Team:NJTech China/Attributions

• Home
• Project
  • Description
  • Design
  • Perspective
  • Modeling
  • Proposed Implementation
• Achievements
  • Contribution
  • Engineering
  • Parts
  • Judging Form
• Team
  • Team
  • Attributions
• HP
  • HumanPractices
  • Collaborations
  • Partnership
  • Communication
  • Excellence in Another Area
• Safety
• Lab
  • Notebook
  • Protocols
  • Experiments
  • Results

Attributions

The Nanjing Tech University iGEM team was responsible for almost all aspects of running the team, from including purchasing supplies, coming up with the project concept, writing protocols, and executing the lab work. We would like to thank all our advisors who have assisted us throughout the project. We would also like to thank all everyone else who has helped us realize this project, be it through invaluable advice or providing DNA, strains, or other materials. These contributions have helped us enormously.

Thanks to

Team members

Yujiao Wang

Responsible for experimental group work; Designed the GFP reporting pathways of sythetic promoters; Designed the bronze medal and sliver medal experiment; Provided material for wiki, presentation and communication.

Yiqing Zhang

Responsible for experimental group work; Designed and constructed the GFP reporting pathways of sythetic promoters; Provided material for wiki, presentation and communication.

Senlin Zhong

Participated in the design and expression of gfasPurple protein; Provided wiki material of the bronze prize; Insert literature sources for the final pages.

Hongliang Long

Participated in the construction of S. cerevisiae containing pRS426-Rosa and pRS406- Rosa recombinant plasmids; Provided materials for presentation and communication.

BiKun Chi

Write Experimental records of PCR validation of strain pRS426-Petunia, pRS426-Rosa, pRS426-Vanda; Do the fermentation experiment of wild type strains; Measure Glucose content and OD value of strain WT and analysis the production of 2-PE by HPLC.

Yiping Cao

Constructed the target strain (S. cerevisiae BY4741-pRS426-Petunia, S. cerevisiae BY4741-pRS426-Vanda, S. cerevisiae BY4741-pRS426-Rosa) producing 2-PE; Performed fermentation experiment (S. cerevisiae BY4741-pRS426-Petunia) and tested 2-PE concentration by HPLC; Provided material for presentation and communication.

Chenyu Wang

Introduced the plasmids into S. cerevisiae BY4741 and verified; Used the transforming strain to produce 2-PE and measured the yield of 2-PE.

Jikun Wang

Built the growth model to describe the growth of yeast, the production of phenylethanol, and the consumption of substrates; Established the expansion model to describe the growth and expansion of yeast in the natural environment.

Qingying Liu

Characterized of aeblue protein; Participate in Hp activities, read literature and answer questions from other universities; Provided wiki material of the bronze prize.

Dongjing Wang

Characterized of aeblue protein; Participate in Hp activities, read literature and answer questions from other universities; Provided wiki material of the bronze prize.

Zihan Teng

Wrote the script of yeast animation; In educational institutions, lectures were given to middle and high school students and 60 questionnaires were distributed offline; Wrote part of the hp material and participated in the dance shooting.

YuXuan Tang

Responsible for human practices group work and science communication; Communicated with companies and other iGEM teams; Provided material for presentation and communication; Responsible for promotion video; Designed questionnaire; Drew pictures for promotion video, poster, wiki; Design a set of internet meme; Responsible for wiki design.

YiFan Jiang

Editing promotion video.

Yuanzhang Li

Understand the structure of the web page; be familiar with the code; and determine the upload server method.

Jiaying Hu

Responsible for financial affairs; Transformed plasmids into DH5α E.coli; Transformed plasmids into S.cerevisiae BY4741; Fermented the yeast with the plasmid pRS426-Vanda in shaking flask and measured the yield of 2- Phenylethanol through HPLC; Constructed a yeast-microalgae interaction system and bioreactor by 3D Printer; Participated in writing wiki material.

Ziyan Li

Transformed plasmids into DH5α E.coli; Recombined three genes into S.cerevisiae BY4741, Vanda、Rosa and Petunia. Fermented yeast in shaking flask with the gene Vanda and measured the yield of phenylethanol through HPLC. Constructed a yeast-microalgae interaction system and bioreactor by 3D Printer. Provided material for Description.

Cheng Chen

Transformed plasmids into DH5α E.coli; Recombined three genes into yeast, Vanda、Rosa and Petunia; Fermented yeast in shaking flask with the gene Rosa and measured the yield through HPLC; Provided material for Engineering Success.

Didi Feng

Transformed plasmids into DH5α E.coli; Recombined three genes into yeast, Vanda、Rosa and Petunia; Fermented yeast in shaking flask with the gene Rosa and measured the yield through HPLC; Provided material for Description.

Wenbo Zhu

Involved in the extraction and purification of aeblue protein induced expression proteins as well as for protein concentration, wavelength measurement, and protein electrophoresis; Participated in brewer's yeast fermentation and performed HPLC analysis of substrate and product concentrations.

YiFei Dai

Organizer and Leader of the model group; Responsible for the data analyzing and cleaning; Using python to construct the basic structure of the Neural Network model; Tuning and testing the CNN, RNN and Random Forest model for further improvment.

Jvzheng Liang

Responsible for data processing and programming; Do mainly python coding and modeling based on neural network machine learning including Random Forest; Convolutional Neural Network and Recurrent Neural Network.

Yuting Zeng

Responsible for the bronze medal experiment: Characterized the chromoprotein gfasPurple; Responsible for the gold medal experiment: Constructed four recombinant plasmids; Provided wiki material of the bronze prize and gold medal.

Huixin Hou

Responsible for the bronze medal experiment: Characterized the chromoprotein gfasPurple; Responsible for the gold medal experiment: Constructed four recombinant plasmids; Provided wiki material of the bronze prize and gold medal.

JiFan Zhang

Communicated with companies and other IGEM teams; Visited a farm in Nanjing; Designed and distributed a questionnaire; Participated in Nanjing China-iGEM’s meet up activity and presented our projects to other teams; Provided wiki materials for HP.

Advisors

Changsheng Xuan

Clean up old wiki code and modify page layout; Gather information about the animation effects of the page; Organize what needs to be done and familiarize yourself with the site.

Wei Yan

Provided experimental operation guidance.

Feng Guo

Provided experimental operation guidance.

General Support

Thanks to our advisors for their help in team running, project guidance and experimental support. Here is their contribution.

Lab support

Our Primary PI, Dr.Wenming Zhang provided us with support of experiment platform, materials and equipment. He arranged the overall wet-lab plan. Our secondary PI, Dr. Guannan Liu, Dr. Fengxue Xin and Dr. Ziyi Yu helped us troubleshootour experimental design.

Xia Zhang and Changbin Gong are the teachers in charge of thelab we use in the Biochemical Engineering Experimental Teaching Center of NanjingTech University. Thanks to their support for our experimental implements and experimental materials.

Human Practices support

Thanks to professor Jinrong Liu, who suggested that we can determine the content of phenylethanol by HPLC.

Thanks to JunJie Zhao, the leader of Zhe Jiang Xin He Cheng company, who told us about the condition and main problem in the industry of phenethyl alcohol.

Thank NAU-China, IBOWU-China, LZU-China, HZAU-China, ZJU-China for collaboration with our team.

Other support

Wendong Zhang is under graduates who withdrew from our team midway. Although she failed to complete the competition, thank her for her hard work in the preliminary planning of our project.

Thanks to DreamWorks Studios of Nanjing Tech University for the equipment support during the presentation video recording.

Sponsors

Thanks to College of Biotechnology and Pharmaceutical Engineering of Nanjing Tech University for supporting us with registration fees of this competition.

Thanks to the 2011 College of Nanjing Tech University, Jiangsu Synergetic Innovation Center for Advanced Bio-Manufactureand for providing financial support to our team.

Other

Project history

We started brainstorming in March this year, providing initial ideas for the project. The specific tasks arrangement and schedule planning began in May. The wet-lab experiment started in June.

About the synthetic biology course

We are a team from NanjingTech University. Our school teaches a synthetic biology course in the form of elective course, which can be taken by juniors