One of the fundamental pillars of ARIA is to advocate for open science. This is why all the material we have developed is public and accessible and may be used by future iGEM editions.
To contribute to the open science movement, we have guided all the processes that are reflected in our Wiki step by step. In this way, we wanted our project to also serve as a starting piece for others. For example, in the section dedicated to our models, an explanatory guide is included on how to make the mathematical model. On the other hand, we have a guide on how to build the biosensors and another on how the process of testing them is carried out. In addition, the protocols have not been made solely as a repository in which technical steps are detailed, but a particular order has been set to facilitate the replication of our experiments for other groups.
All the specifications of the constructs and tools that have been designed for our biological biosensors are found in the Parts section and their operation is detailed in Design. A new part collection has been created from scratch thinking of its usefulness for future groups of iGEMs who want to use CRISPR-Cas for diagnostics, it is available here.
Regarding the whole software section, we have made a public repository available for everyone in GitHub “team”. Inside, all the codes used for the material shown in the wiki can be found in user-friendly formats. Essentially, multiple python scripts and notebooks are provided with comments and text sections that guide the user through the whole pipeline so that results can be replicated.
Our software is under a general Affero GPL 3.0 license, which in simple terms means that is entirely open access and can be used by anyone, but their findings must be also open access even for service applications.
In addition, ARIA encourages everyone interested in the topic to use our tools to create more advanced systems that could be used for improving the antibiotic resistance paradigm we are (and will be) facing.
Adding information to an existing part: BBa_K2927005
A team from the 2019 edition of iGEM, iGEM19_CCU_Taiwan, used the same nuclease we used in our experiments: the LbCas12a. To complete the information available in the Registry of Standard Biological Parts, we have added data from our experiments in a new section called UPF Barcelona ARIA Team experimental application.
Useful fast tips for future iGEMs
We know that starting a project from scratch is not easy, so we have decided to develop materials that can be useful for future iGEM teams. We have based the document on those details that we would have liked to know before starting our project and that can facilitate the day to day of the contestants.
- WIKI TIPS: it is a document that gives tips for starting the programming of the Wiki, and specifically in reference to exclusive details of the iGEM server.