Team:Wageningen UR/Notebook/Protocols

iGEM Wageningen 2021



Find below the protocols we used for our experiments.

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Conjugation Gibson Assembly Golden Gate Assembly PCR and variations Electroporation Heat shock transformation of E. coli SDS-PAGE Growth Experiments Griess/Nitrite Assay Nitrate Assay
About Cattlelyst

Cattlelyst is the name of the iGEM 2021 WUR team. Our name is a mix of 1) our loyal furry friends, cattle, and 2) catalyst, which is something that increases the rate of a reaction. We are developing “the something” that converts the detrimental gaseous emissions of cattle, hence our name Cattlelyst.

Are you curious about our journey? We have written about our adventures in our blog, which you can find here: