Team:UCAS-China/Parts

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• Parts
• Basic Parts
• Composite Parts

This year we have designed 18 parts by ourselves. Among these parts, 12 are basic parts and others are composite parts. Due to some parts have been designed by previous teams, so we quote their parts in the design of our composite parts. The function, the safety, the efficiency and other factors are considered seriously to ensure they can execute according to our original design. You can click on each part’s name to learn more about our parts and our project.

Basic Parts

Basic parts include the promoters, the primers, the coding areas and the tags. After referring to many references and the principles of gene designing, we download the gene files from references and NCBI. According to our requests to these genes, we redesign some parts of them in order to get better adaptability. Even though the amount of parts is less than others teams, they can execute their functions in an efficient way. The following is our table of basic parts.

Number	Type	Description	Designer	Length
BBa_K3817000	Coding	CkTcS enzyme	Ji Sun	1113
BBa_K3817001	Coding	CkTcS-new enzyme	Ji Sun	1107
BBa_K3817002	Tag	6His-thrombin site-SUMO tag	Ji Sun	351
BBa_K3817003	T7	T7 promoter-lac operator-RBS	Ji Sun	81
BBa_K3817004	Coding	Cdh enzyme	Ji Sun	3768
BBa_K3817005	Coding	Ptrc temp-sensitive promoter	Ji Sun	521
BBa_K3817006	Translational unit	PUTRs-rplt	Bowen Liu	386
BBa_K3817007	Coding	cAMP receptor protein (CRP)	Bowen Liu	633
BBa_K3817008	Primer	Pbluescript II SK+ MCS forward primer	Ji Sun	20

Table 1. Basic Parts of Decaffi

Composite Parts

All composite parts serve for the protein expression which is our eventual goal. We combine the coding areas with promoter, making them the functional units. The sources of inspiration come from E. coli and Camellia assamica var. kucha.

To establish the efficient pathway, we combine PUTRs-rplt promoter with coding areas. To obtain purer enzyme, we use 6×his and SUMO double-tag. To ensure the biosafety, we combine temperature sensitive promoter with toxin-antitoxin system. Besides, We also tested gene expression for coenzyme production in yeast to enable us to be self-sufficient in enzymes from materials when we realize factorization. The following is our table of composite parts.

Number	Type	Description	Designer	Length
BBa_K3817011	Composite	CkTcS pathway: T7promoter-lac operator-RBS + 6His-thrombin site-SUMO tag + CkTcS enzyme	Ji Sun	1559
BBa_K38170012	Composite	CkTcS new_pathway: T7promoter-lac operator-RBS + 6His-thrombin site-SUMO tag + CkTcS new_enzyme	Ji Sun	1539
BBa_K3817014	Composite	Cdh pathway: T7promoter-lac operator-RBS + 6His-thrombin site-SUMO tag + Cdh enzyme	Ji Sun	4200
BBa_K3817015	Composite	Ptrc toxin-antitoxin safety module: RelE toxin + Ptrc temp-sensitive promoter + RelB antitoxin	Ji Sun	1108
BBa_K3817017	Composite	SAM2-CBS inserted by Bleomycin pathway: SAM2 + CBS inserted by Bleomycin	Bowen Liu	3054
BBa_K3817018	Composite	PUTRs-rplt-CRP pathway: PUTRs-rplt + cAMP receptor protein (CRP)	Bowen Liu	1019
BBa_K3817006	Translational unit	PUTRs-rplt	Bowen Liu	386

Table 2. Composite Parts of Decaffi