Team:NJU-China/Parts
NJU-China 2021 iGEM Team Parts
we have used or made several parts during the whole process of our project, each of which has its own functions and features. This year's new parts were integrated with the wisdom of our team members Chenyu Tao and Xinwen Yan and they all displayed different results we expected. Here are the details of our parts, you can click the links for more information.
| Part number | Part name | Part type | Abstract | Matching Medal |
|---|---|---|---|---|
| BBa_K3756001 | GATA3-siRNA-1 | RNA | This part expresses siRNA for GATA3, which can specifically degrade GATA3, an important transcription factor associated with type 2 inflammatory response in TH2 cells. We compare it with the following two GATA3-siRNAs to select the best siRNA. | Bronze |
| BBa_K3756002 | GATA3-siRNA-2 | RNA | This part expresses siRNA for GATA3, which can specifically degrade GATA3. We compare it with another two GATA3-siRNAs to select the best siRNA. | Bronze |
| BBa_K3756003 | GATA3-siRNA-3 | RNA | This part expresses siRNA for GATA3, which can specifically degrade GATA3. We compare it with the above two GATA3-siRNAs to select the best siRNA. And this part is the best. | Silver |
| BBa_K3756004 | CKLF1C19-Lamp2b | Coding | It can express the fusion protein CKLF1C19-Lamp2b, where CKLF1C19 is a CCR3-targeting peptide, so it can specifically target to the surface of TH2 cells. Lamp2b is an exosomal membrane protein. Thus, fusion protein CKLF1C19-Lamp2b was able to target TH2 cells by exosomes secreted by cells expressing this protein. | Bronze |
| BBa_K3756005 | TSLP-siRNA-1 | RNA | It can express siRNA for TSLP, which is a key factor in type 2 inflammation. It will be used to verify its theraputic effect on asthma. We compare it with the following two TSLP-siRNAs to select the best siRNA. | Bronze |
| BBa_K3756006 | TSLP-siRNA-2 | RNA | It can express siRNA for TSLP. It will be used to verify its theraputic effect on asthma. We compare it with another TSLP-siRNAs to select the best siRNA. And this part is the best. | Silver |
| BBa_K3756007 | TSLP-siRNA-3 | RNA | It can express siRNA for TSLP. It will be used to verify its theraputic effect on asthma. We compare it with the above two TSLP-siRNAs to select the best siRNA. | Bronze |
| BBa_K3756008 | RGD-Lamp2b | Coding | It can express the fusion protein RGD-Lamp2b, where RGD is an intergrin-targeting peptide, so it can specifically target epithelial cells. Lamp2b is an exosomal membrane protein. Thus, fusion protein CKLF1C19-Lamp2b was able to target epithelial cells by exosomes secreted by cells expressing this protein. | Bronze |
| BBa_K3756009 | CKLF1C19-Lamp2b-GATA3-siRNA-1 | Plasmid | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein CKLF1C19-Lamp2b, where CKLF1C19 is a CCR3-targeting peptide, so it can specifically target to the surface of TH2 cells. At the same time, the DNA circuit also expresses siRNA of GATA3, which can specifically degrade GATA3, an important transcription factor associated with type 2 inflammatory response in TH2 cells. Most importantly, the DNA circuit has attP and attB sites on both sides of the expression frame. In E.coli ZYCY10P3S2T expression system, Intergrase φ31 can loop out the sequence between attP and attB to form minicircle DNA. The plasmid skeleton will be cleaved by I-SCEL enzyme. (Kay MA, et al. A Robust System for Production of Minicircle DNA Vectors. 2010) In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with the following two CKLF1C19-Lamp2b-GATA3-siRNAs to select the best plasmid. | Bronze |
| BBa_K3756010 | CKLF1C19-Lamp2b-GATA3-siRNA-2 | Plasmid | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein CKLF1C19-Lamp2b. At the same time, the DNA circuit also expresses siRNA of GATA3. In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with another two CKLF1C19-Lamp2b-GATA3-siRNAs to select the best plasmid. | Bronze |
| BBa_K3756011 | CKLF1C19-Lamp2b-GATA3-siRNA-3 | Plasmid | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein CKLF1C19-Lamp2b. At the same time, the DNA circuit also expresses siRNA of GATA3. In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with the above two CKLF1C19-Lamp2b-GATA3-siRNAs to select the best plasmid. And this is the best part. | Silver |
| BBa_K3756012 | RGD-Lamp2b-TSLP-siRNA-1 | Coding | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein RGD-Lam2p, in which RGD is an epithelial-targeting peptide, and therefore can specifically target epithelial integrin. At the same time, the DNA circuit also expresses the siRNA of TSLP, which specifically degrades TSLP, an important factor involved in type 2 inflammation. Most importantly, the DNA circuit has attP and attB sites on both sides of the expression frame. In E. coli ZYCY10P3S2T expression system, Intergrase Φ31 can loop out the sequence between attP and attB to form minicircle DNA. The plasmid skeleton will be cleaved by I-SCEL enzyme. (Kay MA, et al. A Robust System for Production of Minicircle DNA Vectors. 2010) In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with the following two RGD-Lamp2b-TSLP-siRNAs to select the best plasmid. | Bronze |
| BBa_K3756013 | RGD-Lamp2b-TSLP-siRNA-2 | Coding | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein RGD-Lam2p. At the same time, the DNA circuit also expresses the siRNA of TSLP. In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with another two RGD-Lamp2b-TSLP-siRNAs to select the best plasmid. And this is the best part. | Silver |
| BBa_K3756014 | RGD-Lamp2b-TSLP-siRNA-3 | Coding | This DNA circuit is designed to express specific proteins and siRNA that specifically target TH2 cells and down-regulate the expression level of relevant target mRNA. It can express the fusion protein RGD-Lam2p. At the same time, the DNA circuit also expresses the siRNA of TSLP. In our project, the DNA circuit will be prepared in E.coli ZYCY10P3S2T expression system to form Minicircle DNA. We compare it with the above two RGD-Lamp2b-TSLP-siRNAs to select the best plasmid. | Bronze |
| BBa_K3756015 | GFP-siRNA | RNA | It can express siRNA for GFP，which will downregulate GFP mRNA expression and thus lead to fluorescence quenching. We plan to use it in mice to verify the tissue specificity of our therapy. | Bronze |
| BBa_K3756016 | RGD-Lamp2b-GFP-siRNA | Coding | This will lead to fluorescence quenching in lung epithelial cells,which will be used to verify the tissue specificity of our therapy. | Bronze |