Team:UCAS-China/Experiment

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• Experiment
• Basic experiment
• Protein production and purification
• Directed evolution
• BCA assay
• CPM assay
• Use of HPLC
• Biosynthesis of SAM
• Enzyme immobilization

You can click on the orange links to download our protocols.

Basic experiments

Here is the general protocols of our Basic experiments.

Protein production and purification

This is how we product and purify our proteins.

Directed evolution

We use directed evolution to get the targeted protein.

BCA assay

We use bicinchoninic acid (BCA) to measure the concentration of protein, therefore we can further verified that we obtained the target protein and analyzed its physicochemical properties.

CPM assay

We use CPM assay to measure the thermal stability of our enzymes--including wild-type and mutant ones.

Use of HPLC

We use the principles of high performance liquid chromatography (HPLC) to analyse organic compounds with high boiling points that are not volatile, heat unstable and have a high molecular weight.

Biosynthesis of SAM

Here is how we make adenosine methionine (SAM).

Enzyme immobilization

We tested and evaluated the immobilized enzyme.