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<div class="section-contents-mid large-img"> | <div class="section-contents-mid large-img"> | ||
− | <p> | + | <p>In the existing part BBa_K2982005, we added Scanning Electron Microscope (SEM) photos of PET films after |
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being digested by S245I PETase mutant. The usage of SEM can accurately visualize how serious the PET film is | being digested by S245I PETase mutant. The usage of SEM can accurately visualize how serious the PET film is | ||
digested by the enzyme. Besides, we added HPLC profile of products released from PET film digested with | digested by the enzyme. Besides, we added HPLC profile of products released from PET film digested with | ||
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<div class="single-image-with-desc"> | <div class="single-image-with-desc"> | ||
− | <center><img src="https://static.igem.org/mediawiki/2021/ | + | <center><img src="https://static.igem.org/mediawiki/2021/c/ce/T--HK_GTC--Contribution--a.png" alt=""></center> |
<p>Figure 2a. HPLC spectrum of TPA standard. </p> | <p>Figure 2a. HPLC spectrum of TPA standard. </p> | ||
</div> | </div> | ||
<div class="single-image-with-desc"> | <div class="single-image-with-desc"> | ||
− | <center><img src="https://static.igem.org/mediawiki/2021/ | + | <center><img src="https://static.igem.org/mediawiki/2021/5/58/T--HK_GTC--2b.png" alt=""></center> |
<p> Figure 2b. HPLC spectrum of MHET standard.</p> | <p> Figure 2b. HPLC spectrum of MHET standard.</p> | ||
</div> | </div> | ||
<div class="single-image-with-desc"> | <div class="single-image-with-desc"> | ||
− | <center><img src="https://static.igem.org/mediawiki/2021/ | + | <center><img src="https://static.igem.org/mediawiki/2021/9/99/T--HK_GTC--2d.png" alt=""></center> |
<p> Figure 2c. HPLC spectrum of products released from the PET film digested with S245I PETase.</p> | <p> Figure 2c. HPLC spectrum of products released from the PET film digested with S245I PETase.</p> | ||
</div> | </div> | ||
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hydrolysis as considerable amounts of intermediate product, MHET which showed a peak at 5.07 minutes were | hydrolysis as considerable amounts of intermediate product, MHET which showed a peak at 5.07 minutes were | ||
detected (Figure 2c). <br> | detected (Figure 2c). <br> | ||
− | Therefore, we can conclude that S245I PETase | + | Therefore, we can conclude that S245I PETase exhibits hydrolytic activity for PET depolymerization. |
</p> | </p> | ||
Latest revision as of 14:22, 20 October 2021