Difference between revisions of "Team:Shanghai City United/Implementation"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2021.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2021.igem.org/Judging/Awards"> award listed below</a>. </p>
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                    <a href="https://2021.igem.org/Team:Shanghai_City_United">Home</a>
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                    <a href="">Project</a>
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<h1>Proposed Implementation</h1>
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                        <ul>
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<h3>Silver Medal Criterion #4</h3>
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                                <a href="https://2021.igem.org/Team:Shanghai_City_United/Description"
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<p>Explain how you would implement your project in the real world.
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                                    class="sub-nav-74">Description</a>
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information. </p>
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                                    class="sub-nav-74">Results</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Proof_Of_Concept"
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                    <a href="#">Implementation</a>
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        <div class="sub-title">Implementation</div>
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        <div class="article-title black-font" style="line-height: 40px;">Feed Additives Make the Poultry Grain Feed
 +
            Powerful in Digestion</div>
 +
        <div class="article-content">Monogastric animals (such as poultry) have simple digestive tract. Their digestive
 +
            organs are small in volume and cannot secrete cellulase and hemicellulase. However, saliva contains a large
 +
            amount of a-amylase, which can hydrolyze the a-1,4 glucose in the starch and make it a chain, including
 +
            maltose, dextrin, and so on. In the small intestine, small intestinal mucosal epithelial cells can produce a
 +
            variety of enzymes, such as lactase, maltase, etc., which can hydrolyze lactose cellobiose, maltose,
 +
            isomaltose a-1,6 chain dextrin, powder, and so on. Therefore, monogastric animals are suitable for various
 +
            grains and other refined feeds.</div>
 +
        <div class="article-content">However, monogastric animals can not digest the xylan contained in grain feed,
 +
            which may lead to a series of digestion problems for monogastric animals. Poultry are typical monogastric
 +
            animals. Poultry breeding industry impact our daily life, thereafter, how to provide poultry breeders with
 +
            high-quality feed is a critical issue. To address this issue, we start our project, aiming at developing a
 +
            probiotic (Lactobacillus) containing xylanase to produce feed additives. The feed additives will be added to
 +
            liquid beverage tailored for poultry. </div>
 +
        <div class="article-content">The potential end users of our products should mainly be poultry feed producers and
 +
            poultry breeders. We consider about two major business channels: 1) We will cooperate with poultry feed
 +
            producers to produce our feed additives and the liquid beverage; 2) We will try to produce the feed
 +
            additives and the liquid beverage ourselves and then sell them to poultry breeders. </div>
 +
        <div class="article-title black-font">Disign Concept of Our Product</div>
 +
        <div class="img-wrap">
 +
            <img src="https://static.igem.org/mediawiki/2021/d/d9/T--Shanghai_City_United--implementation01.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">Our project is to design an edible "drink" for monogastric animals, main poultry
 +
            through biosynthesis technology. </div>
 +
        <div class="article-content">The core product will be a probiotic that can produce xylanase. In this project, we
 +
            designed to construct the target plasmids through PCR amplifying genes, electrophoresis appraisal, and
 +
            ligation experiments, then we transformed them into E. coli and Lactobacillus reuteri for further
 +
            performance analysis.</div>
 +
        <div class="article-content">Compared to traditional feed, our drink contains probiotics that could help poultry
 +
            digest xylan, thus increasing feed efficiency. In this way, not only can the time and economic cost of
 +
            feeding be saved, but also the gastrointestinal tract of the animal can be protected and thus the disease
 +
            rate can be reduced.</div>
 +
        <div class="article-content">Currently, we have obtained the engineered L. returei which carries the plasmind
 +
            pSIP403-PUS-xyn AM and is verified to have well enzyme activity. To sum up, our product design is applicable
 +
            and promising.</div>
 +
        <div class="article-title black-font" style="line-height: 40px;">Safety Concerns and Challenges Confronted with
 +
            Our Implementation </div>
 +
        <div class="article-content">1) Market response towards engineered probiotic products</div>
 +
        <div class="article-content">· In reality, lots of people hold biases towards GMO products, especially about
 +
            their safety. Thus, our products may also be faced with the same challenge. Will our products cause harm to
 +
            the poultry’s health, especially to the digestion system? </div>
 +
        <div class="article-content">· To tackle this issue, further safety tests regarding our probiotic
 +
            (Lactobacillus) should be made. </div>
 +
        <div class="article-content">· Meanwhile, we need to make a series of safety education activities to inform our
 +
            potential clients of the GMO techniques, especially about their safety issues. </div>
 +
        <div class="article-content">2) Government regulations on our engineered probiotic products</div>
 +
        <div class="article-content">GMO products are strictly administrated in China, thus the commercialization of our
 +
            products may be quite challenging due to relevant policies.</div>
 +
        <div class="article-content">· To overcome this issue, we need to study the administration policies very clearly
 +
            and follow every step strictly. </div>
 +
        <div class="article-content">· Meanwhile, we should consider about consulting patent protection experts, legal
 +
            experts and experienced GMO experts to lower the possible legal risk. </div>
 +
        <div class="article-content">3) Effectiveness of our engineered probiotic products</div>
 +
        <div class="article-content">According to our design, the feed additives and liquid beverage will take effect
 +
            within poultry’s digestion system. However, since it is very difficult for xylanase to be inactivated, it is
 +
            possible that our product me be ineffective in practice. </div>
 +
        <div class="article-content">· To overcome this challenge, we must be very cautious in further experiments to
 +
            test the effectiveness of our products. (SURELY within the scope of iGEM, NO in vivo experiments are to be
 +
            done)</div>
 +
    </div>
 +
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            <div class="footer-contact">Contact Info.</div>
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            <p class="margin-bottom-10">WeChat Official Account:<i>谷饲嘉Grain Fed Plus</i></p>
 +
            <p>Email：<i>xxkk5905@gmail.com</i></p>
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Latest revision as of 13:43, 19 October 2021

Shanghai_City_United

Implementation
Feed Additives Make the Poultry Grain Feed Powerful in Digestion
Monogastric animals (such as poultry) have simple digestive tract. Their digestive organs are small in volume and cannot secrete cellulase and hemicellulase. However, saliva contains a large amount of a-amylase, which can hydrolyze the a-1,4 glucose in the starch and make it a chain, including maltose, dextrin, and so on. In the small intestine, small intestinal mucosal epithelial cells can produce a variety of enzymes, such as lactase, maltase, etc., which can hydrolyze lactose cellobiose, maltose, isomaltose a-1,6 chain dextrin, powder, and so on. Therefore, monogastric animals are suitable for various grains and other refined feeds.
However, monogastric animals can not digest the xylan contained in grain feed, which may lead to a series of digestion problems for monogastric animals. Poultry are typical monogastric animals. Poultry breeding industry impact our daily life, thereafter, how to provide poultry breeders with high-quality feed is a critical issue. To address this issue, we start our project, aiming at developing a probiotic (Lactobacillus) containing xylanase to produce feed additives. The feed additives will be added to liquid beverage tailored for poultry.
The potential end users of our products should mainly be poultry feed producers and poultry breeders. We consider about two major business channels: 1) We will cooperate with poultry feed producers to produce our feed additives and the liquid beverage; 2) We will try to produce the feed additives and the liquid beverage ourselves and then sell them to poultry breeders.
Disign Concept of Our Product
Our project is to design an edible "drink" for monogastric animals, main poultry through biosynthesis technology.
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct the target plasmids through PCR amplifying genes, electrophoresis appraisal, and ligation experiments, then we transformed them into E. coli and Lactobacillus reuteri for further performance analysis.
Compared to traditional feed, our drink contains probiotics that could help poultry digest xylan, thus increasing feed efficiency. In this way, not only can the time and economic cost of feeding be saved, but also the gastrointestinal tract of the animal can be protected and thus the disease rate can be reduced.
Currently, we have obtained the engineered L. returei which carries the plasmind pSIP403-PUS-xyn AM and is verified to have well enzyme activity. To sum up, our product design is applicable and promising.
Safety Concerns and Challenges Confronted with Our Implementation
1) Market response towards engineered probiotic products
· In reality, lots of people hold biases towards GMO products, especially about their safety. Thus, our products may also be faced with the same challenge. Will our products cause harm to the poultry’s health, especially to the digestion system?
· To tackle this issue, further safety tests regarding our probiotic (Lactobacillus) should be made.
· Meanwhile, we need to make a series of safety education activities to inform our potential clients of the GMO techniques, especially about their safety issues.
2) Government regulations on our engineered probiotic products
GMO products are strictly administrated in China, thus the commercialization of our products may be quite challenging due to relevant policies.
· To overcome this issue, we need to study the administration policies very clearly and follow every step strictly.
· Meanwhile, we should consider about consulting patent protection experts, legal experts and experienced GMO experts to lower the possible legal risk.
3) Effectiveness of our engineered probiotic products
According to our design, the feed additives and liquid beverage will take effect within poultry’s digestion system. However, since it is very difficult for xylanase to be inactivated, it is possible that our product me be ineffective in practice.
· To overcome this challenge, we must be very cautious in further experiments to test the effectiveness of our products. (SURELY within the scope of iGEM, NO in vivo experiments are to be done)
Contact Info.

WeChat Official Account:谷饲嘉Grain Fed Plus

Email：xxkk5905@gmail.com