Team:Stuttgart/Parts
Our team has developed several parts to confer resistance to UV-B radiation in E. coli or HeLa. Our basic parts code for radiation-resistant proteins, while our composite parts combine these proteins with an inducible Arabinose promoter, a protein coding region, BioBricks, His-tag and linker.
Basic parts
These basic parts are coding dsup sequences that are optimized for the respective host organism, so that they would confer radiation resistance to E. coli and HeLa cells.
| Part name | Part number | Part function | Host organism | Size |
|---|---|---|---|---|
| Dsup for E. coli | BBa_K3780029 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | E. coli (in DH5a and BW25113 strain) | 1338 |
| Dsup for HeLa | BBa_K3780023 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | HeLa cells | 1347 |
Composite parts
Three of our composite parts contain a His-tag with a linker. Two (BBa_K3780019 and BBa_K3780017) of these have the His-tag in the N-terminus of the dsup. In the third (BBa_K3780013), the His-tag is in the C-terminus. In the last (BBa_K3780011) composite parts there is no His-tag. The parts for E. coli also have an arabinose promoter and a protein coding region. The part for HeLa also has a Kozak fragment. All composite parts contain BioBricks, which are BioBrick RFC10 compatible.
| Part name | Part number | Part function | Host organism | Size |
|---|---|---|---|---|
| Wildtype dsup for E. coli | BBa_K3780011 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | E. coli (in DH5a and BW25113 strain) | 1582 |
| C-terminal dsup for E. coli | BBa_K3780013 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | E. coli (in DH5a and BW25113 strain) | 1609 |
| N-terminal dsup for E. coli | BBa_K3780017 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | E. coli (in DH5a and BW25113 strain) | 1609 |
| dsup for HeLa | BBa_K3780019 | DNA binding protein from Ramazzottius varieornatus, provides UV-radiation resistance | HeLa cells | 1409 |