Team:Hong Kong HKU/Experiments

Experiments

Protocols

Here is a document for the protocols we used for our project, some of which are compiled from different sources after extensive research.

They include:
  • Standard PCR
  • Standard restriction digestion of biobricks
  • Standard ligation of biobricks
  • Chemical transformation
  • Lambda Red Protocol with pKD46
  • Natural transformation for cyanobacteria
  • O.D. measurement for cyanobacteria
  • Co-culture, growth conditions according to Liu et al.