Difference between revisions of "Team:Xiamen City/Partnership"

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            <!--Modeling-->
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<div class="column full_size">
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            <!--Implementation-->
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<h1>Partnership</h1>
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            <li class="item">
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<h3>Gold Medal Criterion #5</h3>
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                <a href="https://2021.igem.org/Team:Xiamen_City/Implementation">Implementation</a>
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<p> Collaborate throughout the year with at least one other 2021 iGEM team on a set of shared objectives related to both of your projects. This partnership should go beyond a Silver medal collaboration.
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            </li>
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<br><br>
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
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            <!--Human Practice-->
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            <!--Parts-->
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                                Collection</a>
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                    </ul>
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                        </li>
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                            <a href="https://2021.igem.org/Team:Xiamen_City/Experiments">Experiments</a>
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    <!--内容-->
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        <div class="content-middle">
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            <section class="article p-t-30 p-b-54">
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                <h1 class="content-header">Partnership</h1>
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                <img src="https://static.igem.org/mediawiki/2021/e/ed/T--Xiamen_City--img_partnership_1.jpg" alt="" style="width: 80%">
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                <section>
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                    <h1 class="title">Background of Partnership</h1>
 +
                    <p>VIPatent (Shanghai_Metro) is also a 2021 iGEM high school team. They focuses on developing a quick,
 +
                        effective and affordable strain patent protection solution, whose mechanism is designed based on the
 +
                        type IV secretion system (T6SS) of Gram-negative bacteria which would take effects on both
 +
                        eukaryotic cells and bacterial cells. Since our CRISPR-Cas 9-edited wine yeast is of huge commercial
 +
                        values and originally-designed, we consider that it is necessary to protect its patent. After an
 +
                        in-depth communication with the team VIPatent, we found out that their idea would be feasible. Thus,
 +
                        we decided to develop partnership with this wonderful team. We designed a specific partnered
 +
                        experiment plan with team VIPatent together, which is shown as follows:</p>
 +
 +
                    <section>
 +
                        <h2 class="title2">Overview of VIPatent Project (Strain Patent Protection Principles)</h2>
 +
                        <ul class="dot">
 +
                            <li><p>Originally-designed plasmids are transformed to E. Coli (DH5a) to realize patent
 +
                                protection;</p></li>
 +
                            <li><p>Options of originally-designed plasmids could be Pus232-ike2-tke2 or
 +
                                Pus232-ike4-tke4;</p>
 +
                            </li>
 +
                            <li><p>Pus232 is extracted from E.Coli; gene ike and tke are extracted from Pseudomonas
 +
                                putida;</p>
 +
                            </li>
 +
                            <li><p>Gene tke secretes toxin while gene ike produces protein immune to that toxin;</p></li>
 +
                            <li><p>The promoter of gene ike is inducible, indicating that only with the induction of a
 +
                                specific
 +
                                ligand, will gene ike be expressed;</p></li>
 +
                            <li><p>The promoter of gene tke is constitutive, indicating that its toxic protein expresses at
 +
                                any
 +
                                time.</p></li>
 +
                        </ul>
 +
                    </section>
 +
 +
                    <section>
 +
                        <h2 class="title2">How to Protect the Patent of our Gene-edited Yeast through Partnership?</h2>
 +
                        <ul>
 +
                            <li><p>Extract gene ike-2-tke2 or ike4-tke4 from originally-designed plasmids;</p></li>
 +
                            <li><p>Amplify the extracted genes by PCR;</p></li>
 +
                            <li><p>Produce homologous recombination template by CRISPR, and then integrate the extracted
 +
                                genes to the genome of our yeast ; (Since the effector of gene tke and immune factor of
 +
                                gene ike are commonly used, it is unnecessary to re-design them)</p></li>
 +
                            <li><p>After the gene editing, put our yeast in a certain ligand to realize patent
 +
                                protection.</p></li>
 +
                        </ul>
 +
                    </section>
 +
 +
                    <section>
 +
                        <h2 class="title2">Mutual Benefits for us and Team VIPatent </h2>
 +
                        <ul>
 +
                            <li><p>We will acquire useful patent protection tools for our gene-edited wine yeast, with which
 +
                                we may and further values to in commercialization;</p></li>
 +
                            <li><p>Commercialization opportunities for team VI Patent, if the partnership succeeds between
 +
                                us and VIPatent, this model could be proliferated fast to other iGEM teams, enhancing the
 +
                                impact of team VIpatent;</p></li>
 +
                            <li><p>Demonstration of iGEM spirit-creating a mutually-beneficial, open, and collaborated
 +
                                platform for synthetic biology and related projects.</p></li>
 +
                        </ul>
 +
                    </section>
 +
 +
                    <section>
 +
                        <h2 class="title2"></h2>
 +
                        <ul>
 +
                            <li><p></p></li>
 +
                        </ul>
 +
                    </section>
 +
                </section>
 +
 +
                <section>
 +
                    <h1 class="title"></h1>
 +
                    <p></p>
 +
                </section>
 +
            </section>
 +
        </div>
 +
    </div>
 +
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content">
 +
            <h2 style="font-size: 28px; margin-bottom: 12px !important;">Contact Info</h2>
 +
            <img src="https://static.igem.org/mediawiki/2021/b/ba/T--Xiamen_City--qrcode.jpg" alt="" style="width: 200px">
 +
            <div style="margin-top: 12px"><i><span>WeChat Official Account:</span><span style="color: #00177a"> iGEM PecTeast</span></i>
 +
            </div>
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</div>
 
</html>
 
</html>

Revision as of 09:52, 17 October 2021

Partnership

Background of Partnership

VIPatent (Shanghai_Metro) is also a 2021 iGEM high school team. They focuses on developing a quick, effective and affordable strain patent protection solution, whose mechanism is designed based on the type IV secretion system (T6SS) of Gram-negative bacteria which would take effects on both eukaryotic cells and bacterial cells. Since our CRISPR-Cas 9-edited wine yeast is of huge commercial values and originally-designed, we consider that it is necessary to protect its patent. After an in-depth communication with the team VIPatent, we found out that their idea would be feasible. Thus, we decided to develop partnership with this wonderful team. We designed a specific partnered experiment plan with team VIPatent together, which is shown as follows:

Overview of VIPatent Project (Strain Patent Protection Principles)

  • Originally-designed plasmids are transformed to E. Coli (DH5a) to realize patent protection;

  • Options of originally-designed plasmids could be Pus232-ike2-tke2 or Pus232-ike4-tke4;

  • Pus232 is extracted from E.Coli; gene ike and tke are extracted from Pseudomonas putida;

  • Gene tke secretes toxin while gene ike produces protein immune to that toxin;

  • The promoter of gene ike is inducible, indicating that only with the induction of a specific ligand, will gene ike be expressed;

  • The promoter of gene tke is constitutive, indicating that its toxic protein expresses at any time.

How to Protect the Patent of our Gene-edited Yeast through Partnership?

  • Extract gene ike-2-tke2 or ike4-tke4 from originally-designed plasmids;

  • Amplify the extracted genes by PCR;

  • Produce homologous recombination template by CRISPR, and then integrate the extracted genes to the genome of our yeast ; (Since the effector of gene tke and immune factor of gene ike are commonly used, it is unnecessary to re-design them)

  • After the gene editing, put our yeast in a certain ligand to realize patent protection.

Mutual Benefits for us and Team VIPatent

  • We will acquire useful patent protection tools for our gene-edited wine yeast, with which we may and further values to in commercialization;

  • Commercialization opportunities for team VI Patent, if the partnership succeeds between us and VIPatent, this model could be proliferated fast to other iGEM teams, enhancing the impact of team VIpatent;

  • Demonstration of iGEM spirit-creating a mutually-beneficial, open, and collaborated platform for synthetic biology and related projects.

Contact Info

WeChat Official Account: iGEM PecTeast