Difference between revisions of "Team:HK GTC/Parts"

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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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        <h1>Parts</h1>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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        <h1> <font color="black"> Parts </font> </h1>
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<p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
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        <groupparts>iGEM21 HK_GTC</groupparts>
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This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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      <h2 style"width:"100%; text-align:center;">Overview</h2>
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      <p style="width:"100%; text-align:center;">We created 4 basic parts and 2 composite parts.</p>
  
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      <h2>BBa_K3787001 </h2>
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      <p>
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        PETase(WT)-MHETase chimera<br>
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        Composite part (Coding)<br>
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        Length: 2715 bp<br>
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        The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in <i>E.
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        coli</i>.<br>
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        It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
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        serine glycine linker.<br>
  
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      </p>
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      <h2> BBa_K3787002 </h2>
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      <p> PETase(WT)-MHETase chimera <br>
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        Composite part (Coding) <br>
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        Length: 2715 bp <br>
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        The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in <i>E.
 +
        coli</i>.<br>
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        It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
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        serine glycine linker. </p>
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  </div>
  
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      <h2>BBa_K3787003  </h2>
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      <p> I.s. MHETase <br>
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        Basic part (Coding) <br>
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        Length: 1809 bp<br>
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        The coding sequence for MHETase, codon optimized for the gene expression in <i>E. coli</i>. <br>
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        The sequence comes from the bacteria <i>Ideonella sakaiensis</i>. It is used in enzyme cocktail experiments. </p>
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<h3>Adding parts to the Registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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      <h2> BBa_K3787004 </h2>
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      <p>12 amino acid serine glycine linker<br>
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        Basic part (Coding)<br>
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        Length: 36 bp<br>
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        The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form
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        chimeric proteins.
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        <br>
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      </p>
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  </div>
  
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<p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
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      <h2> BBa_K3787008 </h2>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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      <p>
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ADD PARTS
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        MHETase R914 Reverse primer<br>
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</a>
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        Basic part (Primer)<br>
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        Length: 20 bp<br>
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        The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.<br>
  
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      <h2> BBa_K3787009 </h2>
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<h3>Inspiration: Basic Parts</h3>
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        MHETase F416 Forward primer<br>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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        Basic part (Primer)<br>
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        Length: 20 bp<br>
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<p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
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        The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.<br>
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<ul>
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      </p>
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<li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
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<h3>Inspiration: Composite Parts</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at the following examples for Composite Parts on the Registry:</p>
 
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<ul>
 
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<li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
 
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<li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
 
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<li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
 
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<li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
 
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<li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li>
 
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<li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
 
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<h3>Part Table </h3>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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<groupparts>iGEM20 HK_GTC</groupparts>
 
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        <h1>GT COLLEGE iGEM2021</h1>
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        <img width="30%" src="https://static.igem.org/mediawiki/2021/7/78/T--HK_GTC--logo.png" alt="">
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      </div>
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      <div>
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        <h1>Follow Us!</h1>
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        <a href="https://www.instagram.com/gtigemteam/">
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            <i class="icon fab fa-instagram fa-2x"></i>
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        </a>
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      </div>
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      <div>
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        <h1>Contact:</h1>
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        <p>igemteam.gt@gmail.com</p>
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Latest revision as of 10:22, 20 October 2021

HK_GTC 2021 Homepage

Parts

Parts 

       <groupparts>iGEM21 HK_GTC</groupparts>
expand_less

Overview

We created 4 basic parts and 2 composite parts.

BBa_K3787001

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787002

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787003

I.s. MHETase
Basic part (Coding)
Length: 1809 bp
The coding sequence for MHETase, codon optimized for the gene expression in E. coli.
The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.

BBa_K3787004

12 amino acid serine glycine linker
Basic part (Coding)
Length: 36 bp
The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.

BBa_K3787008

MHETase R914 Reverse primer
Basic part (Primer)
Length: 20 bp
The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.

BBa_K3787009

MHETase F416 Forward primer
Basic part (Primer)
Length: 20 bp
The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com