Difference between revisions of "Team:HK GTC/Parts"

 
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<groupparts>iGEM21 HK_GTC</groupparts>
 
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      <div class="content-wrapper parts center">
 
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<h1> <font color="black"> Parts </font> </h1>
 
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        <groupparts>iGEM21 HK_GTC</groupparts>
 
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        </div>
 
  
 
       <div class="section-heading">
 
       <div class="section-heading">
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       </div>
 
       </div>
  
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       <div class="intro">
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</html>
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         <h2>Overview</h2>
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       <div class="content-wrapper parts center">
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        <p>We created 4 basic parts and 2 composite parts.</p>
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         <h1> <font color="black"> Parts </font> </h1>
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       </div>
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        <groupparts>iGEM21 HK_GTC</groupparts>
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        <html>
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         <div class="eachpart">
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         <h2> BBa_K3787001 </h2>
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  <span class="material-icons to-top"><a href="#the-top">expand_less</a></span>
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         <p> Wild Type PETase <br>
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The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants. <br></p>
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  <div class="section-wrapper">
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        </div>
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      <h2 style"width:"100%; text-align:center;">Overview</h2>
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      <p style="width:"100%; text-align:center;">We created 4 basic parts and 2 composite parts.</p>
 +
 
 +
       <h2>BBa_K3787001 </h2>
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      <p>
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         PETase(WT)-MHETase chimera<br>
 +
         Composite part (Coding)<br>
 +
         Length: 2715 bp<br>
 +
        The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in <i>E.
 +
        coli</i>.<br>
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        It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
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        serine glycine linker.<br>
 +
 
 +
      </p>
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  </div>
  
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        <div class="eachpart">
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  <div class="eachpart">
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        <h2> BBa_K3787002 </h2>
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      <h2> BBa_K3787002 </h2>
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        <p> PETase(WT)-MHETase chimera <br>
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      <p> PETase(WT)-MHETase chimera <br>
 
         Composite part (Coding) <br>
 
         Composite part (Coding) <br>
 
         Length: 2715 bp <br>
 
         Length: 2715 bp <br>
−
         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.<br>
+
         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in <i>E.
−
         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker. </p>
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        coli</i>.<br>
−
        </div>
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         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
 +
        serine glycine linker. </p>
 +
  </div>
  
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        <div class="eachpart">
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    <div class="eachpart">
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        <h2> BBa_K3787003 </h2>
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      <h2>BBa_K3787003 </h2>
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        <p> I.s. MHETase <br>
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      <p> I.s. MHETase <br>
−
Basic part (Coding) <br>
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        Basic part (Coding) <br>
−
Length: 1809 bp <br>
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        Length: 1809 bp<br>
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The coding sequence for MHETase, codon optimized for the gene expression in E. coli. <br>
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        The coding sequence for MHETase, codon optimized for the gene expression in <i>E. coli</i>. <br>
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The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.  
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        The sequence comes from the bacteria <i>Ideonella sakaiensis</i>. It is used in enzyme cocktail experiments. </p>
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</p>
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  </div>
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        </div>
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<div class="eachpart">
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  <div class="eachpart">
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        <h2> BBa_K3787004 </h2>
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      <h2> BBa_K3787004 </h2>
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        <p> 12 amino acid serine glycine linker <br>
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      <p>12 amino acid serine glycine linker<br>
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Basic part (Coding)
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        Basic part (Coding)<br>
−
Length: 36 bp <br>
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        Length: 36 bp<br>
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The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.
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        The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form
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</p>
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        chimeric proteins.
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        </div>
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        <br>
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      </p>
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  </div>
  
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        <div class="eachpart">
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  <div class="eachpart">
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        <h2> BBa_K3787008 </h2>
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      <h2> BBa_K3787008 </h2>
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        <p> MHETase R914 Reverse primer <br>
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      <p>
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Basic part (Primer) <br>
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        MHETase R914 Reverse primer<br>
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Length: 20 bp <br>
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        Basic part (Primer)<br>
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The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.  
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        Length: 20 bp<br>
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</p>
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        The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.<br>
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        </div>
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        <div class="eachpart">
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      </p>
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        <h2> BBa_K3787009 </h2>
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        <p> MHETase F416 Forward primer <br>
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  </div>
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Basic part (Primer) <br>
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Length: 20 bp <br>
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  <div class="eachpart">
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The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.
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      <h2> BBa_K3787009 </h2>
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</p>
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      <p>
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         </div>
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        MHETase F416 Forward primer<br>
 +
        Basic part (Primer)<br>
 +
        Length: 20 bp<br>
 +
        The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.<br>
 +
      </p>
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  </div>
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</div>
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  <div class="clear"></div>
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  <footer>
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      <div>
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         <h1>GT COLLEGE iGEM2021</h1>
 +
        <img width="30%" src="https://static.igem.org/mediawiki/2021/7/78/T--HK_GTC--logo.png" alt="">
 +
      </div>
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      <div>
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        <h1>Follow Us!</h1>
 +
        <a href="https://www.instagram.com/gtigemteam/">
 +
            <i class="icon fab fa-instagram fa-2x"></i>
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        </a>
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      </div>
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      <div>
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        <h1>Contact:</h1>
 +
        <p>igemteam.gt@gmail.com</p>
 +
      </div>
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  </footer>
  
 
       </body>
 
       </body>
 
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Latest revision as of 10:22, 20 October 2021

HK_GTC 2021 Homepage

Parts

Parts 

       <groupparts>iGEM21 HK_GTC</groupparts>
expand_less

Overview

We created 4 basic parts and 2 composite parts.

BBa_K3787001

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787002

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787003

I.s. MHETase
Basic part (Coding)
Length: 1809 bp
The coding sequence for MHETase, codon optimized for the gene expression in E. coli.
The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.

BBa_K3787004

12 amino acid serine glycine linker
Basic part (Coding)
Length: 36 bp
The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.

BBa_K3787008

MHETase R914 Reverse primer
Basic part (Primer)
Length: 20 bp
The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.

BBa_K3787009

MHETase F416 Forward primer
Basic part (Primer)
Length: 20 bp
The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com