Team:William and Mary/Parts



Parts

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Basic Parts

Part in Registry Part Type Description Length (bp)
BBa_B0034 RBS Elowitz RBS 18
BBa_B1006 Terminator Terminator (artificial, large, %T~>90) 39
BBa_K2680553 DNA UNS 1 40
BBa_K2680554 DNA UNS 10 40
BBa_R0082 Promoter ompC promoter 108
BBa_K2411002 Terminator T500 30
BBa_K3773003 CDS Codon-optimized sfGFP 759
BBa_K3773006 Promoter clpB promoter  390
BBa_K3773007 RBS Native clpB RBS 9
BBa_K3773008 RBS Ceroni RBS-containing region 32
BBa_K3773009 Promoter + RBS dnaKJ operon promoter/RBS 179
BBa_K3773010 Promoter lon promoter 253
BBa_K3773011 Promoter hslVU promoter 90
BBa_K3773012 Promoter P70a (sequence from Marshall et al., 2019) 67
BBa_K3773013 DNA Spinach2 aptamer 95
BBa_K3773014 CDS sfGFP for use in vitro 717
BBa_K3773015 RBS RBS-containing region following P70a 35
BBa_K3773016 Promoter + RBS ompC promoter + RBS from Schmidl et al. 142
BBa_K3773017 Promoter flup1 promoter 81
BBa_K3773018 Promoter flup2 promoter 81
BBa_K3773019 Promoter ftsLp2 promoter 85
BBa_K3773020 DNA F30-Broccoli 105
BBa_K3773021 Promoter P70a (sequence from Alam et al., 2017) 55

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Composite Parts

Circuit #(WM21_0...) Part in Registry Part Nickname Description Length (bp)
11 BBa_K3773511 TX sensor Circuit to report transcription in vivo 259
12 BBa_K3773512 TX sensor (alt) Circuit to report transcription in vivo (alternate design) 279
13* BBa_K3773513 TL sensor (alt) Circuit to report translation in vivo (alternate design) 931
14 BBa_K3773514 Phosphorylation sensor Circuit to report phosphorylation in vivo 931
15* BBa_K3773515 Phosphorylation sensor (alt) Circuit to report phosphorylation in vivo (alternate design) 1112
16* BBa_K3773516 TL sensor Circuit to report translation in vivo 957
17* BBa_K3773517 clpB sensor** Circuit to report PclpB expression in vivo 1272
18* BBa_K3773518 clpB sensor (alt) Circuit to report PclpB expression in vivo (alternate design) 1281
19 BBa_K3773519 flup1 sensor Circuit to report flup1 expression in vivo 977
20 BBa_K3773520 flup2 sensor Circuit to report flup2 expression in vivo 977
21* BBa_K3773521 dnaKJ sensor Circuit to report PdnaKJ expression in vivo 1057
22 BBa_K3773522 ftsLp2 sensor Circuit to report ftsLp2 expression in vivo 981
23* BBa_K3773523 lon sensor Circuit to report Plon expression in vivo 1057
24* BBa_K3773524 hslVU sensor Circuit to report PhslVU expression in vivo 986
25 BBa_K3773525 CF TX sensor*** Circuit to report transcription in vitro 284
26 BBa_K3773526 CF TL sensor Circuit to report translation in vitro 929
27 BBa_K3773527 CF lon sensor Circuit to report Plon expression in vitro 1098
28 BBa_K3773528 CF clpB sensor Circuit to report PclpB expression in vitro 1235


*This part was successfully sequenced by Epoch Life Science and sequence-confirmed using Benchling tools.

**Italics in nicknames are not accurate on the Registry due to length limitations.

***CF stands for “cell-free” and is used to allow for a shorter nickname in the Registry.