Team:Estonia TUIT/Experiments


Protocols play a key role in uniformity of processes and the creation of successful products during experimental laboratory work. Protocols outlined here were provided to us by supervisors and sponsors and are the basis for all experiments mentioned in the Notebook.

The page includes the following protocols:

DNA polymerase chain reaction protocol

Restriction digestion protocol

Agarose gel for DNA separation

Extraction of genomic DNA from yeast cells

DNA purification from agarose gel

DNA concentration measurements

Ligation protocol, Golden Gate assembly

Bacterial transformation

Plasmid extraction from bacterial cells

Yeast transformation • Yeast colony PCR

LB and YPD media • Microscopy

Competitive growth assay

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