Team:Shanghai City United/Contribution

Shanghai_City_United

Contribution

BBa_K4093004

== Profile ==

Name: pMD19-T-xynA
Base Pairs: 3346bp
Origin: Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed

== Usage and Biology ==

Feed grains are whole grains such as corn, wheat and barley used in the feeding of livestock and poultry. At present, corn is the main fodder in the feed industry. Due to the increasing shortage of raw materials and the rising price, the development of the feed industry has been greatly limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain, and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat, cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP), which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore, we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed absorption efficiency.

Figure1. Demonstration of Partnership

== Construct design ==

The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a expressing xylanase gene plasimd.

Figure 2. Schematic map of expression module of xynA .

BBa_K4093005

== Profile ==

Name: PUS-xynA
Base Pairs: 936bp
Origin: lactic acid bacteria, Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed

== Construct design ==

The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a expressing xylanase gene module.

Figure 2. Schematic map of expression module of xynA and PUS.

BBa_K4093006

== Profile ==

Name: pSIP403-PUS-xyn A
Base Pairs: 8354bp
Origin: E. coli , Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed

== Construct design ==

The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a plasmid expressing xylanase gene, then we transformed them into Escherichia coli and Lactobacillus reuteri for further performance analysis.

Figure 2. Schematic map of expression system of xynA plasmids.