Team:Sydney Australia/Parts

Overview

The following is a table of our basic and composite parts. Our composite parts are our Babushka Blocks, which include the eight gene clusters and the two fliK landing pads.

Basic Parts

Part Name

Type

Description

Status

Length (bp)

BBa_K3814001

cds

nahR gene in P. putida + promoter + RBS

New

1015

BBa_K3814002

promoter

Psal1 (PsalTTC, 100% Activity)

New

116

BBa_K3814003

dna

RiboJ ribozyme insulator

New

83

BBa_K3814004

cds

fuGFP (blue + UV excitable variant S65T) + RBS

New

756

BBa_K3814005

promoter

Psal1 (PsalTTC, 34% Activity)

New

116

BBa_K3814006

cds

ComA (ComEC) + RBS, ACIAD2639

New

2401

BBa_K3814007

cds

ComEA (ADP1) + RBS, ACIAD3064

New

402

BBa_K3814008

cds

ComF (ADP1) + RBS, ACIAD3236

New

656

BBa_K3814009

binding

5' Homology Arm for Cluster 2

New

50

BBa_K3814010

cds

ComNOL + RBS, ACIAD3359/ACIAD3357/ACIAD3356

New

1911

BBa_K3814011

cds

PilE + RBS, ACIAD3314

New

447

BBa_K3814012

cds

fimT + RBS, ACIAD695

New

539

BBa_K3814013

binding

5' Homology Arm for Cluster 3

New

50

BBa_K3814014

cds

PilB + RBS, ACIAD362

New

1726

BBa_K3814015

cds

PilU + RBS, ACIAD362

New

1188

BBa_K3814016

cds

PilT + RBS, ACIAD362

New

1085

BBa_K3814017

binding

5' Homology Arm for Cluster 4

New

50

BBa_K3814018

cds

FosC2 gene

New

408

BBa_K3814019

cds

FimU/PilV + RBS, ACIAD3321/ACIAD3319

New

993

BBa_K3814020

cds

ComE + RBS, ACIAD3315

New

522

BBa_K3814021

cds

ComC + RBS, ACIAD361

New

1241

BBa_K3814022

cds

ComM + RBS, ACIAD3360

New

1085

BBa_K3814023

binding

5' Homology Arm for Cluster 5

New

50

BBa_K3814024

promoter

cat promoter (chloramphenicol acetyltransferase)

New

103

BBa_K3814025

cds

CmR gene

New

663

BBa_K3814026

cds

ComB/PilX + RBS, ACIAD3318/ACIAD3317

New

1647

BBa_K3814027

cds

ComQ + RBS, ACIAD3355

New

2139

BBa_K3814028

binding

5' Homology Arm for Cluster 6

New

50

BBa_K3814029

promoter

Pc promoter

New

29

BBa_K3814030

cds

GmR gene

New

537

BBa_K3814031

cds

PilF + RBS, ACIAD558

New

813

BBa_K3814032

cds

PilD + RBS, ACIAD360

New

880

BBa_K3814033

binding

5' Homology Arm for Cluster 7

New

50

BBa_K3814034

cds

ComP + RBS, ACIAD3338

New

455

BBa_K3814035

binding

5' Homology Arm for Cluster 8

New

50

BBa_K3814036

cds

ComC + RBS, ACIAD3316

New

4367

BBa_K3814037

cds

QacE gene

New

336

BBa_K3814038

binding

fliK upstream homology region

New

63

BBa_K3814040

dna

Terminator from ECK120033736.

New

53

BBa_K3814045

promoter

P3BCD2 promoter

New

41

BBa_K3814046

misc

Intermediate DNA

New

77

BBa_K3814047

rbs

RBS for TcR gene

New

9

BBa_K3814048

misc

Intermediate DNA

New

6

BBa_K3814050

binding

fliK downstream homology region

New

50

BBa_K3814051

dna

KpnI site

New

7

BBa_K3814052

dna

HindIII site

New

6

BBa_K3814053

misc

Intermediate DNA

New

14

BBa_K3814055

cds

TpR gene with no RBS

New

246

BBa_K3814056

dna

BamHI site

New

6

BBa_K3814057

cds

AmpR and Promoter 

New

969

BBa_K3814058

misc

Intermediate DNA

New

14

BBa_K3814062

misc

Intermediate DNA

New

11

BBa_K3814063

misc

Intermediate DNA

New

8

BBa_K3814064

misc

Intermediate DNA

New

6

BBa_K3814065

cds

BBa_K523001 (Already in the registry), no RBS

New

2034

BBa_K3814066

misc

Intermediate DNA

New

14

BBa_J31007 

cds

TcR gene

Existing

1191

BBa_J23104 

promoter

Promoter

Existing

35

BBa_B0034 

rbs

RBS

Existing

12

BBa_B0024 

terminator

Terminator

Existing

95

BBa_K329000 

cds

TcR gene

Existing

1210

Composite Parts

Part Name

Type

Description

Status

Length

BBa_K3814067

Composite

fliK Landing Pad (34%)

New

3522

BBa_K3814068

Composite

fliK Landing Pad (100%)

New

3522

BBa_K3814069

Composite

Cluster 1

New

4174

BBa_K3814070

Composite

Cluster 2

New

4274

BBa_K3814071

Composite

Cluster 3

New

4876

BBa_K3814072

Composite

Cluster 4

New

5015

BBa_K3814073

Composite

Cluster 5

New

4948

BBa_K3814074

Composite

Cluster 6

New

3370

BBa_K3814075

Composite

Cluster 7

New

2958

BBa_K3814076

Composite

Cluster 8

New

5128



Composite Part Name

Basic Parts

BBa_K3814067

BBa_K3814038, BBa_K3814040, BBa_K3814001, BBa_K3814005, BBa_K3814003, BBa_K3814004, BBa_K3814045, BBa_K3814046, BBa_K3814047, BBa_K3814048, BBa_J31007, [TAA], BBa_B1006, BBa_K3814050

BBa_K3814068

BBa_K3814038, BBa_K3814040, BBa_K3814001, BBa_K3814002, BBa_K3814003, BBa_K3814004, BBa_K3814045, BBa_K3814046, BBa_K3814047, BBa_K3814048, BBa_J31007, [TAA], BBa_B1006, BBa_K3814050

BBa_K3814069

BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814006, BBa_K3814007, BBa_K3814008, BBa_K3814052, BBa_K3814009, BBa_J23104, BBa_K3814053, BBa_B0034, BBa_K3814055, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814070

BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814010, BBa_K3814011, BBa_K3814012, BBa_K3814052, BBa_K3814013, BBa_K3814057, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814071

BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814014, BBa_K3814015, BBa_K3814016, BBa_K3814052, BBa_K3814017, BBa_J23104, BBa_K3814058, BBa_B0034, BBa_K3814018, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814072

BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814019, BBa_K3814020, BBa_K3814021, BBa_K3814022, BBa_K3814052, BBa_K3814023, BBa_K3814024, BBa_K3814025,BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814073

BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814026, BBa_K3814027,  BBa_K3814052, BBa_K3814028, BBa_K3814029, BBa_K3814030, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814074

BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814031, BBa_K3814032, BBa_K3814052, BBa_K3814033, BBa_J23104, BBa_K3814062, BBa_B0032, BBa_K329000, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814075

BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814034, BBa_K3814052, BBa_K3814035,  BBa_J23104, BBa_K3814063, BBa_B0034, BBa_K3814064, BBa_K3814065, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050

BBa_K3814076

BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814036, BBa_K3814052, BBa_J23104, BBa_K3814066, BBa_B0034, BBa_K3814064, BBa_K3814037, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050