,width:Math.round(e.width),height:Math.round(e.height),alignment:o(e),upscale:!1,forceUSM:!1,scaleFactor:1,cssUpscaleNeeded:!1}}(i));break;case r.fittingTypes.LEGACY_STRIP_SCALE_TO_FIT:case r.fittingTypes.LEGACY_STRIP_FIT_AND_TILE:e.parts.push(function(e){return{transformType:r.transformTypes.FIT,width:Math.round(e.width),height:Math.round(e.height),upscale:!1,forceUSM:!0,scaleFactor:1,cssUpscaleNeeded:!1}}(i));break;case r.fittingTypes.LEGACY_STRIP_SCALE_TO_FILL:e.parts.push(function(e){return{transformType:r.transformTypes.LEGACY_FILL,width:Math.round(e.width),height:Math.round(e.height),alignment:o(e),upscale:!1,forceUSM:!0,scaleFactor:1,cssUpscaleNeeded:!1}}(i))}}},function(e,t,i){"use strict";var r=i(3),n=i(0),o=i(1),a=o.getPreferredImageQuality,s=o.roundToFixed;function c(e,t,i){return!isNaN(e)&&"number"==typeof e&&0!==e&&e>=t&&e<=i}e.exports.setTransformOptions=function(e,t){t=t||{},e.quality=function(e,t){var i=e.fileType===n.fileType.PNG&&e.isWEBPSupport;if(e.fileType===n.fileType.JPG||i){var o=r.last(e.parts),s=a(o.width,o.height),c=t.quality&&t.quality>=5&&t.quality<=90?t.quality:s;return c=i?c+5:c,parseInt(c,10)}return 0}(e,t),e.progressive=function(e){return!1!==e.progressive}(t),e.watermark=function(e){return e.watermark}(t),e.unsharpMask=function(e,t){var i;return function(e){e=e||{};var t=!isNaN(e.radius)&&"number"==typeof e.radius&&e.radius>=.1&&e.radius<=500,i=!isNaN(e.amount)&&"number"==typeof e.amount&&e.amount>=0&&e.amount<=10,r=!isNaN(e.threshold)&&"number"==typeof e.threshold&&e.threshold>=0&&e.threshold<=255;return t&&i&&r}(t.unsharpMask)?i={radius:t.unsharpMask.radius,amount:t.unsharpMask.amount,threshold:t.unsharpMask.threshold}:function(e){return e=e||{},!isNaN(e.radius)&&"number"==typeof e.radius&&0===e.radius&&!isNaN(e.amount)&&"number"==typeof e.amount&&0===e.amount&&!isNaN(e.threshold)&&"number"==typeof e.threshold&&0===e.threshold}(t.unsharpMask)||function(e){var t=r.last(e.parts);return!(t.scaleFactor>=1)||t.forceUSM}(e)&&(i=n.defaultUSM),i&&(i.radius=s(i.radius,2),i.amount=s(i.amount,2),i.threshold=s(i.threshold,2)),i}(e,t),e.filters=function(e){var t=e.filters||{},i={};return c(t[n.imageFilters.CONTRAST],-100,100)&&(i[n.imageFilters.CONTRAST]=t[n.imageFilters.CONTRAST]),c(t[n.imageFilters.BRIGHTNESS],-100,100)&&(i[n.imageFilters.BRIGHTNESS]=t[n.imageFilters.BRIGHTNESS]),c(t[n.imageFilters.SATURATION],-100,100)&&(i[n.imageFilters.SATURATION]=t[n.imageFilters.SATURATION]),c(t[n.imageFilters.HUE],-180,180)&&(i[n.imageFilters.HUE]=t[n.imageFilters.HUE]),c(t[n.imageFilters.BLUR],0,100)&&(i[n.imageFilters.BLUR]=t[n.imageFilters.BLUR]),i}(t)}},function(e,t,i){"use strict";var r,n=i(3),o=i(0),a=o.imageFilters,s=o.transformTypes,c=o.API_VERSION,u=n.template("fit/w_${width},h_${height}"),l=n.template("fill/w_${width},h_${height},al_${alignment}"),h=n.template("fill/w_${width},h_${height},fp_${focalPointX}_${focalPointY}"),d=n.template("crop/x_${x},y_${y},w_${width},h_${height}"),f=n.template("crop/w_${width},h_${height},al_${alignment}"),p=n.template("fill/w_${width},h_${height},al_${alignment}"),g=n.template(",lg_${upscaleMethodValue}"),m=n.template(",q_${quality}"),b=n.template(",usm_${radius}_${amount}_${threshold}"),v=n.template(",bl"),T=n.template(",wm_${watermark}"),_=((r={})[a.CONTRAST]=n.template(",con_${contrast}"),r[a.BRIGHTNESS]=n.template(",br_${brightness}"),r[a.SATURATION]=n.template(",sat_${saturation}"),r[a.HUE]=n.template(",hue_${hue}"),r[a.BLUR]=n.template(",blur_${blur}"),r);e.exports.getImageURI=function(e){var t=[];e.parts.forEach((function(e){switch(e.transformType){case s.CROP:t.push(d(e));break;case s.LEGACY_CROP:t.push(f(e));break;case s.LEGACY_FILL:var i=p(e);e.upscale&&(i+=g(e)),t.push(i);break;case s.FIT:var r=u(e);e.upscale&&(r+=g(e)),t.push(r);break;case s.FILL:var n=l(e);e.upscale&&(n+=g(e)),t.push(n);break;case s.FILL_FOCAL:var o=h(e);e.upscale&&(o+=g(e)),t.push(o)}}));var i=t.join("/");return e.quality&&(i+=m(e)),e.unsharpMask&&(i+=b(e.unsharpMask)),e.progressive||(i+=v(e)),e.watermark&&(i+=T(e)),e.filters&&(i+=Object.keys(e.filters).map((function(t){return _[t](e.filters)})).join("")),e.src.id+"/"+c+"/"+i+"/"+e.fileName+"."+e.preferredExtension}},function(e,t,i){"use strict";var r=i(14),n=i(0),o=/^media\//i,a="undefined"!=typeof window?window.devicePixelRatio:1,s=function(e,t){var i=(void 0===t?{}:t).baseHostURL;return i?""+i+e:function(e){return o.test(e)?"https://static.wixstatic.com/"+e:"https://static.wixstatic.com/media/"+e}(e)};e.exports.populateGlobalFeatureSupport=r.populateGlobalFeatureSupport,e.exports.getScaleToFitImageURL=function(e,t,i,o,c,u){var l=r.getData(n.fittingTypes.SCALE_TO_FIT,{id:e,width:t,height:i,name:u&&u.name},{width:o,height:c,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},u);return s(l.uri,u)},e.exports.getScaleToFillImageURL=function(e,t,i,o,c,u){var l=r.getData(n.fittingTypes.SCALE_TO_FILL,{id:e,width:t,height:i,name:u&&u.name,focalPoint:{x:u&&u.focalPoint&&u.focalPoint.x,y:u&&u.focalPoint&&u.focalPoint.y}},{width:o,height:c,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},u);return s(l.uri,u)},e.exports.getCropImageURL=function(e,t,i,o,c,u,l,h,d,f){var p=r.getData(n.fittingTypes.SCALE_TO_FILL,{id:e,width:t,height:i,name:f&&f.name,crop:{x:o,y:c,width:u,height:l}},{width:h,height:d,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},f);return s(p.uri,f)}},function(e,t,i){"use strict";var r=i(0),n=i(1).isValidRequest,o=i(6).populateGlobalFeatureSupport,a=i(4),s=i(8).getURI;e.exports.populateGlobalFeatureSupport=o,e.exports.getData=function(e,t,i,o){var c=r.emptyData.uri;if(n(e,t,i)){var u=a.getTarget(e,t,i),l=a.getTransform(e,t,u,o);c=s(e,t,u,o,l)}return{uri:c}},e.exports.fittingTypes=r.fittingTypes,e.exports.alignTypes=r.alignTypes,e.exports.htmlTag=r.htmlTag,e.exports.upscaleMethods=r.upscaleMethods},function(e,t,i){"use strict";var r=i(0),n=i(6).populateGlobalFeatureSupport,o=i(16),a=o.getData,s=o.getPlaceholder,c=i(13),u=c.getScaleToFitImageURL,l=c.getScaleToFillImageURL,h=c.getCropImageURL;n(),e.exports.populateGlobalFeatureSupport=n,e.exports.getData=a,e.exports.getPlaceholder=s,e.exports.fittingTypes=r.fittingTypes,e.exports.alignTypes=r.alignTypes,e.exports.htmlTag=r.htmlTag,e.exports.upscaleMethods=r.upscaleMethods,e.exports.sdk={getScaleToFitImageURL:u,getScaleToFillImageURL:l,getCropImageURL:h}},function(e,t,i){"use strict";function r(e,t){var i=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter((function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable}))),i.push.apply(i,r)}return i}function n(e){for(var t=1;t<arguments.length;t++){var i=null!=arguments[t]?arguments[t]:{};t%2?r(Object(i),!0).forEach((function(t){o(e,t,i[t])})):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(i)):r(Object(i)).forEach((function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(i,t))}))}return e}function o(e,t,i){return t in e?Object.defineProperty(e,t,{value:i,enumerable:!0,configurable:!0,writable:!0}):e[t]=i,e}var a=i(0),s=i(1).isValidRequest,c=i(17),u=i(4),l=i(8).getURI,h=i(22),d=h.getScaledDimensions,f=h.getBlurValue,p=h.getCSSOverrides,g=h.validateTargetDimensions,m=h.getIsFakeTile,b=h.getConvertedFitting;function v(e,t,i,r){var n={};if(s(e,t,i)){var o=u.getTarget(e,t,i),h=u.getTransform(e,t,o,r);n.uri=l(e,t,o,r,h),Object.assign(n,c.getAttributes(h,o))}else n=a.emptyData;return n}e.exports.getData=v,e.exports.getPlaceholder=function(e,t,i,r){if(void 0===r&&(r={}),!s(e,t,i))return a.emptyData;var o=n(n({},i),g(t,i)),c=i.alignment,u=i.htmlTag,l=r.isSEOBot,h=m(e,t,o),T=d(e,t,o,l),_=f(o.width,e,l),y=b(e,h),I=p(e,t,i,c),E=v(y,t,n(n({},T),{},{alignment:c,htmlTag:u}),{filters:{blur:_}}).uri,A=v(e,t,n(n({},o),{},{alignment:c,htmlTag:u})),w=A.attr,O=void 0===w?{}:w,S=A.css;return S.img=S.img||{},S.container=S.container||{},Object.assign(S.img,I.img),Object.assign(S.container,I.container),{uri:E,css:S,attr:O}}},function(e,t,i){"use strict";var r=i(0).htmlTag,n=i(7).isObjectFitBrowserSupport,o=i(18),a=i(19),s=i(20),c=i(21);e.exports.getAttributes=function(e,t){return(t.htmlTag===r.BG?o:t.htmlTag===r.SVG?s:n()?a:c).get(e,t)}},function(e,t,i){"use strict";var r=i(0),n=r.alignTypes,o=r.fittingTypes;e.exports.get=function(e,t){var i={css:{container:{}}},r=i.css;switch(e.fittingType){case o.ORIGINAL_SIZE:case o.LEGACY_ORIGINAL_SIZE:case o.LEGACY_STRIP_ORIGINAL_SIZE:r.container.backgroundSize="auto",r.container.backgroundRepeat="no-repeat";break;case o.SCALE_TO_FIT:case o.LEGACY_STRIP_SCALE_TO_FIT:r.container.backgroundSize="contain",r.container.backgroundRepeat="no-repeat";break;case o.STRETCH:r.container.backgroundSize="100% 100%",r.container.backgroundRepeat="no-repeat";break;case o.SCALE_TO_FILL:case o.LEGACY_STRIP_SCALE_TO_FILL:r.container.backgroundSize="cover",r.container.backgroundRepeat="no-repeat";break;case o.TILE_HORIZONTAL:case o.LEGACY_STRIP_TILE_HORIZONTAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-x";break;case o.TILE_VERTICAL:case o.LEGACY_STRIP_TILE_VERTICAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-y";break;case o.TILE:case o.LEGACY_STRIP_TILE:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat";break;case o.LEGACY_STRIP_FIT_AND_TILE:r.container.backgroundSize="contain",r.container.backgroundRepeat="repeat";break;case o.FIT_AND_TILE:case o.LEGACY_BG_FIT_AND_TILE:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat";break;case o.LEGACY_BG_FIT_AND_TILE_HORIZONTAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-x";break;case o.LEGACY_BG_FIT_AND_TILE_VERTICAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-y";break;case o.LEGACY_BG_NORMAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="no-repeat"}switch(t.alignment){case n.CENTER:r.container.backgroundPosition="center center";break;case n.LEFT:r.container.backgroundPosition="left center";break;case n.RIGHT:r.container.backgroundPosition="right center";break;case n.TOP:r.container.backgroundPosition="center top";break;case n.BOTTOM:r.container.backgroundPosition="center bottom";break;case n.TOP_RIGHT:r.container.backgroundPosition="right top";break;case n.TOP_LEFT:r.container.backgroundPosition="left top";break;case n.BOTTOM_RIGHT:r.container.backgroundPosition="right bottom";break;case n.BOTTOM_LEFT:r.container.backgroundPosition="left bottom"}return i}},function(e,t,i){"use strict";var r,n=i(0),o=n.alignTypes,a=n.fittingTypes,s=((r={})[o.CENTER]="center",r[o.TOP]="top",r[o.TOP_LEFT]="top left",r[o.TOP_RIGHT]="top right",r[o.BOTTOM]="bottom",r[o.BOTTOM_LEFT]="bottom left",r[o.BOTTOM_RIGHT]="bottom right",r[o.LEFT]="left",r[o.RIGHT]="right",r),c={position:"absolute",top:"auto",right:"auto",bottom:"auto",left:"auto"};e.exports={get:function(e,t){var i={css:{container:{},img:{}}},r=i.css,n=e.fittingType,u=t.alignment;switch(r.container.position="relative",n){case a.ORIGINAL_SIZE:case a.LEGACY_ORIGINAL_SIZE:e.parts&&e.parts.length?(r.img.width=e.parts[0].width,r.img.height=e.parts[0].height):(r.img.width=e.src.width,r.img.height=e.src.height);break;case a.SCALE_TO_FIT:case a.LEGACY_FIT_WIDTH:case a.LEGACY_FIT_HEIGHT:case a.LEGACY_FULL:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="contain",r.img.objectPosition=s[u]||"unset";break;case a.LEGACY_BG_NORMAL:r.img.width="100%",r.img.height="100%",r.img.objectFit="none",r.img.objectPosition=s[u]||"unset";break;case a.STRETCH:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="fill";break;case a.SCALE_TO_FILL:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="cover"}if("number"==typeof r.img.width&&(r.img.width!==t.width||r.img.height!==t.height)){var l=Math.round((t.height-r.img.height)/2),h=Math.round((t.width-r.img.width)/2);Object.assign(r.img,c,function(e,t,i){var r;return(r={})[o.TOP_LEFT]={top:0,left:0},r[o.TOP_RIGHT]={top:0,right:0},r[o.TOP]={top:0,left:t},r[o.BOTTOM_LEFT]={top:0,left:0},r[o.BOTTOM_RIGHT]={bottom:0,right:0},r[o.BOTTOM]={bottom:0,left:t},r[o.RIGHT]={top:e,right:0},r[o.LEFT]={top:e,left:0},r[o.CENTER]={width:i.width,height:i.height,objectFit:"none"},r}(l,h,t)[u])}return i}}},function(e,t,i){"use strict";var r=i(0),n=r.fittingTypes,o=r.alignTypes,a=r.transformTypes,s=i(1),c=s.getDimension,u=s.isImageTransformApplicable;e.exports={get:function(e,t){var i,r={css:{container:{}},attr:{container:{},img:{}}},s=r.css,l=r.attr,h=e.fittingType,d=t.alignment,f=e.src,p=f.width,g=f.height;switch(s.container.position="relative",h){case n.ORIGINAL_SIZE:case n.LEGACY_ORIGINAL_SIZE:case n.TILE:e.parts&&e.parts.length?(l.img.width=e.parts[0].width,l.img.height=e.parts[0].height):(l.img.width=p,l.img.height=g),l.img.preserveAspectRatio="xMidYMid slice";break;case n.SCALE_TO_FIT:case n.LEGACY_FIT_WIDTH:case n.LEGACY_FIT_HEIGHT:case n.LEGACY_FULL:l.img.width="100%",l.img.height="100%",l.img.transform="",l.img.preserveAspectRatio="";break;case n.STRETCH:l.img.width=t.width,l.img.height=t.height,l.img.x=0,l.img.y=0,l.img.transform="",l.img.preserveAspectRatio="none";break;case n.SCALE_TO_FILL:u(e.src.id)?(l.img.width=t.width,l.img.height=t.height):(i=c(p,g,t.width,t.height,a.FILL),l.img.width=i.width,l.img.height=i.height),l.img.x=0,l.img.y=0,l.img.transform="",l.img.preserveAspectRatio="xMidYMid slice"}if("number"==typeof l.img.width&&(l.img.width!==t.width||l.img.height!==t.height)){var m,b,v=0,T=0;h===n.TILE?(m=t.width%l.img.width,b=t.height%l.img.height):(m=t.width-l.img.width,b=t.height-l.img.height);var _=Math.round(m/2),y=Math.round(b/2);switch(d){case o.TOP_LEFT:v=0,T=0;break;case o.TOP:v=_,T=0;break;case o.TOP_RIGHT:v=m,T=0;break;case o.LEFT:v=0,T=y;break;case o.CENTER:v=_,T=y;break;case o.RIGHT:v=m,T=y;break;case o.BOTTOM_LEFT:v=0,T=b;break;case o.BOTTOM:v=_,T=b;break;case o.BOTTOM_RIGHT:v=m,T=b}l.img.x=v,l.img.y=T}return l.container.width=t.width,l.container.height=t.height,l.container.viewBox=[0,0,t.width,t.height].join(" "),r}}},function(e,t,i){"use strict";var r=i(0),n=r.fittingTypes,o=r.alignTypes,a=r.transformTypes,s=i(1),c=s.getDimension,u=s.isImageTypeSupported,l=s.isExternalUrl;e.exports={get:function(e,t){var i,r,s,h={css:{container:{},img:{}}},d=h.css,f=e.fittingType,p=t.alignment;switch(e.parts&&e.parts.length?(i=e.parts[0].width,r=e.parts[0].height):(i=e.src.width,r=e.src.height),d.img.display="block",d.container.position="relative",d.img.position="absolute",d.img.top="auto",d.img.right="auto",d.img.bottom="auto",d.img.left="auto",f){case n.ORIGINAL_SIZE:case n.LEGACY_ORIGINAL_SIZE:d.img.width=i,d.img.height=r;break;case n.SCALE_TO_FIT:case n.LEGACY_FIT_WIDTH:case n.LEGACY_FIT_HEIGHT:case n.LEGACY_FULL:s=c(i,r,t.width,t.height,a.FIT),Object.assign(d.img,s);break;case n.STRETCH:d.img.width=t.width,d.img.height=t.height;break;case n.SCALE_TO_FILL:var g=e.src.id;u(g)||l(g)?(d.img.width=t.width,d.img.height=t.height):(s=c(i,r,t.width,t.height,a.FILL),Object.assign(d.img,s),d.container.overflow="hidden")}if(d.img.width!==t.width||d.img.height!==t.height){var m=Math.round((t.height-d.img.height)/2),b=Math.round((t.width-d.img.width)/2);switch(p){default:case o.CENTER:d.img.top=m,d.img.left=b;break;case o.LEFT:d.img.left=0,d.img.top=m;break;case o.RIGHT:d.img.right=0,d.img.top=m;break;case o.TOP:d.img.left=b,d.img.top=0;break;case o.BOTTOM:d.img.left=b,d.img.bottom=0;break;case o.TOP_RIGHT:d.img.right=0,d.img.top=0;break;case o.TOP_LEFT:d.img.left=0,d.img.top=0;break;case o.BOTTOM_RIGHT:d.img.right=0,d.img.bottom=0;break;case o.BOTTOM_LEFT:d.img.left=0,d.img.bottom=0}}return h}}},function(e,t,i){"use strict";var r,n=i(0),o=n.alignTypes,a=n.fittingTypes,s=((r={})[o.CENTER]="50% 50%",r[o.TOP_LEFT]="0% 0%",r[o.TOP_RIGHT]="100% 0%",r[o.TOP]="50% 0%",r[o.BOTTOM_LEFT]="0% 100%",r[o.BOTTOM_RIGHT]="100% 100%",r[o.BOTTOM]="50% 100%",r[o.RIGHT]="100% 50%",r[o.LEFT]="0% 50%",r),c=Object.entries(s).reduce((function(e,t){var i=t[0];return e[t[1]]=i,e}),{}),u=[a.TILE,a.TILE_HORIZONTAL,a.TILE_VERTICAL,a.LEGACY_BG_FIT_AND_TILE,a.LEGACY_BG_FIT_AND_TILE_HORIZONTAL,a.LEGACY_BG_FIT_AND_TILE_VERTICAL],l=[a.LEGACY_ORIGINAL_SIZE,a.ORIGINAL_SIZE,a.LEGACY_BG_NORMAL];function h(e,t,i){var r=i.width,n=i.height;return e===a.TILE&&t.width>r&&t.height>n}function d(e,t,i){var r=e.width,n=e.height,o=t.width,a=t.height,s=i.x,c=i.y;if(!o||!a)return s+"% "+c+"%";var u=Math.max(o/r,a/n),l=r*u,h=n*u,d=Math.max(0,Math.min(l-o,l*(s/100)-o/2)),f=Math.max(0,Math.min(h-a,h*(c/100)-a/2));return(d&&Math.floor(d/(l-o)*100))+"% "+(f&&Math.floor(f/(h-a)*100))+"%"}e.exports.validateTargetDimensions=function(e,t){var i=t.width,r=t.height;if(!i||!r){var n=i||Math.min(980,e.width),o=n/e.width;return{width:n,height:r||e.height*o}}return{width:i,height:r}},e.exports.getScaledDimensions=function(e,t,i,r){var n=i.width,o=i.height;if(void 0===r&&(r=!1),r)return{width:n,height:o};var a=!l.includes(e),s=h(e,t,{width:n,height:o}),c=!s&&u.includes(e),d=c?t.width:n,f=c?t.height:o,p=a?function(e){return e>900?.25:e>500?.3:e>200?.4:1}(d):1;return{width:s?1920:d*p,height:f*p}},e.exports.getConvertedFitting=function(e,t){var i=u.includes(e)&&!t;return e===a.SCALE_TO_FILL||i?a.SCALE_TO_FIT:e},e.exports.getIsFakeTile=h,e.exports.getBlurValue=function(e,t,i){return i?0:u.includes(t)?1:e>200?2:3},e.exports.getCSSOverrides=function(e,t,i,r){void 0===r&&(r="center");var n,o,l={img:{},container:{}};if(e===a.SCALE_TO_FILL){var h=t.focalPoint&&(o=(n=t.focalPoint).x+"% "+n.y+"%",c[o]||""),f=h||r;t.focalPoint&&!h?l.img={objectPosition:d(t,i,t.focalPoint)}:l.img={objectPosition:s[f]}}else[a.LEGACY_ORIGINAL_SIZE,a.ORIGINAL_SIZE].includes(e)?l.img={objectFit:"none",top:"auto",left:"auto",right:"auto",bottom:"auto"}:u.includes(e)&&(l.container={backgroundSize:t.width+"px "+t.height+"px"});return l}}])},50343:function(){!function(){if(void 0!==window.Reflect&&void 0!==window.customElements&&!window.customElements.hasOwnProperty("polyfillWrapFlushCallback")){var e=HTMLElement;window.HTMLElement=function(){return Reflect.construct(e,[],this.constructor)},HTMLElement.prototype=e.prototype,HTMLElement.prototype.constructor=HTMLElement,Object.setPrototypeOf(HTMLElement,e)}}()},58699:function(e){"use strict";var t=function(e){return e*Math.PI/180},i=function(e,t){return{width:e,height:t}},r=function(e,t,i){return{width:e,height:Math.max(t,i)}};e.exports={BackgroundParallax:{hasParallax:!0,getMediaDimensions:r},BackgroundParallaxZoom:{hasParallax:!0,getMediaDimensions:r},BackgroundReveal:{hasParallax:!0,getMediaDimensions:r},BgCloseUp:{getMediaDimensions:i},BgExpand:{getMediaDimensions:i},BgFabeBack:{getMediaDimensions:i},BgFadeIn:{getMediaDimensions:i},BgFadeOut:{getMediaDimensions:i},BgFake3D:{hasParallax:!0,getMediaDimensions:r},BgPanLeft:{getMediaDimensions:function(e,t){return{width:1.2*e,height:t}}},BgPanRight:{getMediaDimensions:function(e,t){return{width:1.2*e,height:t}}},BgParallax:{hasParallax:!0,getMediaDimensions:r},BgPullBack:{getMediaDimensions:i},BgReveal:{hasParallax:!0,getMediaDimensions:r},BgRotate:{getMediaDimensions:function(e,i){return function(e,i,r){var n=t(r),o=Math.hypot(e,i)/2,a=Math.acos(e/2/o),s=e*Math.abs(Math.cos(n))+i*Math.abs(Math.sin(n)),c=e*Math.abs(Math.sin(n))+i*Math.abs(Math.cos(n));return{width:Math.ceil(n<a?s:2*o),height:Math.ceil(n<t(90)-a?c:2*o)}}(e,i,22)}},BgShrink:{getMediaDimensions:i},BgSkew:{getMediaDimensions:function(e,i){return function(e,i,r){var n=t(r);return{width:e,height:e*Math.tan(n)+i}}(e,i,20)}},BgUnwind:{getMediaDimensions:i},BgZoomIn:{hasParallax:!0,getMediaDimensions:r},BgZoomOut:{getMediaDimensions:function(e,t){return{width:1.15*e,height:1.15*t}}}}},17948:function(e,t,i){"use strict";var r=i(31191),n=i(96114),o=i.n(n),a=i(76702),s=i(21405),c=i(58699),u=i.n(c),l=i(46436),h=i.n(l),d=function(e,t,i){var r=(Array.isArray(t)?t:t.split(".")).reduce((function(e,t){return e&&void 0!==e[t]?e[t]:null}),e);return null!==r?r:i},f=function(e,t){return(Array.isArray(t)?t:[t]).reduce((function(t,i){var r,n=d(e,i);return void 0!==n?Object.assign(t,((r={})[i]=n,r)):t}),{})};function p(){for(var 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window.Image;i.onload=function(){t[e]=i.width>0&&i.height>0,r.setFeature("isWEBP",t)},i.onerror=function(){t[e]=!1,r.setFeature("isWEBP",t)},i.src="data:image/webp;base64,"+{lossy:"UklGRiIAAABXRUJQVlA4IBYAAAAwAQCdASoBAAEADsD+JaQAA3AAAAAA",lossless:"UklGRhoAAABXRUJQVlA4TA0AAAAvAAAAEAcQERGIiP4HAA==",alpha:"UklGRkoAAABXRUJQVlA4WAoAAAAQAAAAAAAAAAAAQUxQSAwAAAARBxAR/Q9ERP8DAABWUDggGAAAABQBAJ0BKgEAAQAAAP4AAA3AAP7mtQAAAA==",animation:"UklGRlIAAABXRUJQVlA4WAoAAAASAAAAAAAAAAAAQU5JTQYAAAD/////AABBTk1GJgAAAAAAAAAAAAAAAAAAAGQAAABWUDhMDQAAAC8AAAAQBxAREYiI/gcA"}[e]}e.exports.checkSupportByUserAgent=function(e,t){var i=t.browser,o=t.os;if(e){var a,s=parseFloat(i.version),c=parseFloat(o.version),u=new RegExp(/AppleWebKit\/([\d.]+)/),l=null===u.exec(e)?null:parseFloat(u.exec(e)[1]),h=function(e,t,i,r,n){var o=!e.phone&&!e.tablet&&i.chrome&&r>=23,a=e.android&&(e.phone||e.tablet)&&i.webkit&&i.chrome&&r>=25,s=e.android&&n<535&&(e.phone||e.tablet)&&t>=4.2&&i.webkit&&!i.safari,c=i.edge&&r>=18,u=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(o||a||s||c||u)}(o,c,i,s,l);r.setFeature("isWEBP",((a={})[n.webp.LOSSY]=function(e,t,i,r,n){var o=!e.phone&&!e.tablet&&i.chrome&&r>=17,a=e.android&&(e.phone||e.tablet)&&i.webkit&&i.chrome&&r>=25,s=e.android&&n<535&&(e.phone||e.tablet)&&t>=4&&i.webkit,c=i.edge&&r>=18,u=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(o||a||s||c||u)}(o,c,i,s,l),a[n.webp.LOSSLESS]=h,a[n.webp.ALPHA]=h,a[n.webp.ANIMATION]=function(e,t,i,r){var n=!e.ios&&i.chrome&&r>=32,o=i.edge&&r>=18,a=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!!(n||o||a)}(o,0,i,s),a))}},e.exports.checkSupportByFeatureDetection=function(){o(n.webp.LOSSY),o(n.webp.LOSSLESS),o(n.webp.ALPHA),o(n.webp.ANIMATION)},e.exports.isWEBPBrowserSupport=function(e){var t=r.getFeature("isWEBP"),i=e===n.fileType.JPG&&t[n.webp.LOSSY],o=e===n.fileType.PNG&&t[n.webp.LOSSLESS],a=e===n.fileType.PNG&&t[n.webp.ALPHA];return i||o&&a}},function(e,t,i){"use strict";var r=i(0).noWEBP,n=i(9),o=i(5),a=i(2);e.exports.populateGlobalFeatureSupport=function(e){var t;void 0===e&&(e=""),"undefined"!=typeof window&&"undefined"!=typeof navigator?((t=n(navigator.userAgent)).browser.safari||t.os.iphone||t.os.ipad?a.setFeature("isWEBP",r):(o.checkSupportByUserAgent(navigator.userAgent,t),o.checkSupportByFeatureDetection()),a.setFeature("isObjectFitBrowser","objectFit"in window.document.documentElement.style),a.setFeature("isMobile",t.os.phone)):(t=n(e),o.checkSupportByUserAgent(e,t),a.setFeature("isMobile",t.os.phone))}},function(e,t,i){"use strict";var r=i(5),n=i(2);e.exports.isWEBPBrowserSupport=r.isWEBPBrowserSupport,e.exports.isObjectFitBrowserSupport=function(){return n.getFeature("isObjectFitBrowser")},e.exports.isMobile=function(){return n.getFeature("isMobile")}},function(e,t,i){"use strict";var r=i(0),n=i(1).isImageTransformApplicable,o=i(12),a=i(4);e.exports.getURI=function(e,t,i,s,c){var u=r.emptyData.uri;return n(t.id)?(c=c||a.getTransform(e,t,i,s,c),u=o.getImageURI(c)):u=t.id,u}},function(e,t,i){e.exports=function(){"use strict";return function(e){var t={},i={};if(!e)return{browser:i,os:t};var r=e.match(/Web[kK]it[\/]{0,1}([\d.]+)/),n=e.match(/(Android);?[\s\/]+([\d.]+)?/),o=!!e.match(/\(Macintosh\; Intel /),a=e.match(/(iPad).*OS\s([\d_]+)/),s=e.match(/(iPod)(.*OS\s([\d_]+))?/),c=!a&&e.match(/(iPhone\sOS)\s([\d_]+)/),u=e.match(/(webOS|hpwOS)[\s\/]([\d.]+)/),l=e.match(/Windows Phone ([\d.]+)/),h=u&&e.match(/TouchPad/),d=e.match(/Kindle\/([\d.]+)/),f=e.match(/Silk\/([\d._]+)/),p=e.match(/(BlackBerry).*Version\/([\d.]+)/),g=e.match(/(BB10).*Version\/([\d.]+)/),m=e.match(/(RIM\sTablet\sOS)\s([\d.]+)/),b=e.match(/PlayBook/),v=e.match(/Chrome\/([\d.]+)/)||e.match(/CriOS\/([\d.]+)/),T=e.match(/Firefox\/([\d.]+)/),_=e.match(/MSIE\s([\d.]+)/)||e.match(/Trident\/[\d](?=[^\?]+).*rv:([0-9.].)/),y=!v&&e.match(/(iPhone|iPod|iPad).*AppleWebKit(?!.*Safari)/),I=y||e.match(/Version\/([\d.]+)([^S](Safari)|[^M]*(Mobile)[^S]*(Safari))/),E=e.match(/Edge\/(\d{2,}\.[\d\w]+)$/),A=e.match(/Opera Mini/);return i.webkit=r&&!E,i.webkit&&(i.version=r[1]),n&&(t.android=!0,t.version=n[2]),c&&!s&&(t.ios=t.iphone=!0,t.version=c[2].replace(/_/g,".")),a&&(t.ios=t.ipad=!0,t.version=a[2].replace(/_/g,".")),s&&(t.ios=t.ipod=!0,t.version=s[3]?s[3].replace(/_/g,"."):null),l&&(t.wp=!0,t.version=l[1]),u&&(t.webos=!0,t.version=u[2]),h&&(t.touchpad=!0),p&&(t.blackberry=!0,t.version=p[2]),g&&(t.bb10=!0,t.version=g[2]),m&&(t.rimtabletos=!0,t.version=m[2]),b&&(i.playbook=!0),d&&(t.kindle=!0,t.version=d[1]),f&&(i.silk=!0,i.version=f[1]),!f&&t.android&&e.match(/Kindle Fire/)&&(i.silk=!0),v&&!E&&(i.chrome=!0,i.version=v[1]),T&&!E&&(i.firefox=!0,i.version=T[1]),_&&(i.ie=!0,i.version=_[1]),I&&(o||t.ios)&&(i.safari=!0,o&&(i.version=I[1])),y&&(i.webview=!0),E&&(i.edge=!0,i.version=E[1]),A&&(i.operaMini=!0),t.tablet=!!(a||b||n&&!e.match(/Mobile/)||T&&e.match(/Tablet/)||(_||E)&&!e.match(/Phone/)&&e.match(/Touch/)),t.phone=!(t.tablet||t.ipod||!(n||c||u||p||g||v&&e.match(/Android/)||v&&e.match(/CriOS\/([\d.]+)/)||T&&e.match(/Mobile/)||_&&e.match(/Touch/))),t.mac=o,t.googleBot=!!e.match(/Googlebot\/2.1/),{browser:i,os:t}}}()},function(e,t,i){"use strict";var r=i(0),n=i(1),o=n.getAlignment,a=n.getScaleFactor,s=n.getOverlappingRect,c=n.getAlignedRect,u=n.getTransformData,l=n.getFocalPoint;function h(e,t){var i=u(e.src.width,e.src.height,t,e.devicePixelRatio,r.transformTypes.FIT,e.upscaleMethod);return{transformType:r.transformTypes.FILL,width:Math.round(i.width),height:Math.round(i.height),alignment:r.alignTypesMap.center,upscale:i.scaleFactor>1,forceUSM:i.forceUSM,scaleFactor:i.scaleFactor,cssUpscaleNeeded:i.cssUpscaleNeeded,upscaleMethodValue:i.upscaleMethodValue}}function d(e){return{transformType:r.transformTypes.CROP,x:Math.round(e.x),y:Math.round(e.y),width:Math.round(e.width),height:Math.round(e.height),upscale:!1,forceUSM:!1,scaleFactor:1,cssUpscaleNeeded:!1}}e.exports.setTransformParts=function(e,t,i){var n;switch(t.crop&&(n=s(t,t.crop))&&(e.src.width=n.width,e.src.height=n.height,e.src.cropped=!0,e.parts.push(d(n))),e.fittingType){case r.fittingTypes.SCALE_TO_FIT:case r.fittingTypes.LEGACY_FIT_WIDTH:case r.fittingTypes.LEGACY_FIT_HEIGHT:case r.fittingTypes.LEGACY_FULL:case r.fittingTypes.FIT_AND_TILE:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE_HORIZONTAL:case r.fittingTypes.LEGACY_BG_FIT_AND_TILE_VERTICAL:case r.fittingTypes.LEGACY_BG_NORMAL:e.parts.push(h(e,i));break;case r.fittingTypes.SCALE_TO_FILL:e.parts.push(function(e,t){var i=u(e.src.width,e.src.height,t,e.devicePixelRatio,r.transformTypes.FILL,e.upscaleMethod),n=l(e.focalPoint);return{transformType:n?r.transformTypes.FILL_FOCAL:r.transformTypes.FILL,width:Math.round(i.width),height:Math.round(i.height),alignment:o(t),focalPointX:n&&n.x,focalPointY:n&&n.y,upscale:i.scaleFactor>1,forceUSM:i.forceUSM,scaleFactor:i.scaleFactor,cssUpscaleNeeded:i.cssUpscaleNeeded,upscaleMethodValue:i.upscaleMethodValue}}(e,i));break;case r.fittingTypes.STRETCH:e.parts.push(function(e,t){var i=a(e.src.width,e.src.height,t.width,t.height,r.transformTypes.FILL),n=Object.assign({},t);return n.width=e.src.width*i,n.height=e.src.height*i,h(e,n)}(e,i));break;case r.fittingTypes.TILE_HORIZONTAL:case r.fittingTypes.TILE_VERTICAL:case r.fittingTypes.TILE:case r.fittingTypes.LEGACY_ORIGINAL_SIZE:case r.fittingTypes.ORIGINAL_SIZE:n=c(e.src,e.focalPoint,i,i.alignment),e.src.isCropped?(Object.assign(e.parts[0],n),e.src.width=n.width,e.src.height=n.height):e.parts.push(d(n));break;case r.fittingTypes.LEGACY_STRIP_TILE_HORIZONTAL:case r.fittingTypes.LEGACY_STRIP_TILE_VERTICAL:case r.fittingTypes.LEGACY_STRIP_TILE:case r.fittingTypes.LEGACY_STRIP_ORIGINAL_SIZE:e.parts.push(function(e){return{transformType:r.transformTypes.LEGACY_CROP,width:Math.round(e.width),height:Math.round(e.height),alignment:o(e),upscale:!1,forceUSM:!1,scaleFactor:1,cssUpscaleNeeded:!1}}(i));break;case r.fittingTypes.LEGACY_STRIP_SCALE_TO_FIT:case r.fittingTypes.LEGACY_STRIP_FIT_AND_TILE:e.parts.push(function(e){return{transformType:r.transformTypes.FIT,width:Math.round(e.width),height:Math.round(e.height),upscale:!1,forceUSM:!0,scaleFactor:1,cssUpscaleNeeded:!1}}(i));break;case r.fittingTypes.LEGACY_STRIP_SCALE_TO_FILL:e.parts.push(function(e){return{transformType:r.transformTypes.LEGACY_FILL,width:Math.round(e.width),height:Math.round(e.height),alignment:o(e),upscale:!1,forceUSM:!0,scaleFactor:1,cssUpscaleNeeded:!1}}(i))}}},function(e,t,i){"use strict";var r=i(3),n=i(0),o=i(1),a=o.getPreferredImageQuality,s=o.roundToFixed;function c(e,t,i){return!isNaN(e)&&"number"==typeof e&&0!==e&&e>=t&&e<=i}e.exports.setTransformOptions=function(e,t){t=t||{},e.quality=function(e,t){var i=e.fileType===n.fileType.PNG&&e.isWEBPSupport;if(e.fileType===n.fileType.JPG||i){var o=r.last(e.parts),s=a(o.width,o.height),c=t.quality&&t.quality>=5&&t.quality<=90?t.quality:s;return c=i?c+5:c,parseInt(c,10)}return 0}(e,t),e.progressive=function(e){return!1!==e.progressive}(t),e.watermark=function(e){return e.watermark}(t),e.unsharpMask=function(e,t){var i;return function(e){e=e||{};var t=!isNaN(e.radius)&&"number"==typeof e.radius&&e.radius>=.1&&e.radius<=500,i=!isNaN(e.amount)&&"number"==typeof e.amount&&e.amount>=0&&e.amount<=10,r=!isNaN(e.threshold)&&"number"==typeof e.threshold&&e.threshold>=0&&e.threshold<=255;return t&&i&&r}(t.unsharpMask)?i={radius:t.unsharpMask.radius,amount:t.unsharpMask.amount,threshold:t.unsharpMask.threshold}:function(e){return e=e||{},!isNaN(e.radius)&&"number"==typeof e.radius&&0===e.radius&&!isNaN(e.amount)&&"number"==typeof e.amount&&0===e.amount&&!isNaN(e.threshold)&&"number"==typeof e.threshold&&0===e.threshold}(t.unsharpMask)||function(e){var t=r.last(e.parts);return!(t.scaleFactor>=1)||t.forceUSM}(e)&&(i=n.defaultUSM),i&&(i.radius=s(i.radius,2),i.amount=s(i.amount,2),i.threshold=s(i.threshold,2)),i}(e,t),e.filters=function(e){var t=e.filters||{},i={};return c(t[n.imageFilters.CONTRAST],-100,100)&&(i[n.imageFilters.CONTRAST]=t[n.imageFilters.CONTRAST]),c(t[n.imageFilters.BRIGHTNESS],-100,100)&&(i[n.imageFilters.BRIGHTNESS]=t[n.imageFilters.BRIGHTNESS]),c(t[n.imageFilters.SATURATION],-100,100)&&(i[n.imageFilters.SATURATION]=t[n.imageFilters.SATURATION]),c(t[n.imageFilters.HUE],-180,180)&&(i[n.imageFilters.HUE]=t[n.imageFilters.HUE]),c(t[n.imageFilters.BLUR],0,100)&&(i[n.imageFilters.BLUR]=t[n.imageFilters.BLUR]),i}(t)}},function(e,t,i){"use strict";var r,n=i(3),o=i(0),a=o.imageFilters,s=o.transformTypes,c=o.API_VERSION,u=n.template("fit/w_${width},h_${height}"),l=n.template("fill/w_${width},h_${height},al_${alignment}"),h=n.template("fill/w_${width},h_${height},fp_${focalPointX}_${focalPointY}"),d=n.template("crop/x_${x},y_${y},w_${width},h_${height}"),f=n.template("crop/w_${width},h_${height},al_${alignment}"),p=n.template("fill/w_${width},h_${height},al_${alignment}"),g=n.template(",lg_${upscaleMethodValue}"),m=n.template(",q_${quality}"),b=n.template(",usm_${radius}_${amount}_${threshold}"),v=n.template(",bl"),T=n.template(",wm_${watermark}"),_=((r={})[a.CONTRAST]=n.template(",con_${contrast}"),r[a.BRIGHTNESS]=n.template(",br_${brightness}"),r[a.SATURATION]=n.template(",sat_${saturation}"),r[a.HUE]=n.template(",hue_${hue}"),r[a.BLUR]=n.template(",blur_${blur}"),r);e.exports.getImageURI=function(e){var t=[];e.parts.forEach((function(e){switch(e.transformType){case s.CROP:t.push(d(e));break;case s.LEGACY_CROP:t.push(f(e));break;case s.LEGACY_FILL:var i=p(e);e.upscale&&(i+=g(e)),t.push(i);break;case s.FIT:var r=u(e);e.upscale&&(r+=g(e)),t.push(r);break;case s.FILL:var n=l(e);e.upscale&&(n+=g(e)),t.push(n);break;case s.FILL_FOCAL:var o=h(e);e.upscale&&(o+=g(e)),t.push(o)}}));var i=t.join("/");return e.quality&&(i+=m(e)),e.unsharpMask&&(i+=b(e.unsharpMask)),e.progressive||(i+=v(e)),e.watermark&&(i+=T(e)),e.filters&&(i+=Object.keys(e.filters).map((function(t){return _[t](e.filters)})).join("")),e.src.id+"/"+c+"/"+i+"/"+e.fileName+"."+e.preferredExtension}},function(e,t,i){"use strict";var r=i(14),n=i(0),o=/^media\//i,a="undefined"!=typeof window?window.devicePixelRatio:1,s=function(e,t){var i=(void 0===t?{}:t).baseHostURL;return i?""+i+e:function(e){return o.test(e)?"https://static.wixstatic.com/"+e:"https://static.wixstatic.com/media/"+e}(e)};e.exports.populateGlobalFeatureSupport=r.populateGlobalFeatureSupport,e.exports.getScaleToFitImageURL=function(e,t,i,o,c,u){var l=r.getData(n.fittingTypes.SCALE_TO_FIT,{id:e,width:t,height:i,name:u&&u.name},{width:o,height:c,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},u);return s(l.uri,u)},e.exports.getScaleToFillImageURL=function(e,t,i,o,c,u){var l=r.getData(n.fittingTypes.SCALE_TO_FILL,{id:e,width:t,height:i,name:u&&u.name,focalPoint:{x:u&&u.focalPoint&&u.focalPoint.x,y:u&&u.focalPoint&&u.focalPoint.y}},{width:o,height:c,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},u);return s(l.uri,u)},e.exports.getCropImageURL=function(e,t,i,o,c,u,l,h,d,f){var p=r.getData(n.fittingTypes.SCALE_TO_FILL,{id:e,width:t,height:i,name:f&&f.name,crop:{x:o,y:c,width:u,height:l}},{width:h,height:d,htmlTag:r.htmlTag.IMG,alignment:r.alignTypes.CENTER,pixelAspectRatio:a},f);return s(p.uri,f)}},function(e,t,i){"use strict";var r=i(0),n=i(1).isValidRequest,o=i(6).populateGlobalFeatureSupport,a=i(4),s=i(8).getURI;e.exports.populateGlobalFeatureSupport=o,e.exports.getData=function(e,t,i,o){var c=r.emptyData.uri;if(n(e,t,i)){var u=a.getTarget(e,t,i),l=a.getTransform(e,t,u,o);c=s(e,t,u,o,l)}return{uri:c}},e.exports.fittingTypes=r.fittingTypes,e.exports.alignTypes=r.alignTypes,e.exports.htmlTag=r.htmlTag,e.exports.upscaleMethods=r.upscaleMethods},function(e,t,i){"use strict";var r=i(0),n=i(6).populateGlobalFeatureSupport,o=i(16),a=o.getData,s=o.getPlaceholder,c=i(13),u=c.getScaleToFitImageURL,l=c.getScaleToFillImageURL,h=c.getCropImageURL;n(),e.exports.populateGlobalFeatureSupport=n,e.exports.getData=a,e.exports.getPlaceholder=s,e.exports.fittingTypes=r.fittingTypes,e.exports.alignTypes=r.alignTypes,e.exports.htmlTag=r.htmlTag,e.exports.upscaleMethods=r.upscaleMethods,e.exports.sdk={getScaleToFitImageURL:u,getScaleToFillImageURL:l,getCropImageURL:h}},function(e,t,i){"use strict";function r(e,t){var i=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter((function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable}))),i.push.apply(i,r)}return i}function n(e){for(var t=1;t<arguments.length;t++){var i=null!=arguments[t]?arguments[t]:{};t%2?r(Object(i),!0).forEach((function(t){o(e,t,i[t])})):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(i)):r(Object(i)).forEach((function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(i,t))}))}return e}function o(e,t,i){return t in e?Object.defineProperty(e,t,{value:i,enumerable:!0,configurable:!0,writable:!0}):e[t]=i,e}var a=i(0),s=i(1).isValidRequest,c=i(17),u=i(4),l=i(8).getURI,h=i(22),d=h.getScaledDimensions,f=h.getBlurValue,p=h.getCSSOverrides,g=h.validateTargetDimensions,m=h.getIsFakeTile,b=h.getConvertedFitting;function v(e,t,i,r){var n={};if(s(e,t,i)){var o=u.getTarget(e,t,i),h=u.getTransform(e,t,o,r);n.uri=l(e,t,o,r,h),Object.assign(n,c.getAttributes(h,o))}else n=a.emptyData;return n}e.exports.getData=v,e.exports.getPlaceholder=function(e,t,i,r){if(void 0===r&&(r={}),!s(e,t,i))return a.emptyData;var o=n(n({},i),g(t,i)),c=i.alignment,u=i.htmlTag,l=r.isSEOBot,h=m(e,t,o),T=d(e,t,o,l),_=f(o.width,e,l),y=b(e,h),I=p(e,t,i,c),E=v(y,t,n(n({},T),{},{alignment:c,htmlTag:u}),{filters:{blur:_}}).uri,A=v(e,t,n(n({},o),{},{alignment:c,htmlTag:u})),w=A.attr,O=void 0===w?{}:w,S=A.css;return S.img=S.img||{},S.container=S.container||{},Object.assign(S.img,I.img),Object.assign(S.container,I.container),{uri:E,css:S,attr:O}}},function(e,t,i){"use strict";var r=i(0).htmlTag,n=i(7).isObjectFitBrowserSupport,o=i(18),a=i(19),s=i(20),c=i(21);e.exports.getAttributes=function(e,t){return(t.htmlTag===r.BG?o:t.htmlTag===r.SVG?s:n()?a:c).get(e,t)}},function(e,t,i){"use strict";var r=i(0),n=r.alignTypes,o=r.fittingTypes;e.exports.get=function(e,t){var i={css:{container:{}}},r=i.css;switch(e.fittingType){case o.ORIGINAL_SIZE:case o.LEGACY_ORIGINAL_SIZE:case o.LEGACY_STRIP_ORIGINAL_SIZE:r.container.backgroundSize="auto",r.container.backgroundRepeat="no-repeat";break;case o.SCALE_TO_FIT:case o.LEGACY_STRIP_SCALE_TO_FIT:r.container.backgroundSize="contain",r.container.backgroundRepeat="no-repeat";break;case o.STRETCH:r.container.backgroundSize="100% 100%",r.container.backgroundRepeat="no-repeat";break;case o.SCALE_TO_FILL:case o.LEGACY_STRIP_SCALE_TO_FILL:r.container.backgroundSize="cover",r.container.backgroundRepeat="no-repeat";break;case o.TILE_HORIZONTAL:case o.LEGACY_STRIP_TILE_HORIZONTAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-x";break;case o.TILE_VERTICAL:case o.LEGACY_STRIP_TILE_VERTICAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-y";break;case o.TILE:case o.LEGACY_STRIP_TILE:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat";break;case o.LEGACY_STRIP_FIT_AND_TILE:r.container.backgroundSize="contain",r.container.backgroundRepeat="repeat";break;case o.FIT_AND_TILE:case o.LEGACY_BG_FIT_AND_TILE:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat";break;case o.LEGACY_BG_FIT_AND_TILE_HORIZONTAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-x";break;case o.LEGACY_BG_FIT_AND_TILE_VERTICAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="repeat-y";break;case o.LEGACY_BG_NORMAL:r.container.backgroundSize="auto",r.container.backgroundRepeat="no-repeat"}switch(t.alignment){case n.CENTER:r.container.backgroundPosition="center center";break;case n.LEFT:r.container.backgroundPosition="left center";break;case n.RIGHT:r.container.backgroundPosition="right center";break;case n.TOP:r.container.backgroundPosition="center top";break;case n.BOTTOM:r.container.backgroundPosition="center bottom";break;case n.TOP_RIGHT:r.container.backgroundPosition="right top";break;case n.TOP_LEFT:r.container.backgroundPosition="left top";break;case n.BOTTOM_RIGHT:r.container.backgroundPosition="right bottom";break;case n.BOTTOM_LEFT:r.container.backgroundPosition="left bottom"}return i}},function(e,t,i){"use strict";var r,n=i(0),o=n.alignTypes,a=n.fittingTypes,s=((r={})[o.CENTER]="center",r[o.TOP]="top",r[o.TOP_LEFT]="top left",r[o.TOP_RIGHT]="top right",r[o.BOTTOM]="bottom",r[o.BOTTOM_LEFT]="bottom left",r[o.BOTTOM_RIGHT]="bottom right",r[o.LEFT]="left",r[o.RIGHT]="right",r),c={position:"absolute",top:"auto",right:"auto",bottom:"auto",left:"auto"};e.exports={get:function(e,t){var i={css:{container:{},img:{}}},r=i.css,n=e.fittingType,u=t.alignment;switch(r.container.position="relative",n){case a.ORIGINAL_SIZE:case a.LEGACY_ORIGINAL_SIZE:e.parts&&e.parts.length?(r.img.width=e.parts[0].width,r.img.height=e.parts[0].height):(r.img.width=e.src.width,r.img.height=e.src.height);break;case a.SCALE_TO_FIT:case a.LEGACY_FIT_WIDTH:case a.LEGACY_FIT_HEIGHT:case a.LEGACY_FULL:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="contain",r.img.objectPosition=s[u]||"unset";break;case a.LEGACY_BG_NORMAL:r.img.width="100%",r.img.height="100%",r.img.objectFit="none",r.img.objectPosition=s[u]||"unset";break;case a.STRETCH:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="fill";break;case a.SCALE_TO_FILL:r.img.width=t.width,r.img.height=t.height,r.img.objectFit="cover"}if("number"==typeof r.img.width&&(r.img.width!==t.width||r.img.height!==t.height)){var l=Math.round((t.height-r.img.height)/2),h=Math.round((t.width-r.img.width)/2);Object.assign(r.img,c,function(e,t,i){var r;return(r={})[o.TOP_LEFT]={top:0,left:0},r[o.TOP_RIGHT]={top:0,right:0},r[o.TOP]={top:0,left:t},r[o.BOTTOM_LEFT]={top:0,left:0},r[o.BOTTOM_RIGHT]={bottom:0,right:0},r[o.BOTTOM]={bottom:0,left:t},r[o.RIGHT]={top:e,right:0},r[o.LEFT]={top:e,left:0},r[o.CENTER]={width:i.width,height:i.height,objectFit:"none"},r}(l,h,t)[u])}return i}}},function(e,t,i){"use strict";var r=i(0),n=r.fittingTypes,o=r.alignTypes,a=r.transformTypes,s=i(1),c=s.getDimension,u=s.isImageTransformApplicable;e.exports={get:function(e,t){var i,r={css:{container:{}},attr:{container:{},img:{}}},s=r.css,l=r.attr,h=e.fittingType,d=t.alignment,f=e.src,p=f.width,g=f.height;switch(s.container.position="relative",h){case n.ORIGINAL_SIZE:case n.LEGACY_ORIGINAL_SIZE:case n.TILE:e.parts&&e.parts.length?(l.img.width=e.parts[0].width,l.img.height=e.parts[0].height):(l.img.width=p,l.img.height=g),l.img.preserveAspectRatio="xMidYMid slice";break;case n.SCALE_TO_FIT:case n.LEGACY_FIT_WIDTH:case n.LEGACY_FIT_HEIGHT:case n.LEGACY_FULL:l.img.width="100%",l.img.height="100%",l.img.transform="",l.img.preserveAspectRatio="";break;case n.STRETCH:l.img.width=t.width,l.img.height=t.height,l.img.x=0,l.img.y=0,l.img.transform="",l.img.preserveAspectRatio="none";break;case n.SCALE_TO_FILL:u(e.src.id)?(l.img.width=t.width,l.img.height=t.height):(i=c(p,g,t.width,t.height,a.FILL),l.img.width=i.width,l.img.height=i.height),l.img.x=0,l.img.y=0,l.img.transform="",l.img.preserveAspectRatio="xMidYMid slice"}if("number"==typeof l.img.width&&(l.img.width!==t.width||l.img.height!==t.height)){var m,b,v=0,T=0;h===n.TILE?(m=t.width%l.img.width,b=t.height%l.img.height):(m=t.width-l.img.width,b=t.height-l.img.height);var _=Math.round(m/2),y=Math.round(b/2);switch(d){case o.TOP_LEFT:v=0,T=0;break;case o.TOP:v=_,T=0;break;case o.TOP_RIGHT:v=m,T=0;break;case o.LEFT:v=0,T=y;break;case o.CENTER:v=_,T=y;break;case o.RIGHT:v=m,T=y;break;case o.BOTTOM_LEFT:v=0,T=b;break;case o.BOTTOM:v=_,T=b;break;case o.BOTTOM_RIGHT:v=m,T=b}l.img.x=v,l.img.y=T}return l.container.width=t.width,l.container.height=t.height,l.container.viewBox=[0,0,t.width,t.height].join(" "),r}}},function(e,t,i){"use strict";var r=i(0),n=r.fittingTypes,o=r.alignTypes,a=r.transformTypes,s=i(1),c=s.getDimension,u=s.isImageTypeSupported,l=s.isExternalUrl;e.exports={get:function(e,t){var i,r,s,h={css:{container:{},img:{}}},d=h.css,f=e.fittingType,p=t.alignment;switch(e.parts&&e.parts.length?(i=e.parts[0].width,r=e.parts[0].height):(i=e.src.width,r=e.src.height),d.img.display="block",d.container.position="relative",d.img.position="absolute",d.img.top="auto",d.img.right="auto",d.img.bottom="auto",d.img.left="auto",f){case n.ORIGINAL_SIZE:case n.LEGACY_ORIGINAL_SIZE:d.img.width=i,d.img.height=r;break;case n.SCALE_TO_FIT:case n.LEGACY_FIT_WIDTH:case n.LEGACY_FIT_HEIGHT:case n.LEGACY_FULL:s=c(i,r,t.width,t.height,a.FIT),Object.assign(d.img,s);break;case n.STRETCH:d.img.width=t.width,d.img.height=t.height;break;case n.SCALE_TO_FILL:var g=e.src.id;u(g)||l(g)?(d.img.width=t.width,d.img.height=t.height):(s=c(i,r,t.width,t.height,a.FILL),Object.assign(d.img,s),d.container.overflow="hidden")}if(d.img.width!==t.width||d.img.height!==t.height){var m=Math.round((t.height-d.img.height)/2),b=Math.round((t.width-d.img.width)/2);switch(p){default:case o.CENTER:d.img.top=m,d.img.left=b;break;case o.LEFT:d.img.left=0,d.img.top=m;break;case o.RIGHT:d.img.right=0,d.img.top=m;break;case o.TOP:d.img.left=b,d.img.top=0;break;case o.BOTTOM:d.img.left=b,d.img.bottom=0;break;case o.TOP_RIGHT:d.img.right=0,d.img.top=0;break;case o.TOP_LEFT:d.img.left=0,d.img.top=0;break;case o.BOTTOM_RIGHT:d.img.right=0,d.img.bottom=0;break;case o.BOTTOM_LEFT:d.img.left=0,d.img.bottom=0}}return h}}},function(e,t,i){"use strict";var r,n=i(0),o=n.alignTypes,a=n.fittingTypes,s=((r={})[o.CENTER]="50% 50%",r[o.TOP_LEFT]="0% 0%",r[o.TOP_RIGHT]="100% 0%",r[o.TOP]="50% 0%",r[o.BOTTOM_LEFT]="0% 100%",r[o.BOTTOM_RIGHT]="100% 100%",r[o.BOTTOM]="50% 100%",r[o.RIGHT]="100% 50%",r[o.LEFT]="0% 50%",r),c=Object.entries(s).reduce((function(e,t){var i=t[0];return e[t[1]]=i,e}),{}),u=[a.TILE,a.TILE_HORIZONTAL,a.TILE_VERTICAL,a.LEGACY_BG_FIT_AND_TILE,a.LEGACY_BG_FIT_AND_TILE_HORIZONTAL,a.LEGACY_BG_FIT_AND_TILE_VERTICAL],l=[a.LEGACY_ORIGINAL_SIZE,a.ORIGINAL_SIZE,a.LEGACY_BG_NORMAL];function h(e,t,i){var r=i.width,n=i.height;return e===a.TILE&&t.width>r&&t.height>n}function d(e,t,i){var r=e.width,n=e.height,o=t.width,a=t.height,s=i.x,c=i.y;if(!o||!a)return s+"% "+c+"%";var u=Math.max(o/r,a/n),l=r*u,h=n*u,d=Math.max(0,Math.min(l-o,l*(s/100)-o/2)),f=Math.max(0,Math.min(h-a,h*(c/100)-a/2));return(d&&Math.floor(d/(l-o)*100))+"% "+(f&&Math.floor(f/(h-a)*100))+"%"}e.exports.validateTargetDimensions=function(e,t){var i=t.width,r=t.height;if(!i||!r){var n=i||Math.min(980,e.width),o=n/e.width;return{width:n,height:r||e.height*o}}return{width:i,height:r}},e.exports.getScaledDimensions=function(e,t,i,r){var n=i.width,o=i.height;if(void 0===r&&(r=!1),r)return{width:n,height:o};var a=!l.includes(e),s=h(e,t,{width:n,height:o}),c=!s&&u.includes(e),d=c?t.width:n,f=c?t.height:o,p=a?function(e){return e>900?.25:e>500?.3:e>200?.4:1}(d):1;return{width:s?1920:d*p,height:f*p}},e.exports.getConvertedFitting=function(e,t){var i=u.includes(e)&&!t;return e===a.SCALE_TO_FILL||i?a.SCALE_TO_FIT:e},e.exports.getIsFakeTile=h,e.exports.getBlurValue=function(e,t,i){return i?0:u.includes(t)?1:e>200?2:3},e.exports.getCSSOverrides=function(e,t,i,r){void 0===r&&(r="center");var n,o,l={img:{},container:{}};if(e===a.SCALE_TO_FILL){var h=t.focalPoint&&(o=(n=t.focalPoint).x+"% "+n.y+"%",c[o]||""),f=h||r;t.focalPoint&&!h?l.img={objectPosition:d(t,i,t.focalPoint)}:l.img={objectPosition:s[f]}}else[a.LEGACY_ORIGINAL_SIZE,a.ORIGINAL_SIZE].includes(e)?l.img={objectFit:"none",top:"auto",left:"auto",right:"auto",bottom:"auto"}:u.includes(e)&&(l.container={backgroundSize:t.width+"px "+t.height+"px"});return l}}])},50343:function(){!function(){if(void 0!==window.Reflect&&void 0!==window.customElements&&!window.customElements.hasOwnProperty("polyfillWrapFlushCallback")){var e=HTMLElement;window.HTMLElement=function(){return Reflect.construct(e,[],this.constructor)},HTMLElement.prototype=e.prototype,HTMLElement.prototype.constructor=HTMLElement,Object.setPrototypeOf(HTMLElement,e)}}()},58699:function(e){"use strict";var t=function(e){return e*Math.PI/180},i=function(e,t){return{width:e,height:t}},r=function(e,t,i){return{width:e,height:Math.max(t,i)}};e.exports={BackgroundParallax:{hasParallax:!0,getMediaDimensions:r},BackgroundParallaxZoom:{hasParallax:!0,getMediaDimensions:r},BackgroundReveal:{hasParallax:!0,getMediaDimensions:r},BgCloseUp:{getMediaDimensions:i},BgExpand:{getMediaDimensions:i},BgFabeBack:{getMediaDimensions:i},BgFadeIn:{getMediaDimensions:i},BgFadeOut:{getMediaDimensions:i},BgFake3D:{hasParallax:!0,getMediaDimensions:r},BgPanLeft:{getMediaDimensions:function(e,t){return{width:1.2*e,height:t}}},BgPanRight:{getMediaDimensions:function(e,t){return{width:1.2*e,height:t}}},BgParallax:{hasParallax:!0,getMediaDimensions:r},BgPullBack:{getMediaDimensions:i},BgReveal:{hasParallax:!0,getMediaDimensions:r},BgRotate:{getMediaDimensions:function(e,i){return function(e,i,r){var n=t(r),o=Math.hypot(e,i)/2,a=Math.acos(e/2/o),s=e*Math.abs(Math.cos(n))+i*Math.abs(Math.sin(n)),c=e*Math.abs(Math.sin(n))+i*Math.abs(Math.cos(n));return{width:Math.ceil(n<a?s:2*o),height:Math.ceil(n<t(90)-a?c:2*o)}}(e,i,22)}},BgShrink:{getMediaDimensions:i},BgSkew:{getMediaDimensions:function(e,i){return function(e,i,r){var n=t(r);return{width:e,height:e*Math.tan(n)+i}}(e,i,20)}},BgUnwind:{getMediaDimensions:i},BgZoomIn:{hasParallax:!0,getMediaDimensions:r},BgZoomOut:{getMediaDimensions:function(e,t){return{width:1.15*e,height:1.15*t}}}}},17948:function(e,t,i){"use strict";var r=i(31191),n=i(96114),o=i.n(n),a=i(76702),s=i(21405),c=i(58699),u=i.n(c),l=i(46436),h=i.n(l),d=function(e,t,i){var r=(Array.isArray(t)?t:t.split(".")).reduce((function(e,t){return e&&void 0!==e[t]?e[t]:null}),e);return null!==r?r:i},f=function(e,t){return(Array.isArray(t)?t:[t]).reduce((function(t,i){var r,n=d(e,i);return void 0!==n?Object.assign(t,((r={})[i]=n,r)):t}),{})};function p(){for(var 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t=window.location.search.split("&").map((function(e){return e.split("=")})).find((function(e){return e[0].toLowerCase().includes("devicepixelratio")}));return(t?Number(t[1]):null)||e||1},v=function(e,t){return e.getAttribute(t?"xlink:href":"src")};function T(e){return e.isExperimentOpen("specs.thunderbolt.tb_stop_client_images")||e.isExperimentOpen("specs.thunderbolt.final_force_webp")||e.isExperimentOpen("specs.thunderbolt.final_force_no_webp")}var _={columnCount:1,columns:1,fontWeight:1,lineHeight:1,opacity:1,zIndex:1,zoom:1},y=function(e,t){return e&&t&&Object.keys(t).forEach((function(i){return e.setAttribute(i,t[i])}))},I=function(e,t){return e&&t&&Object.keys(t).forEach((function(i){var r=t[i];void 0!==r?e.style[i]=function(e,t){return"number"!=typeof t||_[e]?t:t+"px"}(i,r):e.style.removeProperty(i)}))},E=function(e,t){return e&&t&&Object.keys(t).forEach((function(i){e.style.setProperty(i,t[i])}))},A=function(e,t,i){return void 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[id="comp-kuzysdf0"]{position:relative;margin:0px 0px -26px calc((100% - 980px) * 0.5);left:526px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyg4wainlineContent-gridContainer] > [id="comp-kuzysym3"]{position:relative;margin:26px 0px 53px calc((100% - 980px) * 0.5);left:526px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyg4wainlineContent-gridContainer] > [id="comp-kv00lklc"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:628px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyvhebinlineContent]{height:auto;width:100%}[data-mesh-id=comp-kuzyvhebinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(2, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kuzyvhebinlineContent-gridContainer] > [id="comp-kuzyvhee"]{position:relative;margin:43px 0px 0 calc((100% - 980px) * 0.5);left:37px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyvhebinlineContent-gridContainer] > [id="comp-kuzyx3fh"]{position:relative;margin:0px 0px 20px calc((100% - 980px) * 0.5);left:37px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyvhebinlineContent-gridContainer] > [id="comp-kuzyvheh"]{position:relative;margin:0px 0px 44px calc((100% - 980px) * 0.5);left:37px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzyvhebinlineContent-gridContainer] > [id="comp-kuzyvhej"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:536px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzgk3tinlineContent]{height:auto;width:100%}[data-mesh-id=comp-kuzzgk3tinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:1fr;grid-template-columns:100%}[data-mesh-id=comp-kuzzgk3tinlineContent-gridContainer] > [id="comp-kuzzgk3y1"]{position:relative;margin:58px 0px 59px calc((100% - 980px) * 0.5);left:100px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(4, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00qzz61"]{position:relative;margin:34px 0px -51px calc((100% - 980px) * 0.5);left:31px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00qzze1"]{position:relative;margin:203px 0px 36px calc((100% - 980px) * 0.5);left:62px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00qzzc"]{position:relative;margin:0px 0px 43px calc((100% - 980px) * 0.5);left:62px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00st0a"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:62px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00qzz72"]{position:relative;margin:52px 0px 64px calc((100% - 980px) * 0.5);left:617px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv00qzz4inlineContent-gridContainer] > [id="comp-kv00qzz92"]{position:relative;margin:0px 0px 58px calc((100% - 980px) * 0.5);left:520px;grid-area:2 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent]{height:auto;width:100%}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(5, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5wz1"]{position:relative;margin:38px 0px 38px calc((100% - 980px) * 0.5);left:32px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5x4"]{position:relative;margin:64px 0px 64px calc((100% - 980px) * 0.5);left:116px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5x51"]{position:relative;margin:0px 0px 11px calc((100% - 980px) * 0.5);left:42px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5x63"]{position:relative;margin:0px 0px 23px calc((100% - 980px) * 0.5);left:42px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5x92"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:532px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzn5x81"]{position:relative;margin:0px 0px 15px calc((100% - 980px) * 0.5);left:42px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kuzzz7w5"]{position:relative;margin:0px 0px 29px calc((100% - 980px) * 0.5);left:42px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuzzn5wtinlineContent-gridContainer] > [id="comp-kv001v871"]{position:relative;margin:0px 0px 0px calc((100% - 980px) * 0.5);left:42px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv011gxninlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(6, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv01287s"]{position:relative;margin:50px 0px 66px calc((100% - 980px) * 0.5);left:42px;grid-area:1 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv021zii"]{position:relative;margin:0px 0px 13px calc((100% - 980px) * 0.5);left:42px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv012s7r"]{position:relative;margin:44px 0px 0 calc((100% - 980px) * 0.5);left:502px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv012875"]{position:relative;margin:0px 0px 33px calc((100% - 980px) * 0.5);left:509px;grid-area:2 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv014j91"]{position:relative;margin:0px 0px 24px calc((100% - 980px) * 0.5);left:514px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv011pu8"]{position:relative;margin:24px 0px 19px calc((100% - 980px) * 0.5);left:48px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv011pv0"]{position:relative;margin:0px 0px 52px calc((100% - 980px) * 0.5);left:48px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv014j9s"]{position:relative;margin:0px 0px -51px calc((100% - 980px) * 0.5);left:515px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv011gxninlineContent-gridContainer] > [id="comp-kv014jag"]{position:relative;margin:67px 0px 10px calc((100% - 980px) * 0.5);left:514px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuz1aplj1inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kuz1aplj1inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(2, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kuz1aplj1inlineContent-gridContainer] > [id="comp-kuz1apll"]{position:relative;margin:36px 0px -3px calc((100% - 980px) * 0.5);left:1px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuz1aplj1inlineContent-gridContainer] > [id="comp-kuz1aplq"]{position:relative;margin:0px 0px 23px calc((100% - 980px) * 0.5);left:1px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuz1aplj1inlineContent-gridContainer] > [id="comp-kuz1aplu"]{position:relative;margin:0px 0px 37px calc((100% - 980px) * 0.5);left:1px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kuz1aplj1inlineContent-gridContainer] > [id="comp-kuz1aply2"]{position:relative;margin:5px 0px 63px calc((100% - 980px) * 0.5);left:7px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent]{height:auto;width:100%}[data-mesh-id=ContainerxllhdinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:16832px;grid-template-rows:repeat(33, min-content) 1fr;grid-template-columns:100%;padding-bottom:20px;box-sizing:border-box}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1aphn"]{position:relative;margin:23px 0px 12px calc((100% - 980px) * 0.5);left:14px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apht"]{position:relative;margin:0px 0px 12px calc((100% - 980px) * 0.5);left:22px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1aphu1"]{position:relative;margin:0px 0px 59px calc((100% - 980px) * 0.5);left:22px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1my03"]{position:relative;margin:196px 0px 39px calc((100% - 980px) * 0.5);left:539px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1api3"]{position:relative;margin:0px 0px 28px calc((100% - 980px) * 0.5);left:544px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1api61"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apiu1"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apiq"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apj9"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:9 / 1 / 10 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz206e1"]{position:relative;margin:0px 0px -6px calc((100% - 980px) * 0.5);left:0px;grid-area:8 / 1 / 9 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apjp"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:10 / 1 / 11 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apk4"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:11 / 1 / 12 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apkk"]{position:relative;margin:0px 0px 12px calc((100% - 980px) * 0.5);left:0px;grid-area:12 / 1 / 13 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apkt"]{position:relative;margin:0px 0px 6px calc((100% - 980px) * 0.5);left:43px;grid-area:13 / 1 / 14 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apkv"]{position:relative;margin:16px 0px 0 calc((100% - 980px) * 0.5);left:43px;grid-area:14 / 1 / 15 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apkx"]{position:relative;margin:0px 0px 9px calc((100% - 980px) * 0.5);left:43px;grid-area:15 / 1 / 16 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz3vl6u"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:595px;grid-area:14 / 1 / 17 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apl1"]{position:relative;margin:1px 0px 10px calc((100% - 980px) * 0.5);left:43px;grid-area:17 / 1 / 18 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz494m6"]{position:relative;margin:0px 0px 20px calc((100% - 980px) * 0.5);left:507px;grid-area:17 / 1 / 18 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1apl21"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:18 / 1 / 19 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzx15ih"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:19 / 1 / 20 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzxd9a2"]{position:relative;margin:0px 0px -7px calc((100% - 980px) * 0.5);left:0px;grid-area:20 / 1 / 21 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzxjavp"]{position:relative;margin:0px 0px 17px calc((100% - 980px) * 0.5);left:8px;grid-area:21 / 1 / 22 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kv00jwrb"]{position:relative;margin:45px 0px 10px calc((100% - 980px) * 0.5);left:555px;grid-area:21 / 1 / 22 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzxky7c"]{position:relative;margin:0px 0px 36px calc((100% - 980px) * 0.5);left:18px;grid-area:22 / 1 / 23 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kv0095jl"]{position:relative;margin:0px 0px 21px calc((100% - 980px) * 0.5);left:19px;grid-area:23 / 1 / 24 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzxofid"]{position:relative;margin:0px 0px 7px calc((100% - 980px) * 0.5);left:19px;grid-area:24 / 1 / 25 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzxovk8"]{position:relative;margin:0px 0px 61px calc((100% - 980px) * 0.5);left:24px;grid-area:25 / 1 / 26 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzy6yx1"]{position:relative;margin:0px 0px 35px calc((100% - 980px) * 0.5);left:38px;grid-area:26 / 1 / 27 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1aplc"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:27 / 1 / 28 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzyg4uv"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:28 / 1 / 29 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzyvhcp"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:29 / 1 / 30 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzzgk2c"]{position:relative;margin:0px 0px 40px calc((100% - 980px) * 0.5);left:0px;grid-area:31 / 1 / 32 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kv00qzx3"]{position:relative;margin:0px 0px -14px calc((100% - 980px) * 0.5);left:0px;grid-area:30 / 1 / 31 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuzzn5v0"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:32 / 1 / 33 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kv011gvk"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:33 / 1 / 34 / 2;justify-self:start;align-self:start}[data-mesh-id=ContainerxllhdinlineContent-gridContainer] > [id="comp-kuz1aplh1"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:34 / 1 / 35 / 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[data-mesh-id=comp-kv13a79l1inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(18, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv13a79r1"]{position:relative;margin:31px 0px 16px calc((100% - 980px) * 0.5);left:1px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv147l7s"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:1px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv13v7co"]{position:relative;margin:0px 0px -31px calc((100% - 980px) * 0.5);left:539px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv13a79w2"]{position:relative;margin:43px 0px 48px calc((100% - 980px) * 0.5);left:539px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv13a79v"]{position:relative;margin:0px 0px 33px calc((100% - 980px) * 0.5);left:1px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv14j13n"]{position:relative;margin:0px 0px -27px calc((100% - 980px) * 0.5);left:1px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv14n3rc"]{position:relative;margin:41px 0px 10px calc((100% - 980px) * 0.5);left:6px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv151ldm"]{position:relative;margin:0px 0px 24px calc((100% - 980px) * 0.5);left:469px;grid-area:6 / 1 / 7 / 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[id="comp-kv162yhg"]{position:relative;margin:0px 0px 45px calc((100% - 980px) * 0.5);left:-4px;grid-area:11 / 1 / 12 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv1689yw"]{position:relative;margin:0px 0px 45px calc((100% - 980px) * 0.5);left:1px;grid-area:12 / 1 / 15 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv163eri"]{position:relative;margin:0px 0px 5px calc((100% - 980px) * 0.5);left:523px;grid-area:12 / 1 / 13 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv163es8"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:528px;grid-area:13 / 1 / 14 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a79l1inlineContent-gridContainer] > [id="comp-kv1698xy"]{position:relative;margin:0px 0px 38px calc((100% - 980px) * 0.5);left:1px;grid-area:15 / 1 / 16 / 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1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7afinlineContent-gridContainer] > [id="comp-kv13a7ag3"]{position:relative;margin:40px 0px 58px calc((100% - 980px) * 0.5);left:51px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7afinlineContent-gridContainer] > [id="comp-kv13a7ai"]{position:relative;margin:23px 0px 10px calc((100% - 980px) * 0.5);left:51px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7afinlineContent-gridContainer] > [id="comp-kv16l9k0"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:424px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16lixzinlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv16lixzinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(2, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv16lixzinlineContent-gridContainer] > [id="comp-kv16liy1"]{position:relative;margin:40px 0px 10px calc((100% - 980px) * 0.5);left:51px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16lixzinlineContent-gridContainer] > [id="comp-kv16liy22"]{position:relative;margin:0px 0px 21px calc((100% - 980px) * 0.5);left:51px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16lixzinlineContent-gridContainer] > [id="comp-kv16riz5"]{position:relative;margin:6px 0px 42px calc((100% - 980px) * 0.5);left:424px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16lixzinlineContent-wedge-3]{visibility:hidden;height:175px;width:0;grid-area:1 / 1 / 3 / 2}[data-mesh-id=comp-kv16rvoq1inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv16rvoq1inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:508px;grid-template-rows:min-content 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv16rvoq1inlineContent-gridContainer] > [id="comp-kv16rvos1"]{position:relative;margin:40px 0px 36px calc((100% - 980px) * 0.5);left:51px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16rvoq1inlineContent-gridContainer] > [id="comp-kv16rvou"]{position:relative;margin:45px 0px 10px calc((100% - 980px) * 0.5);left:51px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16rvoq1inlineContent-gridContainer] > [id="comp-kv16vm6y"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:424px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16vvll1inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv16vvll1inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:min-content 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv16vvll1inlineContent-gridContainer] > [id="comp-kv16vvln1"]{position:relative;margin:40px 0px 42px calc((100% - 980px) * 0.5);left:51px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16vvll1inlineContent-gridContainer] > [id="comp-kv16vvlo3"]{position:relative;margin:39px 0px 69px calc((100% - 980px) * 0.5);left:51px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv16vvll1inlineContent-gridContainer] > [id="comp-kv171mxd"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:430px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7b82inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a7b82inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:min-content 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7b82inlineContent-gridContainer] > [id="comp-kv13a7bb"]{position:relative;margin:42px 0px 6px calc((100% - 980px) * 0.5);left:124px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7b82inlineContent-gridContainer] > [id="comp-kv13a7bc2"]{position:relative;margin:0px 0px 68px calc((100% - 980px) * 0.5);left:53px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7brinlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a7brinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:min-content 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7brinlineContent-gridContainer] > [id="comp-kv13a7bt"]{position:relative;margin:32px 0px 16px calc((100% - 980px) * 0.5);left:44px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7brinlineContent-gridContainer] > [id="comp-kv13a7bu"]{position:relative;margin:0px 0px 28px calc((100% - 980px) * 0.5);left:26px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7brinlineContent-gridContainer] > [id="comp-kv13a7bw"]{position:relative;margin:19px 0px 69px calc((100% - 980px) * 0.5);left:110px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(4, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv13a7ay"]{position:relative;margin:66px 0px 0 calc((100% - 980px) * 0.5);left:523px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv13a7b02"]{position:relative;margin:0px 0px 13px calc((100% - 980px) * 0.5);left:523px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv13a7az"]{position:relative;margin:0px 0px 59px calc((100% - 980px) * 0.5);left:523px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv19ts7a"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:121px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv13a7b21"]{position:relative;margin:0px 0px 70px calc((100% - 980px) * 0.5);left:523px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7al2inlineContent-gridContainer] > [id="comp-kv13a7b5"]{position:relative;margin:0px 0px 0px calc((100% - 980px) * 0.5);left:1px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(10, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv13a7bk2"]{position:relative;margin:36px 0px 50px calc((100% - 980px) * 0.5);left:30px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv17qjmb"]{position:relative;margin:46px 0px 45px calc((100% - 980px) * 0.5);left:501px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv13a7bn1"]{position:relative;margin:0px 0px 22px calc((100% - 980px) * 0.5);left:594px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv1874s9"]{position:relative;margin:0px 0px 35px calc((100% - 980px) * 0.5);left:30px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18m2u1"]{position:relative;margin:0px 0px 41px calc((100% - 980px) * 0.5);left:30px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18o7az"]{position:relative;margin:0px 0px 40px calc((100% - 980px) * 0.5);left:37px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18qehf"]{position:relative;margin:0px 0px 45px calc((100% - 980px) * 0.5);left:363px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18ubqx"]{position:relative;margin:0px 0px 34px calc((100% - 980px) * 0.5);left:12px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18vgtl"]{position:relative;margin:0px 0px 32px calc((100% - 980px) * 0.5);left:372px;grid-area:8 / 1 / 9 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18v1ds"]{position:relative;margin:0px 0px 15px calc((100% - 980px) * 0.5);left:17px;grid-area:9 / 1 / 10 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18wf1d"]{position:relative;margin:0px 0px 8px calc((100% - 980px) * 0.5);left:29px;grid-area:10 / 1 / 11 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7bi1inlineContent-gridContainer] > [id="comp-kv18wf27"]{position:relative;margin:0px 0px 16px calc((100% - 980px) * 0.5);left:69px;grid-area:11 / 1 / 12 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv17gh58inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv17gh58inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:min-content 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv17gh58inlineContent-gridContainer] > [id="comp-kv17gh5a"]{position:relative;margin:32px 0px 16px calc((100% - 980px) * 0.5);left:44px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv17gh58inlineContent-gridContainer] > [id="comp-kv17gh5b1"]{position:relative;margin:0px 0px 17px calc((100% - 980px) * 0.5);left:26px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv17gh58inlineContent-gridContainer] > [id="comp-kv17gh5e2"]{position:relative;margin:34px 0px 52px calc((100% - 980px) * 0.5);left:110px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent]{height:auto;width:100%}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:auto;grid-template-rows:repeat(10, min-content) 1fr;grid-template-columns:100%}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv13a7c2"]{position:relative;margin:41px 0px 27px calc((100% - 980px) * 0.5);left:48px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv13a7c3"]{position:relative;margin:0px 0px 36px calc((100% - 980px) * 0.5);left:48px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv194vl9"]{position:relative;margin:52px 0px 49px calc((100% - 980px) * 0.5);left:519px;grid-area:1 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv195ktz"]{position:relative;margin:0px 0px 27px calc((100% - 980px) * 0.5);left:48px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv199a82"]{position:relative;margin:0px 0px -2px calc((100% - 980px) * 0.5);left:48px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv195kuv"]{position:relative;margin:0px 0px 40px calc((100% - 980px) * 0.5);left:48px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv1985un"]{position:relative;margin:39px 0px 10px calc((100% - 980px) * 0.5);left:514px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv198qb7"]{position:relative;margin:0px 0px 15px calc((100% - 980px) * 0.5);left:48px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv19cdfp"]{position:relative;margin:0px 0px 29px calc((100% - 980px) * 0.5);left:48px;grid-area:8 / 1 / 9 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv198qc6"]{position:relative;margin:0px 0px 13px calc((100% - 980px) * 0.5);left:48px;grid-area:9 / 1 / 10 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv19dc1h"]{position:relative;margin:0px 0px 24px calc((100% - 980px) * 0.5);left:48px;grid-area:10 / 1 / 11 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv19dc2f"]{position:relative;margin:19px 0px 57px calc((100% - 980px) * 0.5);left:48px;grid-area:11 / 1 / 12 / 2;justify-self:start;align-self:start}[data-mesh-id=comp-kv13a7c0inlineContent-gridContainer] > [id="comp-kv19fjyy"]{position:relative;margin:0px 0px 48px calc((100% - 980px) * 0.5);left:471px;grid-area:11 / 1 / 12 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent]{height:auto;width:100%}[data-mesh-id=Containersw3ajinlineContent-gridContainer]{position:static;display:grid;height:auto;width:100%;min-height:12434px;grid-template-rows:repeat(18, min-content) 1fr;grid-template-columns:100%;padding-bottom:20px;box-sizing:border-box}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a77z"]{position:relative;margin:23px 0px 15px calc((100% - 980px) * 0.5);left:14px;grid-area:1 / 1 / 2 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a78g"]{position:relative;margin:0px 0px 42px calc((100% - 980px) * 0.5);left:19px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv19peot"]{position:relative;margin:0px 0px 55px calc((100% - 980px) * 0.5);left:509px;grid-area:2 / 1 / 3 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a78x"]{position:relative;margin:0px 0px 28px calc((100% - 980px) * 0.5);left:544px;grid-area:3 / 1 / 4 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a791"]{position:relative;margin:0px 0px 10px calc((100% - 980px) * 0.5);left:0px;grid-area:4 / 1 / 5 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a79z"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:5 / 1 / 6 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7ad"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:6 / 1 / 7 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv16liwb"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:7 / 1 / 8 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv16rvn4"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:8 / 1 / 9 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv16vvjz"]{position:relative;margin:0px 0px 2px calc((100% - 980px) * 0.5);left:0px;grid-area:9 / 1 / 10 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7b6"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:0px;grid-area:10 / 1 / 11 / 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[id="comp-kv13a7by"]{position:relative;margin:0px 0px 12px calc((100% - 980px) * 0.5);left:0px;grid-area:15 / 1 / 16 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7c71"]{position:relative;margin:0px 0px 22px calc((100% - 980px) * 0.5);left:43px;grid-area:16 / 1 / 17 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7c9"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:43px;grid-area:17 / 1 / 18 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7ca2"]{position:relative;margin:0px 0px 37px calc((100% - 980px) * 0.5);left:43px;grid-area:18 / 1 / 19 / 2;justify-self:start;align-self:start}[data-mesh-id=Containersw3ajinlineContent-gridContainer] > [id="comp-kv13a7cd1"]{position:relative;margin:0px 0px 0 calc((100% - 980px) * 0.5);left:43px;grid-area:19 / 1 / 20 / 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255);width:100%;--column-width:980px;--column-flex:980 }#comp-kv13a7c0 { --bg-overlay-color:rgb(255, 255, 255);width:100%;--column-width:980px;--column-flex:980 }#pageBackground_sw3aj { --bg-position:absolute;--bg-overlay-color:rgb(var(--color_11));--fill-layer-background-overlay-color:transparent;--fill-layer-background-overlay-position:absolute }
  
 
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</style></pages-css>
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<div id="site-root"><div id="masterPage" class="mesh-layout">
 
<div id="site-root"><div id="masterPage" class="mesh-layout">
 
 
<main id="PAGES_CONTAINER" tabindex="-1"><div id="SITE_PAGES"><div class="_2YGAo"><div id="xllhd" class="_1FOTJ _3LL-w"><div class="_3jgRX"></div><div class="_2ufc7"><div id="Containerxllhd" class="_1KV2M"><div data-mesh-id="ContainerxllhdinlineContent" data-testid="inline-content" class=""><div data-mesh-id="ContainerxllhdinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1aphn" class="_1Q9if" data-testid="richTextElement"><h1 class="font_0" style="font-size:60px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold">Integrated Human Practices</span></h1></div><div id="comp-kuz1apht" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold;"><span style="color:#45ACFF">Overview</span></span></h2></div><div id="comp-kuz1aphu1" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">Access to safe water is a fundamental Human Right for a dignified life; Unfortunately many people in Mexico lack this precious resource. According to the Beverage Marketing Corporation, last year consumption of bottled water in Mexico was almost 10 million gallons, representing 9% of the global volume. In addition, the per capita consumption was 74.4 gallons, positioning the country in the first place worldwide in this classification [1]. One of the main reasons for this high consumption is the poor quality of the supply service and the inadequate management of water resources, that is why a significant number of people choose to buy bottled water, which is affordable and easy to get [2]; however, there is a widespread unawareness of the substances that might be present in bottled water and the health problems they could cause. For instance, these substances are the endocrine disruptive compounds (EDCs), which can be found in the microplastic particles generated through the abrasion of the plastic; thus, they are commonly present in food and beverages. The EDCs are used in the production of many plastic products, and it has been shown that they can mimic different hormones, interfering with hormone-based signals in the body (read more <span style="text-decoration:underline"><a href="https://2021.igem.org/Team:TecCEM/Description" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold">here)</span></a></span>.</span></p></div><div id="comp-kuz1my03" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuz1my03" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kuz1my03&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:2520,&quot;height&quot;:1393,&quot;uri&quot;:&quot;d211b2_a651f75110b147119538ae295a07256d~mv2.jpeg&quot;,&quot;name&quot;:&quot;pexels-photo-327090.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_a651f75110b147119538ae295a07256d~mv2.jpeg/v1/fill/w_551,h_305,al_c,q_80,usm_0.66_1.00_0.01/pexels-photo-327090_jpeg_auto%3Dcompress%26cs%3Dtinysrgb%26dpr%3D2%26h%3D750%26w%3D1260.webp"><img src="https://images.pexels.com/photos/327090/pexels-photo-327090.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-327090.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width:441px;height:244px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kuz1api3" class="_2UdPt"></div><section id="comp-kuz1api61" class="_3d64y"><div id="bgLayers_comp-kuz1api61" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1api61" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apia1" class="_1vNJf"><div id="bgLayers_comp-kuz1apia1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apia1" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apia1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apia1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1apik" class="_2UdPt"></div><div id="comp-kuz1q9te" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuz1q9te" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kuz1q9te&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:2250,&quot;height&quot;:1500,&quot;uri&quot;:&quot;d211b2_45ac599e3cb344529e9c52c716fa2814~mv2.jpeg&quot;,&quot;name&quot;:&quot;pexels-photo-3738388.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_45ac599e3cb344529e9c52c716fa2814~mv2.jpeg/v1/fill/w_525,h_350,al_c,q_80,usm_0.66_1.00_0.01/pexels-photo-3738388_jpeg_auto%3Dcompress%26cs%3Dtinysrgb%26dpr%3D2%26h%3D750%26w%3D1260.webp"><img src="https://images.pexels.com/photos/3738388/pexels-photo-3738388.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-3738388.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width:420px;height:280px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kuz1apin" class="_2UdPt"></div><div id="comp-kuz1apio" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We believe that an important part of the problem, regarding bottled water consumption, is that many people don’t know about the existence of microplastics and EDCs, because there is not much information at their reach on this matter. One of the reasons being that, to our knowledge, there is no quick and reliable method to quantify them. Considering this and acknowledging that it would be very difficult to meaningfully dimmish the bottled water consumption in our community, we decided to design a biosensor to detect the presence of EDCs in water, with the aim of raising awareness about them and determine preventive actions to reduce their intake (read more <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Description" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span></a></span>).</span></p>
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<main id="PAGES_CONTAINER" tabindex="-1"><div id="SITE_PAGES"><div class="_2YGAo"><div id="sw3aj" class="_1FOTJ _3LL-w"><div class="_3jgRX"></div><div class="_2ufc7"><div id="Containersw3aj" class="_1KV2M"><div data-mesh-id="Containersw3ajinlineContent" data-testid="inline-content" class=""><div data-mesh-id="Containersw3ajinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a77z" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h1 class="font_0" style="font-size:60px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold" id="primer">ZETASIZER</span></h1></div><div id="comp-kv13a78g" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">
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In this section we weill discuss the fact that <strong>the most consumed brand in central Mexico is the one with the highest concentration of microparticles</strong> thoughout the experiments we carried out, our analysis and collaborations. So, with the aim of detecting the presence of nano and microparticles in bottled water for determining their intake by consumers, we used the Zetasizer device at our university to carry out an analysis of water samples from bottles of different brands at the conditions presented below:</span></span></p>
  
<p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
  
<p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">A fundamental part throughout the development of the biosensor were our Human Practices, as all the activities we performed gave us insights to improve and helped us shape our project. For instance, we reached several experts through interviews or consultancies, who gave us feedback that allowed us to identify areas of opportunity. Furthermore, we collaborated with the Cántaro Azul non-profit organization to help them fulfill their mission of providing safe water to rural communities. The interviews we gave were also a great opportunity to participate in diffusion forums, like public radio and talk about our project, as well as the problem of the EDCs on a national scale. Moreover, we conducted surveys to know people’s plastic consumption habits and have a better understanding about the dimension of the problem. Additionally, our legislative framework analysis allowed us to identify a regulation, in which the maximum permissible limits of EDCs and microplastics could be included.</span></p></div><div data-mesh-id="comp-kuz1apia1inlineContent-wedge-3"></div><div data-mesh-id="comp-kuz1apia1inlineContent-wedge-5"></div></div></div></div></div></section><section id="comp-kuz1apiu1" class="_3d64y"><div id="bgLayers_comp-kuz1apiu1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apiu1" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apiw2" class="_1vNJf"><div id="bgLayers_comp-kuz1apiw2" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apiw2" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apiw2inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apiw2inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1apj0" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size: 37px; text-align: center;"><span style="font-size:37px;"><span class="color_14"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold">Cántaro Azul: A Funding through Webinars</span></span></span></h2></div><div id="comp-kuz1apj2" class="_2UdPt"></div><div id="comp-kuz1ug82" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="color:#45ACFF">Why did we do this?</span></span></h2></div><div id="comp-kuz1apj5" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">In Mexico, there is a large inequality gap in access to adequate and appropriate water, and sanitation services, as well as in the pursuit of healthy environments.<br>
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<ul class="font_8" style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light; font-size:17px; text-align:justify">
This gap is supported by the fact that in rural areas, only 42.6% have water every day with an exclusive toilet and connection to the drainage network or septic tank [3]&nbsp;</span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Condition A: No treatment (water bottles as purchased)</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Condition B: 37°C for 7 days</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Condition C: Sun radiation for 7 days ~ 35-38°C</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Condition D: Impact test*</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Condition E: Agitation Test for 7 days at oscillatory agitation (250 RPM)*.</span></span></p>
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</ul>
  
<p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span class="wixGuard">​</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
  
<p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">Another fact is that only 14% of indigenous multigrade and community schools have some means to ensure that children have access to water for human consumption. Also, it is estimated that, in Mexico, only 43% of the population has safe water [4]&nbsp; and according to data from the INEGI Census of Schools, Teachers and Students of Basic Education, there are around 25 schools in Mexico 1.7 million students that do not have access to a public water network</span></p></div><div id="comp-kuz1apj7" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">These figures show the impact of the problem existing in Mexico regarding water sanitation and which is directly involved in the cause of diseases. This reduces the possibility of enjoying the human right to water and sanitation for the rural populations of Mexico [5] Recognizing this problem, we decided to collaborate with Cantaro Azul through which we sought to contribute to the achievement of Objective 6 (“Clean water and sanitation”) of the Sustainable Development Goals.</span></p>
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<p class="font_8" style="font-size:14px; text-align:justify"><span style="font-size:14px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">*These conditions weren’t applied to the glass and aluminum bottles</span></span></p>
  
<p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span class="wixGuard">​</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
  
<p class="font_7" style="text-align:justify;font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">As part of this collaboration, we offered a series of webinars that gave participants an overview of the impact of plastic on the environment, the effect of EDCs on different diseases ,such as obesity and the role of these plastics in the Mexican industry. The objective of this was to raise awareness about the impact of plastic on daily life. These webinars were open to the public and had a cost of $3 USD and incentives to the community of our university.</span></p></div><div data-mesh-id="comp-kuz1apiw2inlineContent-wedge-5"></div></div></div></div></div></section><section id="comp-kuz1apiq" class="_3d64y"><div id="bgLayers_comp-kuz1apiq" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apiq" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apir2" class="_1vNJf"><div id="bgLayers_comp-kuz1apir2" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apir2" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apir2inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apir2inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1xe4g" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="color:#45ACFF">What did we learn?&nbsp;</span></span></h2></div><div id="comp-kuz1apit1" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">The fact that we organized these webinars so that members of our society could participate was of great importance, as we raised awareness about this huge problem for our colleagues, students and members of our community. We also learned a lot ourselves; specially about the needs of our world regarding plastic and its correct disposal.</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The studied water bottles were from the following brands:</span></span></p>
  
<p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">In addition, it was achieved to have the knowledge of how EDCs cause different diseases including diabetes, which has been one of the most impactful diseases in the last few years. Given that Mexico occupies the first place in obesity, this webinar was useful to spread information about this disease. It involves not only genetic factors, but is generated by an accumulation of various other external variables that trigger it.</span></span><br>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
&nbsp;</p></div></div></div></div></div></section><section id="comp-kuz1apj9" class="_3d64y"><div id="bgLayers_comp-kuz1apj9" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apj9" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apjc1" class="_1vNJf"><div id="bgLayers_comp-kuz1apjc1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apjc1" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apjc1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apjc1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz3h6ah" class=""><div data-testid="svgRoot-comp-kuz3h6ah" class="_3bLYT _2OIRR"><svg preserveAspectRatio="xMidYMid meet" data-bbox="49.489 25.542 101.026 148.943" viewBox="49.489 25.542 101.026 148.943" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="color" role="presentation" aria-hidden="true"><defs><style>#comp-kuz3h6ah svg [data-color="1"] {fill: #45ACFF;}</style></defs>
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</div></div><div id="comp-kuz1apjg" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:23px"><span style="font-size:23px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">Particle analysis in bottled water for collaboration with Cántaro Azul</span></span></span></h2></div><div id="comp-kuz1apjj" class="_2UdPt"></div><div id="comp-kuz39g6j" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:23px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="font-size:23px"><span style="color:#001489">Why did we do this?</span></span></span></h2></div><div id="comp-kuz1apjl" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">A very important part of our project was to determine the intake of microplastics by people who consume bottled water, so we used the Zeta Sizer device from our university to analyze the presence of nano and microparticles in bottled water samples from various brands at different conditions, like sun exposure and impact (read more <span style="font-weight:bold;"><span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Results_Zetasizer" target="_blank" rel="noreferrer noopener">here</a></span></span>). This would help us to have a better idea of the EDCs consumption, as these substances are frequently used in the manufacturing of a wide range of plastic products; thus, being present within the majority of microplastics.</span></span></p></div><div id="comp-kuz1apjn1" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kuz206e1" class="_3d64y"><div id="bgLayers_comp-kuz206e1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz206e1" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz206ff" class="_1vNJf"><div id="bgLayers_comp-kuz206ff" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz206ff" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz206ffinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz206ffinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz20tip" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="text-align:right;font-size:40px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="color:#45ACFF">What was the impact of this activity on our project?</span></span></h2></div><div id="comp-kuz21d3q" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:20px"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">In addition to offering people webinars focused on providing knowledge and awareness about the impact of plastic on our lives, the money collected from the first round of webinars was donated directly to Cantaro Azul. We collected aboutt $40 USD. It may not seem as much but it was the first time we did it. We hope we will open more Webinar rounds in the future. We hope that the money we collected will be used by Cantaro Azul to continue supporting marginalized communities in Mexico where there is no access to clean, ready for human water.</span></span></p>
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<p class="font_8" style="font-size:20px"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">We will keep collaborating with this great organization in the future (to learn more about Cántaro Azul click <span style="text-decoration:underline;"><a href="https://www.cantaroazul.org/" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold">here</span></a>)</span>.</span></span><br>
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<ul class="font_8" style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light; font-size:17px; text-align:justify">
&nbsp;</p></div></div></div></div></div></section><section id="comp-kuz1apjp" class="_3d64y"><div id="bgLayers_comp-kuz1apjp" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apjp" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apjr" class="_1vNJf"><div id="bgLayers_comp-kuz1apjr" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apjr" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apjrinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apjrinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1apjt" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">This information would also be helpful to the Cántaro Azul organization, since they aim to provide safe water and improve the health of people living in rural communities of Mexico. Specifically, many of their programs operate through water disinfection and purification systems, promoting at the same time water consumption, hygiene, and nutrition habits [6]. For the development of such programs, they commonly use plastic water jugs to provide and store water, which are constantly washed and reused. This constitutes a good practice to ensure access to safe water for the communities; however, the organization is unaware of the microplastics and the EDCs problem and how the multiple reuses and the sun exposure of the jugs could contribute to the particle liberation in water. For that reason, the results we obtained from the water samples analysis at our Campus will be shared with them, so that they know the conditions that could make the water jugs unsuitable for use due to the presence of microplastics and EDCs, which could have a negative impact on people’s health.</span></span></p>
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<li>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #1</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #2</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #3</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #4</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #5</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #6</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #7</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #8</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #9</span></span></p>
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</ul>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Furthermore, even though we analyzed water from plastic bottles, many of the brands also manufacture the 20 L (5.28 gal) plastic jugs that the organization uses, so the data we share might give them some insights on which condition has a major effect on each brand’s jugs.</span></span></p></div><div id="comp-kuz3ld6r" class=""><div data-testid="svgRoot-comp-kuz3ld6r" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="10.2 22 180.709 131.6" viewBox="10.2 22 180.709 131.6" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">To make a comparison between the particles liberated in water from bottles of different materials, some of these bottles were made of plastic and others were made of glass and aluminum.</span></span></p>
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        <path d="M175.6 78.2h-4.9c5.6-4 9.2-10.6 9.2-18 0-12.3-10-22.2-22.2-22.2-12.3 0-22.2 10-22.2 22.2 0 7.4 3.6 14 9.2 18H137c-2.8-6-8.8-10.1-15.8-10.1H114c7-4.4 11.6-12.3 11.6-21.1 0-13.8-11.2-25-25-25s-25 11.2-25 25c0 8.9 4.6 16.7 11.6 21.1h-6.7c-7 0-13 4.2-15.8 10.1h-8.4c5.6-4 9.2-10.6 9.2-18 0-12.3-10-22.2-22.2-22.2C31 38 21.1 48 21.1 60.2c0 7.4 3.6 14 9.2 18h-4.5c-8.6 0-15.6 7-15.6 15.6v56.6c0 1.8 1.5 3.2 3.2 3.2s3.2-1.5 3.2-3.2V93.8c0-5 4.1-9.1 9.1-9.1h37.4v43.9c0 1.8 1.5 3.2 3.2 3.2s3.2-1.5 3.2-3.2V85.5c0-6 4.9-10.9 10.9-10.9H121c6 0 10.9 4.9 10.9 10.9v43.1c0 1.8 1.5 3.2 3.2 3.2s3.2-1.5 3.2-3.2V84.7h37.1c5 0 9.1 4.1 9.1 9.1v56.6c0 1.8 1.5 3.2 3.2 3.2s3.2-1.5 3.2-3.2V93.8c.3-8.6-6.7-15.6-15.3-15.6zM43.3 75.9c-8.7 0-15.8-7.1-15.8-15.8s7.1-15.8 15.8-15.8 15.8 7.1 15.8 15.8S52 75.9 43.3 75.9zm57.3-10.4c-10.2 0-18.5-8.3-18.5-18.5s8.3-18.5 18.5-18.5 18.5 8.3 18.5 18.5-8.2 18.5-18.5 18.5zm57.1 10.4c-8.7 0-15.8-7.1-15.8-15.8s7.1-15.8 15.8-15.8 15.8 7.1 15.8 15.8-7.1 15.8-15.8 15.8z"></path>
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</div></div><div id="comp-kuz1apk2" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kuz1apk4" class="_3d64y"><div id="bgLayers_comp-kuz1apk4" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apk4" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apk6" class="_1vNJf"><div id="bgLayers_comp-kuz1apk6" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apk6" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apk6inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apk6inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz3muxg" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What did we learn?</span></span></span></h2></div><div id="comp-kuz1apk8" class=""><div data-testid="svgRoot-comp-kuz1apk8" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 69 160 62" viewBox="20 69 160 62" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
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        <path d="M166.554 131H33.446C26.094 131 20 124.984 20 117.513V82.487C20 75.113 25.998 69 33.446 69h133.108C173.906 69 180 75.016 180 82.487v35.027c0 7.47-6.094 13.486-13.446 13.486z"></path>
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</div></div><div id="comp-kuz1apki" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:center"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_12"><span style="font-size:17px">The results we got from the analysis with the Zeta Sizer and the determinations we made about the factors that could cause a change in the dimension and the distribution of plastic particles in bottled water form different brands, helped us to have a reference of the possible microplastics daily intake and thus the approximate EDCs concentration (read more <span style="font-weight:bold"><span style="text-decoration:underline"><a href="https://2021.igem.org/Team:TecCEM/Results_Zetasizer" target="_blank" rel="noreferrer noopener">here</a></span></span>).<br>
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<br>
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Moreover, by working with the organization and getting to know more about their programs, not only did we learn about their practices to maintain water purification systems and the importance of plastic water jugs to perform this activity, but we also got aware of the bottled water consumption among the communities in which the organization has presence; for example, as part of the “Agua Segura en Escuelas” program, Cántaro Azul has noticed that children in schools buy many water bottles, as they don’t have access to potable water or it is of poor quality and cannot be consumed. In the words of the organization: “the drinking water networks do not guarantee the quality of water for human consumption, due to poor disinfection, purification, storage and distribution practices…many families must choose between drinking non-potable water…or purchasing bottled purified water offered by the private sector” [7].<br>
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This situation has significantly increased the bottled water consumption of children and their families, which, besides being an expensive option, could affect their health because of the microplastics intake. Contemplating this, we understood how the organization tries to provide safe and cheap water by constantly refilling big plastic jugs; however, the conditions in which such jugs are stored and used must be considered, that is why we think that sharing our analysis and information with them, might be useful to improve their practices and achieve their goal of providing safe water to the communities.</span></span></span></h2></div></div></div></div></div></section><section id="comp-kuz1apkk" class="_3d64y"><div id="bgLayers_comp-kuz1apkk" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apkk" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apkm" class="_1vNJf"><div id="bgLayers_comp-kuz1apkm" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apkm" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apkminlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apkminlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz3qbvq" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="color:#001489;"><span style="font-size:29px"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What was the impact of this activity on our project?</span></span></span></h2></div><div id="comp-kuz1apkn2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The Zeta Sizer data contributed to confirm the presence of microplastics in bottled water samples and helped us to determine how different conditions have a particular effect in the number of microplastics found in water samples from distinct brands. This was very important to us, since it gave us a starting point to estimate the concentration of EDCs we might detect with our biosensor, depending on the conditions of the sample we analyze. In addition, based on this information, we can establish certain preventive measures to reduce the intake of microplastics (and therefore of EDCs), at least due to the bottled water consumption (read more <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Results" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span></a></span>). Also, as we previously stated, all of that could be useful as well to enhance Cántaro Azul’s usage of the water jugs.</span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span>On the other hand, as we found in literature [8], one of the problems of the Mexican population regarding water consumption is that people do not trust the quality of water, so they rather buy bottled water. On this matter, the collaboration with Cántaro Azul by knowing about their work and the strategies they implement in rural communities, made us realize that such problem is even greater in these locations, where the access to potable water is very limited.</span></span></span></p></div><div id="comp-kuz1apkr" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We know that there are many mechanisms to purify water from several contaminants, but, to our knowledge, none of them help to remove the EDCs derived from the microplastic particles (at least none of the methods available in our country), so this was one of the reasons we reconsidered the initial idea of our project, which consisted only in detecting the EDCs present in water, but not in degrading them. Therefore, we decided to start looking for effective ways of eliminating these substances and we came up with the idea of using the laccase enzyme because of its oxidative capabilities against micropollutants and the possibility to produce it on large scale (read more <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Results" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span></a></span>); nevertheless, even though we have performed several experiments for the laccase expression in E. coli, it wouldn’t yet be feasible to implement this part of the project for drinking water purification, as we still haven’t quite developed the methodologies to do so.&nbsp;</span></span></span></p></div></div></div></div></div></section><div id="comp-kuz1apkt" class="_2UdPt"></div><div id="comp-kuz1apkv" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:40px"><span style="color:#45ACFF"><span style="font-family:wfont_093ca4_4dcfda9e6d1242458c6bd410b0b78ced,wf_4dcfda9e6d1242458c6bd410b,orig_nunito_regular">Radio interviews</span></span></h2></div><div id="comp-kuz1apkx" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_14"><span style="font-size:17px"><span style="font-weight:bold">1. Radio Big Bang&nbsp;</span><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The results and the conditions applied for each bottle are shown in the graphs presented below (the X axis represents the size of the particles in nm and the Y axis represents the intensity of the signal in percentage):</span></span></p></div><div id="comp-kv19peot" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv19peot" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv19peot&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1000,&quot;height&quot;:1500,&quot;uri&quot;:&quot;d211b2_1350f56e8f1b44c899a27b7f06ec2e90~mv2.jpeg&quot;,&quot;name&quot;:&quot;pexels-photo-8217434.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_1350f56e8f1b44c899a27b7f06ec2e90~mv2.jpeg/v1/fill/w_559,h_839,al_c,q_85,usm_0.66_1.00_0.01/pexels-photo-8217434_jpeg_auto%3Dcompress%26cs%3Dtinysrgb%26dpr%3D2%26h%3D750%26w%3D1260.webp"><img src="https://images.pexels.com/photos/8217434/pexels-photo-8217434.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-8217434.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width:447px;height:671px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv13a78x" class="_2UdPt"></div><section id="comp-kv13a791" class="_3d64y"><div id="bgLayers_comp-kv13a791" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a791" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a79l1" class="_1vNJf"><div id="bgLayers_comp-kv13a79l1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a79l1" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a79l1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a79l1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a79r1" class="_2UdPt"></div><div id="comp-kv147l7s" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv147l7s" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv147l7s&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1599,&quot;height&quot;:902,&quot;uri&quot;:&quot;d211b2_acd5fb2b475b466dbc4fcc49765d801d~mv2.png&quot;,&quot;name&quot;:&quot;1599px-T--TecCEM--pp1.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_acd5fb2b475b466dbc4fcc49765d801d~mv2.png/v1/fill/w_578,h_326,al_c,q_85,usm_0.66_1.00_0.01/1599px-T--TecCEM--pp1_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/f/fe/T--TecCEM--pp1.jpeg/1599px-T--TecCEM--pp1.jpeg.png" alt="1599px-T--TecCEM--pp1.jpeg.png" style="width:462px;height:261px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv13v7co" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="color:#45ACFF" id="segundo">PET bottles<br>
<span style="font-weight:bold">With Leonardo Ferrera and Bárbara Schettino.</span></span></span></span><br>
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<span style="font-size:21px;">Brand #1</span></span></span></h2></div><div id="comp-kv13a79w2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Analysis of Brand #1 bottled water demonstrated the presence of particles at all conditions, even when no treatment was applied (condition A). Also, a change in particle dimensions and amount occurred when the bottle was subjected to heat (condition B); the same thing happened for the impact test (Condition D). With sun radiation (condition C) and agitation (condition E), two groups of particles of different sizes appeared, with a distinct distribution for each condition. This showed us that the conditions that had a major effect on the particles release were the solar radiation and agitation.&nbsp;</span></p></div><div id="comp-kv13a79v" class="_2UdPt"></div><div id="comp-kv14j13n" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="color:#45ACFF">Brand #2</span></span></h2></div><div id="comp-kv14n3rc" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">For water samples of Brand #2, large particles were detected in condition A; however, for conditions B and C there was presence of particles of smaller sizes, which indicated degradation. In condition D, two groups of particles appeared, but the larger group didn’t have a significant change in dimension respecting the particles from condition A. For condition E, two groups of particles were present as well, but with different dimensions and distribution than those from condition D. This revealed that heat,&nbsp; radiation and agitation affected most the particle liberation.</span></p></div><div id="comp-kv151ldm" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv151ldm" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv151ldm&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:904,&quot;uri&quot;:&quot;d211b2_0fd79b14330c48ee8e9323b234714ba6~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--epura.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_0fd79b14330c48ee8e9323b234714ba6~mv2.png/v1/fill/w_633,h_358,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--epura_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/4/4d/T--TecCEM--epura.jpeg/1600px-T--TecCEM--epura.jpeg.png" alt="1600px-T--TecCEM--epura.jpeg.png" style="width:506px;height:286px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv1523hy" class="_2UdPt"></div><div id="comp-kv155vmx" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="color:#45ACFF">Brand #3</span></span></h2></div><div id="comp-kv155vnq" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">In the water samples of Brand #3, two groups of particles were present in water for conditions A and C; nevertheless, they were different in distribution and amount, showing a change between the two conditions. The impact test performed for condition D only caused the appearance of one group of particles. For condition E, three groups of particles were detected, all of different amounts and sizes. This demonstrated that the particles’ distribution and amount changed when the bottle was subjected to treatment. For condition B, the device indicated “error”; thus, no measurements could be made.&nbsp;</span></p></div><div id="comp-kv16271p" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv16271p" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv16271p&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1599,&quot;height&quot;:906,&quot;uri&quot;:&quot;d211b2_cca809d07d384434bfac2380eddf6602~mv2.png&quot;,&quot;name&quot;:&quot;1599px-T--TecCEM--zoe.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_cca809d07d384434bfac2380eddf6602~mv2.png/v1/fill/w_633,h_358,al_c,q_85,usm_0.66_1.00_0.01/1599px-T--TecCEM--zoe_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/a/a9/T--TecCEM--zoe.jpeg/1599px-T--TecCEM--zoe.jpeg.png" alt="1599px-T--TecCEM--zoe.jpeg.png" style="width:506px;height:286px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv162yhg" class="_2UdPt"></div><div id="comp-kv1689yw" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv1689yw" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv1689yw&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:902,&quot;uri&quot;:&quot;d211b2_65ef20531c1b4bf88bcfa60260558fc2~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--great_value.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_65ef20531c1b4bf88bcfa60260558fc2~mv2.png/v1/fill/w_618,h_348,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--great_value_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/5/57/T--TecCEM--great_value.jpeg/1600px-T--TecCEM--great_value.jpeg.png" alt="1600px-T--TecCEM--great_value.jpeg.png" style="width:494px;height:278px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv163eri" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="color:#45ACFF">Brand #4</span></span></h2></div><div id="comp-kv163es8" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Analysis of Brand #4 bottled water showed presence of particles for every condition. For condition B, the particles were of a considerably smaller size than those from condition A, which implied degradation. Also, two groups of particles of different amounts and dimensions appeared for condition C, as well as for condition E. The particles detected in condition D were larger in size than most of the particles from the other conditions. This revealed a greater effect of sun radiation and agitation for the particle liberation.</span></p></div><div id="comp-kv1698xy" class="_2UdPt"></div><div id="comp-kv16aive" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="color:#45ACFF">Brand #5</span></span></h2></div><div id="comp-kv16aiwa" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align:justify;font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">No particles were detected in Brand #5 water samples for conditions A and D, which indicated a higher quality of the bottle; however, when heat was applied (Condition B), a group of particles appeared in water; the same thing happened when the bottle was subjected to agitation (condition E). The sun radiation exposure (Condition C) caused the liberation of even more particles, presenting three different groups of them; thus, being the major condition for particle generation.</span></p></div><div id="comp-kv16eai0" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv16eai0" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv16eai0&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:907,&quot;uri&quot;:&quot;d211b2_97fa7390f36f41079c84a09ade185519~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--fiji.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_97fa7390f36f41079c84a09ade185519~mv2.png/v1/fill/w_614,h_348,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--fiji_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/6/6a/T--TecCEM--fiji.jpeg/1600px-T--TecCEM--fiji.jpeg.png" alt="1600px-T--TecCEM--fiji.jpeg.png" style="width:491px;height:278px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv16eku9" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kv13a79z" class="_3d64y"><div id="bgLayers_comp-kv13a79z" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a79z" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7a11" class="_1vNJf"><div id="bgLayers_comp-kv13a7a11" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7a11" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7a11inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7a11inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7a32" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:37px; text-align:center"><span style="font-size:37px"><span class="color_14"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold" id="tercero">Glass and aluminium bottles</span></span></span></h2></div><div id="comp-kv13a7a5" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kv13a7ad" class="_3d64y"><div id="bgLayers_comp-kv13a7ad" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7ad" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7af" class="_1vNJf"><div id="bgLayers_comp-kv13a7af" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7af" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7afinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7afinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7ag3" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="color:#CDECA9"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="background-color:#A0A09F;">Brand #6</span></span></span></h2></div><div id="comp-kv13a7ai" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">Brand #6 water samples featured particles in all three conditions. Particularly, for condition A two groups of particles were detected and just one for conditions B and C. This was unexpected to us, since water is contained in a glass bottle; however, we believe that the plastic cap might be responsible for the particles’ release.</span></span></p></div><div id="comp-kv16l9k0" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv16l9k0" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv16l9k0&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:901,&quot;uri&quot;:&quot;d211b2_3e5776d57c0d4fba971eeb743b02ad4f~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--casa_del_agua.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_3e5776d57c0d4fba971eeb743b02ad4f~mv2.png/v1/fill/w_633,h_356,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--casa_del_agua_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/f/f8/T--TecCEM--casa_del_agua.jpeg/1600px-T--TecCEM--casa_del_agua.jpeg.png" alt="1600px-T--TecCEM--casa_del_agua.jpeg.png" style="width:506px;height:285px;object-fit:cover;object-position:50% 50%"></wix-image></div></div></div></div></div></div></section><section id="comp-kv16liwb" class="_3d64y"><div id="bgLayers_comp-kv16liwb" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16liwb" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv16lixz" class="_1vNJf"><div id="bgLayers_comp-kv16lixz" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16lixz" class="_2GUhU"></div></div><div data-mesh-id="comp-kv16lixzinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv16lixzinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv16liy1" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="color:#CDECA9"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="background-color:#A0A09F">Brand #7</span></span></span></h2></div><div id="comp-kv16liy22" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">The Brand #7 of water is presented in an aluminium container, the company stating themselves that the container is plastic free. Nonetheless, every metallic container needs a thin layer of protective coat, similar to soda cans. A polymer plastic lining is added to these cans to avoid the soda and aluminum reacting with each other. This may be the reason why this brand of water presents the same kind of nanoparticles throughout the tests.</span></span></p></div><div id="comp-kv16riz5" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv16riz5" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv16riz5&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:902,&quot;uri&quot;:&quot;d211b2_df5a270ae1434ffb9342d2b2e05c4a4b~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--rain.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_df5a270ae1434ffb9342d2b2e05c4a4b~mv2.png/v1/fill/w_633,h_356,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--rain_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/e/e8/T--TecCEM--rain.jpeg" alt="1600px-T--TecCEM--rain.jpeg.png" style="width:506px;height:285px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div data-mesh-id="comp-kv16lixzinlineContent-wedge-3"></div></div></div></div></div></section><section id="comp-kv16rvn4" class="_3d64y"><div id="bgLayers_comp-kv16rvn4" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16rvn4" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv16rvoq1" class="_1vNJf"><div id="bgLayers_comp-kv16rvoq1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16rvoq1" class="_2GUhU"></div></div><div data-mesh-id="comp-kv16rvoq1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv16rvoq1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv16rvos1" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="color:#CDECA9"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="background-color:#A0A09F">Brand #8</span></span></span></h2></div><div id="comp-kv16rvou" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">Brand #8 of water showed no particles throughout the three tests made. The principal reason this brand may not show any particles according to our study is because the water is contained in a glass bottle, not a plastic one. Demonstrating another great advantage of preferring glass over plastic, apart from is greater simplicity to be recycled.</span></span></p></div><div id="comp-kv16vm6y" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv16vm6y" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv16vm6y&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:902,&quot;uri&quot;:&quot;d211b2_cf31952ae0244c83a608c735a9635988~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--voss.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_cf31952ae0244c83a608c735a9635988~mv2.png/v1/fill/w_633,h_356,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--voss_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/b/bf/T--TecCEM--voss.jpeg/1600px-T--TecCEM--voss.jpeg.png" alt="1600px-T--TecCEM--voss.jpeg.png" style="width:506px;height:285px;object-fit:cover;object-position:50% 50%"></wix-image></div></div></div></div></div></div></section><section id="comp-kv16vvjz" class="_3d64y"><div id="bgLayers_comp-kv16vvjz" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16vvjz" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv16vvll1" class="_1vNJf"><div id="bgLayers_comp-kv16vvll1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv16vvll1" class="_2GUhU"></div></div><div data-mesh-id="comp-kv16vvll1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv16vvll1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv16vvln1" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="color:#CDECA9"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="background-color:#A0A09F">Brand #9</span></span></span></h2></div><div id="comp-kv16vvlo3" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:20px; text-align:justify"><span style="font-size:20px"><span style="font-family:roboto-thin,roboto,sans-serif">Brand #9 of water, just as Brand #8, showed no particles throughout the three tests made. The principal reason this brand may not show any particles according to our study is because the water is contained in a glass bottle, not a plastic one. Demonstrating another great advantage of preferring glass over plastic, apart from is greater simplicity to be recycled.</span></span></p></div><div id="comp-kv171mxd" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv171mxd" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv171mxd&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:901,&quot;uri&quot;:&quot;d211b2_826f7366ea304e0681eb6ed856e62691~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--evian.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_826f7366ea304e0681eb6ed856e62691~mv2.png/v1/fill/w_633,h_356,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--evian_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/thumb/8/8f/T--TecCEM--evian.jpeg/1600px-T--TecCEM--evian.jpeg.png" alt="1600px-T--TecCEM--evian.jpeg.png" style="width:506px;height:285px;object-fit:cover;object-position:50% 50%"></wix-image></div></div></div></div></div></div></section><section id="comp-kv13a7b6" class="_3d64y"><div id="bgLayers_comp-kv13a7b6" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7b6" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7b82" class="_1vNJf"><div id="bgLayers_comp-kv13a7b82" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7b82" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7b82inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7b82inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7bb" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="text-align:center;font-size:40px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="color:#45ACFF" id="cuarto">Overall analysis</span></span></h2></div><div id="comp-kv13a7bc2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size: 19px; text-align: justify;"><span style="font-size:19px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">Presence of particles was observed in all PET water bottles at most conditions. In every case, when subjected to heat or heat with solar radiation, a change in dimensions and distribution of the particles occurred. Specifically, a population of smaller particles appeared, which is related to degradation. Also, in general for all brands, no significant changes were observed in either quantity, distribution, or particle size for the impact test. Regarding the agitation test, more than one group of particles was detected for most cases, presenting particles of larger dimensions than those from the other conditions.</span></span></p>
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<span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="color_14"><span style="font-size:17px">Why did we do this?</span></span></span><br>
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<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_14"><span style="font-size:17px">This interview by the Radio Big Bang broadcaster, was to communicate and teach a little about our project. We talked about what synthetic biology is so that more people can have this information available and become interested in it. We exposed the problems we are addressing and how we are solving them through our project and our collaboration with organizations, other iGEM Teams and in general, with our community. Giving hard data and letting the audience know how dangerous endocrine disruptors are and how the lack of regulations regarding clean water and pollutants such as EDCs affects us as individuals.</span></span></span><br>
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<span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="color_14"><span style="font-size:17px">What did we learn?</span></span></span><br>
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<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_14"><span style="font-size:17px">We learned how to be more precise and how to expose our project and subject without a very technical language for every spectator to understand. We learned that a simple and clear explanation can be more meaningful than a long and boring one. Also, people are actually very interested in science and synthetic biology, especially when it’s their personal health. Finding a broadcaster that was interested in our project and interviewing us was a pleasant surprise and we hope that the diffusion of Synthetic Biology will help more people get interested in it.</span></span></span></h2></div><div id="comp-kuz3vl6u" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuz3vl6u" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kuz3vl6u&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1000,&quot;height&quot;:1500,&quot;uri&quot;:&quot;d211b2_27baaf4e67544edfb37dd3d4bed0ab3e~mv2.jpeg&quot;,&quot;name&quot;:&quot;pexels-photo-8132463.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_27baaf4e67544edfb37dd3d4bed0ab3e~mv2.jpeg/v1/fill/w_443,h_661,al_c,q_80,usm_0.66_1.00_0.01/pexels-photo-8132463_jpeg_auto%3Dcompress%26cs%3Dtinysrgb%26dpr%3D2%26h%3D750%26w%3D1260.webp"><img src="https://images.pexels.com/photos/8132463/pexels-photo-8132463.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-8132463.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width:354px;height:529px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kuz1apl1" class="_2UdPt"></div><div id="comp-kuz494m6" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="color_14"><span style="font-size:17px">What was the impact of this activity on our project?</span></span></span><br>
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<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_14"><span style="font-size:17px">The impact that it had was that many spectators in the live session, podcast in spotify and in Mix Cloud could acquire some digested information respecting EDCs and how we plan to face it. This common knowledge benefits us as a community since it creates an even more enriching consciousness. It is true that it is something worrisome, however we believe that concern leads to seeking solutions to the problem, encouraging other studies to focus on this branch of study and grow as a community. You can check out the interview we did on this link <span style="text-decoration:underline;"><a href="https://www.mixcloud.com/RadioBigBang/radio-big-bang-396-viernes-17-se-septiembre-2021/" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here.</span></a></span></span></span></span><br>
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&nbsp;</h2></div><section id="comp-kuz1apl21" class="_3d64y"><div id="bgLayers_comp-kuz1apl21" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apl21" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1apl41" class="_1vNJf"><div id="bgLayers_comp-kuz1apl41" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1apl41" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1apl41inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apl41inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1apl6" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size: 20px;"><span style="font-size:20px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span class="color_14">2. Ciudadana Radio 660 am with Rodrigo Reyes</span></span></span></h2></div><div id="comp-kuz1apl92" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="color_15"><span style="font-size:17px">Why did we do this?&nbsp;</span></span></span><br>
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<span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span class="color_15"><span style="font-size:17px">This radio program has a radio station called Terramar that touches on environmental issues so the interview served as a guideline to expose how microplastics affect the environment, to human health and in general to living beings giving an overview of the problems generated from these microplastics to the population listening to this radio channel and serving as a means of transmitting information and knowledge.</span></span></span><br>
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<br>
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<span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">What did we learn?</span><br>
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<span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">Conducting this interview helped us explain and raise awareness among people listening to this channel about how basic consumer products such as water bottles, juices and soft drinks have a huge health impact. In addition to understanding the types of regulations that exist for these products and in which part our project will be to these regulations.&nbsp;</span><br>
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<br>
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&nbsp;</h2></div><div id="comp-kuz1apla3" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span style="font-size:17px">What was the impact of this activity on our project?</span></span><br>
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<span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span style="font-size:17px">The problematic of EDCs toward human health is impactful, therefore, conducting this interview allowed people to become more aware of this situation, to which we are proposing an innovative solution. This is something that gave much diffusion to our project in the Mexican population.</span></span></h2></div><div id="comp-kuzx05ea" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuzx05ea" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kuzx05ea&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1080,&quot;height&quot;:1080,&quot;uri&quot;:&quot;d211b2_6f13933584d042c38dbeffd70805ee7c~mv2.jpg&quot;,&quot;name&quot;:&quot;T--TecCEM--Radio.jpg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_6f13933584d042c38dbeffd70805ee7c~mv2.jpg/v1/fill/w_330,h_330,al_c,q_80,usm_0.66_1.00_0.01/T--TecCEM--Radio.webp"><img src="https://static.igem.org/mediawiki/2021/4/4b/T--TecCEM--Radio.jpg" alt="T--TecCEM--Radio.jpg" style="width:264px;height:264px;object-fit:cover;object-position:50% 50%"></wix-image></div></div></div></div></div></div></section><section id="comp-kuzx15ih" class="_3d64y"><div id="bgLayers_comp-kuzx15ih" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzx15ih" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzx15kr" class="_1vNJf"><div id="bgLayers_comp-kuzx15kr" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzx15kr" class="_2GUhU"></div></div><div data-mesh-id="comp-kuzx15krinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzx15krinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzx3gkj" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:18px; text-align:justify"><span class="color_14"><span style="font-size:18px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">3. La autentik radio 92.5 FM with Candido Hernandez.</span></span></span><br>
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&nbsp;</p>
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<p class="font_8" style="font-size:18px; text-align:justify"><span class="color_14"><span style="font-size:18px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">Why did we do this?&nbsp;</span></span></span></p>
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<p class="font_8" style="font-size: 19px; text-align: justify;"><span style="font-size:19px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;"><span class="wixGuard"></span></span></span></p>
<br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="font-size:17px">This broadcaster reached out to us because they think that our project is a real catch. They are really interested in sharing science and projects related to students and young people. La Autentik is an important broadcaster in a town named Metzquititlán, in Hidalgo, Mexico. Candido Hernández was really excited for us to tell our project and share it in the town for other young people to start seeking and getting interested in science.&nbsp;</span></span></span><br>
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&nbsp;</p></div><div id="comp-kuzxaqpg" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuzxaqpg" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kuzxaqpg&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1001,&quot;height&quot;:536,&quot;uri&quot;:&quot;d211b2_afe1779a91a64cdb9032048f563a99c9~mv2.jpeg&quot;,&quot;name&quot;:&quot;T--TecCEM--Entrevista.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_afe1779a91a64cdb9032048f563a99c9~mv2.jpeg/v1/fill/w_574,h_308,al_c,q_80,usm_0.66_1.00_0.01/T--TecCEM--Entrevista.webp"><img src="https://static.igem.org/mediawiki/2021/d/dc/T--TecCEM--Entrevista.jpeg" alt="T--TecCEM--Entrevista.jpeg" style="width:459px;height:246px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kuzx5yk2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:18px; text-align:justify"><span class="color_14"><span style="font-size:18px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">What did we learn?</span></span></span></p><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Communicating our project effectively through language accessible to most of the Mexican population that is not necessarily related to a scientific way of talking was one of the things that this interview taught us. Besides, it was a refreshing experience to talk about motivating&nbsp; the population and giving exposure to the existing Mexican talent.&nbsp;</span></span></span></p><br>
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<p class="font_8" style="font-size: 19px; text-align: justify;"><span style="font-size:19px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">On the other hand, there was no presence of particles for two of the glass bottles (Brand #8 and Brand #9) at any condition. We hypothesize that particles detected in the remaining glass bottle (Brand #6) were due to the plastic bottle cap. Also, the presence of particles in the aluminum bottle water sample could be caused by some of the materials used in the bottle manufacturing.&nbsp;</span></span></p>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></p>
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<p class="font_8" style="font-size: 19px; text-align: justify;"><br>
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<span style="font-size:19px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">These results could be explained due to the effects of UV light, high temperatures and agitation on plastic. Particularly, we have found in literature that UV exposure can be an important source of plastic degradation, acting through autocatalytic thermal oxidation, and leading to an increase in the amount of microplastics and microfibers [1]. Furthermore, it has been shown that “plastic polymers have viscoelastic properties that can be altered by temperature…” [2]; in fact, hot water might exacerbate degradation, contributing to the release of microplastics [3]. Additionally, fragmentation of plastic is likely to occur because of agitational and mechanical forces, which might produce cracks in the material, especially if the bottle has been previously exposed to sunlight, becoming weak and brittle [4].</span></span></p>
  
<p class="font_8" style="font-size:18px; text-align:justify"><span class="color_14"><span style="font-size:18px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">What was the impact of this activity on our project?</span></span><br>
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<p class="font_8" style="font-size: 19px; text-align: justify;"><br>
<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We expanded our reach to other communities in Mexico. Hidalgo is a neighboring state to ours. We were happy to share our project and create plastic consciousness, for people to reduce their plastic consumption in other parts of the Republic.&nbsp;</span></span></span></p></div></div></div></div></div></section><section id="comp-kuzxd9a2" class="_3d64y"><div id="bgLayers_comp-kuzxd9a2" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzxd9a2" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzxd9bn" class="_1vNJf"><div id="bgLayers_comp-kuzxd9bn" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzxd9bn" class="_2GUhU"></div></div><div data-mesh-id="comp-kuzxd9bninlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzxd9bninlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzxd9c4" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size: 35px;"><span style="font-size:35px;"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black;">Legislative framework</span></span></span></h2></div><div id="comp-kuzxh976" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:35px"><span style="color:#45ACFF"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:35px">Overview</span></span></span></h2></div><div id="comp-kuzxd9c6" class="_2UdPt"></div><div id="comp-kv00ifug" class=""><div data-testid="svgRoot-comp-kv00ifug" class="_3bLYT _2OIRR"><!--?xml version="1.0" encoding="UTF-8"?-->
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<span style="font-size:19px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">All those findings will allow us to estimate the approximate intake of EDCs considering the amount of microplastics present in each sample, and determine which factors (temperature, sun exposure, brand, etc.) favor the liberation of these substances in bottled water.</span></span><br>
<svg preserveAspectRatio="xMidYMid meet" data-bbox="20.005 20 159.99 159.998" xmlns="http://www.w3.org/2000/svg" width="200" height="200" viewBox="20.005 20 159.99 159.998" data-type="color" role="presentation" aria-hidden="true"><defs><style>#comp-kv00ifug svg [data-color="1"] {fill: #242323;}</style></defs>
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&nbsp;</p></div></div></div></div></div></section><section id="comp-kv13a7bp" class="_3d64y"><div id="bgLayers_comp-kv13a7bp" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7bp" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7br" class="_1vNJf"><div id="bgLayers_comp-kv13a7br" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7br" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7brinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7brinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7bt" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">References:</span></span></span></h2></div><div id="comp-kv13a7bu" class=""><div data-testid="svgRoot-comp-kv13a7bu" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 69 160 62" viewBox="20 69 160 62" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
    <g>
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        <path fill-rule="evenodd" fill="#07073f" d="M162.687 43.394c4-1.152 6.257-2.361 12.315-3.168.485-.064 3.1-.343 3.279-.47.08-1.157-9.239-10.648-14.239-9.982-3.561.474-6.824 2.764-9.149 5.316a61.815 61.815 0 00-2.178-1.887c-14.107-11.562-30.644-11.4-40.027-2.412-8.033 7.689-4.142 26.965 9.326 39.1a13.947 13.947 0 00-3.186 7.531c-.3 4.828 9.77 13.047 10.909 12.885.113-.183.2-2.7.226-3.168a43.519 43.519 0 011.708-9.783c10.873 8.159 20.767 16.03 25.106 27.008 5.277 13.345 1.412 21.922-2.48 32.443-2.814.381-8.319 1.364-11.091 1.672-11.48 1.273-39.5 17.025-20.066 19.908l.458.069c5.137.758 18.44-3.734 20.285 3.172 2.7 10.109 5.061 17.878 16.025 18.37-1.4-10.168-9.164-29.655-5.314-38.545a1.145 1.145 0 00.01-.216 53.891 53.891 0 009.02-38.2 54.633 54.633 0 00-10.554-25.586 54.773 54.773 0 017.56 63.211c2.306-2.809 4.748-6.627 6.8-9.076 19.27-22.986 15.344-62.897-4.743-88.192z" data-color="1"></path>
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        <path fill-rule="evenodd" fill="#07073f" d="M77.986 130.108a13.942 13.942 0 003.186-7.532c.3-4.828-9.771-13.047-10.909-12.885-.113.183-.2 2.7-.226 3.168a43.519 43.519 0 01-1.708 9.783c-10.873-8.159-20.767-16.03-25.106-27.007-5.277-13.346-1.412-21.923 2.48-32.444 2.814-.381 8.319-1.364 11.09-1.671 11.481-1.273 39.5-17.02 20.067-19.909l-.458-.067c-5.137-.76-18.44 3.732-20.285-3.173C53.416 28.261 51.056 20.492 40.092 20c1.4 10.169 9.165 29.656 5.314 38.545a1.152 1.152 0 00-.01.217 53.888 53.888 0 00-9.02 38.2 54.645 54.645 0 0010.554 25.589 54.775 54.775 0 01-7.56-63.212c-2.306 2.809-4.748 6.626-6.8 9.076-19.27 22.985-15.344 62.897 4.743 88.191-4.005 1.153-6.257 2.361-12.315 3.168-.485.065-3.1.343-3.279.47-.08 1.158 9.239 10.648 14.239 9.982 3.561-.474 6.824-2.764 9.149-5.315a62.847 62.847 0 002.178 1.886c14.107 11.563 30.644 11.4 40.027 2.412 8.033-7.688 4.142-26.965-9.326-39.101z" data-color="1"></path>
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    </g>
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</svg>
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</div></div><div id="comp-kuzxd9cd" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">As we have mentioned before, plastic is not only an environmental issue, but a human and wildlife health matter. Summing up, the impact of the degradation of plastic polymers results in the accumulation of EDCs in water bodies that we as humans and marine life are highly exposed to [9] [10].<br>
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<span style="font-style:italic;">To see more information, visit the section of <span style="text-decoration:underline"><a href="https://drive.google.com/drive/u/1/folders/19OPHpqIK7ZoBCKgmqqmPlKA71j_RH0qQ" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold">SDG´s</span></a></span> where you can find more details about this health and environmental problems.</span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The focus we want to give to our “Disrupting Disruptors” project is to take it a step further and provide a reliable measurement and further degradation method. This is for industry to guarantee clean water and sanitation, health and wellness to every drinking water consumer as their human right and human basic need, as it states in National Commission for Human Rights.[11]</span></span></p></div><div id="comp-kuzxd9cg" class="_2UdPt"></div></div></div></div></div></section><div id="comp-kuzxjavp" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">There is a lack of quantifying methods for either microplastics and EDCs due to their size and difficulty to ensure reliable data through physical ways, making synthetic biology a striking and innovative option.&nbsp;<br>
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We found through literature, interviews and investigation the existence of a gap about normativities and regulations in Mexico when it comes to these both chemical compounds.</span></span></p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">PhD. Roeb García in his review of the regulatory framework for emerging pollutants in water for Mexico and the world, concludes that “in Mexico and LAM there is not enough regulation regarding emerging pollutants” [12]. Likewise, in literature regarding Oficial Norms in Mexico (NOM), we found five different, related to water treatment and consumption regulations which are resumed in Table I [13]; however, none of them includes maximum limits for EDCs or microplastics (nor microfibers).&nbsp;</span></span><br>
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&nbsp;</p></div><div id="comp-kv00jwrb" class=""><div data-testid="svgRoot-comp-kv00jwrb" class="_3bLYT _2OIRR"><!--?xml version="1.0" encoding="UTF-8"?-->
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        <path d="M116.3 114.4c.7 0 1.3-.2 1.9-.6 12.6-8.2 28.1-9 28.3-9 1.9-.1 3.4-1.7 3.3-3.6-.1-1.9-1.7-3.4-3.6-3.4-.7 0-17.6.8-31.8 10.1-1.6 1.1-2.1 3.2-1 4.8.6 1.2 1.8 1.7 2.9 1.7z" fill="#A67C52" data-color="1"></path>
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</div></div><div id="comp-kuzxky7c" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Speaking of regulations, <span style="font-weight:bold;"><span style="text-decoration:underline"><a href="http://www.dof.gob.mx/nota_detalle.php?codigo=2063863&amp;fecha=22/11/2000" target="_blank" rel="noreferrer noopener">NOM-127-SSA1-1994</a></span> </span>“Environmental health, water for human use and consumption” [14] is the one that comes closest to sanitation in drinking water, taking care of the limits of coliform organisms such as Escherichia coli or physical and organoleptic bodies, as well as chemicals and radioactive characteristics. Therefore, this Oficial Norm became our target to include the microplastics and endocrine disrupting chemicals to its permissible limit characteristics table.&nbsp;</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Complementing this idea, Enresto Robles and Irene Robles, with whom we held an interview, recommended the modification and integration of the particles of interest to the regulations that this same norm supports.</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Developing our biosensor might contribute to the creation of regulatory frameworks and modifications to the Official Standards, more specifically to <span style="text-decoration:underline"><a href="http://www.dof.gob.mx/nota_detalle.php?codigo=2063863&amp;fecha=22/11/2000" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">NOM-127-SSA1-1994,</span> </a></span>regarding the treatment of water for human consumption [15].</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">With the certainty that in our country, it is a step that has been given the opportunity to take. Since the proposals of “Citizen Legislators” regarding Deputy Julieta Macías’ update proposal to the Official Mexican Norms relative to “maximum permissible limits of pollutants and establish limits of contamination by plastic microfibers” was approved to modifications by the secretariat of environment and natural resources. In the mentioned document, she dictates that "we must implement actions that stop the damage to ecosystems and the health of human beings caused by the use of these microfibers" [16], giving the green light and encouraging researchers to develop a detection method with the viable option to achieve this task regarding microfibers. We set this project as a goal.</span></span></p></div><div id="comp-kv0095jl" class="_2UdPt"></div><div id="comp-kuzxofid" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size: 25px;"><span style="font-size:25px;"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">Table I.</span></span></span></h2></div><div id="comp-kuzxovk8" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Mention of the five Mexican Official Norms regarding drinking water and their regulations for human consumption. Making emphasis on the lecture that none of these mentions EDCs or microplastics as regulatory normativity [15].</span></span></p></div><div id="comp-kuzy6yx1" class=""><div data-testid="svgRoot-comp-kuzy6yx1" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 20 160 160" viewBox="20 20 160 160" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
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</div></div><section id="comp-kuz1aplc" class="_3d64y"><div id="bgLayers_comp-kuz1aplc" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1aplc" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1aple" class="_1vNJf"><div id="bgLayers_comp-kuz1aple" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1aple" class="_2GUhU"><wix-image id="img_comp-kuz1aple" class="_1-6YJ _3SQN- _2L00W _1Q5R5 bgImage" data-image-info="{&quot;containerId&quot;:&quot;comp-kuz1aple&quot;,&quot;alignType&quot;:&quot;top&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:2520,&quot;height&quot;:1418,&quot;uri&quot;:&quot;d211b2_382d6f4c486e471083a6cff4579ec0b3~mv2.jpg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-has-bg-scroll-effect="" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_382d6f4c486e471083a6cff4579ec0b3~mv2.jpg/v1/fill/w_1839,h_245,al_t,q_85,usm_0.66_1.00_0.01/d211b2_382d6f4c486e471083a6cff4579ec0b3~mv2.webp"><img src="https://images.pexels.com/photos/5726794/pexels-photo-5726794.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="" style="width: 1471px; height: 196px; object-fit: cover; object-position: 50% 0%;"></wix-image></div></div><div data-mesh-id="comp-kuz1apleinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1apleinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1aplg" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:59px; text-align:justify"><span style="font-weight:bold"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span style="font-size:59px"><span style="color:#FAFAFA">Interviews with specialists</span></span></span></span></p></div></div></div></div></div></section><section id="comp-kuzyg4uv" class="_3d64y"><div id="bgLayers_comp-kuzyg4uv" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzyg4uv" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzyg4wa" class="_1vNJf"><div id="bgLayers_comp-kuzyg4wa" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzyg4wa" class="_2GUhU"></div></div><div data-mesh-id="comp-kuzyg4wainlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzyg4wainlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzyg4wd" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black"><span style="font-style:italic;">Distribuidora Quimica Tauro,&nbsp;</span>specialists in water treatment with Ernesto Robles and Irene Robles</span></span></span></h2></div><div id="comp-kuzyg4wf1" class=""><div data-testid="svgRoot-comp-kuzyg4wf1" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 69 160 62" viewBox="20 69 160 62" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
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         <path d="M166.554 131H33.446C26.094 131 20 124.984 20 117.513V82.487C20 75.113 25.998 69 33.446 69h133.108C173.906 69 180 75.016 180 82.487v35.027c0 7.47-6.094 13.486-13.446 13.486z"></path>
 
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</div></div><div id="comp-kuzyg4wk" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-weight:bold"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF">Why did we do this?&nbsp;</span></span></span></span></p><br>
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</div></div><div id="comp-kv13a7bw" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA">[1] L. Sørensen, A. S. Groven, I. A. Hovsbakken, O. Del Puerto, D. F. Krause, A. Sarno, and A. M. Booth, “UV degradation of natural and synthetic microfibers causes fragmentation and release of polymer degradation products and chemical additives”, Science of The Total Environment, vol. 755, no. 2, February 2021</span></span></span><br>
 
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-weight:bold"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard">​</span></span></span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF">It was carried out in order to know the water sanitation scenarios in Mexican industries, also knowing under what regulations these water treatments are attached and what treatments are currently available for wastewater treatment in Mexico. Simildly, he discussed with specialists the parameters that are considered to be able to establish that the treated water is free of pathogenic microorganism or some other parameter outside it.</span></span></span></p>
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<h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard">​</span></span></span></h2></div><div id="comp-kuzyjm4t" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What did we learn?</span></span></span></h2></div><div id="comp-kuzyk2pv" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size: 17px; text-align: justify;"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We learned about the technical parameters that are measured to consider that a water is ready for consumption either human or as part of a process in the industry, as well as the maximum permissible parameters in water treatment.</span></span></span></p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><br>
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&nbsp;</p>
 
&nbsp;</p>
  
<p class="font_8" style="font-size: 17px; text-align: justify;"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The existing regulations and the new regulations that will enter into force for the discharge of water from different industries such as the textile industry, metalmecanica, drinks and food that are polluting the drainage and drinking water of some rivers in the Mexican region were known. This helped us to direct our project as it was checked with the specialists that none of the existing regulations in Mexico nor in NOM-127 for human consumption take into account EDCs as part of a measurable parameter drinking water because&nbsp; there are currently processes to treat water and remove very small particles such as reverse osmosis, however, these EDCs are not being taken into account within a measurable parameter in the degradation of the bottles used to wire drinking water as once you have the final presentation of the water bottle the parameters that occur after this packaging are not measured. [14]</span></span></span></p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA">[2] A. I. Catarino, R. Thompson, W. Sanderson, and T. B. Henry, “Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues”, Environmental Toxicology and Chemistry, vol. 36, no. 4, pp. 947–951, August 2016</span></span></span></p>
  
<p class="font_8" style="font-size: 17px; text-align: justify;">&nbsp;</p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA"><span class="wixGuard">​</span></span></span></span></p>
  
<p class="font_8" style="font-size: 17px; text-align: justify;"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">On the other hand having the interview allowed us to visualize the scope of our project because we did that the process we are developing is more economically accessible compared to the existing processes in the market for treating water then our process would become more profitable and therefore more attractive for companies to acquire our product and with this we would manage to give solution to the water treatment by obtaining drinking water and safe for them</span></span></span></p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA">[3] M. Godoy. (2020, October 19). Study: Plastic Baby Bottles Shed Microplastics When Heated. Should You Be Worried? [Online]. Available: <span style="text-decoration:underline"><a href="https://www.npr.org/sections/goatsandsoda/2020/10/19/925525183/study-plastic-baby-bottles-shed-microplastics-when-heated-should-you-be-worried" target="_blank" rel="noreferrer noopener">https://www.npr.org/sections/goatsandsoda/2020/10/19/925525183/study-plastic-baby-bottles-shed-microplastics-when-heated-should-you-be-worried</a></span></span></span></span></p>
  
<p class="font_8" style="font-size: 17px; text-align: justify;">&nbsp;</p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA"><span style="text-decoration:underline"><span class="wixGuard">​</span></span></span></span></span></p>
  
<p class="font_8" style="font-size: 17px; text-align: justify;"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Likewise, there was a perspective on how industries should consider treating their water regardless of which area they are covering, as they should stop seeing it as a requirement and more as a contribution to environmental care and population health.</span></span></span></p>
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<p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FAFAFA">[4] INTERNATIONAL MARITIME ORGANIZATION (n.d.). Sources, fate and effects of microplastics in the marine environment: a global assessment. [Online]. Available:<span style="text-decoration:underline"><a href="https://ec.europa.eu/environment/marine/good-environmental-status/descriptor-10/pdf/GESAMP_microplastics%20full%20study.pdf" target="_blank" rel="noreferrer noopener"> https://ec.europa.eu/environment/marine/good-environmental-status/descriptor-10/pdf/GESAMP_microplastics%20full%20study.pdf</a></span></span></span></span></p></div></div></div></div></div></section><section id="comp-kv13a7ak" class="_3d64y"><div id="bgLayers_comp-kv13a7ak" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7ak" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7al2" class="_1vNJf"><div id="bgLayers_comp-kv13a7al2" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7al2" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7al2inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7al2inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7ay" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size: 27px;"><span style="font-size:27px;"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black" id="quinto">Collaboration</span></span></span></h2></div><div id="comp-kv13a7b02" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:23px"><span style="font-family:wfont_08587a_171f7f7b917f46c4971b30f58f09b1e3,wf_171f7f7b917f46c4971b30f58,orig_nunito_light"><span style="font-size:23px"><span style="color:#001489">Monterrey Collaboration</span></span></span></h2></div><div id="comp-kv13a7az" class="_2UdPt"></div><div id="comp-kv19ts7a" class=""><div data-testid="svgRoot-comp-kv19ts7a" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="10.2 22 180.709 131.6" viewBox="10.2 22 180.709 131.6" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
 
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<p class="font_8" style="font-size:17px"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard"></span></span></span></span></p>
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<h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard"></span></span></span></h2></div><div id="comp-kuzysdf0" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What was the impact of<br>
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this activity on our project?</span></span></span></h2></div><div id="comp-kuzysym3" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">It helped us see how our project involving new technologies both biological and non-biological can help companies in many industries in their wastewater treatment as Mexican regulations are increasingly demanding as part of most companies. specifically to the drinking water industry as it has a direct impact on consumer health.</span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify">&nbsp;</p>
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<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The area of opportunity with our project in this sector is of great contribution to have healthy populations and sustainable ecosystems. Especially considering that most companies begin to have a sense of responsibility and respect for the environment and in addition our project could be more profitable to the companies.(To know more about it click <span style="text-decoration:underline;"><a href="https://drive.google.com/file/d/10veexPS0HJuOo-s5puLPe9a-ZpegEV9B/view?usp=drivesdk" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span>)</a></span> &nbsp;</span></span></span></p>
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<h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard">​</span></span></span></h2></div><div id="comp-kv00lklc" class=""><div data-testid="svgRoot-comp-kv00lklc" class="_3bLYT _2OIRR"><svg preserveAspectRatio="xMidYMid meet" data-bbox="34.5 28 130.999 144" viewBox="34.5 28 130.999 144" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="color" role="presentation" aria-hidden="true"><defs><style>#comp-kv00lklc svg [data-color="1"] {fill: #FFC600;}</style></defs>
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</div></div></div></div></div></div></section><section id="comp-kuzyvhcp" class="_3d64y"><div id="bgLayers_comp-kuzyvhcp" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzyvhcp" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzyvheb" class="_1vNJf"><div id="bgLayers_comp-kuzyvheb" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzyvheb" class="_2GUhU"></div></div><div data-mesh-id="comp-kuzyvhebinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzyvhebinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzyvhee" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:35px"><span style="font-size:35px"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span class="color_14">Interviews with Ana Guadalupe Rodríguez Hernández</span></span></span></h2></div><div id="comp-kuzyx3fh" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto;"><span style="font-size:29px"><span style="color:#001489">Ana Guadalupe specialist in nanomaterials with a current project in nanoplastics</span></span></span></h2></div><div id="comp-kuzyvheh" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">Why did we do this?</span></span></p><br>
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</div></div><div id="comp-kv13a7b21" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">To widen our study about the presence of microplastics in bottled water, we collaborated with the iGEM TEC MTY team, designing a protocol to use the Zetasizer device (read more <span style="text-decoration:underline"><a href="https://2021.igem.org/Team:TecCEM/Collaborations" target="_blank" rel="noreferrer noopener">here</a></span>) and asking them to analyze samples of water bottles commonly found in their region. This would allow us to make a comparison between the microplastic particles' presence at different conditions in the bottles they analyzed, and the bottles our team analyzed at the same conditions. Furthermore, as the iGEM TEC MTY team only used plastic bottles, their results could help us to validate the data we obtained regarding the conditions that have a major effect in particle liberation from this material. On the other hand, both teams belong to the same country; thus, our collaborative study could set the path for acknowledging the microplastics problematic in our community and establishing preventive measures to diminish their intake.</span></span></p></div><div id="comp-kv13a7b5" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kv13a7bg" class="_3d64y"><div id="bgLayers_comp-kv13a7bg" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7bg" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7bi1" class="_1vNJf"><div id="bgLayers_comp-kv13a7bi1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7bi1" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7bi1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7bi1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7bk2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The iGEM TEC MTY team applied the following conditions to their samples:</span></span></p>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The engineer is dedicated to the characterization of nanoplastics and also studies the impact of them on health from the water industry and the cosmetics industry and its derivatives. &nbsp;</span></span></p>
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<ul class="font_8" style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light; font-size:17px">
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<li>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">No treatment (normal conditions at which it was purchased)</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Constant sunlight exposure for 12-16 hours, with daily exposure for 2 weeks (14 days)</span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Exposure to 37 ° C for 1 week (7 days)</span></span></p>
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<li>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Constant agitation of 250 RPM for 1 week</span></span></p>
 +
</li>
 +
<li>
 +
<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">1 m impact resistance</span></span></p>
 +
</li>
 +
</ul>
  
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">This is why the interview with the engineer was carried out in order to further enrich our project and to know different points of view as well as to know the real approach of EDCs in human health.</span></span><br>
+
<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">They analyzed bottled water from the brands presented below:</span></span></p>
&nbsp;</p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">What did we learn?</span></span></p>
+
<br>
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Specifically speaking of EDCs and their health damage, Bisphenol A is a mutagenic component that comes from polyethylene degradation and phthalates come from PET degradation.</span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">These EDCs cause interfaces with hormone signaling, produce changes and alterations in human physiology and metabolism, and PET due to its absorption capacity can alter human receptors when it is consumed unintentionally, this can be very dangerous to health because the innate immune system only recognizes these particulates at the nanoplastics level, triggering the immune response and producing a cellular alteration.&nbsp;</span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">At the genetic level there are 4 over-expressed regulators that may be related to various diseases such as cancer. Likewise, these EDCs intervene in the path of calcium, nitrous oxide, and ionic compounds present in the tissues causing the smooth tissue and at the intestinal level to generate involuntary contractions altering the entire regulatory system of our body.</span></span></p></div><div id="comp-kuzyvhej" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-weight:bold"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">What was the impact of this activity on our project?</span></span></span><br>
+
&nbsp;</p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">From the information provided by the interview we enriched more our project and the impact that we would have on it in the field of human health would be important since in Mexico today the reason why they do not exist in the regulations or in the norms these Measurable parameters such as EDCs in water is because there is no knowledge on the subject. This path of research on the subject has recently been opened in Mexico.</span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We as a research team can generate the knowledge and are giving a possible solution to this problem that covers many areas of Mexican industry and various products that have not yet been investigated in depth and of which are unaware of its impact to the cheers. (To learn More about it click <span style="text-decoration:underline;"><a href="https://drive.google.com/file/d/1wd8qQ8N-bZHIRz4DrjEvpjcYgLKYZj1s/view" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span>)</a></span></span></span></p></div></div></div></div></div></section><section id="comp-kuzzgk2c" class="_3d64y"><div id="bgLayers_comp-kuzzgk2c" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzzgk2c" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzzgk3t" class="_1vNJf"><div id="bgLayers_comp-kuzzgk3t" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzzgk3t" class="_2GUhU"><wix-image id="img_comp-kuzzgk3t" class="_1-6YJ _3SQN- _2L00W _1Q5R5 bgImage" data-image-info="{&quot;containerId&quot;:&quot;comp-kuzzgk3t&quot;,&quot;alignType&quot;:&quot;center&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:2255,&quot;height&quot;:1500,&quot;uri&quot;:&quot;d211b2_c18394c181884ea689a55b179c1e0d0b~mv2.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-has-bg-scroll-effect="" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_c18394c181884ea689a55b179c1e0d0b~mv2.jpeg/v1/fill/w_1839,h_213,al_c,q_85,usm_0.66_1.00_0.01/d211b2_c18394c181884ea689a55b179c1e0d0b~mv2.webp"><img src="https://images.pexels.com/photos/802221/pexels-photo-802221.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-802221.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width: 1471px; height: 170px; object-fit: cover; object-position: 50% 50%;"></wix-image></div></div><div data-mesh-id="comp-kuzzgk3tinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzzgk3tinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzzgk3y1" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:45px; text-align:center"><span style="font-size:45px"><span style="font-weight:bold"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span style="color:#FAFAFA">Plastic Consumption Surveys</span></span></span></span></p></div></div></div></div></div></section><section id="comp-kv00qzx3" class="_3d64y"><div id="bgLayers_comp-kv00qzx3" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv00qzx3" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv00qzz4" class="_1vNJf"><div id="bgLayers_comp-kv00qzz4" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv00qzz4" class="_2GUhU"></div></div><div data-mesh-id="comp-kv00qzz4inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv00qzz4inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv00qzz61" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:30px; text-align:justify"><span style="font-size:30px"><span class="color_14"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">Interviews with Ingrid Marión Lopéz Mejía</span></span></span></p>
+
 
+
<p class="font_8" style="font-size:30px; text-align:justify"><span style="color:#45ACFF"><span style="font-size:30px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="wixGuard">​</span></span></span></span></p>
+
<br>
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="color:#45ACFF"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span style="font-size:17px">Interview with Ingrid Lopéz&nbsp; chief of the department of water and Luis Pacheco chief of the department of drinking water and Bertha Muñoz technical director.</span></span></span></p><br>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span class="color_14"><span style="font-size:17px">Why did we do this?</span></span></span></p><br>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Currently, the part of the drinking water regulation in Mexico is not well defined and is why as part of having greater knowledge in the existing regulations for the use and treatment of drinking water, I consider conducting an interview with experts in the subject and with it being able to sustain our project.</span></span></span></p></div><div id="comp-kv00qzze1" class="_2UdPt"></div><div id="comp-kv00qzzc" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv00qzzc" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv00qzzc&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:2100,&quot;height&quot;:1500,&quot;uri&quot;:&quot;d211b2_d1632a126cfc4f8ca52936296b3fdf85~mv2.jpeg&quot;,&quot;name&quot;:&quot;pexels-photo-416528.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_d1632a126cfc4f8ca52936296b3fdf85~mv2.jpeg/v1/fill/w_479,h_343,al_c,q_80,usm_0.66_1.00_0.01/pexels-photo-416528_jpeg_auto%3Dcompress%26cs%3Dtinysrgb%26dpr%3D2%26h%3D750%26w%3D1260.webp"><img src="https://images.pexels.com/photos/416528/pexels-photo-416528.jpeg?auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260" alt="pexels-photo-416528.jpeg_auto=compress&amp;cs=tinysrgb&amp;dpr=2&amp;h=750&amp;w=1260.jpeg" style="width:383px;height:274px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv00st0a" class="_2UdPt"></div><div id="comp-kv00qzz72" class=""><div data-testid="svgRoot-comp-kv00qzz72" class="_3bLYT _2OIRR"><svg preserveAspectRatio="xMidYMid meet" data-bbox="43 41 114 118" viewBox="43 41 114 118" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="color" role="presentation" aria-hidden="true"><defs><style>#comp-kv00qzz72 svg [data-color="1"] {fill: #A0A09F;}</style></defs>
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    <g>
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        <path d="M157 152.999l-22.019-22.195c19.248-20.952 18.781-53.777-1.419-74.14C123.539 46.563 110.213 41 96.037 41c-14.174 0-27.499 5.563-37.522 15.662-20.687 20.857-20.687 54.79 0 75.646 10.021 10.103 23.349 15.667 37.522 15.667 12.031 0 23.445-4.021 32.752-11.411L151.049 159l5.951-6.001zM64.468 126.31c-17.438-17.577-17.438-46.071 0-63.647 8.718-8.786 20.142-13.18 31.569-13.18s22.856 4.393 31.571 13.18c17.436 17.576 17.436 46.07 0 63.647-8.716 8.786-20.145 13.18-31.571 13.18-11.426-.001-22.851-4.394-31.569-13.18z" fill="#2F54DD" data-color="1"></path>
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    </g>
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</svg>
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</div></div><div id="comp-kv00qzz92" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:18px; text-align:justify"><span class="color_14"><span style="font-size:18px"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">What did we learn?</span></span></span></p>
+
<br>
+
<p class="font_8" style="font-size:15px"><span class="wixGuard">​</span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">It was corroborated with the experts on the subject that so far there is no regulation involving EDCs in drinking water and the industries dedicated to this sector are based solely on NOM-127 for drinking water consumption, this does not cover EDCs or microplastics within their regulatory framework.</span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Likewise, it is known that at present around 80% of the population in the State of Mexico has access to drinking water, being that it is a right that every citizen should have this resource as a right to health and a dignified life.&nbsp;</span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The specialists consulted also carry out physicochemical and organoleptic studies based on NOM-127, as regards the organoleptic studies the water should be considered colorless and inholory and which are considered as the first line of study to determine that the water is clean.</span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span style="font-size:17px">What was the impact of this activity on our project?</span></span></span></p><br>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span style="font-size:17px"><span class="wixGuard">​</span></span></span></span></p>
+
 
+
<p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The Mexican regulation applicable to the use and consumption of water does not yet include measurable parameters such as EDCs or microplastics, however, our project may be a precursor that these parameters can be included in this regulatory standard as part of a modification to this one in order to facilitate consideration by the agencies that carry out these analyzes within the analysis of drinking water and thereby contribute to consumer health and a better quality of life as standards are constantly evolving by new technology and advance in knowledge it may be possible to achieve a change in it that includes EDCs in the future. (To learn more about it, click <span style="font-weight:bold"><span style="text-decoration:underline"><a href="https://drive.google.com/file/d/11-6MxObdPm0lz5gZLIRSUESLdqdKdNzX/view" target="_blank" rel="noreferrer noopener">here</a></span></span>)&nbsp;</span></span></span></p></div></div></div></div></div></section><section id="comp-kuzzn5v0" class="_3d64y"><div id="bgLayers_comp-kuzzn5v0" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzzn5v0" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuzzn5wt" class="_1vNJf"><div id="bgLayers_comp-kuzzn5wt" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuzzn5wt" class="_2GUhU"></div></div><div data-mesh-id="comp-kuzzn5wtinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuzzn5wtinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuzzn5wz1" class=""><div data-testid="svgRoot-comp-kuzzn5wz1" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 69 160 62" viewBox="20 69 160 62" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
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        <path d="M166.554 131H33.446C26.094 131 20 124.984 20 117.513V82.487C20 75.113 25.998 69 33.446 69h133.108C173.906 69 180 75.016 180 82.487v35.027c0 7.47-6.094 13.486-13.446 13.486z"></path>
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    </g>
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</svg>
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</div></div><div id="comp-kuzzn5x4" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:18px; text-align:justify"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium"><span style="font-size:18px"><span style="color:#FFFFFF"><span style="font-weight:bold">Why did we do this?&nbsp;</span></span></span></span></p>
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<br><br>
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<p class="font_8" style="font-size:18px; text-align:justify"><span style="font-size:18px"><span style="color:#FFFFFF"><span style="font-weight:bold"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></span></p>
+
  
<p class="font_8" style="font-size:18px; text-align:justify"><span style="font-size:18px"><span style="color:#FFFFFF">This survey had the objective of identifying the plastic production footprint that people who live in the metropolitan area of Mexico have, and more specifically, understanding the exposure that they have to microplastics.&nbsp;</span></span></p>
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<ul class="font_8" style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light; font-size:17px">
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<li>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Brand #1</span></span></p>
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</li>
 +
<li>
 +
<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">H2O BioLeve</span></span></p>
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</li>
 +
<li>
 +
<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Wonu Agua</span></span></p>
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</li>
 +
</ul>
  
<p class="font_8" style="font-size:18px; text-align:justify"><span style="font-size:18px"><span style="color:#FFFFFF">We seek to calculate the microplastic’s footprint people have by analyzing the number and type of plastics that they generate in a week (i.e. plastic bottles, food wrappers, plastic bags, etc.). Once this data was collected, it was analyzed and processed to understand habits related to the exposure to plastic, and how they may affect people’s health.</span></span></p>
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<p class="font_8" style="font-size:17px">&nbsp;</p>
  
<p class="font_8" style="font-size:18px; text-align:justify"><span style="font-size:18px"><span style="color:#FFFFFF">Finally, by doing this we aimed to raise awareness of the impact that the generation of plastics has and the general trends that people have regarding this issue.</span></span></p></div><div id="comp-kuzzn5x51" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What did we learn?</span></span></span></h2></div><br><div id="comp-kuzzn5x63" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Along this research we were able to understand the perception that the Mexico’s City Metropolitan Area population had regarding microplastics, plastic production, and their exposure to all this waste. Plus, through the quantitative analysis performed, we were able to identify how much waste people generated and how this translated into microplastics generated.</span></span></span></p>
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<p class="font_8" style="font-size:17px"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The results for each brand are shown below:</span></span></p></div><div id="comp-kv17qjmb" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv17qjmb" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv17qjmb&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:1062,&quot;uri&quot;:&quot;d211b2_dc6cec0b674d4c69b88ed86d29618b98~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--bonafont.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_dc6cec0b674d4c69b88ed86d29618b98~mv2.png/v1/fill/w_590,h_391,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--bonafont_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/3/3f/T--TecCEM--bonafont.jpeg" alt="1600px-T--TecCEM--bonafont.jpeg.png" style="width:472px;height:313px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv13a7bn1" class="_2UdPt"></div><div id="comp-kv1874s9" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Similar to the trend pointed out for the data obtained by the team, the data shown in the previous five graphs of “Intensity” vs “Size” suggest that, independently from the nature of the treatment or condition to what the bottled water was exposed, there were particles smaller than 2780 nm in diameter (the smallest ones that were detected are of 0.6470 nm) (keep in mind that the bottles were made of plastic). It is worth mentioning that there were two peaks in almost every graph (for almost every tested condition). The only scenario where there was just one peak was the fourth scenario, where the bottle was constantly shaken (250 rpm, for seven days).&nbsp;</span></span></p>
  
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">For instance, thanks to this survey we learned that, even though microplastics are a real threat that can impact negatively the health of people, people in the metropolitan area are not really aware of what this type of waste an one example is that actually are more women with 41% who have a low impact with their carbon footprint, so they have a lower plastic production with 48.7% compared to men who have a high plastic production with 68.6% also those people who have access to drinking water with 41.5% are the ones who consume the most bottles compared to those people who do not have access to drinking water who represent 37.6% of the metropolitan population. Therefore, more men with access to drinking water will produce more plastic waste in the metropolitan area of Mexico.&nbsp;</span></span></span></p>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Additionally, it is interesting to note that the condition number one, which is no treatment at all, “yielded” two peaks, and one of them corresponds to particles of 0.6470 nm and the other one to particles of 2780 diameter. Considering this, and the predicted sizes for the particles detected in the other scenarios, it could be hypothesized that some particles of 2780 nm were reduced in size and lead to the apparition of particles of 171, 265.6, 110.1, 1477 and maybe even particles of 2400 nm. However, with the data at hand it is not possible to determine whether the smaller particles come from the particles that had a diameter of 2780 nm or if they are newly detached particles (recently detached from the inner wall of the bottle).</span></span></p>
  
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">In accordance with the above and with the results obtained from the survey, people over or equal to 30 years of age, whether women or men with a normal lifestyle and with access to drinking water, are the group that is currently producing more waste from bottles of water plastic. However, when evaluating the number of plastic pieces produced per week, it turned out that when considering the average of the comparative factor of the type of gender, it did not have a significant impact in terms of the number of plastic pieces produced by people who have more than 30 years with a normal lifestyle and access to clean water.&nbsp;</span></span></span></p>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Finally, and considering the data obtained from the experiments conducted by iGEM TecCEM team, even though it corresponds to the same brand of bottled water, and although the conditions tested by this team and iGEM TEC MTY were virtually identical, the particle sizes were of different sizes; as the biggest particles detected by iGEM TecCEM (for Brand #1 bottles) were of 405.1 nm in diameter. Nevertheless, more data is needed to statistically determine the difference between these two datasets.</span></span></p></div><div id="comp-kv18m2u1" class="_2UdPt"></div><div id="comp-kv18o7az" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv18o7az" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv18o7az&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:904,&quot;uri&quot;:&quot;d211b2_c3da2a8302c8413bac7fb7a7bff6285c~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--bioleve.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_c3da2a8302c8413bac7fb7a7bff6285c~mv2.png/v1/fill/w_750,h_424,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--bioleve_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/b/ba/T--TecCEM--bioleve.jpeg" alt="1600px-T--TecCEM--bioleve.jpeg.png" style="width:600px;height:339px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv18qehf" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">The five previous graphs correspond to the brand “H2O BioLeve” and the water from the bottles that was analyzed was contained in plastic bottles.&nbsp;<br>
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For three out of five conditions, two groups of particles were identified and, for the other two conditions, just one group of particles was identified. Once again, in the cases where two groups of particles were detected, one group corresponded to particles of a diameter greater that 2000 nm (more specifically, either 2780 or 5560 nm) and the other groups is made up of particles that have a diameter of one order of magnitude smaller that the diameter of the bigger particles.</span></span></p>
  
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">On the other hand, it was observed from the results that currently there are more minors who have knowledge about microplastics with 38.8% of the population that are completely aware of the damage that they cause. Therefore, those people who do not have access to drinking water turn out to be more aware of the damage that microplastics can cause in the long term and which is consistent with the results described above, since those people who have access to drinking water are the oldest. producers of plastic and with less awareness on this issue.&nbsp;</span></span></span></p>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Recalling the data shared by iGEM TEC MTY for Brand #1 bottles, it is interesting that, among these two brands (Brand #1 and H2O BioLeve), BioLeve has the biggest particles of all, regardless of the condition applied to the bottled water. Talking about this maximum value registered, this value is associated with the bottled (H2O BioLeve) water that was analyzed after a 1 meter drop. Keeping in mind the previous relation, it would be useful to have more data (repetitions) to determine if this condition has in fact a statistical significance diameter of the measured particles.&nbsp;</span></span></p></div><div id="comp-kv18ubqx" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv18ubqx" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv18ubqx&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1600,&quot;height&quot;:903,&quot;uri&quot;:&quot;d211b2_e7c49c0dc080445cb6ba067f53993072~mv2.png&quot;,&quot;name&quot;:&quot;1600px-T--TecCEM--wonu.jpeg.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_e7c49c0dc080445cb6ba067f53993072~mv2.png/v1/fill/w_750,h_424,al_c,q_85,usm_0.66_1.00_0.01/1600px-T--TecCEM--wonu_jpeg.webp"><img src="https://static.igem.org/mediawiki/2021/7/73/T--TecCEM--wonu.jpeg" alt="1600px-T--TecCEM--wonu.jpeg.png" style="width:600px;height:339px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv18vgtl" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Regarding the five previous graphs, they are the graphical representation of the size distribution of the particles detected for samples of “Woni Agua” brand of water. The bottle is made of cardboard.</span></span></p>
 
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<h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard"></span></span></span></h2></div><div id="comp-kuzzn5x92" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">A relevant aspect that motivated us to continue with the research of our project was that the trend of people who use bottles to store their water use materials other than plastic, resulting in that this type of people are those under 30 years old representing 60.4% of the mostly female gender and with a lifestyle with a low impact on the carbon footprint, which turns out to be a total of 63.2% of the population that has a low production of plastic bottles, in this sense the parameter of access to water drinking turned out to be non-significant. It also turned out that the type of material that is used as an alternative to plastic is considered reusable by most of the respondents.&nbsp;</span></span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
 
<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Regarding the analysis of different types of brands, it served us as a measure as a reference to the development of our biosensor since the results of the surveys showed that most people prefer the consumption of bonafont because they consider it has better water quality and is a brand that cares about the environment in comparison with the Great Value and “Casa del agua” brands, which were obtained a lower score considering it as low quality water by the respondents, it was also observed that brands such as Fiji, Evian and Rainforest were considered as brands with a high cost and not many of the respondents are aware of these.&nbsp;</span></span></span></p>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">As with the analysis derived from the dataset for Brand #1 shared by iGEM TEC MTY, for this brand of bottled water, four out of five conditions are associated with two populations of particles. And, once again, these two populations differ by one order of magnitude between them (comparing the data from “Peak 1” column with the data from “Peak 2” column). By the way, the condition of a meter drop for this brand of water also led to particles as big as the biggest ones detected for “H2O BioLeve” when the bottle was left in free fall (1 m). These two maxima represent the scenarios where the biggest particles (in diameter) were found, not just for the dataset shared by iGEM TEC MTY, but also for the dataset we obtained (iGEM TecCEM).</span></span></p></div><div id="comp-kv18v1ds" class="_2UdPt"></div><div id="comp-kv18wf1d" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:27px"><span style="font-size:27px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">Overall analysis</span></span></span></h2></div><div id="comp-kv18wf27" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">In conclusion, “Wonu Agua” and “H2O BioLeve” were the brands that, after a drop of 1 meter, theri bottled water had the biggest particles of all, even considering the data obtained by iGEM TecCEM. Nevertheless, it is important to keep in mind that “Wonu Agua”’s bottles are made of a different material than “H2O BioLeve”’s bottles; as the first ones are made of cardboard/paper and the latter ones are made of plastic.&nbsp;<br>
 
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In spite of apparently being related to the biggest particles of all, the one meter impact test did not lead to a “local” maximum particle size for Brand #1 water (just considering the dataset shared by iGEM TEC MTY). Actually, for Brand #1 water yielded the “local” biggest particles just for the scenario where the bottled water was exposed to sunlight. However, if we consider the dataset obtained by iGEM TecCEM, it is also true that the biggest particles for the Brand #1 brand are the ones that were obtained after exposing the bottle to sunlight.&nbsp;</span></span></p></div></div></div></div></div></section><section id="comp-kv17gh3j" class="_3d64y"><div id="bgLayers_comp-kv17gh3j" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv17gh3j" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv17gh58" class="_1vNJf"><div id="bgLayers_comp-kv17gh58" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv17gh58" class="_2GUhU"></div></div><div data-mesh-id="comp-kv17gh58inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv17gh58inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv17gh5a" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black" id="sexto">Portugal Collaboration</span></span></span></h2></div><div id="comp-kv17gh5b1" class=""><div data-testid="svgRoot-comp-kv17gh5b1" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="20 69 160 62" viewBox="20 69 160 62" height="200" width="200" xmlns="http://www.w3.org/2000/svg" data-type="shape" role="presentation" aria-hidden="true">
<p class="font_8" style="font-size:17px; text-align:justify"><br>
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<span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">From the responses of the respondents it was possible to have an even clearer approach about our project since they knew the views, preferences and needs of the population that we sought to give a benefit in the first instance because, while it is true that half of the people surveyed have drinking water in their homes, there is also another 50% of this population that does not have drinking water in their homes. In addition to the fact that from this perspective, the respondents disagree that the water they consume is completely healthy since at least half of them have seen plastic or particles float in their daily drinking water, which is why they have considered using additional technology to have healthier water consumption, this technology includes a filtration system in their homes.</span></span></span></p>
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<h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span style="color:#FFFFFF"><span class="wixGuard">​</span></span></span></h2></div><div id="comp-kuzzn5x81" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:29px"><span style="font-size:29px"><span style="color:#001489"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black">What was the impact of this activity on our project?</span></span></span></h2></div><div id="comp-kuzzz7w5" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span class="color_14"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">This survey was of high added value to our project because it provided us with additional information that we use to evaluate the potential impact of our project on the daily life of Mexicans, in addition to knowing which population group is the one that produces the most plastic waste and because they do it, just as it allowed us to know which people are the ones with the lowest consumption and therefore the lowest production of plastic products. What caught the attention of this was that patterns such as young age and with a low impact lifestyle remained constant in terms of the production of their plastic in the environment without taking so much into consideration the gender to which they belong, this being It is very helpful for us because it allows us to clearly assess the trends to a greater or lesser consumption of plastic products that is related to the development of certain diseases in their long-term consumption. (To learn more about of our completely results regarding to this click <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Results" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span>)</a></span>&nbsp;</span></span></span></p></div><div id="comp-kv001v871" class="_2UdPt"></div></div></div></div></div></section><section id="comp-kv011gvk" class="_3d64y"><div id="bgLayers_comp-kv011gvk" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv011gvk" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv011gxn" class="_1vNJf"><div id="bgLayers_comp-kv011gxn" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv011gxn" class="_2GUhU"></div></div><div data-mesh-id="comp-kv011gxninlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv011gxninlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv01287s" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size: 43px;"><span style="font-size:43px;"><span style="color:#001489;"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold">Meetup expert consultation</span></span></span></h2></div><div id="comp-kv021zii" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv021zii" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv021zii&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1080,&quot;height&quot;:1080,&quot;uri&quot;:&quot;d211b2_2eec0e9d12a246c98eb7df1c68d73992~mv2.jpg&quot;,&quot;name&quot;:&quot;T--TecCEM--Meetup.jpg&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_2eec0e9d12a246c98eb7df1c68d73992~mv2.jpg/v1/fill/w_496,h_496,al_c,q_80,usm_0.66_1.00_0.01/T--TecCEM--Meetup.webp"><img src="https://static.igem.org/mediawiki/2021/e/e4/T--TecCEM--Meetup.jpg" alt="T--TecCEM--Meetup.jpg" style="width:397px;height:397px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv012s7r" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:35px"><span style="color:#45ACFF"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:35px">Why did we do this?</span></span></span></h2></div><div id="comp-kv012875" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">As part of our collaborations, we hosted the LATAM Meetup 2021, where different teams from Mexico, Costa Rica, Brazil, Portugal, and Spain got together through an online gathering to present their projects, share ideas, and receive feedback from our experts panel (read more <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Collaborations" target="_blank" rel="noreferrer noopener"><span style="font-weight:bold;">here</span></a></span>). This was intended to help teams prepare for the Giant Jamboree as well as the iGEM Design League, and to know the teams’ members for possibly establishing collaborations.</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Besides getting to know the projects of our fellow iGEMers, this activity gave us the opportunity of reaching out to different experts, who provided valuable feedback for our project. Specifically, Dr. Yolanda Guadalupe García Huante helped us a lot. She has focused in studying regulatory mechanisms to produce secondary metabolites of Streptomyces, and in the recombinant production of enzymes from thermophilic fungi. Furthermore, she has recently finished a postdoc in the University of Texas at Austin, related to engineering CRISPR-Cas proteins and the expression and characterization of retrons in yeast. Her comments on our ideas about the biosensor and about the production of the laccase enzyme for its usage in the EDCs degradation, contributed to better define our protocols and our strategy of implementation.</span></span></p></div><div id="comp-kv014j91" class="_2UdPt"></div><div id="comp-kv011pu8" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:35px"><span style="color:#45ACFF"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:35px">&nbsp;What did we learn?</span></span></span></h2></div><div id="comp-kv011pv0" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">When we were preparing our presentation for the Meetup, we tried to include all the technical aspects of the project and the lab work we had done by that time. However, we didn’t realize that we hadn't considered several aspects, which came to our notice when Dr. Yolanda García asked some questions and gave us feedback at the end of our presentation. One of the things she told us was that it would be important to add enzymatic assays in our protocols to test the laccase activity and to perform a biochemical characterization of the enzyme and the microorganism from which we obtained it, in order to know the optimal operation conditions. Additionally, we hadn’t defined whether we would extract and immobilize the enzyme or use a complete microorganism for the degradation process, so she told us that we should evaluate the pros and cons of each alternative to choose the one that best suited our idea.</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Another aspect we hadn’t thought about was that, in case our biosensor became 100% functional, who would be our end users (water purification enterprises or people from the community), which she said was very important to determine for the implementation of the project.<br>
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All these observations were very useful and helped us to enhance several parts of our project.</span></span></p></div><div id="comp-kv014j9s" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:35px"><span style="color:#45ACFF"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:35px">What was the impact of this activity on our project?</span></span></span></h2></div><div id="comp-kv014jag" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Following the recommendations of Dr. Yolanda García, we performed laccase induction assays with guaiacol and citrate buffer with colorants to measure the enzyme’s activity as well as several experiments for its the characterization (see the protocols <a href="https://2021.igem.org/Team:TecCEM/Notebook">here</a>); however, we still haven’t decided if we are going to use the purified and immobilized enzyme or the complete microorganism for the degradation of the EDCs. On this matter, it is important to notice that, as the biosensor was the main part of our project, we initially dedicated all our time and effort to its development, leaving the degradation part as a secondary priority, that is why there still many aspects we need to work on regarding this section of the project; nevertheless, we are in the process of doing so and incorporating all the observations and feedback of Dr. Yolanda García to our project.</span></span></p>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
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<span style="font-size:17px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">On the other hand, we think that once our biosensor and the degradation process are fully working, our device could be used by both enterprises and people from the community.</span></span></p></div></div></div></div></div></section><section id="comp-kuz1aplh1" class="_3d64y"><div id="bgLayers_comp-kuz1aplh1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1aplh1" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuz1aplj1" class="_1vNJf"><div id="bgLayers_comp-kuz1aplj1" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuz1aplj1" class="_2GUhU"></div></div><div data-mesh-id="comp-kuz1aplj1inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuz1aplj1inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuz1apll" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:40px"><span style="color:#45ACFF"><span style="font-family:wfont_093ca4_4dcfda9e6d1242458c6bd410b0b78ced,wf_4dcfda9e6d1242458c6bd410b,orig_nunito_regular">References:</span></span></h2></div><div id="comp-kuz1aplq" class="_2UdPt"></div><div id="comp-kuz1aplu" class=""><div data-testid="svgRoot-comp-kuz1aplu" class="_3bLYT _2OIRR"><svg preserveAspectRatio="none" data-bbox="17.3 68.2 165.3 63.6" xmlns="http://www.w3.org/2000/svg" viewBox="17.3 68.2 165.3 63.6" role="presentation" aria-hidden="true">
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         <path d="M166.554 131H33.446C26.094 131 20 124.984 20 117.513V82.487C20 75.113 25.998 69 33.446 69h133.108C173.906 69 180 75.016 180 82.487v35.027c0 7.47-6.094 13.486-13.446 13.486z"></path>
 
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</div></div><div id="comp-kuz1aply2" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:15px"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[1] Rodwan, J. (2021, July). Bottled water 2020: continued upward movement. U.S. and International Developments and Statistics [Online]. Available: <a data-auto-recognition="true" href="https://bottledwater.org/wp-content/uploads/2021/07/2020BWstats_BMC_pub2021BWR.pdf" target="_blank">https://bottledwater.org/wp-content/uploads/2021/07/2020BWstats_BMC_pub2021BWR.pdf</a></span></p>
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</div></div><div id="comp-kv17gh5e2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:17px; text-align:center"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_12"><span style="font-size:17px">We also collaborated with the NOVA Portugal team, sending them the same protocol as we did to the iGEM TEC MTY team (read more <span style="text-decoration:underline;"><a href="https://2021.igem.org/Team:TecCEM/Collaborations" target="_blank" rel="noreferrer noopener">here)</a></span>. Their analysis would help us to identify the differences between the microplastic presence in bottled water from distinct countries and various brands, when subjecting the bottles to the same conditions; however, we are still in the process of interpreting the results that the team sent us, so we can’t make a comparison yet.</span></span></span></h2></div></div></div></div></div></section><section id="comp-kv13a7by" class="_3d64y"><div id="bgLayers_comp-kv13a7by" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7by" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kv13a7c0" class="_1vNJf"><div id="bgLayers_comp-kv13a7c0" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kv13a7c0" class="_2GUhU"></div></div><div data-mesh-id="comp-kv13a7c0inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kv13a7c0inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kv13a7c2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:29px"><span style="color:#FFC600"><span style="font-size:29px"><span style="font-family:wfont_2ce676_938e19a738164a83b7725886d1293a6f,wf_938e19a738164a83b7725886d,orig_nunito_black" id="septimo">Microplastics obtained from sanding bottles experiment.</span></span></span></h2></div><div id="comp-kv13a7c3" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">PET bottles were sanded to emulate the detachment of microplastics from bottles that are normally sold commercially and expose them to daily conditions, such as solar radiation, heat, agitation and impact.</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[2] Alexedo (2021, July). Mexico’s Battle Against Bottled Water [Online]. Available: <a data-auto-recognition="true" href="https://www.alxedo.mx/blog/mexicos-battle-against-bottled-water" target="_blank">https://www.alxedo.mx/blog/mexicos-battle-against-bottled-water</a></span></p>
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<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">We divided the experiment into 3 parts to make an analysis using UV-spectrophotometer scanning and measure them to see if it remains the same value (average of 0.070 Absorbance at 290nm corresponding to microplastics) or with exposicion if there's any emergent bodies and which sizes are them. EDCs must be smaller than the 0.070A.</span></span></span><br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[3] INEGI ( 2017). National household survey. [Online] Available: <a data-auto-recognition="true" href="https://www.inegi.org.mx/programas/enh/2017/" target="_blank">https://www.inegi.org.mx/programas/enh/2017/</a></span></p>
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&nbsp;</p></div><div id="comp-kv194vl9" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv194vl9" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv194vl9&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:1280,&quot;height&quot;:960,&quot;uri&quot;:&quot;d211b2_a1c0f9f3711b40fd954211ad0d8ca8b0~mv2.png&quot;,&quot;name&quot;:&quot;T--TecCEM--microplastic_sanding.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_a1c0f9f3711b40fd954211ad0d8ca8b0~mv2.png/v1/fill/w_531,h_399,al_c,q_85,usm_0.66_1.00_0.01/T--TecCEM--microplastic_sanding.webp"><img src="https://static.igem.org/mediawiki/2021/8/8c/T--TecCEM--microplastic_sanding.png" alt="T--TecCEM--microplastic_sanding.png" style="width:425px;height:319px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv195ktz" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size: 25px;"><span style="font-size:25px;"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold;"><span style="color:#FFC600">A- Samples without any exposure and no Laccase.</span></span></span></h2></div><div id="comp-kv199a82" class="_2UdPt"></div><div id="comp-kv195kuv" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Remains of the sanding were placed in a citrate buffer suspension 1 mg/mL. Were kept in a 50 ml erlenmeyer flask under conditions with no sunlight, no agitation, nor impact.&nbsp;</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[4] UNICEF/WHO (2017). Progress on household drinking water , sanitation and hygiene. [Online] Available: <a data-auto-recognition="true" href="https://data.unicef.org/resources/progress-drinking-water-sanitation-hygiene-2019/" target="_blank">https://data.unicef.org/resources/progress-drinking-water-sanitation-hygiene-2019/</a></span></p>
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<span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_1229ac_dd52f3dbf5c4479998a2f50ec00b3bb8,wf_dd52f3dbf5c4479998a2f50ec,orig_roboto_medium">Result:</span><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"> no other values of interest were observed. Meaning no smaller emerging bodies were present in these samples.&nbsp;</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span></span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[5] Cántaro Azul (2017). Problematics of drinking water in México. [Online] Available: <a data-auto-recognition="true" href="https://www.cantaroazul.org/problematica" target="_blank">https://www.cantaroazul.org/problematica</a></span></p>
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<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="wixGuard">​</span>Pure BPA solution that makes measurements where 290 nm is determined as detection, that is why we do absorbance detection. Meaning at 290 nm there's presence of this type of EDC.<br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[6] Cántaro Azul, (n.d.). Agua Segura en Escuelas [Online]. Available: <a data-auto-recognition="true" href="https://www.cantaroazul.org/agua-segura-en-escuelas" target="_blank">https://www.cantaroazul.org/agua-segura-en-escuelas</a></span></p>
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Image shows the highest peak on the UV spectrophotometer screening samples without sun exposure.</span></span></span><br>
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&nbsp;</p></div><div id="comp-kv1985un" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv1985un" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv1985un&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:548,&quot;height&quot;:324,&quot;uri&quot;:&quot;d211b2_15ebc7408c894d6ba485221f256f21e7~mv2.png&quot;,&quot;name&quot;:&quot;T--TecCEM--_Z_BPA_concentration_at_290_nm_png.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_15ebc7408c894d6ba485221f256f21e7~mv2.png/v1/fill/w_545,h_323,al_c,q_85,usm_0.66_1.00_0.01/T--TecCEM--_Z_BPA_concentration_at_290_nm_png.webp"><img src="https://static.igem.org/mediawiki/2021/e/ec/T--TecCEM--_Z_BPA_concentration_at_290_nm_png.png" alt="T--TecCEM--_Z_BPA_concentration_at_290_nm_png.png" style="width:436px;height:258px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv198qb7" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:25px"><span style="font-size:25px"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="color:#FFC600">B - Samples with microplastics left in direct sunlight without Laccase.</span></span></span></h2></div><div id="comp-kv19cdfp" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv19cdfp" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv19cdfp&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:900,&quot;height&quot;:243,&quot;uri&quot;:&quot;d211b2_b81b06e8fbb74a1cb568865deb34d814~mv2.png&quot;,&quot;name&quot;:&quot;T--TecCEM--_FIG_A-_B_comparación_de_Abs_en_muestras_de_MPS.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_b81b06e8fbb74a1cb568865deb34d814~mv2.png/v1/fill/w_1080,h_292,al_c,lg_1,q_85/T--TecCEM--_FIG_A-_B_comparaci%C3%B3n_de_Abs_en_muestras_de_MPS.webp"><img src="https://static.igem.org/mediawiki/2021/5/5b/T--TecCEM--_FIG_A-_B_comparaci%C3%B3n_de_Abs_en_muestras_de_MPS.png" alt="T--TecCEM--_FIG_A-_B_comparación_de_Abs_en_muestras_de_MPS.png" style="width:900px;height:243px;object-fit:cover;object-position:50% 50%"></wix-image></div></div><div id="comp-kv198qc6" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">Prepared in a buffer solution at a concentration of 1 mg/mL and direct sun light.</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;"><span class="wixGuard">​</span></span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[7] Cántaro Azul. (n.d.). NuestrAgua Franquicia Social [Online]. Available: <a data-auto-recognition="true" href="https://www.cantaroazul.org/franquicia-social" target="_blank">https://www.cantaroazul.org/franquicia-social</a></span></p>
+
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">This is to validate the point of the Zeta sizer where it indicates that with sun exposure, there is a shift of peaks to smaller populations in the absorbance measurements, hypothetically corresponding to EDCs. For further analysis we are going to focus on BPA since we already have the Standard curve.&nbsp;</span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[8] Alexedo (2021, July). Mexico’s Battle Against Bottled Water [Online]. Available: <a data-auto-recognition="true" href="https://www.alxedo.mx/blog/mexicos-battle-against-bottled-water" target="_blank">https://www.alxedo.mx/blog/mexicos-battle-against-bottled-water</a></span></p>
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<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="text-align: justify; font-size: 17px;"><br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[9] E. Diamanti-Kandarakis et al., "Endocrine-Disrupting Chemicals: An Endocrine Society Scientific Statement", Endocrine Reviews, vol. 30, n.º 4, pp. 293–342, junio de 2009. [Online]. Available: <a data-auto-recognition="true" href="https://doi.org/10.1210/er.2009-0002." target="_blank">https://doi.org/10.1210/er.2009-0002.</a></span></p>
+
<span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">The readings exposed to the sun give us some small peaks with an average value of 0.045A (concentration of &nbsp;0 mg/mL according to BPAs std curve)and 0.070A (concentration of &nbsp;0.36 mg/mL according to BPAs std curve) of emerging components.&nbsp;</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
+
<p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;"><span class="wixGuard">​</span></span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[10] E. Ingre-Khans, M. Agerstrand y C. Rudén. "Endocrine disrupting chemicals in the marine environment". Department of Environmental Science and Analytical Chemistry (ACES). [Online]. Available: <a data-auto-recognition="true" href="https://balticeye.org/globalassets/fokusomraden/farliga-amnen/edcs-in-the-marine-environment-report.pdf" target="_blank">https://balticeye.org/globalassets/fokusomraden/farliga-amnen/edcs-in-the-marine-environment-report.pdf</a></span></p>
+
  
<p class="font_8" style="font-size:15px"><br>
+
<p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">In the UV spectrophotometer screening of samples, focusing on 290 nm, in fig A and fig B we can find the absorbance of 0.104A and 0.170A respectively. Showing us that the sun exposure does have an effect on the degradation of microplastics increasing compounds like BPA in this case.&nbsp;</span></span></span></p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[11] I. Vázquez del Mercado, Ed., "El derecho humano al agua potable y saneamiento", ISBN: 978-607-729-084-1, Mexico City, Informe de la Comisión Nacional de los Derechos Humanos, diciembre de 2014. [Online]. Available: <a data-auto-recognition="true" href="http://appweb.cndh.org.mx/DerechoAgua/archivos/contenido/CPEUM/H1.pdf" target="_blank">http://appweb.cndh.org.mx/DerechoAgua/archivos/contenido/CPEUM/H1.pdf</a></span></p>
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<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="text-align: justify; font-size: 17px;">&nbsp;</p>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[12] R. García-Arrazola, «Centro del Agua para América Latina y el Caribe,» Revisión del marco regulatorio de contaminantes emergentes en el agua para México y el mundo., 26 08 2013. Biblioteca UNAM.</span></p>
+
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="text-align: justify; font-size: 17px;"><span style="font-size:17px;"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light;">The increase and appearance of smaller species than the inicial is watched with the only difference in exposure to sunlight, at 290 nm we confirm one it's about BPA.</span></span></span><br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[13]. COFEPRIS, "Water", Mexico, NOM. [Online]. Available: <a data-auto-recognition="true" href="http://transparencia.cofepris.gob.mx/index.php/es/marco-juridico/normas-oficiales-mexicanas/agua" target="_blank">http://transparencia.cofepris.gob.mx/index.php/es/marco-juridico/normas-oficiales-mexicanas/agua</a></span></p>
+
&nbsp;</p></div><div id="comp-kv19dc1h" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:25px"><span style="font-size:25px"><span style="font-family:wfont_2bfd2f_a33be52f2f68425ebd19b40c6bfa42cf,wf_a33be52f2f68425ebd19b40c6,orig_nunito_semibold"><span style="color:#FFC600">Samples with microplastics with Laccase.</span></span></span></h2></div><div id="comp-kv19dc2f" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">In this part, the hypothesis is that Laccase can work on those elements that appeared in the suspension due to the degradation of microplastics.</span></span></span></p>
  
<p class="font_8" style="font-size:15px"><br>
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<p class="font_8" style="font-size:17px; text-align:justify"><br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[14] Salud ambiental. Agua para uso y consumo humano. Límites permisibles de calidad y tratamientos a que debe someterse el agua para su potabilización, NOM-127-SSA1-1994, SEGOB, Mexico, 1994. [Online]. Available: <a data-auto-recognition="true" href="http://www.dof.gob.mx/nota_detalle.php?codigo=2063863&amp;amp;fecha=22/11/2000" target="_blank">http://www.dof.gob.mx/nota_detalle.php?codigo=2063863&amp;amp;fecha=22/11/2000</a></span></p>
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<span style="font-size:17px"><span class="color_14"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">Here we run into the noise that protein makes since Lacase wavelength is at 280 nm.<br>
 
+
At the moment it was seen in absorbance data, that in control of microplastics there is a lower concentration of small components than those exposed to the sun. In other cases where there is no laccase present and there is no noise due to its wavelength the opposite as in this case.</span></span></span></p></div><div id="comp-kv19fjyy" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kv19fjyy" class="_1-6YJ _1Fe8-" data-image-info="{&quot;containerId&quot;:&quot;comp-kv19fjyy&quot;,&quot;displayMode&quot;:&quot;fill&quot;,&quot;imageData&quot;:{&quot;width&quot;:594,&quot;height&quot;:331,&quot;uri&quot;:&quot;d211b2_a959f485f40a4cbe8cbdc718eb057d12~mv2.png&quot;,&quot;name&quot;:&quot;T--TecCEM--Fig_C_Abs_en_muestras_de_MPS_con_Lacasa.png&quot;,&quot;displayMode&quot;:&quot;fill&quot;}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_a959f485f40a4cbe8cbdc718eb057d12~mv2.png/v1/fill/w_596,h_333,al_c,lg_1,q_85/T--TecCEM--Fig_C_Abs_en_muestras_de_MPS_con_Lacasa.webp"><img src="https://static.igem.org/mediawiki/2021/e/ee/T--TecCEM--Fig_C_Abs_en_muestras_de_MPS_con_Lacasa.png" alt="T--TecCEM--Fig_C_Abs_en_muestras_de_MPS_con_Lacasa.png" style="width:477px;height:266px;object-fit:cover;object-position:50% 50%"></wix-image></div></div></div></div></div></div></section><div id="comp-kv13a7c71" class="_2UdPt"></div><div id="comp-kv13a7c9" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:40px"><span style="color:#45ACFF"><span style="font-family:wfont_093ca4_4dcfda9e6d1242458c6bd410b0b78ced,wf_4dcfda9e6d1242458c6bd410b,orig_nunito_regular" id="octavo">Conclusion</span></span></h2></div><div id="comp-kv13a7ca2" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"style="text-align:justify;"><h2 class="font_2" style="font-size:17px; text-align:justify"><span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light"><span class="color_14"><span style="font-size:17px"><span style="font-weight:bold">Small peaks relate, EDCs not only degrade in BPA but a good amount of EDCs. We focus on BPA because we studied it in our BPA degradation reaction by Laccase Trametes versicolor then from sample 1 with an average of 0.070A (concentration of 0.36 mg / mL according to the curve) to sample 2 with an average of 0.045A, with the only difference that sample 2 was exposed to sunlight. This difference and values ​​tell us that new small species were created by the sun, just as the resolution of the Zetasizer shows us the same in all plastic bottles.<br>
<p class="font_8" style="font-size:15px"><br>
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Also in this case, figures A and B confirm the increase of BPA after sun UV exposure. &nbsp;And the decrease of BPA with Laccase depending on the concentration.<br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[15] Límites máximos permisibles de contaminantes para las aguas residuales que se reusen en servicio público., NORMA Oficial Mexicana NOM-00.-ECOL-1997, SEGOB, México, 1997. [Online]. Available: <a data-auto-recognition="true" href="http://www.ordenjuridico.gob.mx/Documentos/Federal/wo69207.pdf" target="_blank">http://www.ordenjuridico.gob.mx/Documentos/Federal/wo69207.pdf</a> .</span></p>
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<br>
 
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This experiment reinforces that when there is no exposure to something there are larger species and when heat or some method is applied, small species appear in the readings. Which, in all plastic bottles appear, either with shaking, heat or impact.<br>
<p class="font_8" style="font-size:15px"><br>
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<br>
<span style="font-family:wfont_12b0c7_d3c3b94e587545a9a5ac52010f952511,wf_d3c3b94e587545a9a5ac52010,orig_roboto_light">[16] J. Macías Rábago. "Gaceta del Senado". Gaceta del Senado. <a data-auto-recognition="true" href="https://www.senado.gob.mx/64/gaceta_del_senado/documento/98022" target="_blank">https://www.senado.gob.mx/64/gaceta_del_senado/documento/98022</a></span></p></div></div></div></div></div></section></div></div></div></div></div></div></div></main>
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This strengthens our hypothesis for the zetasizer assay.<br>
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If more smaller species are found when the plastic bottle is subjected to conditions of daily life such as leaving the bottle in the sun, or in the backpack, or in the car, among many other cases.</span></span></span></span></h2></div><div id="comp-kv13a7cd1" class="_2UdPt"></div></div></div></div></div></div></div></div></main>
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<li id="to1" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">Zeta sizer</li>
<li id="to2" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">Trabajo en laboratorio sobre</li>
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<li id="to2" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">PET bottles</li>
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<li id="to3" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">Glass and aluminium bottles</li>
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<li id="to4" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">Overall analysis</li>
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<li id="to8" class="menu_section" style="font-family: Nunito, Times; font-size: 1.1rem">Conclusion</li>
 
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Latest revision as of 03:10, 22 October 2021

Untitled Document i

ZETASIZER | My Site

ZETASIZER

In this section we weill discuss the fact that the most consumed brand in central Mexico is the one with the highest concentration of microparticles thoughout the experiments we carried out, our analysis and collaborations. So, with the aim of detecting the presence of nano and microparticles in bottled water for determining their intake by consumers, we used the Zetasizer device at our university to carry out an analysis of water samples from bottles of different brands at the conditions presented below:

 

  • Condition A: No treatment (water bottles as purchased)

  • Condition B: 37°C for 7 days

  • Condition C: Sun radiation for 7 days ~ 35-38°C

  • Condition D: Impact test*

  • Condition E: Agitation Test for 7 days at oscillatory agitation (250 RPM)*.

 

*These conditions weren’t applied to the glass and aluminum bottles

 

The studied water bottles were from the following brands:

 

  • Brand #1

  • Brand #2

  • Brand #3

  • Brand #4

  • Brand #5

  • Brand #6

  • Brand #7

  • Brand #8

  • Brand #9

 

To make a comparison between the particles liberated in water from bottles of different materials, some of these bottles were made of plastic and others were made of glass and aluminum.

 

The results and the conditions applied for each bottle are shown in the graphs presented below (the X axis represents the size of the particles in nm and the Y axis represents the intensity of the signal in percentage):

pexels-photo-8217434.jpeg_auto=compress&cs=tinysrgb&dpr=2&h=750&w=1260.jpeg
1599px-T--TecCEM--pp1.jpeg.png

PET bottles
Brand #1

Analysis of Brand #1 bottled water demonstrated the presence of particles at all conditions, even when no treatment was applied (condition A). Also, a change in particle dimensions and amount occurred when the bottle was subjected to heat (condition B); the same thing happened for the impact test (Condition D). With sun radiation (condition C) and agitation (condition E), two groups of particles of different sizes appeared, with a distinct distribution for each condition. This showed us that the conditions that had a major effect on the particles release were the solar radiation and agitation. 

Brand #2

For water samples of Brand #2, large particles were detected in condition A; however, for conditions B and C there was presence of particles of smaller sizes, which indicated degradation. In condition D, two groups of particles appeared, but the larger group didn’t have a significant change in dimension respecting the particles from condition A. For condition E, two groups of particles were present as well, but with different dimensions and distribution than those from condition D. This revealed that heat,  radiation and agitation affected most the particle liberation.

1600px-T--TecCEM--epura.jpeg.png

Brand #3

In the water samples of Brand #3, two groups of particles were present in water for conditions A and C; nevertheless, they were different in distribution and amount, showing a change between the two conditions. The impact test performed for condition D only caused the appearance of one group of particles. For condition E, three groups of particles were detected, all of different amounts and sizes. This demonstrated that the particles’ distribution and amount changed when the bottle was subjected to treatment. For condition B, the device indicated “error”; thus, no measurements could be made. 

1599px-T--TecCEM--zoe.jpeg.png
1600px-T--TecCEM--great_value.jpeg.png

Brand #4

Analysis of Brand #4 bottled water showed presence of particles for every condition. For condition B, the particles were of a considerably smaller size than those from condition A, which implied degradation. Also, two groups of particles of different amounts and dimensions appeared for condition C, as well as for condition E. The particles detected in condition D were larger in size than most of the particles from the other conditions. This revealed a greater effect of sun radiation and agitation for the particle liberation.

Brand #5

No particles were detected in Brand #5 water samples for conditions A and D, which indicated a higher quality of the bottle; however, when heat was applied (Condition B), a group of particles appeared in water; the same thing happened when the bottle was subjected to agitation (condition E). The sun radiation exposure (Condition C) caused the liberation of even more particles, presenting three different groups of them; thus, being the major condition for particle generation.

1600px-T--TecCEM--fiji.jpeg.png

Glass and aluminium bottles

Brand #6

Brand #6 water samples featured particles in all three conditions. Particularly, for condition A two groups of particles were detected and just one for conditions B and C. This was unexpected to us, since water is contained in a glass bottle; however, we believe that the plastic cap might be responsible for the particles’ release.

1600px-T--TecCEM--casa_del_agua.jpeg.png

Brand #7

The Brand #7 of water is presented in an aluminium container, the company stating themselves that the container is plastic free. Nonetheless, every metallic container needs a thin layer of protective coat, similar to soda cans. A polymer plastic lining is added to these cans to avoid the soda and aluminum reacting with each other. This may be the reason why this brand of water presents the same kind of nanoparticles throughout the tests.

1600px-T--TecCEM--rain.jpeg.png

Brand #8

Brand #8 of water showed no particles throughout the three tests made. The principal reason this brand may not show any particles according to our study is because the water is contained in a glass bottle, not a plastic one. Demonstrating another great advantage of preferring glass over plastic, apart from is greater simplicity to be recycled.

1600px-T--TecCEM--voss.jpeg.png

Brand #9

Brand #9 of water, just as Brand #8, showed no particles throughout the three tests made. The principal reason this brand may not show any particles according to our study is because the water is contained in a glass bottle, not a plastic one. Demonstrating another great advantage of preferring glass over plastic, apart from is greater simplicity to be recycled.

1600px-T--TecCEM--evian.jpeg.png

Overall analysis

Presence of particles was observed in all PET water bottles at most conditions. In every case, when subjected to heat or heat with solar radiation, a change in dimensions and distribution of the particles occurred. Specifically, a population of smaller particles appeared, which is related to degradation. Also, in general for all brands, no significant changes were observed in either quantity, distribution, or particle size for the impact test. Regarding the agitation test, more than one group of particles was detected for most cases, presenting particles of larger dimensions than those from the other conditions.

On the other hand, there was no presence of particles for two of the glass bottles (Brand #8 and Brand #9) at any condition. We hypothesize that particles detected in the remaining glass bottle (Brand #6) were due to the plastic bottle cap. Also, the presence of particles in the aluminum bottle water sample could be caused by some of the materials used in the bottle manufacturing. 


These results could be explained due to the effects of UV light, high temperatures and agitation on plastic. Particularly, we have found in literature that UV exposure can be an important source of plastic degradation, acting through autocatalytic thermal oxidation, and leading to an increase in the amount of microplastics and microfibers [1]. Furthermore, it has been shown that “plastic polymers have viscoelastic properties that can be altered by temperature…” [2]; in fact, hot water might exacerbate degradation, contributing to the release of microplastics [3]. Additionally, fragmentation of plastic is likely to occur because of agitational and mechanical forces, which might produce cracks in the material, especially if the bottle has been previously exposed to sunlight, becoming weak and brittle [4].


All those findings will allow us to estimate the approximate intake of EDCs considering the amount of microplastics present in each sample, and determine which factors (temperature, sun exposure, brand, etc.) favor the liberation of these substances in bottled water.
 

References:

[1] L. Sørensen, A. S. Groven, I. A. Hovsbakken, O. Del Puerto, D. F. Krause, A. Sarno, and A. M. Booth, “UV degradation of natural and synthetic microfibers causes fragmentation and release of polymer degradation products and chemical additives”, Science of The Total Environment, vol. 755, no. 2, February 2021
 

[2] A. I. Catarino, R. Thompson, W. Sanderson, and T. B. Henry, “Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues”, Environmental Toxicology and Chemistry, vol. 36, no. 4, pp. 947–951, August 2016

[3] M. Godoy. (2020, October 19). Study: Plastic Baby Bottles Shed Microplastics When Heated. Should You Be Worried? [Online]. Available: https://www.npr.org/sections/goatsandsoda/2020/10/19/925525183/study-plastic-baby-bottles-shed-microplastics-when-heated-should-you-be-worried

[4] INTERNATIONAL MARITIME ORGANIZATION (n.d.). Sources, fate and effects of microplastics in the marine environment: a global assessment. [Online]. Available: https://ec.europa.eu/environment/marine/good-environmental-status/descriptor-10/pdf/GESAMP_microplastics%20full%20study.pdf

Collaboration

Monterrey Collaboration

To widen our study about the presence of microplastics in bottled water, we collaborated with the iGEM TEC MTY team, designing a protocol to use the Zetasizer device (read more here) and asking them to analyze samples of water bottles commonly found in their region. This would allow us to make a comparison between the microplastic particles' presence at different conditions in the bottles they analyzed, and the bottles our team analyzed at the same conditions. Furthermore, as the iGEM TEC MTY team only used plastic bottles, their results could help us to validate the data we obtained regarding the conditions that have a major effect in particle liberation from this material. On the other hand, both teams belong to the same country; thus, our collaborative study could set the path for acknowledging the microplastics problematic in our community and establishing preventive measures to diminish their intake.

The iGEM TEC MTY team applied the following conditions to their samples:

  • No treatment (normal conditions at which it was purchased)

  • Constant sunlight exposure for 12-16 hours, with daily exposure for 2 weeks (14 days)

  • Exposure to 37 ° C for 1 week (7 days)

  • Constant agitation of 250 RPM for 1 week

  • 1 m impact resistance

They analyzed bottled water from the brands presented below:

  • Brand #1

  • H2O BioLeve

  • Wonu Agua

 

The results for each brand are shown below:

1600px-T--TecCEM--bonafont.jpeg.png

Similar to the trend pointed out for the data obtained by the team, the data shown in the previous five graphs of “Intensity” vs “Size” suggest that, independently from the nature of the treatment or condition to what the bottled water was exposed, there were particles smaller than 2780 nm in diameter (the smallest ones that were detected are of 0.6470 nm) (keep in mind that the bottles were made of plastic). It is worth mentioning that there were two peaks in almost every graph (for almost every tested condition). The only scenario where there was just one peak was the fourth scenario, where the bottle was constantly shaken (250 rpm, for seven days). 


Additionally, it is interesting to note that the condition number one, which is no treatment at all, “yielded” two peaks, and one of them corresponds to particles of 0.6470 nm and the other one to particles of 2780 diameter. Considering this, and the predicted sizes for the particles detected in the other scenarios, it could be hypothesized that some particles of 2780 nm were reduced in size and lead to the apparition of particles of 171, 265.6, 110.1, 1477 and maybe even particles of 2400 nm. However, with the data at hand it is not possible to determine whether the smaller particles come from the particles that had a diameter of 2780 nm or if they are newly detached particles (recently detached from the inner wall of the bottle).


Finally, and considering the data obtained from the experiments conducted by iGEM TecCEM team, even though it corresponds to the same brand of bottled water, and although the conditions tested by this team and iGEM TEC MTY were virtually identical, the particle sizes were of different sizes; as the biggest particles detected by iGEM TecCEM (for Brand #1 bottles) were of 405.1 nm in diameter. Nevertheless, more data is needed to statistically determine the difference between these two datasets.

1600px-T--TecCEM--bioleve.jpeg.png

The five previous graphs correspond to the brand “H2O BioLeve” and the water from the bottles that was analyzed was contained in plastic bottles. 
For three out of five conditions, two groups of particles were identified and, for the other two conditions, just one group of particles was identified. Once again, in the cases where two groups of particles were detected, one group corresponded to particles of a diameter greater that 2000 nm (more specifically, either 2780 or 5560 nm) and the other groups is made up of particles that have a diameter of one order of magnitude smaller that the diameter of the bigger particles.


Recalling the data shared by iGEM TEC MTY for Brand #1 bottles, it is interesting that, among these two brands (Brand #1 and H2O BioLeve), BioLeve has the biggest particles of all, regardless of the condition applied to the bottled water. Talking about this maximum value registered, this value is associated with the bottled (H2O BioLeve) water that was analyzed after a 1 meter drop. Keeping in mind the previous relation, it would be useful to have more data (repetitions) to determine if this condition has in fact a statistical significance diameter of the measured particles. 

1600px-T--TecCEM--wonu.jpeg.png

Regarding the five previous graphs, they are the graphical representation of the size distribution of the particles detected for samples of “Woni Agua” brand of water. The bottle is made of cardboard.


As with the analysis derived from the dataset for Brand #1 shared by iGEM TEC MTY, for this brand of bottled water, four out of five conditions are associated with two populations of particles. And, once again, these two populations differ by one order of magnitude between them (comparing the data from “Peak 1” column with the data from “Peak 2” column). By the way, the condition of a meter drop for this brand of water also led to particles as big as the biggest ones detected for “H2O BioLeve” when the bottle was left in free fall (1 m). These two maxima represent the scenarios where the biggest particles (in diameter) were found, not just for the dataset shared by iGEM TEC MTY, but also for the dataset we obtained (iGEM TecCEM).

Overall analysis

In conclusion, “Wonu Agua” and “H2O BioLeve” were the brands that, after a drop of 1 meter, theri bottled water had the biggest particles of all, even considering the data obtained by iGEM TecCEM. Nevertheless, it is important to keep in mind that “Wonu Agua”’s bottles are made of a different material than “H2O BioLeve”’s bottles; as the first ones are made of cardboard/paper and the latter ones are made of plastic. 
In spite of apparently being related to the biggest particles of all, the one meter impact test did not lead to a “local” maximum particle size for Brand #1 water (just considering the dataset shared by iGEM TEC MTY). Actually, for Brand #1 water yielded the “local” biggest particles just for the scenario where the bottled water was exposed to sunlight. However, if we consider the dataset obtained by iGEM TecCEM, it is also true that the biggest particles for the Brand #1 brand are the ones that were obtained after exposing the bottle to sunlight. 

Portugal Collaboration

We also collaborated with the NOVA Portugal team, sending them the same protocol as we did to the iGEM TEC MTY team (read more here). Their analysis would help us to identify the differences between the microplastic presence in bottled water from distinct countries and various brands, when subjecting the bottles to the same conditions; however, we are still in the process of interpreting the results that the team sent us, so we can’t make a comparison yet.

Microplastics obtained from sanding bottles experiment.

PET bottles were sanded to emulate the detachment of microplastics from bottles that are normally sold commercially and expose them to daily conditions, such as solar radiation, heat, agitation and impact.

We divided the experiment into 3 parts to make an analysis using UV-spectrophotometer scanning and measure them to see if it remains the same value (average of 0.070 Absorbance at 290nm corresponding to microplastics) or with exposicion if there's any emergent bodies and which sizes are them. EDCs must be smaller than the 0.070A.
 

T--TecCEM--microplastic_sanding.png

A- Samples without any exposure and no Laccase.

Remains of the sanding were placed in a citrate buffer suspension 1 mg/mL. Were kept in a 50 ml erlenmeyer flask under conditions with no sunlight, no agitation, nor impact. 


Result: no other values of interest were observed. Meaning no smaller emerging bodies were present in these samples. 

Pure BPA solution that makes measurements where 290 nm is determined as detection, that is why we do absorbance detection. Meaning at 290 nm there's presence of this type of EDC.
Image shows the highest peak on the UV spectrophotometer screening samples without sun exposure.

 

T--TecCEM--_Z_BPA_concentration_at_290_nm_png.png

B - Samples with microplastics left in direct sunlight without Laccase.

T--TecCEM--_FIG_A-_B_comparación_de_Abs_en_muestras_de_MPS.png

Prepared in a buffer solution at a concentration of 1 mg/mL and direct sun light.

This is to validate the point of the Zeta sizer where it indicates that with sun exposure, there is a shift of peaks to smaller populations in the absorbance measurements, hypothetically corresponding to EDCs. For further analysis we are going to focus on BPA since we already have the Standard curve. 


The readings exposed to the sun give us some small peaks with an average value of 0.045A (concentration of  0 mg/mL according to BPAs std curve)and 0.070A (concentration of  0.36 mg/mL according to BPAs std curve) of emerging components. 

In the UV spectrophotometer screening of samples, focusing on 290 nm, in fig A and fig B we can find the absorbance of 0.104A and 0.170A respectively. Showing us that the sun exposure does have an effect on the degradation of microplastics increasing compounds like BPA in this case. 

 

The increase and appearance of smaller species than the inicial is watched with the only difference in exposure to sunlight, at 290 nm we confirm one it's about BPA.
 

Samples with microplastics with Laccase.

In this part, the hypothesis is that Laccase can work on those elements that appeared in the suspension due to the degradation of microplastics.


Here we run into the noise that protein makes since Lacase wavelength is at 280 nm.
At the moment it was seen in absorbance data, that in control of microplastics there is a lower concentration of small components than those exposed to the sun. In other cases where there is no laccase present and there is no noise due to its wavelength the opposite as in this case.

T--TecCEM--Fig_C_Abs_en_muestras_de_MPS_con_Lacasa.png

Conclusion

Small peaks relate, EDCs not only degrade in BPA but a good amount of EDCs. We focus on BPA because we studied it in our BPA degradation reaction by Laccase Trametes versicolor then from sample 1 with an average of 0.070A (concentration of 0.36 mg / mL according to the curve) to sample 2 with an average of 0.045A, with the only difference that sample 2 was exposed to sunlight. This difference and values ​​tell us that new small species were created by the sun, just as the resolution of the Zetasizer shows us the same in all plastic bottles.
Also in this case, figures A and B confirm the increase of BPA after sun UV exposure.  And the decrease of BPA with Laccase depending on the concentration.

This experiment reinforces that when there is no exposure to something there are larger species and when heat or some method is applied, small species appear in the readings. Which, in all plastic bottles appear, either with shaking, heat or impact.

This strengthens our hypothesis for the zetasizer assay.
If more smaller species are found when the plastic bottle is subjected to conditions of daily life such as leaving the bottle in the sun, or in the backpack, or in the car, among many other cases.