Feed grains are whole grains such as corn, wheat and barley used
in the feeding of
livestock and poultry
◇ Barley is a grain with high energy and protein used for feed of ruminate
livestock, such as cattle. It can be used in growing and finishing diets or used in blends or feed
mixtures.
◇ Feed wheat is wheat that didn't reach standards needed for milling due to disease, drought
or sprouting. This feed-grade wheat is a palatable, digestible energy source for cattle, pigs and
poultry.
◇ Yellow corn is a major source of starch and is the best suited for processing and feedstock
for cattle and poultry and is available in bulk vessel, truck, rail or barge.
Team:Shanghai City United/Description
PROJECT DESCRIPTION
Inspiration
At present, corn is the main fodder in the feed
industry. Due to the increasing
shortage of raw materials and the rising price, the development of the feed industry has been greatly
limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain,
and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat,
cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP),
which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients
in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore,
we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed
absorption efficiency.
Grain-Fed+ Project
Our project is to design an edible "drink" for
monogastric
animals, mainly poultry
through biosynthesis technology.
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct the target plasmids through PCR amplifing genes, electrophoresis appraisal, and ligation experiements, then we transformed them into E. coli and Lactobacillus reuteri for further performance anaylisis.
Compared to traditional feed, our drink contains the probiotics that could help poultry digest xylan, thus increasing feed efficiency. In this way, not only can the time and economic cost of feeding be saved, but also the gastrointestinal tract of the animal can be protected and thus the disease rate can be reduced.
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct the target plasmids through PCR amplifing genes, electrophoresis appraisal, and ligation experiements, then we transformed them into E. coli and Lactobacillus reuteri for further performance anaylisis.
Compared to traditional feed, our drink contains the probiotics that could help poultry digest xylan, thus increasing feed efficiency. In this way, not only can the time and economic cost of feeding be saved, but also the gastrointestinal tract of the animal can be protected and thus the disease rate can be reduced.
Reference
1. Beasley S S,Takala T M， Reunanen J， Apajalahti J， Saris P
E.2004.Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and
intestine. Poult Science，83(1)：45-48;
2. De Vos W M.1999.Gene expression systems for lactic acid bacteria. Current
Opinion in Microbiology，2(3)：289-295;
3. Silversides F G， Scott T A， Korver D R，Afsharmanesh M，Hruby M.2006. A study on
the interaction of xylanase and phytase enzymes in wheat-based diets fed to commercial white and brown egg
laying hens.Poultry Science，85(2)：297-305;
4. 崔罗生，祝茂生，徐顺清，朱辉，梁运祥，张忠明.2009.黑曲霉木聚糖酶基因(xyn A)
在大肠杆菌中的表达及酶学分析.华中农业大学学报，28(1)：48-53；
5. 李慧.2010. 蛋白酶和木聚糖酶对肉鸡生长性能，消化机能及血液指标的影响.[研究生学位论文].杨凌：西北农林科技大学；
6. https://wenku.baidu.com/view/bb1a6d76590216fc700abb68a98271fe910eafb9.html