Difference between revisions of "Team:IISER-Tirupati India/Sustainable"

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                                 <li><a class="dropdown-item" href="https://2021.igem.org/Team:IISER-Tirupati_India/Description">DESCRIPTION</a></li>
 
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                        <h2>Introduction</h2>
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                     <div class="col-md-4 col-lg-3 p-2 d-none d-md-block" style="background-color: #FFBD59 !important; color: #8D1063;">
                        <p>Natural systems are highly complex to understand as well as to experiment with. To make predictions of the outcomes,
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                        <div class="card" style=" max-width: 15.5rem; border: none !important;background-color:#FFBD59;" id="index">
                            we use the available information and the knowledge of physics, mathematics, chemistry, and computer science to build a theoretical model. This page deals with the models built on the different aspects of our project namely :</p>
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                             <div class="card-body">
                            <ol>
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                                 <h3 class="card-title text-center mb-3">INDEX</h3>
                                <li>GMO delivery</li>
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                                <section id="Index1">
                                <li>GMO growth and colonisation</li>
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                                     <ul>
                                <li>Protease production</li>
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                                         <li><a class="index_link" href="#1">#3 Good Health and Well Being</a></li>
                                <li>Diffusion and ovum Hardening</li>
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                                        <li><a class="index_link" href="#2">#4 Quality Education</a></li>
                                <li>Kill switch for reversibility</li>
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                                        <li><a class="index_link" href="#3">#5 Gender Equality</a></li>
                                <li>Kill switch for Biosafety</li>
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                                        <li><a class="index_link" href="#4">#14 Life Below Water</a></li>  
                                </ol>
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                                        <li><a class="index_link" href="#5">#17 Partenerships for Goals</a></li>  
                            <h2>Delivery & Colonisation</h2><br>
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                                     </ul>                        
                            <h3>INTRODUCTION: THE JOURNEY OF GMO BEGINS</h3><br>
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                                 </section>
                            <h4>PART A: Delivery</h4><br>
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                            </div>
                            <h5>OVERVIEW</h5>
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                          </div>
                            <p>Delivery is an essential part of our project as it determines the audience we reach. We tried to develop multiple
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                                ways to deliver GMO in a user-friendly way with minimum invasion. Minimum invasion means it should not colonize
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                                the whole reproductive tract or reach the ovaries. The device should ensure bacterial colonization in the ampulla
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                                of the fallopian tube.
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                                One of the constraints that we faced is the high viscosity of the oviductal fluid.</p><P>
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                                After calculating the time taken for delivery in each method, talking to a couple of IVF experts, and getting their
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                                inputs into it,we thought hysteroscopic techniques would be the best for our purpose. This method gives us an advantage
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                                by delivering the bacteria directly into the ampulla region.</p>
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                            <h5>DEVICE DESIGNING (How to deliver?)</h5>
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                            <p>GMO will be delivered directly to the ampulla by a process called Hysteroscopy. It’s done with medical assistance and requires
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                                constant X-Ray monitoring to ensure the catheter is inserted without any issues. The reason is that the Ostia and the Fallopian
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                                tube are dynamic structures and that there is no chemical or significant physical difference between the ampulla and the other
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                                components of the Fallopian tube.</p>
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                            <p>A solution of GMO of a specific concentration is prepared. Then the catheter is inserted under medical guidance up to the ampulla.
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                                Then an injector is projected with narrow long incisions on both sides of it, which ensures that the GMO forms a ring along the
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                                circumference of the tube. Ovum is now
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                                360° surrounded by the GMO. </p>
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                            <p>The GMO Lactobacillus Acidophilus has pili which expectantly forms hydrogen bonds at the tube circumference with mucin found in mucus.
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                                The GMO is now adhered to the walls and multiplies once it adjusts to the introduced environment (lag phase) </p>
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                            <h5>FUTURE ASPECTS:</h5>
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                            <p>Since we aim to reach a larger population, the hysteroscopic technique, we believe, is too expensive.
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                                We also considered using hydrogels for the delivery of bacteria at a pH-specific region.</p><P>
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                                Why is this system compatible with the delivery of bacteria in the ampulla of the Fallopian tube region?</P><P>
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                                We found out that the pH in the oviductal Ampulla region is close to 8.0.
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                                That’s why we considered using hydrogels for bacterial delivery, which swells up at a pH range of 7.8 to 8.0. </P><P>
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                                Amongst the stimuli-responsive hydrogels, pH-sensitive hydrogels are the most studied hydrogels. The rate at
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                                which hydrogels respond depends upon their size, shape, cross-linking density, number of ionic groups, and composition,
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                                which can be tailored by varying these factors. The response rate increases with increasing pore size and number of ionic
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                                groups and decreasing their size and cross-linking density.</P><P>
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                                For a delivery in a basic medium, we planned to use anionic hydrogels, such as carboxymethyl chitosan, which swell at higher
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                                pH (basic medium) due to ionization of the acidic groups. As a result, the ionized negatively charged pendant groups on the
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                                polymer chains cause repulsion leading to swelling. This property of hydrogels can be exploited for GMO delivery at pH 7.4
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                                in the ampulla of the fallopian tube. </p>
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                            <h5>CELL ENCAPSULATION (What to deliver?)</h5>
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                            <p>Our goal is to design a live-bacteria entrapment system. More than just encapsulating bacteria, we want to entirely prevent
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                                their escape from the bead body into the surroundings.</p>
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                            <p>The Oviductal Fluid is slightly alkaline (ph 7.4 to 7.7), so we need our encapsulating membrane to dissolve away at this pH.
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                                Potential candidates for hydrogel materials include chitosan, guar gum, and xanthan. Chitosan forms Hydrogen bonds with the
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                                mucin protein in the mucus allowing for the anchoring at the walls and preventing the hydrogels from getting washed away.</p>
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                            <h4>PART B: Colonization</h4><br>
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                            <h5>OVERVIEW (why is it necessary to study growth and colonization)</h5>
+
                            <p>In biosynthesis, growth kinetics is a crucial study to be conducted. The growth of a cell comprises both the size and the number.
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                                These growths are affected by external factors such as temperature, the chemical composition of the growth nutrient, etc., and by
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                                different physiological factors such as growth factor proteins[1][2]. The cells in a particular environment extract the nutrients
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                                from the growth media and produce biomolecules, which humans utilize for different purposes. This phenomenon has applications, from
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                                producing delicious Italian wine to getting antibiotics which saves millions of lives every year. We will be using this simple
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                                formula to reach the goals of our project. To have a qualitative understanding of the protein production by the Genetically Modified
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                                anism(GMO), we need to have a theoretical approach. We need to understand how we can calculate the growth of GMOs [3]. This, in turn,
+
                                will help us figure out protein production. This detailed study of growth kinetics will help us calculate the initial inoculation
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                                required for the production of a protein that we need.</p>
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                            <p>For a better understanding, we will be considering two cases. The first comprises the study of the growth of Bacillus Subtilis in
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                                normal petri dish conditions. This is the known environment and we can have this condition easily in computer simulation and/or lab
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                                and reconfirm our model. The second case of study is the continuous culture model where we study the growth of Lactobacillus
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                                Acidophilus in the fallopian tube, which is our target region.  </p>
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                            <h6>GROWTH MODELS</h6><br>
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                            <p>PETRI DISH MODEL (Bacillus Subtilis, curve fitting)</p><br>
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                            <p>CONTINUOUS CULTURE MODEL (lactobacillus acidophilus + fallopian tube environment)</p><br>
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                            <h5>INOCULUM CALCULATIONS (connection with 2nd module)</h5><br>
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                            <h2>Protease production</h2><br>
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                            <h3>OVERVIEW ( overall picture) </h3>
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                            <p>Well known for extracellular protease production[1], Bacillus subtilis is our model organism for the proof of concept experiments. Moreover,
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                                it is a gram-positive bacteria which even if not too close but is closer than e coli (gram-negative) to our proposed bacteria lactobacillus
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                                Acidophilus. This module deals with the whole mechanism of action ideally the GMO must follow for contraception to be attained. To know how
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                            this module developed to what it is, please refer to the engineering success (hyperlink).</p>
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                            <p>Let's create the flow, you must know by now that we plan to produce the protease known as ovastacin,
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                                an indigenous protease[2] to the human ovum. It is known to cause Zona pellucida hardening naturally
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                                used by the ovum to prevent polyspermy[3]. To see the mechanism of zona pellucida hardening click here
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                                (goes to project overview where it is explained).</p>
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                            <h5> 1] Total amount of ovastacin required: </h5>
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                            <p>[We assume one active ovastacin cleaves one ZP2 glycoprotein present in the Zona pellucida matrix. To get the number of molecules of ovastacin needed, we calculated the number of ZP2 glycoproteins present on the surface of the ovum. For this, we reached out to studies that looked at the thickness of the ovum with and without the zona pellucida layer to find its overall thickness and the radius of the ovum[4]. </p>
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                            <br>
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                            <h6>Constants and calculations:</h6><br>
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                                        <tr>
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                                          <th>Parameter (need alternative) </th>
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                                          <th>Value</th>
+
                                          <th>References</th>
+
                                        </tr>
+
                                     </thead>
+
                                    <tbody>
+
                                         <tr>
+
                                          <td>Zona pellucida thickness</td>
+
                                          <td>18.9 μm</td>
+
                                          <td>Does zona pellucida thickness influence the fertilization rate? E. Bertrand1 , M.Van Den Bergh and Y.Englert</td>
+
                                        </tr>
+
                                        <tr>
+
                                          <td>Oocyte diameter</td>
+
                                          <td>123.5 μm</td>
+
                                          <td>Does zona pellucida thickness influence the fertilization rate? E. Bertrand1 , M.Van Den Bergh and Y.Englert</td>
+
                                        </tr>
+
                                        <tr>
+
                                          <td>Size of ZP2</td>
+
                                          <td>90–110 kDa</td>
+
                                          <td>Characterization of human zona pellucida glycoproteins A R Bauskin 1, D R Franken, U Eberspaecher, P Donner DOI: 10.1093/molehr/5.6.534</td>
+
                                        </tr>
+
                                        <tr>
+
                                          <td>Diameter of ZP2r</td>
+
                                          <td>0.0092 μm</td>
+
                                          <td>Zetasizer Nano Sensitivity Calculator (Classic)</td>
+
                                        </tr>
+
                                    </tbody>
+
                                </table>
+
                            </div>                           
+
                            <p>Assuming cuboid with all spheres of size same as ZP2 to
+
                            get volume of one unit = 3.1 e-6 μm3 </p>
+
                            <p>Volume occupied by whole ZP matrix = 12.4 e5 μm3 </p>
+
                            <p>Calculated the number of ZP2 glycoproteins present in the ZP matrix = 3.92 x 1011 molecules</p>
+
                            <p>Assuming 1 ovastacin cleaves 1 ZP2</p>
+
                            <p>No. of ovastacin = No. of ZP2 = 12.4 e5 / 3.1 e-6 = 3.92 x 1011  molecules ]</p>
+
                            <p>In order to cause complete hardening __ number of molecules are required to reach the ovum. The next question that arises is how much is produced and how much of produced ovastacin will reach the ovum. So we have two major things left to look at: </p>
+
                            <h5>2] Production and transport of ovastacin by GMO</h5>  
+
                            <p>The production of ovastacin is required for three days pre and post ovulation, please go to “Genetic Circuits” to learn details regarding the genetic circuit. </p> 
+
                            <h4>GENETIC CIRCUIT</h4>
+
                            <p>Progesterone repressible system</p>
+
                            <h4>JOURNEY OF OVASTACIN</h4>
+
                            <p>From the papers, we know that the ovum is propelled by the ciliary motion away from the uterus and that means the ovum is in contact with the wall. Thesize ampulla region of the Fallopian tube (2.5 mm ≤ radius ≤ 5 mm ) is much larger than the radius of the ovum (61.7μm) A molecule under the influence of Brownian motion move according to the equation</p>
+
                            <ul>
+
                                <li>< r <sup>2</sup> >= 4Dt (for 2-D)</li>
+
                                <li>< r <sup>2</sup> >= 6Dt (for 3-D)</li>
+
                                <p>Where,</p>
+
                                <li>< r <sup>2</sup>  > → mean squared (radial) distance travelled by the molecule</li>
+
                                <p>using,</p>
+
                                <ol>
+
                                    <li>k<sub>B</sub> = 1.38064852 ×10<sup>23</sup> m<sup>2</sup> kg<sup>-2</sup> s<sup>-1</sup> K  [Boltzmann constant]</li>
+
                                    <li>η = 0.799 Pa·s [Viscosity of Oviductal Fluid]</li>
+
                                    <li>T = 35.5 + 273.15 = 308.65 K [Temperature of Oviduct]</li>
+
                                    <li>r = 22kDa = 2.43 nm [Radius of ovastacin molecule]</li>
+
                                    <li>a = 61.7 μm [Radius of the ovum]</li>
+
                                    <li>s = 5 mm [Radius of the ampulla region of the oviduct]</li>
+
                                    <li>N<sub>0</sub> = 3.92 ×10<sup>11</sup>  molecules = 0.000650946529392 nanomoles [Number of ovastacin to react with all ZP2]</li>
+
                                     <p>
+
                                        For, Ovastacin D=1.1644194699 ×10<sup>-13</sup>  m<sup>2</sup> s<sup>-1</sup>
+
                                        </p>
+
                                 </ol>
+
                                <li>t → Time elapsed</li>
+
               
+
                            </ul>
+
 
                     </div>
 
                     </div>
                </div>
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                <div class="card d-none d-md-block" style="max-width: 18rem;width: 25%; border: none !important;" id="index">
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                    <div class="col-md-8 p-5">
                    <div class="card-body">
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                        <p><strong><em>&ldquo;Sustainable development is the pathway to the future we want for all. It offers a framework to generate economic growth, achieve social justice, exercise environmental stewardship and strengthen governance.&rdquo;</em></strong></p>
                      <h5 class="card-title">INDEX</h5>
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                        <ul>
                      <h6 class="card-subtitle mb-2 text-muted">Card subtitle</h6>
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                        <li><strong><em><strong><em>Ban Ki-Moon</em></strong></em></strong></li>
                      <p class="card-text">Some quick example text to build on the card title and make up the bulk of the card's content.</p>
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                        </ul>
                      <a href="#" class="card-link">Card link</a>
+
                        <p>Incorporating sustainability into innovation is the most fundamental yet most challenging aspect when developing solutions. As young scientists, concerned about the future of our planet we wanted to develop a solution that contributes to sustainable development goals. Thus, when designing OviCloak we ensured that sustainability forms the core of our project.</p>
                      <a href="#" class="card-link">Another link</a>
+
                        <p>Through our project, we targeted to achieve 5 SDGs:</p>
                      <ul>
+
                        <p><strong>Goal #3: Good Health and Wellbeing</strong></p>
                          <li><a href="#waste1">waste 1</a></li>
+
                        <p><strong>Goal #4: Quality Education</strong></p>
                          <li><a href="#waste2">waste 2</a></li>
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                        <p><strong>Goal #5: Gender Equality</strong></p>
                          <li><a href="#waste3">waste 3</a></li>
+
                        <p><strong>Goal #14: Life below water</strong></p>
                          <li><a href="#waste4">waste 4</a></li>
+
                        <p><strong>Goal #17: Partnership for goals</strong></p>
                          <li><a href="#waste1">waste 1</a></li>
+
                        <figure class="col-12 mt-5">
                          <li><a href="#waste2">waste 2</a></li>
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                            <img src="./assets/yeh-01.png" alt="Trulli" style="width:100%">
                          <li><a href="#waste3">waste 3</a></li>
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                        </figure>
                          <li><a href="#waste4">waste 4</a></li>
+
                        <p id="1" class="pt-5 pb-3"></p>
                          <li><a href="#waste1">waste 1</a></li>
+
                        <h2>#3 Good Health and well being</h2>
                          <li><a href="#waste2">waste 2</a></li>
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                        <h3 style="background-color: #FFBD59;color:#8D1063 ;" class="p-3">3.7 By 2030, ensure universal access to sexual and reproductive health-care services, including for family planning, information and education, and the integration of reproductive health into national strategies and programmes put in box</h3>
                          <li><a href="#waste3">waste 3</a></li>
+
                        <p>Ensuring universal access to sexual and reproductive health care services can contribute to significant health benefits. Ovicloak, being a contraceptive, can <strong>prevent pregnancy-related health risks in women</strong> and<strong> reduces the risk of infant and maternal mortality</strong>, by reducing the number of births and<strong> assists in child spacing</strong>.</p>
                          <li><a href="#waste4">waste 4</a></li>
+
                        <p>Despite the health and non-health benefits conferred by usage of contraception, the modern contraceptive prevalence worldwide increased by only 2.1 percentage points from 55% to 57.1% between 2000 and 2009. One of the major reasons for the gradual increase in the usage of modern contraceptives is the <strong>fear of side-effects</strong> which deter women from using contraceptives leading to unwanted pregnancies negatively impacting their physical and mental health.</p>
                      </ul>
+
                        <p>Our project, Ovicloak is designed to be a <strong>long term reversible contraceptive with minimal side effects</strong>. In addition, the <strong>reversibility</strong> of the contraceptive allows an individual to <strong>regulate and control their fertility</strong>. These attributes of Ovicloak can <strong>encourage women who do not want to conceive to use contraceptive decreasing the risk of unwanted pregnancies</strong>.</p>
 +
                        <p>The<strong> knowledge about sexual and reproductive health</strong> plays a pivotal role in the accessibility of its health services. Thus, our team contributed to enhancing sexual and reproductive health-care services though two strategies&nbsp;</p>
 +
                        <ol>
 +
                        <li>Collecting data regarding the status of awareness on Reproductive Health and Rights&nbsp;</li>
 +
                        </ol>
 +
                        <ul>
 +
                        <li><strong>Reproductive Health and Rights Survey</strong>: The survey was conducted with the intention of understanding about the extent of awareness on Reproductive Health and Rights and identifying the major sources of sexuality education in India. The survey included participation of 633 participants. (See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>)</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong><strong>In depth Interview</strong>: We conducted an in depth Interview with 8 participants to learn about the general opinion of people on existing contraceptives and their perspective on the need for new contraceptives. This allowed us to obtain qualitative data and understand the respondent&rsquo;s perspective, feelings and experience. (See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>)</strong></li>
 +
                        </ul>
 +
                        <ol start="2">
 +
                        <li>Conducting Sexual and Reproductive Health awareness sessions</li>
 +
                        </ol>
 +
                        <ul>
 +
                        <li><strong>Shush the Hush</strong>: The webinar series was conducted to facilitate discussion on Reproductive Health and Rights, and saw 550+ registrations in total. Due to the conservative nature of Indian society topics surrounding reproductive and sexual health are not widely discussed leading to misconceptions.The session was focussed on debunking myths and misconceptions associated with reproductive and sexual health and educating the public on contraceptive options and safe sexual practices.The webinar conducted received a positive feedback from the participants for the informative session. (See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>).</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong><strong>Ask about it</strong>: The Instagram live series was launched with an intention to promote discussion on Menstrual Health, STI and UTI in the public. The session allowed us to engage with the public actively and break the stigma surrounding these topics. The live series received over 560+ views in total. (See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>)</strong></li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>That skipped chapter</strong>; An Instagram Reel series,through which we communicated with the audience about Sexually transmitted Infection (STI), the importance of consent when engaging in sexual activity, representation of sex in media and porn and the myth surrounding the association of hymen with virginity.</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong><strong>The birds and bees: An Art Exhibition</strong>: We put out an art exhibition on the theme of World Contraception Day &ldquo;Let every pregnancy be a planned one&rdquo; and got artists from all over the country and the world to present their artwork. We received entries in the form of traditional<p id="2"></p> art and paintings which were posted on our social media pages. (See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>)</strong></li>
 +
                        </ul>
 +
                        <p><br /><br /></p>
 +
                        <h2>#4 Quality education&nbsp;</h2>
 +
                        <h3 style="background-color: #FFBD59;color:#8D1063 ;" class="p-3">4.5 By 2030, eliminate gender disparities in education and ensure equal access to all levels of education and vocational training for the vulnerable, including persons with disabilities, indigenous peoples, and children in vulnerable situations</h3>
 +
                        <p>Our project OviCloak is based on providing a synthetic biology approach to contraception. We undertook this UN sustainable development goal as we planned to <strong>educate the people around us on the different aspects of Synthetic Biology.</strong>&nbsp;</p>
 +
                        <p>Synthetic biology has the potential to eradicate the rampant lack of awareness of Biological processes and the scientific method and society at large. The solutions provided by synthetic biology resonate with people and their problems and thus, synthetic biology can cater to the educational needs of people from all backgrounds and become an <strong>effective tool to eliminate gender disparities, provide vocational training equally at all levels and uplift the children in vulnerable situations.</strong>&nbsp;</p>
 +
                        <p>We realised the goal through the following activities:</p>
 +
                        <ul>
 +
                        <li><strong>Gene Gala</strong>: The 5-day Mini Summer School was a special effort by team IISER-Tirupati_India in collaboration with team IISER Kolkata to <strong>end gender disparity in the field of STEM. </strong>Gene Gala saw the constant participation of about 45-50 girl students from high schools under the Directorate of Education, GNCT Delhi. The girl students were able to grasp the concepts of synthetic biology well and had a positive learning experience with our teams. See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>.&nbsp;</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>Translation of Science</strong>: An interactive zine, regarding microbiome was created and translated to a local language Hindi.This exposed students to a new arena of science in a fun mode.It was also accessible to students studying science in schools with the Hindi medium of instruction. See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>.&nbsp;</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>Sign Language</strong>: To increase access of synthetic biology to people who are hard of hearing, we translated our promo video in Sign language, using STEM signs to explain the processes. It helped open up a dialogue between science and the deaf community. See See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a><a href="https://2021.igem.org/Team:IISER-Tirupati_India/Inclusivity">.&nbsp;</a></li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>LAB EYES</strong>: To increase synthetic biology laboratory access to visually impaired individuals, we collaborated with two software engineering graduates from India to design and implement an assistive technology software. This software allows the identification of chemicals and consumables without resorting to touching, smelling, or tasting them.See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>&nbsp;</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong><strong>Agastya: </strong>Our team conducted a virtual synthetic biology workshop session with students from the Navodaya schools through the Agastya foundation. The pandemic has been hard on the<p id="3"></p> student community, depriving such students of excellence the right exposure to the scientific method. Our workshop introduced them to the basics of the scientific method, the tools, and techniques of synthetic biology and ended with a holistic discussion on the process of biological contraception and introduction to our project.See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>.</strong></li>
 +
                        </ul>
 +
                        <h2>#5: Gender Equality</h2>
 +
                        <p>UNICEF have found that Girls are less likely than boys to achieve high proficiency levels in STEM and at upper secondary, fewer girls choose STEM majors.&nbsp;</p>
 +
                        <p>As undergraduates pursuing science and researching in synthetic biology : a more advanced field of science, we tried to achieve<strong> Gender Equality in STEM</strong> through following collaborations with other iGEM teams.</p>
 +
                        <ul>
 +
                        <li><strong>Gene Gala : </strong>A 5-day Mini Summer School in collaboration with iGEM Team of IISER Kolkata, for 45-50 girl students from high schools under the Directorate of Education, GNCT Delhi.The girl students were able to grasp the concepts of synthetic biology,</li>
 +
                        </ul>
 +
                        <p>See<a href="https://2021.igem.org/Team:IISER-Tirupati_India/Education"> Education</a>.&nbsp;</p>
 +
                        <ul>
 +
                        <li><strong>Femme club</strong>: A club initiative of teams Moscow City and Team LMSU, where female members of iGEM teams come together and discuss the <strong>various challenges faced by women in the field of STEM</strong>. This helped us act collectively to overcome the barriers in future.</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>Rosalind Chronicles</strong>: a collaborative initiative by the team of iGEM ULaval iGEM Concordia-Montreal and iGEM Patra, which documented inspiring woman scientist and female team members of each team.This helped us reflect upon our own team and realise <strong>the invaluable role of women in STEM.</strong></li>
 +
                        </ul>
 +
                        <h3 style="background-color: #FFBD59;color:#8D1063 ;" class="p-3">5.6 Ensure universal access to sexual and reproductive health and reproductive rights as agreed in accordance with the Programme of Action of the International Conference on Population and Development and the Beijing Platform for Action and the outcome documents of their review conferences</h3>
 +
                        <p>UNFPA says: <strong>&ldquo;Family planning is central to gender equality and women&rsquo;s empowerment</strong>, and it is a key factor in reducing poverty.&rdquo; They found that when women and couples are empowered to plan their family, women are better able to complete their education; women&rsquo;s autonomy within their households is increased; and their earning power is improved.Thus leading to empowerment of women. OviCloak, being <strong>a long term reversible contraceptive with least to side effects</strong>, was aimed to <strong>ensure gender equality and empower women</strong> by allowing women to regulate their fertility.</p>
 +
                        <p>The UN's World Contraceptive Day (WCD) coalition agrees that<strong> addressing gender inequality </strong>is one of the four areas that needs development globally to avoid/reduce unplanned pregnancy<strong>. </strong>This dependence between family planning and gender equality was striking.</p>
 +
                        <p>Through meetings with stakeholders like <strong>Ms.Kathy Walking</strong> and<strong> Ms.Nalini Jameela</strong>, we understood that to empower girls and young women with the knowledge they need to take control of their sexual and reproductive health, which we did through above mentioned activities like <strong>Shush the Hush, Ask about it, That skipped chapter, and The birds and bees.</strong></p>
 +
                        <p id="4">In addition to achieving equality among binary genders.We also, took a step to address the inequality in the minority genders by addressing the contraceptive needs of <strong>transmasculine spectrum. </strong>This was a result of meeting with our stakeholders Dr.Frances Grimstad and NGO-Sex Education.India.Hence, we believe this<strong> indeed is a novel step towards achieving this SDG</strong>.</p>
 +
 
 +
                        <h2>#14: Life below water</h2>
 +
                        <h3 style="background-color: #FFBD59;color:#8D1063 ;" class="p-3"> 14.1 By 2025, prevent and significantly reduce marine pollution of all kinds, in particular from land-based activities, including marine debris and nutrient pollution.</h3>
 +
                        <p><br /><br /></p>
 +
                        <p>We identified early on in our project that the current contraceptives are harmful to the environment, particularly the marine environment. Most of the synthetic hormones present in the current hormonal contraceptives are flushed out of the body and reach the marine environment. Ovicloak, is a non-hormonal method of contraception reducing the impact of the contraceptive to the environment. In addition, the bacteria is genetically engineered with a kill switch to prevent the bacteria from escaping to the environment, thus ensuring biosafety. In the near future, OviCloak will be instrumental in reducing the environmental pollution caused by other methods of contraception</p>
 +
                        <p>In addition to contributing to the goal through our project design, we conducted outreach activities to spread awareness on the environmental impact of the currently available contraceptives through the following programmes:</p>
 +
                        <ul>
 +
                        <li><strong>Discussion groups</strong> : We started with leading small discussion groups on this topic with the environment club of our institute called the &ldquo;<strong>Prakriti</strong>&rdquo; club. Our session with them helped them understand <strong>the cause of green contraception</strong> and through their club&rsquo;s activities, further light can be shed on this topic in the coming years.&nbsp;</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li id="5">&nbsp;<strong>Pledge and Pamphlet </strong>: On the world environment day (5 June, 2021), we circulated a brochure explaining<strong> the concepts of Green contraception</strong> and what is the correct way to dispose off condoms so as to create minimal disruption to the environment. In addition to that, to hold the stakeholders accountable, we started a pledge for using green contraception, which saw more than a<strong> 100 responses.&nbsp;</strong></li>
 +
                        </ul>
 +
                        <h2>#17: Partnerships for goals</h2>
 +
 
 +
                        <h3 style="background-color: #FFBD59;color:#8D1063 ;" class="p-3">17.6 Enhance North-South, South-South, and triangular regional and international cooperation on and access to science, technology, and innovation and enhance knowledge sharing on mutually agreed terms, including through improved coordination among existing mechanisms, in particular at the United Nations level, and through a global technology facilitation mechanism</h3>
 +
                        <p>iGEM is a one of a kind organization where people from various cultures, languages, and countries are united by just one goal - to address real world problems with the help of synthetic biology. Thus, any iGEM experience is incomplete without the spirit of collaboration and partnership.&nbsp;</p>
 +
                        <p>Team IISER-Tirupati_India has been involved in this spirit of collaboration since the beginning of our iGEM experience.&nbsp;</p>
 +
                        <ul>
 +
                        <li><strong>Syntrack</strong>: Our synthetic biology podcast, we collaborated within and across borders to facilitate the process of knowledge sharing on mutually agreeable terms. With our collaborative efforts, we were able to have discussions on relevant applications of synthetic biology that are now freely accessible to the public, becoming a right step in sharing the scientific method and its application from around the world with the world.&nbsp;</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li><strong>LAB EYES</strong> : Our collaborative spirit didn&rsquo;t stop within iGEM. For making an assistive technology software for increased accessibility to a synthetic biology laboratory, we collaborated with software engineering students.We have been in constant touch with professionals from other fields as well.</li>
 +
                        </ul>
 +
                        <ul>
 +
                        <li>Our partnerships across borders have been to facilitate the processes of synthetic biology by sharing knowledge with our fellow iGEMers. This process has been extremely fruitful as all the teams are able to gain scientific benefit and learn from each other. ( See <a href="https://2021.igem.org/Team:IISER-Tirupati_India/Partnership">partnership</a> and <a href="https://2021.igem.org/Team:IISER-Tirupati_India/Collaborations">collaborations</a> page.)</li>
 +
                        </ul>
 +
                        <p>We believe that these small steps will be an add on to sustainable development for the coming future.</p>
 
                     </div>
 
                     </div>
                  </div>
+
 
 
             </div>
 
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 +
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Revision as of 04:53, 17 October 2021


Ovi-Cloak

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“Sustainable development is the pathway to the future we want for all. It offers a framework to generate economic growth, achieve social justice, exercise environmental stewardship and strengthen governance.”

  • Ban Ki-Moon

Incorporating sustainability into innovation is the most fundamental yet most challenging aspect when developing solutions. As young scientists, concerned about the future of our planet we wanted to develop a solution that contributes to sustainable development goals. Thus, when designing OviCloak we ensured that sustainability forms the core of our project.

Through our project, we targeted to achieve 5 SDGs:

Goal #3: Good Health and Wellbeing

Goal #4: Quality Education

Goal #5: Gender Equality

Goal #14: Life below water

Goal #17: Partnership for goals

Trulli

#3 Good Health and well being

3.7 By 2030, ensure universal access to sexual and reproductive health-care services, including for family planning, information and education, and the integration of reproductive health into national strategies and programmes put in box

Ensuring universal access to sexual and reproductive health care services can contribute to significant health benefits. Ovicloak, being a contraceptive, can prevent pregnancy-related health risks in women and reduces the risk of infant and maternal mortality, by reducing the number of births and assists in child spacing.

Despite the health and non-health benefits conferred by usage of contraception, the modern contraceptive prevalence worldwide increased by only 2.1 percentage points from 55% to 57.1% between 2000 and 2009. One of the major reasons for the gradual increase in the usage of modern contraceptives is the fear of side-effects which deter women from using contraceptives leading to unwanted pregnancies negatively impacting their physical and mental health.

Our project, Ovicloak is designed to be a long term reversible contraceptive with minimal side effects. In addition, the reversibility of the contraceptive allows an individual to regulate and control their fertility. These attributes of Ovicloak can encourage women who do not want to conceive to use contraceptive decreasing the risk of unwanted pregnancies.

The knowledge about sexual and reproductive health plays a pivotal role in the accessibility of its health services. Thus, our team contributed to enhancing sexual and reproductive health-care services though two strategies 

  1. Collecting data regarding the status of awareness on Reproductive Health and Rights 
  • Reproductive Health and Rights Survey: The survey was conducted with the intention of understanding about the extent of awareness on Reproductive Health and Rights and identifying the major sources of sexuality education in India. The survey included participation of 633 participants. (See Education)
  • In depth Interview: We conducted an in depth Interview with 8 participants to learn about the general opinion of people on existing contraceptives and their perspective on the need for new contraceptives. This allowed us to obtain qualitative data and understand the respondent’s perspective, feelings and experience. (See Education)
  1. Conducting Sexual and Reproductive Health awareness sessions
  • Shush the Hush: The webinar series was conducted to facilitate discussion on Reproductive Health and Rights, and saw 550+ registrations in total. Due to the conservative nature of Indian society topics surrounding reproductive and sexual health are not widely discussed leading to misconceptions.The session was focussed on debunking myths and misconceptions associated with reproductive and sexual health and educating the public on contraceptive options and safe sexual practices.The webinar conducted received a positive feedback from the participants for the informative session. (See Education).
  • Ask about it: The Instagram live series was launched with an intention to promote discussion on Menstrual Health, STI and UTI in the public. The session allowed us to engage with the public actively and break the stigma surrounding these topics. The live series received over 560+ views in total. (See Education)
  • That skipped chapter; An Instagram Reel series,through which we communicated with the audience about Sexually transmitted Infection (STI), the importance of consent when engaging in sexual activity, representation of sex in media and porn and the myth surrounding the association of hymen with virginity.
  • The birds and bees: An Art Exhibition: We put out an art exhibition on the theme of World Contraception Day “Let every pregnancy be a planned one” and got artists from all over the country and the world to present their artwork. We received entries in the form of traditional

    art and paintings which were posted on our social media pages. (See Education)



#4 Quality education 

4.5 By 2030, eliminate gender disparities in education and ensure equal access to all levels of education and vocational training for the vulnerable, including persons with disabilities, indigenous peoples, and children in vulnerable situations

Our project OviCloak is based on providing a synthetic biology approach to contraception. We undertook this UN sustainable development goal as we planned to educate the people around us on the different aspects of Synthetic Biology. 

Synthetic biology has the potential to eradicate the rampant lack of awareness of Biological processes and the scientific method and society at large. The solutions provided by synthetic biology resonate with people and their problems and thus, synthetic biology can cater to the educational needs of people from all backgrounds and become an effective tool to eliminate gender disparities, provide vocational training equally at all levels and uplift the children in vulnerable situations. 

We realised the goal through the following activities:

  • Gene Gala: The 5-day Mini Summer School was a special effort by team IISER-Tirupati_India in collaboration with team IISER Kolkata to end gender disparity in the field of STEM. Gene Gala saw the constant participation of about 45-50 girl students from high schools under the Directorate of Education, GNCT Delhi. The girl students were able to grasp the concepts of synthetic biology well and had a positive learning experience with our teams. See Education
  • Translation of Science: An interactive zine, regarding microbiome was created and translated to a local language Hindi.This exposed students to a new arena of science in a fun mode.It was also accessible to students studying science in schools with the Hindi medium of instruction. See Education
  • Sign Language: To increase access of synthetic biology to people who are hard of hearing, we translated our promo video in Sign language, using STEM signs to explain the processes. It helped open up a dialogue between science and the deaf community. See See Education
  • LAB EYES: To increase synthetic biology laboratory access to visually impaired individuals, we collaborated with two software engineering graduates from India to design and implement an assistive technology software. This software allows the identification of chemicals and consumables without resorting to touching, smelling, or tasting them.See Education 
  • Agastya: Our team conducted a virtual synthetic biology workshop session with students from the Navodaya schools through the Agastya foundation. The pandemic has been hard on the

    student community, depriving such students of excellence the right exposure to the scientific method. Our workshop introduced them to the basics of the scientific method, the tools, and techniques of synthetic biology and ended with a holistic discussion on the process of biological contraception and introduction to our project.See Education.

#5: Gender Equality

UNICEF have found that Girls are less likely than boys to achieve high proficiency levels in STEM and at upper secondary, fewer girls choose STEM majors. 

As undergraduates pursuing science and researching in synthetic biology : a more advanced field of science, we tried to achieve Gender Equality in STEM through following collaborations with other iGEM teams.

  • Gene Gala : A 5-day Mini Summer School in collaboration with iGEM Team of IISER Kolkata, for 45-50 girl students from high schools under the Directorate of Education, GNCT Delhi.The girl students were able to grasp the concepts of synthetic biology,

See Education

  • Femme club: A club initiative of teams Moscow City and Team LMSU, where female members of iGEM teams come together and discuss the various challenges faced by women in the field of STEM. This helped us act collectively to overcome the barriers in future.
  • Rosalind Chronicles: a collaborative initiative by the team of iGEM ULaval iGEM Concordia-Montreal and iGEM Patra, which documented inspiring woman scientist and female team members of each team.This helped us reflect upon our own team and realise the invaluable role of women in STEM.

5.6 Ensure universal access to sexual and reproductive health and reproductive rights as agreed in accordance with the Programme of Action of the International Conference on Population and Development and the Beijing Platform for Action and the outcome documents of their review conferences

UNFPA says: “Family planning is central to gender equality and women’s empowerment, and it is a key factor in reducing poverty.” They found that when women and couples are empowered to plan their family, women are better able to complete their education; women’s autonomy within their households is increased; and their earning power is improved.Thus leading to empowerment of women. OviCloak, being a long term reversible contraceptive with least to side effects, was aimed to ensure gender equality and empower women by allowing women to regulate their fertility.

The UN's World Contraceptive Day (WCD) coalition agrees that addressing gender inequality is one of the four areas that needs development globally to avoid/reduce unplanned pregnancy. This dependence between family planning and gender equality was striking.

Through meetings with stakeholders like Ms.Kathy Walking and Ms.Nalini Jameela, we understood that to empower girls and young women with the knowledge they need to take control of their sexual and reproductive health, which we did through above mentioned activities like Shush the Hush, Ask about it, That skipped chapter, and The birds and bees.

In addition to achieving equality among binary genders.We also, took a step to address the inequality in the minority genders by addressing the contraceptive needs of transmasculine spectrum. This was a result of meeting with our stakeholders Dr.Frances Grimstad and NGO-Sex Education.India.Hence, we believe this indeed is a novel step towards achieving this SDG.

#14: Life below water

14.1 By 2025, prevent and significantly reduce marine pollution of all kinds, in particular from land-based activities, including marine debris and nutrient pollution.



We identified early on in our project that the current contraceptives are harmful to the environment, particularly the marine environment. Most of the synthetic hormones present in the current hormonal contraceptives are flushed out of the body and reach the marine environment. Ovicloak, is a non-hormonal method of contraception reducing the impact of the contraceptive to the environment. In addition, the bacteria is genetically engineered with a kill switch to prevent the bacteria from escaping to the environment, thus ensuring biosafety. In the near future, OviCloak will be instrumental in reducing the environmental pollution caused by other methods of contraception

In addition to contributing to the goal through our project design, we conducted outreach activities to spread awareness on the environmental impact of the currently available contraceptives through the following programmes:

  • Discussion groups : We started with leading small discussion groups on this topic with the environment club of our institute called the “Prakriti” club. Our session with them helped them understand the cause of green contraception and through their club’s activities, further light can be shed on this topic in the coming years. 
  •  Pledge and Pamphlet : On the world environment day (5 June, 2021), we circulated a brochure explaining the concepts of Green contraception and what is the correct way to dispose off condoms so as to create minimal disruption to the environment. In addition to that, to hold the stakeholders accountable, we started a pledge for using green contraception, which saw more than a 100 responses. 

#17: Partnerships for goals

17.6 Enhance North-South, South-South, and triangular regional and international cooperation on and access to science, technology, and innovation and enhance knowledge sharing on mutually agreed terms, including through improved coordination among existing mechanisms, in particular at the United Nations level, and through a global technology facilitation mechanism

iGEM is a one of a kind organization where people from various cultures, languages, and countries are united by just one goal - to address real world problems with the help of synthetic biology. Thus, any iGEM experience is incomplete without the spirit of collaboration and partnership. 

Team IISER-Tirupati_India has been involved in this spirit of collaboration since the beginning of our iGEM experience. 

  • Syntrack: Our synthetic biology podcast, we collaborated within and across borders to facilitate the process of knowledge sharing on mutually agreeable terms. With our collaborative efforts, we were able to have discussions on relevant applications of synthetic biology that are now freely accessible to the public, becoming a right step in sharing the scientific method and its application from around the world with the world. 
  • LAB EYES : Our collaborative spirit didn’t stop within iGEM. For making an assistive technology software for increased accessibility to a synthetic biology laboratory, we collaborated with software engineering students.We have been in constant touch with professionals from other fields as well.
  • Our partnerships across borders have been to facilitate the processes of synthetic biology by sharing knowledge with our fellow iGEMers. This process has been extremely fruitful as all the teams are able to gain scientific benefit and learn from each other. ( See partnership and collaborations page.)

We believe that these small steps will be an add on to sustainable development for the coming future.

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