Team:Estonia TUIT/Experiments
Protocols play a key role in uniformity of processes and the creation of successful products during experimental laboratory work. Protocols outlined here were provided to us by supervisors and sponsors and are the basis for all experiments mentioned in the Notebook.
The page includes the following protocols:
DNA polymerase chain reaction protocol
Restriction digestion protocol
Agarose gel for DNA separation
Extraction of genomic DNA from yeast cells
DNA purification from agarose gel
DNA concentration measurements
Ligation protocol, Golden Gate assembly
Bacterial transformation
Plasmid extraction from bacterial cells
Yeast transformation • Yeast colony PCR
LB and YPD media • Microscopy
Competitive growth assay
