Team:USP-EEL-Brazil/Contribution
As a contribution to all iGEM, Synthetic Biology, and related communities, we have added a Coding Sequence part on Registry of our γPLI, and also of our PLA2 of Bothrops jararaca. By providing these parts with all data and already optimized (for E. coli) we hope to contribute to future research on Ophidic Accidents, a field still neglected in Brazil.
On modeling we have predicted a model by homology, that is important to future research, on docking or molecular simulations dynamics, to se how the inhibitor works in molecular levels, and the dynamic simulation to see how the inhibitor would behave in differents situations and medium.
Since snakebites accidents are neglected by health organisations, to provide a new way to avoid permanent damage due to snake venom intoxication is extremely important. Nowadays, plenty animals suffer due the traditional way to make snake venom serum, with that being said, we believe that by making an inhibitor using animal free technology is the first step of making a completely synthetic serum. We hope that our research help future professionals to elaborate this, since we are not able to push our project as far as it need to go. We found some tridimentional structures of the PLI by our structural modeling, it means that it will be helpful to future researchers aiming to understand the active site, molecular properties and energetic values of this protein. Besides that, we found out that, we added our biological circuit to the registry, facilitating the production of this vital inhibitor. We also contributed with health professionals, since someday, grave accidents involving snakebites will no longer led to amputation and necrosis, our way to storage the inhibitor will allow a quicker treatment.
Due to the absence of γPLI Crystallography data, our predicted model, presents even more importance and impact on future research regarding Ophidic Accidents Therapeutics.