You can click on the orange links to download our protocols.
Here is the general protocols of our Basic experiments.
Protein production and purification
This is how we product and purify our proteins.
We use directed evolution to get the targeted protein.
We use bicinchoninic acid (BCA) to measure the concentration of protein, therefore we can further verified that we obtained the target protein and analyzed its physicochemical properties.
We use CPM assay to measure the thermal stability of our enzymes--including wild-type and mutant ones.
Use of HPLC
We use the principles of high performance liquid chromatography (HPLC) to analyse organic compounds with high boiling points that are not volatile, heat unstable and have a high molecular weight.
Biosynthesis of SAM
Here is how we make adenosine methionine (SAM).
We tested and evaluated the immobilized enzyme.