Team:MTU-CORK/Parts

Parts

Parts Collection

Registry Number Name Description Source
BBa_K4051016 A. Suillum Cld Chlorite dismutase sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0145 Genbank Accession: CP003153.1 AA sequence back translated to DNA via EMBOSS bactranseq Generic Histag used Generic Secretion Signal from Gaussian luciferase
BBa_K4051014 A. Suillum PCRA PCRA sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0149 Genbank Accession: CP003153.1
BBa_K4051017 A. Suillum PCRB PCRB sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0148 Genbank Accession: CP003153.1
BBa_K4051018 A. Suillum PCRC PCRC sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0147 Genbank Accession: CP003153.1
BBa_K4051019 A. Suillum PCRD PCRD sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0146 Genbank Accession: CP003153.1
BBa_K4051020 A. Suillum PCRABCD Entire perchlorate reductase island attached with stop codon and BioBrick in between. Previous sequences stitched together. BioBrick is the same that the Exeter iGem team used in 2018. BioBrick B0034 (BBa_B0034). Stop Codons randomly selected.


Parts with green fluorecent protein (gfp)

Registry Number Name Description Source
BBa_K4051021 A. Suillum Cld with GFP Chlorite dismutase sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0145 Genbank Accession: CP003153.1 AA sequence back translated to DNA via EMBOSS bactranseq Generic Histag used Generic Secretion Signal from Gaussian luciferase
BBa_K4051022 A. Suillum PCRA with GFP PCRA sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0149 Genbank Accession: CP003153.1
BBa_K4051023 A. Suillum PCRB with GFP PCRB sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0148 Genbank Accession: CP003153.1
BBa_K4051024 A. Suillum PCRC with GFP PCRC sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0147 Genbank Accession: CP003153.1
BBa_K4051025 A. Suillum PCRD with GFP PCRD sequence with added primer flanks, secretion signal and histag. Amino Acid (AA) Sequence translated, via benchling, from Dechloromonas (Azospira) suillum locus Dsui_0146 Genbank Accession: CP003153.1