Team:FAFU-CHINA/Notebook

Notebook

DESCRIPTION

Memorabilia of the experimental group
DATE	EVENT
2021.2.27	Screening test, written test
2021.3.6	The interview part, the first design of relevant synthetic biology topics, lasted for one month. 2021.4.6 The first project report and personnel selection, exchange of literature review methods, theoretical research of synthetic biology.
2021.4.9	The establishment of the experimental group and the design of the second synthetic biology project can improve the previous project or choose a new project. Requirements, meet the basic principles of synthetic biology, and meet the basic design conditions such as biosafety, experimental design and economic saving. Finally, the best topic of iGEM this year will be selected under the review of teachers.
2021.4.15	At the first group meeting, members told other group members about the progress and achievements of their research, listened to their opinions and improved them.
2021.4.27	In the second group meeting, prepare the PPT, communicate and improve the topic.
2021.5.6	The teacher reviewed the subject and appointed Chen Qinyu as the leader of the experimental group, and the microbial aroma production plant as the iGEM subject in 2021. All members of the experimental group will assist in the follow-up theoretical design and specific experimental design .
2021.5.12	In the third group meeting, for the follow-up experimental design, the team members need to find suitable chemical elements and culture medium.
2021.5.18	The fourth group meeting will exchange the reading results of the paper for a week. At the same time, the experimental group will discuss the specific experimental design from next week. The team members need to put forward effective experimental specific schemes according to the basic experiments required in the early stage.
2021.6.23	The fifth group meeting, the time arrangement of summer school retention personnel, the arrangement of summer experiment, the expansion and extension of the subject, and the discussion of the final experimental method.
2021.7.1	The sixth group meeting, change of school stay time in summer vacation, design of publicity video content and temporary experimental adjustment.
2021. 8.28	The school starts, the team members return to school, and the instructor arranges laboratory training.
2021.9.4	Escherichia coli transformation experiment. Transform our B3 plasmid into the DH5α strain, and inoculate the transformed DH5α into LB plates containing chloramphenicol.
2021.9.5	Pick the Escherichia coli transformants in the resistant plate, and perform bacterial liquid PCR. Use a fluorescent stereo microscope to observe the petri dish, and combine the PCR results to select the correct transformants.
2021.9.13	The first growth curve determination is over, the growth of E. coli and yeast is good, and the co-cultivation medium is successfully designed
2021.9.15	Preparation of Saccharomyces cerevisiae (PS100) protoplasts
2021.9.23	Pick several yeast transformants from the resistant plates, inoculate them into YPD medium with related antibiotics resistance, and extract the total DNA.
2021.9.27	Pick several yeast transformants from the resistant plates and inoculate them into YPD medium with related antibiotics resistance to prepare total DNA extraction.
2021.10.1	Use the total DNA template to perform PCR to select the correct yeast transformants.
2021.10.02	Yeast liquid PCR:5ul bacterial solution was added into 20ul 0.02m NaOH solution and lysed at 99℃ for 10 minutes. The supernatant was used as template for PCR identification
2021.10.02-2021.10.09	We conducted a seven-day co-cultivation experiment and drew the corresponding growth curve. We set the E. coli culture as group A, yeast culture as group B, and the mixed culture of the two as group C, and each group had 3 bottles of bacterial liquid at the same time. nourish. The cultivation was started at 4 o'clock in the afternoon of the same day, and the corresponding amount of linalool and nerol were added after the growth of No. 3 in group A, No. 3 in group B, and No. 3 in group C reached the growth burst. After No. 1 of group A, No. 1 of group B, and No. 1 of group C reached a stable growth, the corresponding amounts of linalool and nerol were added. Measure the OD value of each bottle of bacterial liquid in real time during the whole experiment.
2021.10.15	Yeast inverters were selected and added into the corresponding resistant medium, and cultured at 30℃ and 180rpm for 3 days. 6ml of bacterial solution was added into aMicro-Reaction Vial and detected by gas chromatography.