We introduced CRISPR technology and PCR technology to our community in a PowerPoint presentation and a recorded video. The majority of our educational audience were children. Therefore, we streamlined the PowerPoint and video instruction to make it easier to understand. Initially, we included a detailed description of CRISPR and PCR technologies in the PowerPoint. All of the materials mentioned in the PPT are told with vivid cartoon and pictures.
In these material, we showed children process of yeast decomposition and the importance of multifunctional yeast in the winemaking. We also explained gene-editing technology in the simplest terms. We gave the children some knowledge about the structure of plant roots, stems and leaves. Dividing the children into groups, we organized the students to work together to put together the root and leaf structure of plants, which brought them a lot of joy.
We have also set up our WeChat official account as an important communication channel with the public. Initially, the account functions as a recorder of what we have done. As our followers increased rapidly, we found it a great means to educate the public about gene-editing technique. We have published 7 articles and some of them have been read by over 1000 people.
As we have rent an office to work together in a crowded office building, we took advantage of great human traffic in the morning and evening peak. We designed a roll up banner and placed it in front of our office, and took turns to hand out brochures with our official account's QR code of our official account. To avoid people's aversion, we also handed out free masks with our cute logo on it. By doing this, our official account obtained a great number of new followers.
In order to enlarge our impact, we intend to make a MV for our team. We rewrote the lyric of STAY( from the Kid LAROI/Justin Bieber). We talked about our development procedures of designing multifunctional yeast and our future market entrance plan. We are still designing and making the final video. We plan to release the video on several popular platforms, such as YouTube, Bilibili and Weibo.
Here attached the lyric ( we recompose the lyrics).
In addition, to make the process of knowing synthetic biology intriguing, our team designs an aeroplane chess map. The rules are similar to the original ones. We make some changes in the layout of our map and the ‘ accident’ cards. Here attached our game.
DNA: (HEREDITY) The next round will have the same number of points as this round
RNA: (HEREDITY) Subtract 1 step per round
CRISPR-CAS9: Subtract the number of steps in the next round from the number of steps in the current round. Take the absolute value if the number of steps in the next round is less.
Vaccines: Immunize all effects（e.g. ｜6-5｜=1 or ｜5-6｜=1）
Saccharomyces Cerevisiae: (Fermentation) Double the number of points in the next round
Cell Division: Stepping on a bond piece will have a split function. When stepped on by other bonds won’t die, instead of going to the starting point. It can be split into 2 small bond, but both of them can only be counted as one.
Pectase: (CATALYSIS) Roll the dice twice in the next round, help to get to terminus faster.
Pectin: (VISCIDITY) Get stuck in place one round
Virus: Has the ability of attacking other players
one player can be designated to take one step back each turn
overtake a designated player
To further enhance our influence, we planned to contribute and publish our research paper on public education website Questioz, our abstract are followings:
During pandemic, transportation and trading has been greatly blocked . As commodity are deteriorating rapidly, fruit market has been greatly impacted. Many fruits were heavily stagnated and fruit farmers lost stable income. This study produces a new saccharomyces cerevisiae that might offers a solution to this problem.
After two rounds of previous research, interviewing several bioengineering and zymurgy experts, we found this problem can be solved indirectly in biological method. Researchers are looking forward to genetically-edit saccharomyces cerevisiae so it acquires pectinase’s function. This new yeast is expected to cut fruit wine production cost in order to lower wine industry’s barrier and finally increase fruit demand.
Using CRISPR-Cas9 technology, the heterologous gene expression process of endo-PGAA gene from Aspergillus Niger SC323 in wine yeast was introduced into phcas9-Nours plasmid containing Cas gene. Then, the pYES2 -- GRNA-HYg-MCS plasmid carrying sgRNA (anchoring HXK1) and the homologous arm for repair were introduced into the fruit wine yeast. Lastly, the transposers were obtained to verify whether endo-PGAA was replaced to the HXK1 gene site.
The process of verifying the ability of saccharomyces cerevisiae to produce pectinase are followings. Bromophenol blue medium is used to verify whether the integrated strain produced pectinase. The integrated strains are inoculated to 30°C incubator and cultured for 3 days. If we found that the integrated strains on bromophenol blue medium had obvious yellow circles, while the wild type strains did not, we can indicate that endo-PGAA gene has been successfully introduced into saccharomyces cerevisiae.
The experiment turned out to be successful. The final product can degrade pectin well and replace pectin in wine production process. Researchers are looking forward to commercializing it in the near future.
Here attached some question samples we used while we are interviewing with professors and passing-by.
What do you think of the recent wine market? What about fruit wines?
Is there any national policy (regulation) on the wine market now? (Recently)
Do you think wineries will buy our yeast?
How to deal with expired fruit wines?
Will it affect the stagnant fruits?
Is it costly to make fruit wine now?
What do you think about gene editing technology (are you willing to consume genetically edited food?)
Do you know the difference between fruit wine and other wines?
How often do you usually drink wine?
What do you think is the impact of using gene editing technology to reduce the price of fruit wines on the sales of fruit wines: good/bad/not much?