Overview
The following is a table of our basic and composite parts. Our composite parts are our Babushka Blocks, which include the eight gene clusters and the two fliK landing pads.
Basic Parts
Part Name |
Type |
Description |
Status |
Length (bp) |
BBa_K3814001 |
cds |
nahR gene in P. putida + promoter + RBS |
New |
1015 |
BBa_K3814002 |
promoter |
Psal1 (PsalTTC, 100% Activity) |
New |
116 |
BBa_K3814003 |
dna |
RiboJ ribozyme insulator |
New |
83 |
BBa_K3814004 |
cds |
fuGFP (blue + UV excitable variant S65T) + RBS |
New |
756 |
BBa_K3814005 |
promoter |
Psal1 (PsalTTC, 34% Activity) |
New |
116 |
BBa_K3814006 |
cds |
ComA (ComEC) + RBS, ACIAD2639 |
New |
2401 |
BBa_K3814007 |
cds |
ComEA (ADP1) + RBS, ACIAD3064 |
New |
402 |
BBa_K3814008 |
cds |
ComF (ADP1) + RBS, ACIAD3236 |
New |
656 |
BBa_K3814009 |
binding |
5' Homology Arm for Cluster 2 |
New |
50 |
BBa_K3814010 |
cds |
ComNOL + RBS, ACIAD3359/ACIAD3357/ACIAD3356 |
New |
1911 |
BBa_K3814011 |
cds |
PilE + RBS, ACIAD3314 |
New |
447 |
BBa_K3814012 |
cds |
fimT + RBS, ACIAD695 |
New |
539 |
BBa_K3814013 |
binding |
5' Homology Arm for Cluster 3 |
New |
50 |
BBa_K3814014 |
cds |
PilB + RBS, ACIAD362 |
New |
1726 |
BBa_K3814015 |
cds |
PilU + RBS, ACIAD362 |
New |
1188 |
BBa_K3814016 |
cds |
PilT + RBS, ACIAD362 |
New |
1085 |
BBa_K3814017 |
binding |
5' Homology Arm for Cluster 4 |
New |
50 |
BBa_K3814018 |
cds |
FosC2 gene |
New |
408 |
BBa_K3814019 |
cds |
FimU/PilV + RBS, ACIAD3321/ACIAD3319 |
New |
993 |
BBa_K3814020 |
cds |
ComE + RBS, ACIAD3315 |
New |
522 |
BBa_K3814021 |
cds |
ComC + RBS, ACIAD361 |
New |
1241 |
BBa_K3814022 |
cds |
ComM + RBS, ACIAD3360 |
New |
1085 |
BBa_K3814023 |
binding |
5' Homology Arm for Cluster 5 |
New |
50 |
BBa_K3814024 |
promoter |
cat promoter (chloramphenicol acetyltransferase) |
New |
103 |
BBa_K3814025 |
cds |
CmR gene |
New |
663 |
BBa_K3814026 |
cds |
ComB/PilX + RBS, ACIAD3318/ACIAD3317 |
New |
1647 |
BBa_K3814027 |
cds |
ComQ + RBS, ACIAD3355 |
New |
2139 |
BBa_K3814028 |
binding |
5' Homology Arm for Cluster 6 |
New |
50 |
BBa_K3814029 |
promoter |
Pc promoter |
New |
29 |
BBa_K3814030 |
cds |
GmR gene |
New |
537 |
BBa_K3814031 |
cds |
PilF + RBS, ACIAD558 |
New |
813 |
BBa_K3814032 |
cds |
PilD + RBS, ACIAD360 |
New |
880 |
BBa_K3814033 |
binding |
5' Homology Arm for Cluster 7 |
New |
50 |
BBa_K3814034 |
cds |
ComP + RBS, ACIAD3338 |
New |
455 |
BBa_K3814035 |
binding |
5' Homology Arm for Cluster 8 |
New |
50 |
BBa_K3814036 |
cds |
ComC + RBS, ACIAD3316 |
New |
4367 |
BBa_K3814037 |
cds |
QacE gene |
New |
336 |
BBa_K3814038 |
binding |
fliK upstream homology region |
New |
63 |
BBa_K3814040 |
dna |
Terminator from ECK120033736. |
New |
53 |
BBa_K3814045 |
promoter |
P3BCD2 promoter |
New |
41 |
BBa_K3814046 |
misc |
Intermediate DNA |
New |
77 |
BBa_K3814047 |
rbs |
RBS for TcR gene |
New |
9 |
BBa_K3814048 |
misc |
Intermediate DNA |
New |
6 |
BBa_K3814050 |
binding |
fliK downstream homology region |
New |
50 |
BBa_K3814051 |
dna |
KpnI site |
New |
7 |
BBa_K3814052 |
dna |
HindIII site |
New |
6 |
BBa_K3814053 |
misc |
Intermediate DNA |
New |
14 |
BBa_K3814055 |
cds |
TpR gene with no RBS |
New |
246 |
BBa_K3814056 |
dna |
BamHI site |
New |
6 |
BBa_K3814057 |
cds |
AmpR and Promoter |
New |
969 |
BBa_K3814058 |
misc |
Intermediate DNA |
New |
14 |
BBa_K3814062 |
misc |
Intermediate DNA |
New |
11 |
BBa_K3814063 |
misc |
Intermediate DNA |
New |
8 |
BBa_K3814064 |
misc |
Intermediate DNA |
New |
6 |
BBa_K3814065 |
cds |
BBa_K523001 (Already in the registry), no RBS |
New |
2034 |
BBa_K3814066 |
misc |
Intermediate DNA |
New |
14 |
BBa_J31007 |
cds |
TcR gene |
Existing |
1191 |
BBa_J23104 |
promoter |
Promoter |
Existing |
35 |
BBa_B0034 |
rbs |
RBS |
Existing |
12 |
BBa_B0024 |
terminator |
Terminator |
Existing |
95 |
BBa_K329000 |
cds |
TcR gene |
Existing |
1210 |
Composite Parts
Part Name |
Type |
Description |
Status |
Length |
BBa_K3814067 |
Composite |
fliK Landing Pad (34%) |
New |
3522 |
BBa_K3814068 |
Composite |
fliK Landing Pad (100%) |
New |
3522 |
BBa_K3814069 |
Composite |
Cluster 1 |
New |
4174 |
BBa_K3814070 |
Composite |
Cluster 2 |
New |
4274 |
BBa_K3814071 |
Composite |
Cluster 3 |
New |
4876 |
BBa_K3814072 |
Composite |
Cluster 4 |
New |
5015 |
BBa_K3814073 |
Composite |
Cluster 5 |
New |
4948 |
BBa_K3814074 |
Composite |
Cluster 6 |
New |
3370 |
BBa_K3814075 |
Composite |
Cluster 7 |
New |
2958 |
BBa_K3814076 |
Composite |
Cluster 8 |
New |
5128 |
Composite Part Name |
Basic Parts |
BBa_K3814067 |
BBa_K3814038, BBa_K3814040, BBa_K3814001, BBa_K3814005, BBa_K3814003, BBa_K3814004, BBa_K3814045, BBa_K3814046, BBa_K3814047, BBa_K3814048, BBa_J31007, [TAA], BBa_B1006, BBa_K3814050 |
BBa_K3814068 |
BBa_K3814038, BBa_K3814040, BBa_K3814001, BBa_K3814002, BBa_K3814003, BBa_K3814004, BBa_K3814045, BBa_K3814046, BBa_K3814047, BBa_K3814048, BBa_J31007, [TAA], BBa_B1006, BBa_K3814050 |
BBa_K3814069 |
BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814006, BBa_K3814007, BBa_K3814008, BBa_K3814052, BBa_K3814009, BBa_J23104, BBa_K3814053, BBa_B0034, BBa_K3814055, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814070 |
BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814010, BBa_K3814011, BBa_K3814012, BBa_K3814052, BBa_K3814013, BBa_K3814057, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814071 |
BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814014, BBa_K3814015, BBa_K3814016, BBa_K3814052, BBa_K3814017, BBa_J23104, BBa_K3814058, BBa_B0034, BBa_K3814018, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814072 |
BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814019, BBa_K3814020, BBa_K3814021, BBa_K3814022, BBa_K3814052, BBa_K3814023, BBa_K3814024, BBa_K3814025,BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814073 |
BBa_K3814051, BBa_K3814005, BBa_K3814003, BBa_K3814026, BBa_K3814027, BBa_K3814052, BBa_K3814028, BBa_K3814029, BBa_K3814030, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814074 |
BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814031, BBa_K3814032, BBa_K3814052, BBa_K3814033, BBa_J23104, BBa_K3814062, BBa_B0032, BBa_K329000, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814075 |
BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814034, BBa_K3814052, BBa_K3814035, BBa_J23104, BBa_K3814063, BBa_B0034, BBa_K3814064, BBa_K3814065, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |
BBa_K3814076 |
BBa_K3814051, BBa_K3814002, BBa_K3814003, BBa_K3814036, BBa_K3814052, BBa_J23104, BBa_K3814066, BBa_B0034, BBa_K3814064, BBa_K3814037, BBa_K3814056, BBa_B0024, [TAA], BBa_K3814050 |