Team:Wageningen UR/Parts

iGEM Wageningen 2021

Biobricks

Photo of centrifuge with colorfull eppendorf tubes

This page contains all the Biobrick Parts created for Cattlelyst. All these parts can be found on the iGEM Registry of Standard Biological Parts. This list also contains the biobricks we submitted as best basic part (BBa_K3747607), composite part (BBa_K3747606), and part collection (Denitrification).

Methane Oxidation

Table 1: Parts related to Particulate methane monooxygenase (pMMO)
Biobrick Code Part Name Part Type Designer
BBa_K3747005 Particulate methane monooxygenase substructure B1 Basic Riemer & Morris
BBa_K3747007 Particulate methane monooxygenase substructure B2 Basic Riemer & Morris
BBa_K3747008 Apo form of human heavy-chain ferritin Basic Riemer & Morris
BBa_K3747016 T7 terminator Basic Riemer & Morris
BBa_K3747015 Particulate methane monooxygenase mimic m1 Composite Riemer & Morris
Table 2: Parts related to Soluble methane monooxygenase (sMMO)
Biobrick Code Part Name Part Type Designer
BBa_K3747019 Soluble methane monooxygenase Composite Riemer
BBa_K3747017 GroS chaperone Basic Riemer
BBa_K3747018 GroL chaperone Basic Riemer
BBa_K3747020 GroES chaperone Basic Riemer
BBa_K3747021 GroEL-2 chaperone Basic Riemer
BBa_K3747004 J231XX promotor Basic Riemer
BBa_K3747022 Chaperone insert Composite Riemer

Denitrification

Table 3: Parts from Pseudomonas stutzeri JM300
Biobrick Code Part Name Part Type Designer
BBa_K3747100 Periplasmic nitrate reductase E Basic Sophieke
BBa_K3747101 Periplasmic nitrate reductase D Basic Sophieke
BBa_K3747102 Periplasmic nitrate reductase A Basic Sophieke
BBa_K3747103 Periplasmic nitrate reductase B Basic Sophieke
BBa_K3747104 Periplasmic nitrate reductase C Basic Sophieke
BBa_K3747137 NapEDABC Composite Sophieke
BBa_K3747600 Nap operon Basic Sanne
BBa_K3747601 Nir operon Basic Sanne
BBa_K3747602 Nor operon Basic Sanne
BBa_K3747603 Nos operon Basic Sanne
BBa_K3747117 Nitrous oxide reductase R Basic Sophieke
BBa_K3747118 Nitrous oxide reductase Z Basic Sophieke
BBa_K3747119 Nitrous oxide reductase D Basic Sophieke
BBa_K3747120 Nitrous oxide reductase F Basic Sophieke
BBa_K3747121 Nitrous oxide reductase Y Basic Sophieke
BBa_K3747122 Nitrous oxide reductase L Basic Sophieke
BBa_K3747123 Twin-arginine translocation A Basic Sophieke
BBa_K3747140 NosRZDFYLTatA Composite Sophieke
BBa_K3747124 ApbE Basic Sophieke
BBa_K3747125 Blue copper protein 1 Basic Sophieke
BBa_K3747126 Blue copper protein 2 Basic Sophieke
BBa_K3747127 Cytochrome c550 Basic Sophieke
BBa_K3747141 ApbE-blue1-blue2-c550 Composite Sophieke
BBa_K3747133 NirQ Basic Anemoon
BBa_K3747134 NirO Basic Anemoon
BBa_K3747135 NirP Basic Anemoon
BBa_K3747144 NirQOP accessory Composite Anemoon
BBa_K3747128 Nitrite reductase K Basic Anemoon
BBa_K3747129 Nitrite reductase V Basic Anemoon
BBa_K3747142 NirKV Composite Anemoon
BBa_K3747130 Nitric oxide reductase C Basic Anemoon
BBa_K3747131 Nitric oxide reductase B Basic Anemoon
BBa_K3747132 Nitric oxide reductase D Basic Anemoon
BBa_K3747143 NorCBD Composite Anemoon
BBa_K3747606 Bacterial Artificial Chromosome (BAC) Composite Sanne
Table 4: Parts from Cupriavidus necator H16
Biobrick Code Part Name Part Type Designer
BBa_K3747105 Periplasmic nitrate reductase E Basic Sophieke
BBa_K3747106 Periplasmic nitrate reductase D Basic Sophieke
BBa_K3747107 Periplasmic nitrate reductase A Basic Sophieke
BBa_K3747108 Periplasmic nitrate reductase B Basic Sophieke
BBa_K3747109 Periplasmic nitrate reductase C Basic Sophieke
BBa_K3747138 NapEDABC Composite Sophieke
Table 5: Parts from Paracoccus denitrificans DSM 413
Biobrick Code Part Name Part Type Designer
BBa_K3747110 Periplasmic nitrate reductase E Basic Sophieke
BBa_K3747111 Periplasmic nitrate reductase D Basic Sophieke
BBa_K3747112 Periplasmic nitrate reductase A Basic Sophieke
BBa_K3747113 Periplasmic nitrate reductase B Basic Sophieke
BBa_K3747114 Periplasmic nitrate reductase C Basic Sophieke
BBa_K3747115 Periplasmic nitrate reductase G Basic Sophieke
BBa_K3747116 Periplasmic nitrate reductase H Basic Sophieke
BBa_K3747139 NapEDABCGH Composite Sophieke
Table 6: Parts from Pseudomonas putida EM42
Biobrick Code Part Name Part Type Designer
BBa_K3747607 Spacer targeting promoter Cyo oxidase Basic Sanne
Table 7: Synthetic parts
Biobrick Code Part Name Part Type Designer
BBa_K3747604 Lox site lox66 Basic Sanne
BBa_K3747605 Lox site lox2m/71 Basic Sanne

Safety

Table 8: Parts related to the methane or proximity dependent kill switch
Biobrick Code Part Name Part Type Designer
BBa_K3747300 Plux/lac_dE hybrid promotor Basic Sophie
BBa_K3747301 Plux/lac_dF hybrid promotor Basic Sophie
BBa_K3747201 Reporter-pR-GFP Composite Jenny
BBa_K3747202 Positive control-pR-GFP Composite Jenny
BBa_K3747203 Reporter-pL-RFP Composite Jenny
BBa_K3747204 Positive control-pL-GFP Composite Jenny
About Cattlelyst

Cattlelyst is the name of the iGEM 2021 WUR team. Our name is a mix of 1) our loyal furry friends, cattle, and 2) catalyst, which is something that increases the rate of a reaction. We are developing “the something” that converts the detrimental gaseous emissions of cattle, hence our name Cattlelyst.

Are you curious about our journey? We have written about our adventures in our blog, which you can find here:

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