Team:CHINA-FAFU/Parts

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Parts

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Overview

We have built 11 Basic Parts and 5 Composite Parts in total. Five Composite Parts are used to express APX1, DHAR, UGP, and VTC2 respectively.

Basic Parts

Name	Type	Description
BBa_K3812000	Coding	APX1
BBa_K3812001	Coding	DHAR
BBa_K3812002	Coding	UGP
BBa_K3812003	Coding	VTC2
BBa_K3812004	Reporter	sfGFP
BBa_K3812005	Promoter	Paox1
BBa_K3812006	Terminator	CYC1
BBa_K3812011	RBS	BW25113/mRFP1_RBS11
BBa_K3812012	Tag	6xHis-tag
BBa_K3812013	Terminator	L3S3P21
BBa_K3812014	Plasmid	pSB1C3

Composite Parts

Name	Type	Description
BBa_K3812007	Plasmid	Coding Ascorbate peroxidase
BBa_K3812008	Plasmid	Coding Dehydroascorbate reductase
BBa_K3812009	Plasmid	Coding UGPase
BBa_K3812010	Plasmid	Coding GDP-L-Galactose Phosphorylase
BBa_K3812016	Plasmid	Express DHAR more efficiently

Part Collection

In order to better optimize BBa_K3812008 so that DHAR can be better expressed, with the help of Ailurus^TM, we connect BBa_K3812001 to 2500 expression vectors and introduce them into DH5α for expression. Finally, we screened out the best expression system for DHAR-BBa_K3812016.