Experiment
LB Medium Preparation
Agarose Gel Electrophoresis
PCR
OE-PCR
Colony PCR
Transformation
Double Enzyme DNA Digestion
DNA Ligation
Gel Purification
Plasmid Conservation
Plasmid Extraction
SEAP Assay In Vitro
Transfection
CCK-8 Assay
BCA_protein_assay
Western Blotting