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                                                             Duke Kunshan University DKU iGEM Team 2021

Safety form:

Our team uses E.coli (BL21 and DH5), as a chassis organism, that does not present minimal potential hazard to laboratory personnel and the environment. As our lab aims to modify E.coli to construct biological systems that can produce algicidal peptide that could selectively degrade Microcystis Aeruginosa, the risks of using algicidal peptide HPA3NT3-A2 and Microcystis Aeruginosa PCC7806 are also under consideration. Biological wastes are managed in accordance with the biohazardous waste disposal policy and procedures as following:
    1. Although previous literature has suggested that HPA3NT3-A2 originated from Helicobacter pylori presents low hemolytic and cytotoxic effects on mammalian cells (Lee & Park, 2020). We considered the possibility that HPA3NT3-A3 might harm other microorganisms, and therefore potentially influence the ecosystem of the water body when escaped from the lab. The high temperature of autoclave (121℃) will lead to inactivation of the peptide.
    2. Microcystis Aeruginosa PCC7806 can potentially cause harmful algal bloom (HAB) when it’s accidently escaped into surrounding bodies of water. Therefore, the liquid waste of algae must be completely decontaminated either chemically (by using disinfectant) and physically (by using autoclave) prior to disposal as specified in the biosafety manual.

Safe Lab Work:

To manage any of the risks that can arise during the lab works, Dr. Suhua Li, a lab manager of DKU biology lab, is overseeing the overall project and providing some training and safety information to ensure that we are following the safety regulations for handling the equipment and disposing of any samples. We received lab safety training every semester, and are well aware of the biosafety law of the People’s Republic of China.
Professor Linfeng Huang, our PI, is overseeing our work and the protocols. Professor Huang, an Associate Professor of Biology at DKU, has at least 20 years of lab experience with microorganisms since he received his bachelor’s degree in 2003. He has reviewed our lab protocols and provided adequate instruction for using lab equipment.

Lee, J. K., & Park, Y. (2020). All d-Lysine Analogues of the Antimicrobial Peptide HPA3NT3-A2 Increased Serum Stability and without Drug Resistance. Int J Mol Sci, 21(16).