Team:MADRID UCM/Contribution
Contribution
After almost a year working on the design and development of the 4C_Fuels project, our iGEM team has generated valuable tools and knowledge for the global synthetic biology community. In this page you will read about our contributions to the community.
Regulatory parts for cyanobacteria
Within the wet lab part of our project we have successfully assembled a collection of phytobrick compatible parts, following the standard part design of the Marburg Collection.
Among these parts there multiple regulatory elements for cyanobacteria, remarking the super-strong constitutive promoters PpsbA-RiboJ BBa_K3726079 , Prbcl-m36 (BBa_K3726080) and PS-PR ( BBa_K3726084). All of them are promoters which have demonstrated very high expression levels in Synechococcus elongatus genre as well as related cyanobacteria species.
Besides these parts, other regulatory elements like in-silico designed strong RBS, regulated promoters (Pgntk and a AraC-pBAD device) and one cyanobacterial specific strong terminator have also been assembled as Phytobrick compatible parts. We have identified and assembled a set of 6 parts corresponding with homology regions for double homologous recombination in Synechococcus elongatus PCC11801.
With the creation of these and more elements that you can explore in our Part Collection page we aim to expand the availability of genetic elements for cyanobacteria.
Identification of Novel Neutral Sites
NSFinder is a python script designed by our team for the identification of potential integration sites within any prokaryotic genome. Our aim with the development of this software piece was to provide the community with a tool for fast identification of potential neutral sites within almost any unconventional prokaryotic chassis. This code just requires the genome sequence information of an organism to rapidly provide a list of potential genomic loci for integration without detrimental effects in cellular fitness
To get the code, knowing more about how does it works and discover how to easily design homology regions for double homologous recombination. Visit our software page.
Besides these parts, other regulatory elements like in-silico designed strong RBS, regulated promoters (Pgntk and a AraC-pBAD device) and one cyanobacterial specific strong terminator have also been assembled as Phytobrick compatible parts. We have identified and assembled a set of 6 parts corresponding with homology regions for double homologous recombination in Synechococcus elongatus PCC11801.
Raising a phototrophic chassis community
In this iGEM season we have seen how many teams have decided to work with phototrophic organisms within their projects. We truly believe in the incredible potential of photosynthetic organisms. Thus we decided to network and collaborate with other teams, in order to create a common body of knowledge and sharing ideas. This is how we joined Marburg and Bielefeld-CeBiTec teams in the adventure of building the iGEM phototrophs community.
The final result of this huge collaborative work has been a phototroph’s manual for future iGEM teams. To know more about our role and how this community has contributed to iGEM you can visit our Phototrophs Synthetic biology page.