Our project aims to find an ideal framework for protein overexpression through the outer membrane protein (eCPX) system. The following table lists the new parts created by the 2021 UBrawijaya iGEM team.
| Diagram | Part Registry ID | Part Type | Part Name | Usage |
|---|---|---|---|---|
 |
BBa_K4080006
http://parts.igem.org/Part:BBa_K4080006 |
Coding | eCPX (Enhanced Circularly Permuted Outer Membrane Protein X) | @Improved the presentation of diverse passenger peptides on the cell surfaces |
 |
BBa_K4080007
http://parts.igem.org/Part:BBa_K4080007 |
Composite | amilCP-eCPX fusion with universal expression | One of two main systems designed to maximize the production levels of the chromoprotein to overexpression under a constitutive promoter |
 |
BBBa_K4080008
http://parts.igem.org/Part:BBa_K4080008 |
Composite | TEV protease-eCPX fusion with universal expression | Expressed TEV protease through constitutive promoter and translocates TEV protease across the cell membrane and out of the cell under OmpX signal sequence |
 |
BBBa_K4080009
http://parts.igem.org/Part:BBa_K4080009 |
Composite | Sec system (PhoA), TEV protease with universal expression | Expressed TEV protease through constitutive promoter and translocates TEV protease across the cell membrane and out of the cell through the Sec pathway |
 |
BBa_K4080010
http://parts.igem.org/Part:BBa_BBa_K4080010 |
Composite | amilCP-eCPX fusion with T7 expression | One of two main systems designed to maximize the production levels of the chromoprotein to express overexpression under T7 promoter |
 |
BBa_K4080011
http://parts.igem.org/Part:BBa_K4080011 |
Composite | TEV protease-eCPX fusion with T7 expression | Expressed TEV protease through T7 promoter and translocates TEV protease across the cell membrane and out of the cell under OmpX signal sequence |
 |
BBa_K4080012
http://parts.igem.org/Part:BBa_K4080012 |
Composite | TSec system (PhoA), TEV protease with T7 expression/td> | Expressed TEV protease through T7 promoter and translocates TEV protease across the cell membrane and out of the cell through the Sec pathway |
The following table lists the existing parts used by the 2021 UBrawijaya iGEM team to create the CHOP system. We added information from the literature regarding the part listed below as a form of contribution
| Diagram | Part Registry ID | Part Type | Part Name | Usage |
|---|---|---|---|---|
 |
BBa_K880005
http://parts.igem.org/Part:BBa_K880005 |
Generator | Constitutive promoter family member-RBS | Protein-generator in the universal expression system |
 |
BBa_K1614000
http://parts.igem.org/Part:BBa_K1614000 |
Regulatory | T7 promoter | Regulated the gene expression of recombinant proteins in the T7 expression system as part of the characterization |
 |
BBa_B0034
http://parts.igem.org/Part:BBa_B0034 |
RBS | RBS (Elowitz 1999) | Assembled efficient coding BioBricks and regulate their expression under the T7 expression system |
 |
BBa_K3128050
http://parts.igem.org/wiki/index.php?title=Part:BBa_K3128050 |
Coding | OmpX signal sequence | Prompt a cell to translocate extracellular the targeted proteins in the construct that used eCPX fusion |
 |
BBa_K1507019
http://parts.igem.org/Part:BBa_K1507019 |
Coding | PhoA signal sequence | Prompt a cell to translocate extracellularly the targeted proteins through the Sec pathway |
 |
BBa_K592009
http://parts.igem.org/Part:BBa_K592009 |
Coding | amilCP | Targeted protein expressed by our system. Afterwards, the result from blue chromoprotein acts as a reporter gene to indicate our system is working |
 |
BBa_J18918
http://parts.igem.org/Part:BBa_J18918 |
Protein_domain | TEV cleavage site | Act as a linker between chromoprotein and eCPX. When the outer membrane vesicle has been produced then this linker will be cleaved by TEV protease and chromoprotein will be released |
 |
BBa_K1319004
http://parts.igem.org/Part:BBa_K1319004 |
Coding | TEV protease | Cleaved the fusion proteins at the designed cleavage site |
 |
BBa_B1006
http://parts.igem.org/Part:BBa_B1006 |
Terminator | Terminator (artificial, large, %T~>90) | Encoded an RNA sequence that can form a stable stem-loop structure to define the end of a transcriptional unit |
|
BBa_B0012
http://parts.igem.org/Part:BBa_B0012 |
Terminator | T7 terminator | Encoded an RNA sequence that can form a stable stem-loop structure to define the end of a transcriptional unit |