Model
Model of ClyR Expression
Experimental bacteria:E. coli Nissle 1917
Experimental plasmid:
Plasmid A(puc57-kan-mini-J23101-OmpA-araB-TT)
Plasmid B(pBAD-Myc-HisA-OmpA-ClyR-6His-TT).
Plasmid A(puc57-kan-mini-J23101-OmpA-araB-TT)
Plasmid B(pBAD-Myc-HisA-OmpA-ClyR-6His-TT).
Procedure
The araboxylan with final concentration 0, 0.3%, 0.6%, 1.0%, 1.5% and 2.0% (w/v) was added into the system to induce ClyR expression.
The araboxylan with final concentration 0, 0.3%, 0.6%, 1.0%, 1.5% and 2.0% (w/v) was added into the system to induce ClyR expression.
Modeling
Table 1. The protein concentration of the culture supernatant of E. coli with
plasmids A and B.
In order to analyze the relationship between the concentration of araboxylan and
the amount of the induced protein ClyR, we have tested various equations bu Matlab. After rigorous
comparison, we choose to adopt the following equation which is normally used in many biological models:
And its analytic expression will be:
It is an exponential function with a constant to improve the fit. Following are the
modeling results with the fitting degree 0.99 which is higher than the fitting degree (0.92) of the
classical exponential function.
Figure 1. Modeling results of y=beax+c
Figure 2. The fitting curve of y=beax+c
model
Conclusion
According to the proposed model of the relationship between the concentration of
araboxylan and the amount of the induced protein ClyR, it indicates that the amount of induced ClyR
gradually increases by the concentration of araboxylan(< 2%, w/v). This model could be used to help
decide
the concentration range of araboxylan in the future which could ensure the amount of ClyR, namely the
core of our product, to take effects in eliminating Gram-positive bacteria in the oral cavity and
treating dental caries when people use our products.