Experiments

“When all else fails, read the instructions”

Here, you can find all the protocols used in our project. More detailed information about the execution of our project can be found on Notebook. Our results can be found on the Improvement, Results and Proof of Concept.

Overview of all the Experiments

All experiments executed during our project with their corresponding protocols can be found in the following booklet.

IBDetection Experiments

General Protocols

The general protocols we used are listed below.

Co-transformation
Gel electrophoresis
Gel extraction
Large culture & protein Expression
Making competent cells
Making TSS solution
Medium & pouring agar plates
Multi Site-directed mutagenesis
Nanodrop
PCR amplification & purification
Qiagen miniprep
Restriction & Ligation
Sequencing
Small culture
Stock solutions

Two component TtrS/R protocols

The specific protocol for the two component TtrS/R system is listed below.

GFP measuring

Acoustic Reporter Genes Protocols

The specific protocols for the Acoustic reporter genes are listed below.

ARG Imaging
ARG Purifying
SDS-PAGE

Improvement

The specific protocol for the part Improvement is listed below.

mRFP1 Fluorescence measurement

Team:TU-Eindhoven/Experiments

Overview of all the Experiments

General Protocols

Two component TtrS/R protocols

Acoustic Reporter Genes Protocols

Improvement