Difference between revisions of "Team:Shanghai City United/Contribution"

(Prototype team page)
 
 
Line 1: Line 1:
−
{{IGEM_TopBar}}
+
<html lang="en">
−
{{Shanghai_City_United}}
+
−
<html>
+
  
−
<div class="column full_size judges-will-not-evaluate">
+
<head>
−
<h3>★  ALERT! </h3>
+
    <meta charset="UTF-8">
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2021.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2021.igem.org/Judging/Awards"> award listed below</a>. </p>
+
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2021.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
−
</div>
+
    <title>Shanghai_City_United</title>
 +
    <link rel="stylesheet"
 +
        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_City_United/Main_CSS&action=raw&ctype=text/css" />
 +
</head>
  
 +
<body>
 +
    <nav class="head-nav clearfix">
 +
        <div class="top-block"></div>
 +
        <div class="top-nav-bar">
 +
            <ul class="clearfix">
 +
                <span class="small-logo"></span>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_City_United">Home</a>
 +
                </li>
 +
                <li>
 +
                    <a href="">Project</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Description"
 +
                                    class="sub-nav-74">Description</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Experiments"
 +
                                    class="sub-nav-74">Experiments</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Results"
 +
                                    class="sub-nav-74">Results</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Proof_Of_Concept"
 +
                                    class="sub-nav-52">Proof Of
 +
                                    Concept</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Notebook"
 +
                                    class="sub-nav-52">Notebook</a>
 +
                            </li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Safety">Safety</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li class="active">
 +
                    <a href="">Parts</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Parts" class="sub-nav-74">Parts
 +
                                    Collection</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Engineering"
 +
                                    class="sub-nav-74">Engineering</a></li>
 +
                            <li class="current-sub-nav"><a href="#" class="sub-nav-74">Contribution</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <a href="">Human Practices</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Human_Practices"
 +
                                    class="sub-nav-74">Integrated
 +
                                    Human Practice</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Communication"
 +
                                    class="sub-nav-74">Communication</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Fundraising"
 +
                                    class="sub-nav-74">Fundraising</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_City_United/Implementation">Implementation</a>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_City_United/Entrepreneurship">Entrepreneurship</a>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_City_United/Model">Model</a>
 +
                </li>
 +
                <li>
 +
                    <a href="">Team</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Members"
 +
                                    class="sub-nav-74">Team
 +
                                    Members</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Attributions"
 +
                                    class="sub-nav-74">Attributions</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Collaborations"
 +
                                    class="sub-nav-74">Collaborations</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_City_United/Partnership"
 +
                                    class="sub-nav-74">Partnership</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
            </ul>
 +
        </div>
 +
    </nav>
 +
    <div class="sub-banner">
 +
        <img src="https://static.igem.org/mediawiki/2021/2/23/T--Shanghai_City_United--Description01.png" alt="" />
 +
    </div>
 +
    <div class="sub-content no-margin-top">
 +
        <div class="sub-title">Contribution</div>
 +
        <div class="article-title">BBa_K4093004 </div>
 +
        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
 +
            Name: pMD19-T-xynA<br />
 +
            Base Pairs: 3346bp<br />
 +
            Origin: Bacillus subtilis ,synthetic<br />
 +
            Properties: Additive enzyme for baby poultry feed
 +
        </div>
 +
        <div class="article-content"><b>== Usage and Biology ==</b></div>
 +
        <div class="article-content">Feed grains are whole grains such as corn, wheat and barley used in the feeding of
 +
            livestock and poultry. At present, corn is the main fodder in the feed industry. Due to the increasing
 +
            shortage of raw materials and the rising price, the development of the feed industry has been greatly
 +
            limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain,
 +
            and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat,
 +
            cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP),
 +
            which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients
 +
            in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore,
 +
            we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed
 +
            absorption efficiency.</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/a/a3/T--Shanghai_City_United--contribution01.jpg" alt="" />
 +
            <span>Figure1. Demonstration of Partnership</span>
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The core product will be a probiotic that can produce xylanase. In this project, we
 +
            designed to construct a expressing xylanase gene plasimd.</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/f7/T--Shanghai_City_United--contribution02.jpg" alt="" />
 +
            <span>Figure 2. Schematic map of expression module of xynA .</span>
 +
        </div>
 +
        <div class="article-title">BBa_K4093005</div>
 +
        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
 +
            Name: PUS-xynA<br />
 +
            Base Pairs: 936bp<br />
 +
            Origin: lactic acid bacteria, Bacillus subtilis ,synthetic<br />
 +
            Properties: Additive enzyme for baby poultry feed
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The core product will be a probiotic that can produce xylanase. In this project, we
 +
            designed to construct a expressing xylanase gene module.</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/fb/T--Shanghai_City_United--contribution03.jpg" alt="" />
 +
            <span>Figure 2. Schematic map of expression module of xynA and PUS.</span>
 +
        </div>
 +
        <div class="article-title">BBa_K4093006</div>
 +
        <div class="article-content"><b>== Profile ==</b></div>
 +
        <div class="article-content">
 +
            Name: pSIP403-PUS-xyn A<br />
 +
            Base Pairs: 8354bp<br />
 +
            Origin: E. coli , Bacillus subtilis ,synthetic<br />
 +
            Properties: Additive enzyme for baby poultry feed
 +
        </div>
 +
        <div class="article-content"><b>== Construct design ==</b></div>
 +
        <div class="article-content">The core product will be a probiotic that can produce xylanase. In this project, we
 +
            designed to construct a plasmid expressing xylanase gene, then we transformed them into Escherichia coli and
 +
            Lactobacillus reuteri for further performance analysis.</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/e/e5/T--Shanghai_City_United--contribution04.jpg" alt="" />
 +
            <span>Figure 2. Schematic map of expression system of xynA plasmids.</span>
 +
        </div>
 +
    </div>
 +
    <footer class="footer">
 +
        <section class="footer-wrap">
 +
            <div class="footer-contact">Contact Info.</div>
 +
            <p class="margin-bottom-10">WeChat Official Account:<i>谷饲嘉Grain Fed Plus</i></p>
 +
            <p>Email：<i>xxkk5905@gmail.com</i></p>
 +
        </section>
 +
    </footer>
 +
</body>
 +
<script>
 +
    let liTags = document.querySelectorAll(".top-nav-bar > ul > li");
 +
    let len = liTags.length;
 +
    for (let i = 0; i < len; i++) {
 +
        liTags[i].onclick = function (e) {
 +
            //先移除所有的点击样式
 +
            for (let j = 0; j < len; j++) {
 +
                liTags[j].classList.remove("active");
 +
            }
 +
            //再添加点击样式
 +
            let li = e.currentTarget;
 +
            li.classList.add("active");
 +
        }
 +
    }
 +
</script>
  
−
<div class="clear"></div>
+
</html>
−
 
+
−
 
+
−
 
+
−
<div class="column full_size">
+
−
 
+
−
<h1>Contribution </h1>
+
−
<H3>
+
−
Bronze Medal Criterion #4
+
−
</H3>
+
−
<p>Make a useful contribution for future iGEM teams. Use this page to document that contribution.
+
−
<br><br>
+
−
 
+
−
Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
+
−
 
+
−
<p>If you are making a contribution by adding information to an existing Part or creating a new Part, you must document your contribution on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a> for your team to be eligible for this criteria. You can use this page to link to that part and include additional information about your contribution.</p>
+
−
</P>
+
−
</DIV>
+

Latest revision as of 13:16, 19 October 2021

Shanghai_City_United

Contribution
BBa_K4093004
== Profile ==
Name: pMD19-T-xynA
Base Pairs: 3346bp
Origin: Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed
== Usage and Biology ==
Feed grains are whole grains such as corn, wheat and barley used in the feeding of livestock and poultry. At present, corn is the main fodder in the feed industry. Due to the increasing shortage of raw materials and the rising price, the development of the feed industry has been greatly limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain, and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat, cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP), which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore, we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed absorption efficiency.
Figure1. Demonstration of Partnership
== Construct design ==
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a expressing xylanase gene plasimd.
Figure 2. Schematic map of expression module of xynA .
BBa_K4093005
== Profile ==
Name: PUS-xynA
Base Pairs: 936bp
Origin: lactic acid bacteria, Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed
== Construct design ==
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a expressing xylanase gene module.
Figure 2. Schematic map of expression module of xynA and PUS.
BBa_K4093006
== Profile ==
Name: pSIP403-PUS-xyn A
Base Pairs: 8354bp
Origin: E. coli , Bacillus subtilis ,synthetic
Properties: Additive enzyme for baby poultry feed
== Construct design ==
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct a plasmid expressing xylanase gene, then we transformed them into Escherichia coli and Lactobacillus reuteri for further performance analysis.
Figure 2. Schematic map of expression system of xynA plasmids.
Contact Info.

WeChat Official Account:谷饲嘉Grain Fed Plus

Email：xxkk5905@gmail.com