Team:NUS Singapore/Engineering

iGEM Wiki

iGEM Wiki


Our goal for engineering the blue light system was to implement a system that was of practical use when coupled with our flocculation system.
1. Strong enough to completely flocculate the culture in a reasonable amount of time
2. Not overly leaky to prevent unwanted flocculation

To achieve this, we utilized the Design-Build-Test-Learn cycle to progressively improve our system over several iterations.

Design: Using acquired knowledge either through experimentation or literature, a rational plan is devised for implementing our system.

Build: High efficiency cloning methods are utilized to forge genetic constructs and develop strains to test our systems.

Test: Systems are characterized and quantified with the scientific method.

Learn: Conclusions are drawn on our design and used to inform our next iteration of the cycle.