Team:Crete/Notebook


Lab Notebook

WEEK 1 (19-25/07)
  • Receiving of 3 plasmids containing the 3 s1 spike sequences and 1 plasmid containing mCherry and the transmembrane domain.
  • Lab sorting
  • Lab safety training
WEEK 2 (26/07-1/08)
  • Golden Gate Cloning
  • Start of an E.coli DH10B culture
WEEK 3 (2-8/08)
  • Transformation of E.coli DH10B
  • Selection of bacteria transformed with the recombined vector
  • Culture of selected bacteria
WEEK 4 (9-15/08)
  • Lab Closed
WEEK 5 (16-22/08)
  • Lab closed
WEEK 6 (23-29/08)
  • Isolation of plasmid DNA from recombined E.coili cultures
  • Amplification via PCR
WEEK 7 (30/08-5/09)
  • Issue at transferring plasmid DNA from pBlueScript to pICSL86922
WEEK 8 (6-12/09)
  • Transformation of Agrobacteria Tumefaciens with plCSL86922
  • Culture of Agrobacteria
  • Gel electrophoresis of the plasmid
WEEK 9 (13-19/09)
  • Selection of transformed Agrobacteria cultures
  • Culture of selected bacteria
WEEK 10 (20-26/09)
  • Agroinfiltration of plant leaves with transformed Agrobacteria
  • Confocal microscopy of plant leaves
WEEK 11 (27/09-3/10)
  • Repeat of the confocal microscopy session
  • Further Agroinfiltration of plants to create a sufficient quantity of the edible vaccine