Difference between revisions of "Team:Fujian United/Notebook"

(Replaced content with "https://2021.igem.org/Team:Fujian_United")
 
Line 1: Line 1:
−
https://2021.igem.org/Team:Fujian_United
+
<html>
 +
 
 +
<link rel="stylesheet" type="text/css"
 +
      href="https://2021.igem.org/wiki/index.php?title=Template:Fujian_United/StyleCSS&action=raw&ctype=text/css"/>
 +
 
 +
<div class="alpha-luco-content notebook-page">
 +
    <!--navbar-->
 +
    <div class="navbar">
 +
        <a class="logo" href="https://2021.igem.org/Team:Fujian_United">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/f3/T--Fujian_United--logo.jpeg" alt="">
 +
        </a>
 +
 
 +
        <!--nav-->
 +
        <ul class="list">
 +
            <!--Team-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Team">Team</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Team">Team Members</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Attributions">Attributions</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Collaborations">Collaboration</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
 +
 
 +
            <!--Modeling-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Model">Modeling</a>
 +
            </li>
 +
 
 +
            <!--Entrepreneurship-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Entrepreneurship">Entrepreneurship</a>
 +
            </li>
 +
 
 +
            <!--Implementation-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Implementation">Implementation</a>
 +
            </li>
 +
 
 +
            <!--Human Practice-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Human Practice</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Integrated Human
 +
                                Practice</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Communication">Communication</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Fundraising">Fundraising</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
 +
 
 +
            <!--Parts-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Parts">Parts</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Parts">Parts
 +
                                Collection</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Engineering">Engineering</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
 +
 
 +
            <!--Project-->
 +
            <li class="item active">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Description">Project</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Description">Description</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Experiments">Experiments</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Results">Results</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Proof_Of_Concept">Proof of Concept</a>
 +
                        </li>
 +
                        <li class="child-item active">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Notebook">Notebook</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Safety">Safety</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
 +
 
 +
            <!--Home-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United">Home</a>
 +
            </li>
 +
        </ul>
 +
    </div>
 +
 
 +
    <!--图片-->
 +
    <div class="page-header">
 +
        <img src="https://static.igem.org/mediawiki/2021/9/94/T--Fujian_United--bg_1.png" alt="">
 +
    </div>
 +
 
 +
    <!--内容-->
 +
    <div class="content-middle-outer">
 +
        <div class="content-middle">
 +
            <section class="article p-t-30 p-b-54">
 +
                <h1 class="content-header">Notebook</h1>
 +
 
 +
                <img class="m-b-24" src="https://static.igem.org/mediawiki/2021/c/c8/T--Fujian_United--img_notebook_1.jpg" alt="" style="width: 90%;">
 +
 
 +
                <table>
 +
                    <tr>
 +
                        <td colspan="2" class="notebook-head">July</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td style="width: 20%">4<sup>th</sup></td>
 +
                        <td>Prepare culture mediums.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>5<sup>th</sup></td>
 +
                        <td>
 +
                            Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.
 +
                            <img class="m-b-24" src="https://static.igem.org/mediawiki/2021/d/d6/T--Fujian_United--img_notebook_2.png" alt=""
 +
                                style="width: 90%;">
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>6<sup>th</sup></td>
 +
                        <td>Construction of the 4 plasmids.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>7<sup>th</sup></td>
 +
                        <td>
 +
                            Colony PCR, gel electrophoresis, and select single clones.
 +
                            <img class="m-b-24" src="https://static.igem.org/mediawiki/2021/5/57/T--Fujian_United--img_notebook_3.png" alt=""
 +
                                style="width: 80%; margin: 0 auto; display: block;">
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>8<sup>th</sup></td>
 +
                        <td>Plasmids extraction from E. Coli, and sent to the DNA sequencing company.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>9<sup>th</sup></td>
 +
                        <td>Verify the DNA sequencing resuls and carry out the transformation of plasmids into the
 +
                            Saccharomyces cerevisiae by Heat-Shock approach.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>11<sup>st</sup></td>
 +
                        <td>Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in
 +
                            1ml culture mediums.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>12<sup>nd</sup></td>
 +
                        <td>Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate
 +
                            efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>13<sup>th</sup></td>
 +
                        <td>Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2" class="notebook-head">August</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2">
 +
                            Repeat the plasmids construction experiments and the yeast culture by AQ yeast.
 +
                            <img class="m-b-24" src="https://static.igem.org/mediawiki/2021/e/e0/T--Fujian_United--img_notebook_4.jpg" alt=""
 +
                                style="width: 60%; margin: 0 auto; display: block;">
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2" class="notebook-head">September</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2">Carry out enzyme activity tests and fermentation performance tests.</td>
 +
                    </tr>
 +
                </table>
 +
            </section>
 +
        </div>
 +
    </div>
 +
 
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content-middle">
 +
            <hr>
 +
            <span>WeChat Official Account:</span>
 +
            <span class="red">iGEM Alpha Luco</span>
 +
        </div>
 +
    </div>
 +
</div>
 +
</html>

Latest revision as of 12:17, 20 October 2021

Notebook

July
4th Prepare culture mediums.
5th Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.
6th Construction of the 4 plasmids.
7th Colony PCR, gel electrophoresis, and select single clones.
8th Plasmids extraction from E. Coli, and sent to the DNA sequencing company.
9th Verify the DNA sequencing resuls and carry out the transformation of plasmids into the Saccharomyces cerevisiae by Heat-Shock approach.
11st Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in 1ml culture mediums.
12nd Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
13th Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
August
Repeat the plasmids construction experiments and the yeast culture by AQ yeast.
September
Carry out enzyme activity tests and fermentation performance tests.
WeChat Official Account: iGEM Alpha Luco