Safety
Attention should be paid at all stages of sample collection, extraction and amplification:
Wear disposable gloves. If the reaction liquid is splashed accidentally, change the gloves immediately.
Use disposable suction head, which is strictly prohibited to be mixed with the suction head of PCR product analysis room. The suction head should not be exposed to the air for a long time to avoid aerosol pollution.
To avoid the spatter of the reaction liquid, when opening the reaction tube, collect the liquid in the bottom of the tube by centrifugal slightly before opening the cover. If accidentally splashed on the gloves or the table, should immediately change the gloves and wipe the table with dilute acid.
When using electrical equipment (such as oven, constant temperature water bath, centrifuge, electric stove, etc.), strictly prevent electric shock.
When using the water bath to heat the liquid, it is necessary to check regularly to avoid overflow.
Gloves should be worn when handling acid and base solutions, and should be handled as soon as possible to avoid the reaction of the solution with air.
The experiments which can produce irritating or toxic gases must be carried out in the ventilator.
With a strong carcinogenic and corrosive solution, use special care, do not make it splash on the clothes or skin, and avoid direct contact. Waste acid should be poured into the acid tank, but do not pour lye into the acid tank, so as not to neutralize the acid and base and release a lot of heat and risk.
Be careful when heating the lights. When the alcohol lamp is burning out and the lamp is not extinguished, do not inject fuel; When the alcohol lamp is out, use the lamp cap to cover, do not blow with the mouth to prevent accidents; The lit matches should be extinguished immediately after being used up and should not be thrown about.
When using UV light instruments, we should pay attention to wearing opaque gloves to reduce the damage to human cells and skin.
Project Safety Information
In the lab, all experiments were conducted under the supervision of our instructor, Dr. Li and we all received intensive training before we started.
The strains we used in this project include the Saccharomyces cerevisiae (CEN.PK2-1C; AQ yeast) and Escherichia coli (DH5α), which are all commercially and commonly used with little harm to human beings.