Team:Gifu/Parts
iGEM Gifu Parts
Our basic parts are the coding sequence of Cas protein and sgRNA. There is Cas12a(LbCpf1) for Cas protein and sgRNA. For composite parts, we have promoter, RBS, affinity tag, and terminator in addition to the coding sequence.
Basic Parts
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K4031000 | coding | Lb cpf1(Cas12a) protein | 3684 |
| BBa_K4031001 | sgRNA | Cas12a T7 bacteriophage crRNA 1 | 41 |
| BBa_K4031002 | sgRNA | Cas12a T7 bacteriophage crRNA 2 | 41 |
| BBa_K4031003 | sgRNA | Cas12a T7 bacteriophage crRNA 3 | 41 |
| BBa_K4031004 | sgRNA | Cas12a HHV-6 crRNA 1 | 41 |
| BBa_K4031005 | sgRNA | Cas12a HHV-6 crRNA 2 | 41 |
| BBa_K4031006 | sgRNA | Cas12a HHV-6 crRNA 3 | 41 | BBa_K4031014 | tag | ×10 His Tag | 49 | BBa_K4031016 | tag | MBP Tag | 1101 |
Composite Parts
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K4031017 | composite | Lb cpf1(Cas12a) protein | 5323 |
| BBa_K4031008 | composite | Cas12a T7 bacteriophage crRNA 1 | 60 |
| BBa_K4031009 | composite | Cas12a T7 bacteriophage crRNA 2 | 60 |
| BBa_K4031010 | composite | Cas12a T7 bacteriophage crRNA 3 | 60 |
| BBa_K4031011 | composite | Cas12a HHV-6 crRNA 1 | 60 |
| BBa_K4031012 | composite | Cas12a HHV-6 crRNA 2 | 60 |
| BBa_K4031013 | composite | Cas12a HHV-6 crRNA 3 | 60 |