Team:Estonia TUIT/Parts
In this iGEM season, our team has added twelve coding sequences and one regulatory sequence to the Registry of Standard Biological Parts. The parts are described in the table below, further description can be found in the registry
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K4020000 | Coding | LexA binding domain - VP16 activation domain is a transcription factor. LexA Binding Domain is a sequence in the LexA protein, a transcriptional repressor of the SOS regulon in Escherichia coli which allows the binding of different regulatory molecules. VP16 is a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early genes. | 861 |
| BBa_K4020001 | Coding | LexA Binding Domain is a sequence in the LexA protein, a transcriptional repressor of the SOS regulon in Escherichia coli that allows the binding of different regulatory molecules. | 606 |
| BBa_K4020002 | Coding | SIC1 is a gene encoding a protein that inhibits cyclin-dependent kinase complexes and G1 to S phase progression in cell cycle, prevents premature S phase entry, and warrants genome integrity. A non-degradable variant of Sic1 due to the deletion of the N-terminus. | 582 |
| BBa_K4020003 | Coding | A highly sequence-specific cysteine protease. Its target substrate is the sequence ENLYFQS/G and it cleaves between Q and G/S, all the while requiring a Gly or Ser residue to be to be located in the vicinity of the cleavage site. | 762 |
| BBa_K4020004 | Regulatory | LexA is a synthetic promoter that contains four LexA boxes. LexA boxes are parts derived from the bacterial LexA promoter and contain two SOS motifs. The synthetic promoter has a core of the modified CYC1 promoter from Saccharomyces cerevisiae. | 353 |
| BBa_K4020005 | Coding | The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes. The residues composing the VP16ad/n subdomain are from 412 to 453. | 237 |
| BBa_K4020006 | Coding | The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes. The residues composing the VP16ad/c subdomain are from 454 to 490. | 237 |
| BBa_K4020007 | Coding | LexA Binding Domain (pBT3-N) SID (TEV site linker included) is a DNA sequence that encodes SID linker from SALSA protein, TEV protease recognition site, followed by LexA Binding Domain. | 696 |
| BBa_K4020008 | Coding | SID (TEV site linker included) VP16 activation domain is a sequence that includes SID linker from SALSA protein, TEV protease recognition site and VP-16 activation domain, which is a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early genes. | 327 |
| BBa_K4020009 | Coding | Prolyl peptidase is a serine protease that cleaves C-terminally of proline residues in peptides which are at least 30 amino acids long. | 2133 |
| BBa_K4020010 | Coding | The scavenger receptor cysteine-rich (SRCR) domain is a highly conserved domain of about 110 residues necessary for binding bacterial ligands. | 303 |
| BBa_K4020011 | Coding | SIDs (SRCR-interspersed domains) are roughly 20-amino-acid-long threonine-serine-proline-rich intrinsically disordered regions. | 93 |
| BBa_K4020012 | Coding | SID linker (TEV site included) is a sequence of modified SID from SALSA protein with an integrated TEV protease recognition site. | 93 |