Team:NUDT CHINA/Parts Collection

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PART COLLECTION

Introduction

Here we present a collection of parts that can assemble Predator system under the induction of external signals such as small molecule rapamycin and blue light. Therefore, controllable protein degradation can be achieved through different combination of the parts from this collection.

In 2020, we designed RiPrePro system which is mediated by small molecule thus lead to the degradation of target modules. This year, the new system LiPrepro was designed and testyfied to have great combination with the targeted Cycleblue parts and make the cell cycle under the control of blue light.

The resemblance between two systems is the mechanism of ubiquitination. However, they are different in the "switch" of the system--small molecules and bule light. Small molecules could function broadly in side our body while the bule light is a much quicker and cheaper way to control the cell cycle. They are all useful tools.

Part Collection List

LiPrePro


1-1 BBa_K4016040 Trim21-3xGS linker-CRY2 1-2 BBa_K4016042 Trim21-5xGS linker-CRY2
1-3 BBa_K4016035 Trim21-CRY2 1-4 BBa_K4016041 GFPnano-3xGS linker-CIB1
1-5 BBa_K4016043 GFPnano-5xGS linker-CIB1

CycleBlue

2-1 BBa_K4016031 2×Rb CHelix-CIB1 2-2 BBa_K4016027 2×CDK4 αCHelix-CIB1 linker-CRY2