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| | {{City_of_London_UK}} | | {{City_of_London_UK}} |
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| | + | <meta http-equiv="X-UA-Compatible" content="IE=edge"> |
| | + | <meta name="viewport" content="width=device-width, initial-scale=1.0"> |
| | + | <title>Parts</title> |
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| | + | <script src="https://polyfill.io/v3/polyfill.min.js?features=es6"></script> |
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| − | <div class="column full_size">
| + | </head> |
| − | <h1>Parts</h1>
| + | |
| − | <p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code><groupparts></code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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| − | <p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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| − | <div class="highlight decoration_background"> | + | <div class=" intro-content HP-cont"> |
| − | <h3>Note</h3>
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| − | <p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
| + | <h1 class="main-header">Parts</h1> |
| − | This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
| + | <p> Here is a list of our parts catalogue. All of our parts are designed to assist with the detection of |
| − | </div>
| + | miR-517 and |
| − | </div>
| + | miR-210, natrually occuring RNAs which are upregulated in preeclampsia patients. Our first four parts |
| − | | + | (BBa_K3905000-BBa_K3905003) made up our first generation toehold switch riboregulator series. These |
| − | <div class="clear extra_space"></div> | + | parts included a |
| − | <div class="line_divider"></div> | + | toehold switch and antiRNA for each of our target miRNAs. Based on in Silico testing, and advice from |
| − | <div class="clear extra_space"></div>
| + | researchers, our |
| − | | + | next two basic parts (BBa_K3905004 and BBa_K3905005) made up the more efficient second series switches. |
| − | | + | These were |
| − | | + | followed by our novel AND-gate toehold switch (BBa_K3905006) which could detect both miRNAs in one |
| − | | + | switch with the help |
| − | | + | of an antiRNA (BBa_K3905007). We then made composites of our toehold switches with the reporter protein |
| − | <div class="column full_size">
| + | luciferin |
| − | <div class="highlight decoration_B_full">
| + | (BBa_K3905008-BBa_K3905012). Finally, our last eight basic parts make up our isothermal amplification |
| − | | + | system. Using |
| − | <h3>Adding parts to the Registry</h3>
| + | these DNA probes we are able to increase the concentration of our target miRNAs.</p> |
| − | <p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p> | + | <iframe src="https://static.igem.org/mediawiki/2021/6/6e/T--City_of_London_UK--Parts.pdf" width="100%" |
| − | | + | style="max-width: 1000px; height: 1000px;"> |
| − | <p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
| + | </iframe> |
| − | <div class="button_link">
| + | </div> |
| − | <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
| + | </div> |
| − | ADD PARTS
| + | </body> |
| − | </a>
| + | |
| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="column third_size">
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| − | <div class="highlight decoration_A_full">
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| − | <h3>Inspiration: Basic Parts</h3>
| + | |
| − | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
| + | |
| − | | + | |
| − | <p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
| + | |
| − | </ul>
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| − | </div>
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| − | </div>
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| − | <div class="column third_size">
| + | |
| − | <div class="highlight decoration_A_full">
| + | |
| − | <h3>Inspiration: Composite Parts</h3>
| + | |
| − | <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
| + | |
| − | | + | |
| − | <p> You can also take a look at the following examples for Composite Parts on the Registry:</p> | + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
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| − | <li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li> | + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
| + | |
| − | </ul>
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| − | </div> | + | |
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| − | <h3>What information do I need to start putting my parts on the Registry?</h3>
| + | |
| − | <p>The information needed to initially create a part on the Registry is:</p>
| + | |
| − | <ul>
| + | |
| − | <li>Part Name</li>
| + | |
| − | <li>Part type</li>
| + | |
| − | <li>Creator</li>
| + | |
| − | <li>Sequence</li>
| + | |
| − | <li>Short Description (60 characters on what the DNA does)</li>
| + | |
| − | <li>Long Description (Longer description of what the DNA does)</li>
| + | |
| − | <li>Design considerations</li>
| + | |
| − | </ul>
| + | |
| − | | + | |
| − | <p>
| + | |
| − | We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
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| − | <h3>Part Table </h3>
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| − | | + | |
| − | <p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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| − | </html>
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| − | <groupparts>iGEM20 City_of_London_UK</groupparts>
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Parts
Here is a list of our parts catalogue. All of our parts are designed to assist with the detection of
miR-517 and
miR-210, natrually occuring RNAs which are upregulated in preeclampsia patients. Our first four parts
(BBa_K3905000-BBa_K3905003) made up our first generation toehold switch riboregulator series. These
parts included a
toehold switch and antiRNA for each of our target miRNAs. Based on in Silico testing, and advice from
researchers, our
next two basic parts (BBa_K3905004 and BBa_K3905005) made up the more efficient second series switches.
These were
followed by our novel AND-gate toehold switch (BBa_K3905006) which could detect both miRNAs in one
switch with the help
of an antiRNA (BBa_K3905007). We then made composites of our toehold switches with the reporter protein
luciferin
(BBa_K3905008-BBa_K3905012). Finally, our last eight basic parts make up our isothermal amplification
system. Using
these DNA probes we are able to increase the concentration of our target miRNAs.
