Team:IISc-Bangalore/Parts
Parts
This page provides a quick summary of the basic and composite genetic parts constructed by us. These parts have been uploaded on the Registry of Standard Biological Parts, and detailed description of each of them may be found on the corresponding part pages on the Registry.
| Part Number | Part Class | Part Type | Name | Description |
|---|---|---|---|---|
| BBa_K3765000 | Basic | Tag | SpyTag002 | Forms an isopeptide bond with SpyCatcher002, used for anchoring two fusion proteins. |
| BBa_K3765001 | Basic | Coding | SpyCatcher002 | Forms an isopeptide bond with SpyTag002, used for anchoring two fusion proteins. |
| BBa_K3765002 | Basic | Tag | PelB Signal Peptide | Directs a protein to the periplasmic space in Gram-negative bacteria. |
| BBa_K3765003 | Basic | Tag | 10xHis Tag | Attached to recombinant proteins for purification by Ni-NTA Affinity Chromatography. |
| BBa_K3765004 | Basic | Coding | dCBD (Double Cellulose Binding Domain) | Binds to microcrystalline cellulose (MCC) via non-covalent interactions. |
| BBa_K3765005 | Basic | DNA | Glycine-Serine linker (GSGGS) | Flexible Gly-Ser linker to space out protein domains in a fusion protein. |
| BBa_K3765006 | Basic | DNA | Glycine-Serine linker (GSSGS) | Flexible Gly-Ser linker to space out protein domains in a fusion protein. |
| BBa_K3765007 | Basic | Tag | OpdA Signal Peptide | Native signal peptide from OpdA enzyme of Agrobacterium tumefaciens, directs a protein to the periplasmic space in Gram-negative bacteria. |
| BBa_K3765008 | Basic | Coding | OpdA | Organophosphate degrading enzyme from Agrobacterium tumefaciens. |
| BBa_K3765009 | Basic | DNA | 22 Amino Acid Linker | Long, flexible linker to space out protein domains in a fusion protein. |
| BBa_K3765010 | Basic | Coding | sfGFP (Superfolder GFP) | Robust variant of GFP which retains fluorescence characteristics even when fused to poorly folding proteins. |
| BBa_K3765011 | Basic | Plasmid | pETMCN-T7 | High copy number KanR vector for expressing protein-of-interest under T7 promoter. |
| BBa_K3765012 | Composite | Composite | PelB_Signal + 10xHis + SpyCatcher002 + dCBD | CDS of Construct C1; can be used for anchoring SpyTagged proteins onto a MCC matrix. |
| BBa_K3765013 | Composite | Composite | OpdA_Signal + OpdA + 22aaL + SpyTag002 + sfGFP + 10xHis | CDS of Construct C2; SpyTagged OpdA enzyme with native signal peptide and sfGFP as a reporter. |
| BBa_K3765014 | Composite | Composite | OpdA_Signal + OpdA + 22aaL + SpyTag002 + 10xHis | CDS of Construct C2(2.0); SpyTagged OpdA enzyme with native signal peptide. |
| BBa_K3765015 | Composite | Composite | OpdA + 22aaL + SpyTag002 + 10xHis | CDS of Construct C2(3.0); SpyTagged OpdA enzyme. |
| BBa_K3765016 | Composite | Translational Unit | T7 RBS + SpyCatcher002+dCBD Translational Unit of C1; | CDS of C1 under a strong RBS from T7 phage gene 10. |
| BBa_K3765017 | Composite | Translational Unit | T7 RBS + OpdA + SpyTag002 + sfGFP with OpdA signal peptide | Translational Unit of C2; CDS of C2 under a strong RBS from T7 phage gene 10. |
| BBa_K3765018 | Composite | Translational Unit | T7 RBS + OpdA + SpyTag002 with OpdA signal peptide | Translational Unit of C2(2.0); CDS of C2(2.0) under a strong RBS from T7 phage gene 10. |
| BBa_K3765019 | Composite | Translational Unit | T7 RBS + OpdA +SpyTag002 without OpdA signal peptide | Translational Unit of C2(3.0); CDS of C2(3.0) under a strong RBS from T7 phage gene 10. |
| BBa_K3765020 | Composite | Generator | SpyCatcher002 + dCBD under T7 promoter | C1 Protein Generator; for expression of C1 under strong T7 promoter and lac operator followed by strong T7 terminator. |
| BBa_K3765021 | Composite | Generator | OpdA + SpyTag002 + sfGFP with OpdA signal peptide under T7 promoter | C2 Protein Generator; for expression of C2 under strong T7 promoter and lac operator followed by strong T7 terminator. |
| BBa_K3765022 | Composite | Generator | OpdA + SpyTag002 with OpdA signal peptide under T7 promoter | C2(2.0) Protein Generator; for expression of C2(2.0) under strong T7 promoter and lac operator followed by strong T7 terminator. |
| BBa_K3765023 | Composite | Generator | OpdA + SpyTag002 without OpdA signal peptide under T7 promoter | C2(3.0) Protein Generator; for expression of C2(3.0) under strong T7 promoter and lac operator followed by strong T7 terminator. |