Difference between revisions of "Team:HK GTC/Safety"

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        <h1>Safety</h1>
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2021.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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        <h1>Safety</h1>
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            <li><a href="#design">Safe project design</a></li>
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            <li><a href="#organ">Safe organisms</a></li>
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            <li><a href="#lab">Safe lab</a></li>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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            <h2 id="design">Safe project design</h2>
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            <p>Our goal of the project is to alleviate and solve the global plastic pollution problem by increasing the PET degradation rate using our dual-enzyme system. Our two enzymes, PETase and MHETase, existing in the forms of chimeric protein or enzyme cocktail would digest PET plastic into its constituting monomers, which can be foreseen as a solution to the global plastic pollution crisis in the future. </p>
  
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            <h2 id="organ">Safe organisms</h2>
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<h3>Safe Project Design</h3>
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            <p>Our team selects non-pathogenic classis for usage. They are safe strains of <i>Escherichia coli</i> called DH5α and C41(DE3) to transform recombinant plasmids - pET21b (+). It carries the protein-coding sequences to produce the protein chimeras. Both of the <i>E.coli</i> strains that we choose have no harm on humans nor the natural environment. As PETase is toxic to <i>E.coli</i>,  using the strain C41(DE3) allows the expression of toxic proteins, so that our protein chimeras can be expressed. <br>
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The chimeric proteins are then extracted and purified for use. Thus no genetically engineered bacteria are released into the environment.
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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            <h2 id="lab">Safe lab</h2>
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            <p>All organisms involved in our experiments pose no serious risks even if escaped from the lab. No hazardous chemicals are used in our experiment. We utilized antiseptic techniques and used Bunsen burner to maintain sterility of the workplace and experimental apparatuses. Our project does not involve pathogenic organisms and animals. Our lab is of low risk and it has safety systems in place, for example eyewash bottle and fire extinguisher. The waste that is generated throughout our project will be disposed of in the biohazard bin. <br>
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<li>Choosing a non-pathogenic chassis</li>
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Our team's PI is an experienced biology teacher and is fully capable of overseeing student members and maintaining lab safety. Also, all student members in our team will only do experiments in the lab under the supervision of our team's PI and a qualified lab technician. Our team members have also received formal safety and security training.
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h3>Safe Lab Work</h3>
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          <h1>GT COLLEGE iGEM2021</h1>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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          <img width="30%" src="https://static.igem.org/mediawiki/2021/7/78/T--HK_GTC--logo.png" alt="">
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          <h1>Follow Us!</h1>
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          <a href="https://www.instagram.com/gtigemteam/">
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          <h1>Contact:</h1>
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          <p>igemteam.gt@gmail.com</p>
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Latest revision as of 15:14, 19 October 2021

HK_GTC 2021 Homepage

Safety

Safety

Safe project design

Our goal of the project is to alleviate and solve the global plastic pollution problem by increasing the PET degradation rate using our dual-enzyme system. Our two enzymes, PETase and MHETase, existing in the forms of chimeric protein or enzyme cocktail would digest PET plastic into its constituting monomers, which can be foreseen as a solution to the global plastic pollution crisis in the future.

Safe organisms

Our team selects non-pathogenic classis for usage. They are safe strains of Escherichia coli called DH5α and C41(DE3) to transform recombinant plasmids - pET21b (+). It carries the protein-coding sequences to produce the protein chimeras. Both of the E.coli strains that we choose have no harm on humans nor the natural environment. As PETase is toxic to E.coli, using the strain C41(DE3) allows the expression of toxic proteins, so that our protein chimeras can be expressed.
The chimeric proteins are then extracted and purified for use. Thus no genetically engineered bacteria are released into the environment.

Safe lab

All organisms involved in our experiments pose no serious risks even if escaped from the lab. No hazardous chemicals are used in our experiment. We utilized antiseptic techniques and used Bunsen burner to maintain sterility of the workplace and experimental apparatuses. Our project does not involve pathogenic organisms and animals. Our lab is of low risk and it has safety systems in place, for example eyewash bottle and fire extinguisher. The waste that is generated throughout our project will be disposed of in the biohazard bin.
Our team's PI is an experienced biology teacher and is fully capable of overseeing student members and maintaining lab safety. Also, all student members in our team will only do experiments in the lab under the supervision of our team's PI and a qualified lab technician. Our team members have also received formal safety and security training.

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com