Please note, during the course of our experiments and in compliance with the iGEM safety rules, we will not use or test any part of your project, or products produced from it, on humans (including members of the team) and any other multicellular organism. Also, we have not yet implemented any of our ideas into the real world, and will not release any organism or product derived from our project, so the topics discussed on this page are only proposed implementations.
- Sensitivity Tests
For these tests, we use E.Coli strain ATCC 25922 and its medium in a Petri dish. We added the bacteria medium all the way until the water level is slightly higher than the dyed cloth put in the middle of our dish. The ATCC 25922 was given us in a concentration of 1 * 108, so we had to dilute the solution for the bacteria have a concentration of 1*105.
To test for the efficiency, we use a mechanical pipette to capture 10 μL of solution for every millimeter. The solution was put in a centrifuge tube and deionized water was added to the 0.1 mL mark. After leaving the tube there for about 10 minutes, we tested its E. Coli concentration using test strips we bought on the internet. According to the test strips, an area concentration less than 5*103 is considered AgNP-effective. We measured the radius of the border at which the cloth is not effective for different combinations of solution, and then sent the results to our modeling team to calculate the most effective combinations.
- Starting Off With Masks
For our project this year, we decided to start off by modifying surgical masks. From the beginning of 2020 till now, Covid-19 has been spreading and showing us its impact on people’s livelihoods and health. It spreads mainly from person to person through respiratory droplets. This is why masks are vital in preventing the virus from spreading, and many studies have proven this to be true, and why we have decided to start our project off with masks.
The masks that have been dyed with ANTI-Virus will prevent the viruses and bacteria in the air from adhering to the masks and kill those that somehow find a way to stay on masks, all when retaining the mask’s electric discharge and polypropylene network. All of the cloth that we will be dyeing is cut out and folded in the same way the first layer of a surgical masks is. After the dyeing process and the cloth is dry, we will replace the first layer of cloth with our ANTI-Virus dyed cloth.