Team:SMS Shenzhen/Parts
Basic Parts
| Part Number | Part Name | Function | Discription |
|---|---|---|---|
| BBa_K3990001 | LCP-K30(Codon optimizated) | decompose Polyisoprene | We will express the enzyme LCP in BL21(DE3) E.coli to decompose the polyisoprene. |
| BBa_K3633007 | LaccaseCotA | oxidize ABTS | We will express the enzyme LaccaseCotA in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K3990002 | LaccaseCotA-497 | oxidize ABTS | We will express the enzyme LaccaseCotA (A:HIS497>ASP) in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K3990003 | LaccaseCotA-386 | oxidize ABTS | We will express the enzyme LaccaseCotA (A: LEU386>GLN) in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K3990004 | LaccaseCotA-386-497 | oxidize ABTS | We will express the enzyme LaccaseCotA (A: LEU386>GLU, HIS497>ASP) in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K3990005 | LaccaseCotA-2-8 | oxidize ABTS | We will express the enzyme LaccaseCotA (2-8) in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K3990006 | LaccaseCotA-2-12 | oxidize ABTS | We will express the enzyme LaccaseCotA (2-12) in BL21(DE4) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). |
| BBa_K1819003 | RoxA | decompose Polyisoprene | We document the existing part. |
| BBa_K3990015 | RoxB | decompose Polyisoprene | We document the a part. |
| BBa_K1819001 | LCP | decompose Polyisoprene | We document the existing part. |
Composite Parts
| Part Number | Part Name | Function | Discription | Sequence |
|---|---|---|---|---|
| BBa_K3990007 | T7+LCP Expressing Construct | decompose the polyisoprene | We will express the enzyme LCP in BL21(DE3) E.coli to decompose the polyisoprene. | BBa_K525998, BBa_K3990001 |
| BBa_K3990008 | T7+Laccase CotA Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3633007 |
| BBa_K3990009 | T7+Laccase CotA H497D Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA ((A:HIS497>ASP)) in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3990002 |
| BBa_K3990010 | T7+Laccase CotA L386E Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA (A: LEU386>GLN)) in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3990003 |
| BBa_K3990011 | T7+Laccase CotA [H497D; L386E] Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA (A: LEU386>GLU, HIS497>ASP) in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3990004 |
| BBa_K3990012 | T7+Laccase CotA [T31I; V104A; Y141C; R146C; R198H; L206Q; L355P; L475P] Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA (2-8) in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3990005 |
| BBa_K3990013 | T7+Laccase CotA [E34G; T87A; L182R] Expressing Construct | oxidize ABTS | We will express the enzyme LaccaseCotA (2-12) in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA). | BBa_K525998, BBa_K3990006 |