Team:William and Mary/Parts
Parts
Basic Parts
| Part in Registry | Part Type | Description | Length (bp) |
| BBa_B0034 | RBS | Elowitz RBS | 18 |
| BBa_B1006 | Terminator | Terminator (artificial, large, %T~>90) | 39 |
| BBa_K2680553 | DNA | UNS 1 | 40 |
| BBa_K2680554 | DNA | UNS 10 | 40 |
| BBa_R0082 | Promoter | ompC promoter | 108 |
| BBa_K2411002 | Terminator | T500 | 30 |
| BBa_K3773003 | CDS | Codon-optimized sfGFP | 759 |
| BBa_K3773006 | Promoter | clpB promoter | 390 |
| BBa_K3773007 | RBS | Native clpB RBS | 9 |
| BBa_K3773008 | RBS | Ceroni RBS-containing region | 32 |
| BBa_K3773009 | Promoter + RBS | dnaKJ operon promoter/RBS | 179 |
| BBa_K3773010 | Promoter | lon promoter | 253 |
| BBa_K3773011 | Promoter | hslVU promoter | 90 |
| BBa_K3773012 | Promoter | P70a (sequence from Marshall et al., 2019) | 67 |
| BBa_K3773013 | DNA | Spinach2 aptamer | 95 |
| BBa_K3773014 | CDS | sfGFP for use in vitro | 717 |
| BBa_K3773015 | RBS | RBS-containing region following P70a | 35 |
| BBa_K3773016 | Promoter + RBS | ompC promoter + RBS from Schmidl et al. | 142 |
| BBa_K3773017 | Promoter | flup1 promoter | 81 |
| BBa_K3773018 | Promoter | flup2 promoter | 81 |
| BBa_K3773019 | Promoter | ftsLp2 promoter | 85 |
| BBa_K3773020 | DNA | F30-Broccoli | 105 |
| BBa_K3773021 | Promoter | P70a (sequence from Alam et al., 2017) | 55 |
Composite Parts
| Circuit #(WM21_0...) | Part in Registry | Part Nickname | Description | Length (bp) |
| 11 | BBa_K3773511 | TX sensor | Circuit to report transcription in vivo | 259 |
| 12 | BBa_K3773512 | TX sensor (alt) | Circuit to report transcription in vivo (alternate design) | 279 |
| 13* | BBa_K3773513 | TL sensor (alt) | Circuit to report translation in vivo (alternate design) | 931 |
| 14 | BBa_K3773514 | Phosphorylation sensor | Circuit to report phosphorylation in vivo | 931 |
| 15* | BBa_K3773515 | Phosphorylation sensor (alt) | Circuit to report phosphorylation in vivo (alternate design) | 1112 |
| 16* | BBa_K3773516 | TL sensor | Circuit to report translation in vivo | 957 |
| 17* | BBa_K3773517 | clpB sensor** | Circuit to report PclpB expression in vivo | 1272 |
| 18* | BBa_K3773518 | clpB sensor (alt) | Circuit to report PclpB expression in vivo (alternate design) | 1281 |
| 19 | BBa_K3773519 | flup1 sensor | Circuit to report flup1 expression in vivo | 977 |
| 20 | BBa_K3773520 | flup2 sensor | Circuit to report flup2 expression in vivo | 977 |
| 21* | BBa_K3773521 | dnaKJ sensor | Circuit to report PdnaKJ expression in vivo | 1057 |
| 22 | BBa_K3773522 | ftsLp2 sensor | Circuit to report ftsLp2 expression in vivo | 981 |
| 23* | BBa_K3773523 | lon sensor | Circuit to report Plon expression in vivo | 1057 |
| 24* | BBa_K3773524 | hslVU sensor | Circuit to report PhslVU expression in vivo | 986 |
| 25 | BBa_K3773525 | CF TX sensor*** | Circuit to report transcription in vitro | 284 |
| 26 | BBa_K3773526 | CF TL sensor | Circuit to report translation in vitro | 929 |
| 27 | BBa_K3773527 | CF lon sensor | Circuit to report Plon expression in vitro | 1098 |
| 28 | BBa_K3773528 | CF clpB sensor | Circuit to report PclpB expression in vitro | 1235 |
*This part was successfully sequenced by Epoch Life Science and sequence-confirmed using Benchling tools.
**Italics in nicknames are not accurate on the Registry due to length limitations.
***CF stands for “cell-free” and is used to allow for a shorter nickname in the Registry.