Team:FAFU-CHINA/Awards
Bronze
1、Competition Deliverables
We have submitted all deliverables required in the review form.
2、Attributions
https://2021.igem.org/Team:FAFU-CHINA/Attributions
We specify the contributions of team members and non-team members to our team on the assigned page. At the same time, we are on the page, we introduce the development situation and our team in the popularity of synthetic biology in our school.
3、Project Description
https://2021.igem.org/Team:FAFU-CHINA/Description
In the assigned page, we introduced the inspiration for our project, introduced the technical route of our project, and the direction of our project in the future.
4、Contribution
https://2021.igem.org/Team:FAFU-CHINA/Contribution
We verified the basic function of BBa_K3127998, and we also found ways to extend the function of BBa_K3127998 in the literature, hoping to help future teams to build more stable photogenetic systems. We also summarized a year experiences and lessons from the development team, and in accordance with the category proposed many have reference value, hope for the future team.
Silver
1、Engineering Success
https://2021.igem.org/Team:FAFU-CHINA/Engineering
We successfully solved some of the problems in the project, including the stability of the co-culture system and the photogenetic system in E.coli. Due to limited time, we could not fully verify the components of the photogenetic system used in yeast (BBa_K3732000~ BBa_K3732003), but we theoretically verified the feasibility of Parts to predict their functions by using the model. Combined with the literature knowledge and the experimental results, we already know the way of our project. We have also noticed the problem of our project. Aromatic substances are easily transformed into each other, and further optimization of metabolic pathways is needed to achieve the enrichment of single aromatic substances.
2、Collaboration
https://2021.igem.org/Team:FAFU-CHINA/Collaborations
We have conducted experiments, HP, modeling and reached cooperation with FZU-China, Tongji_China, SZPT-China, SZ-SHD, NWU-China-A, ZJU-China, HZAU-China, CAU-China and other schools.
3、Human Practices
https://2021.igem.org/Team:FAFU-CHINA/Human_Practices
Before carrying out HP activities, we consulted experts for relevant information and studied relevant laws and regulations to ensure the correct implementation of human practices. We consulted a lot of relevant literature and interviewed many experts to constantly improve our project. We also conducted surveys on campus and in the community, asking the public about their smell preferences and approval of the program.
4、Proposed Implementation
https://2021.igem.org/Team:FAFU-CHINA/Implementation
Our project designs a photoinduced microbial culture system. We also designed a microbiome night light to bring our project to life. With the change of light intensity, people can smell the dynamic change of aroma throughout the day. Strains of linalool is contained in the production of fragrance, it can help alleviate the negative emotions of people. We hope to bring different experience to those who love aromatherapy.
Gold
1、Integrated Human Practices
https://2021.igem.org/Team:FAFU-CHINA/Human_Practices
From learning how to properly carry out human practice work, to collecting opinions from experts and the public and improving the project, to the entrepreneurship and promotion of the project, human practice work is implemented in our project. Through a large number of social investigations, we have fully understood the market background of spices, and also adopted the opinions of many people and experts to fully consider the safety and practicality of the project. We also visited factories and communities, and with their input, we improved our design to meet their needs. Based on the results of human practice, we also designed a microbial-scented night light, and learned about the follow-up development of the project from relevant government departments. All in all, our HP work is relatively complete and can be used as a reference for future iGEM teams.
2、Project Modeling
https://2021.igem.org/Team:FAFU-CHINA/Model
First, we decompose the MVA pathway into a single elementary reaction according to the kinetic-based biochemical reaction model and construct a series of constant-coefficient differential equations to predict the relationship between the production of each aroma substance in a single cell and time. Secondly, we established an oscillator model to study the steady-state process of the microbial cell separation cycle. Use the response surface model based on BP neural network to explore and optimize the conditions of the co-cultivation system for the stable existence of E.coli and Saccharomyces cerevisiae, and help the Tongji_China team to support this model. Based on the conformational changes of Jα and helix in LOV2 under light and dark conditions, we use the signal node network model, use the BE-ABC optimization algorithm, and the Alphafold2 deep learning algorithm to predict the structure of the protein, and a series of related bioinformatics analysis. Finally, we also performed physical and mathematical simulations of the aroma emitted by the night light.
3、Proof of Concept
https://2021.igem.org/Team:FAFU-CHINA/Proof_Of_Concept
Our products do not require aseptic operations to mix the bacteria with the culture medium to start the fermentation system. On the other hand, our equipment is very simple, only need night light and fermentation tank to achieve all the desired functions. Our philosophy is to reduce complexity to simplicity, not to make buyers think they are buying large-scale laboratory equipment. This design makes the transportation and use of the product very convenient. Due to the cover made of the filter membrane, the microorganisms in the fermentor will not leak from the fermentor to the environment, and the external microorganisms will not enter the fermentor, thus avoiding the use of antibiotics and strains with resistance genes. Nowadays, aromatherapy has entered the lives of the general public, hoping to bring a new and different experience to those who love aromatherapy.
4、partnership
https://2021.igem.org/Team:FAFU-CHINA/Partnership
Due to the epidemic, our cooperation with other schools is mainly online. After the exchange meeting in China, we have reached a long-term cooperative relationship with FZU-China and Tongji_China. We have maintained close cooperation since the beginning of the project, and have had close communication with FZU-China, Tongji_China and so on.
5、Education and Communication
https://2021.igem.org/Team:FAFU-CHINA/Communication
We carry out educational activities in kindergartens, elementary schools, middle schools, universities and other platforms. We adopt different teaching models for different groups of people, or directly or indirectly introduce synthetic biology and its ideas. We also cooperated with companies to hold exhibitions related to our projects, and to popularize the knowledge of smell to the public, encouraging them to learn about plants and the biological knowledge behind them. In addition, we also publish our works on many online platforms, such as tweets, videos, etc., hoping to let more people know about synthetic biology and our projects. Every educational activity is carefully planned. We will carefully prepare the PPT and manuscripts needed for the speech, and make posters and brochures. Materials related to educational activities, as well as electronic backups, can be found by the masses on the corresponding network platform and further study. Fragrance is a well-known and talked-about topic. We use this as an entry point to encourage the people to use synthetic biology to create their own fragrance.