Team:Thessaly/Contribution




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Overview

As iGEM Thessaly 2021, focusing on certain aspects to our project constitutes a vital factor for helping other iGEM teams find synthetic and applicable solutions. Our contribution consists of four aspects: Parts, Modeling, Engagement and Cloning.

PARTS

Two parts of the registry were characterized by adding new documentation from bibliography and/or our experiments. Trying to strengthen the project design we chose to characterize Promoter FliC BBa_K2924016 which takes place in the SCFAs detection module, adding more documentation and testing more conditions to a part that was used by our team last year, in AMALTHEA 2020. We also made a literature documentation on BBa_K415202 CymR, which is the center of our probiotic Kill switch. For more, go to our Results Page.

MODELING

Every iGEM team has to support the work of the wet lab team. Complex systems must decisively rely on the use of modeling techniques. Modeling indicates a way of creating a synthetic biological system in vitro. We benefit from in vitro Biological systems after conducting simulations giving insights to Troubleshoot genetic circuit flaws. That’s why we developed the following guide.


Our Beginner’s Guide to SimBiology®


ENGAGEMENT

Based on our experience, it takes time and effort to manage and appropriately organise meaningful events that can bring iGEMers together, fulfill the competition's goals and be impactful on the issues we're tackling. Thereby, we believe our guide we'll be a great tool for new iGEMers to begin from and take public engagement to the next level.


Our Guide How to Organize a Mini Congress


CLONING

Cloning of basic parts and Building multiplex constructs are essential in a Synthetic Biologic Project that is based on the Design Build Test Learn (DBTL) cycle. AMALTHEA led us to build plenty of genetic circuits in order to test our modules. We believe we can contribute to the DBTL cycle by providing our knowledge on cloning.

Our Masterclass on Cloning Troubleshooting

igem.thessaly@gmail.com