| July |
| 4th |
Prepare culture mediums. |
| 5th |
Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.
|
| 6th |
Construction of the 4 plasmids. |
| 7th |
Colony PCR, gel electrophoresis, and select single clones.
|
| 8th |
Plasmids extraction from E. Coli, and sent to the DNA sequencing company. |
| 9th |
Verify the DNA sequencing resuls and carry out the transformation of plasmids into the
Saccharomyces cerevisiae by Heat-Shock approach.
|
| 11st |
Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in
1ml culture mediums.
|
| 12nd |
Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate
efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
|
| 13th |
Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
|
| August |
Repeat the plasmids construction experiments and the yeast culture by AQ yeast.
|
| September |
| Carry out enzyme activity tests and fermentation performance tests. |
