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− | {{IGEM_TopBar}}
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− | {{Fujian_United}}
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| <html> | | <html> |
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− | <div class="column full_size"> | + | <link rel="stylesheet" type="text/css" |
| + | href="https://2021.igem.org/wiki/index.php?title=Template:Fujian_United/StyleCSS&action=raw&ctype=text/css"/> |
| | | |
− | <h1>Notebook</h1> | + | <div class="alpha-luco-content"> |
− | <p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p> | + | <!--navbar--> |
| + | <div class="navbar"> |
| + | <a class="logo" href="https://2021.igem.org/Team:Fujian_United"> |
| + | <img src="https://static.igem.org/mediawiki/2021/f/f3/T--Fujian_United--logo.jpeg" alt=""> |
| + | </a> |
| | | |
− | </div> | + | <!--nav--> |
− | <div class="clear"></div> | + | <ul class="list"> |
| + | <!--Team--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Team">Team</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Team">Team Members</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Attributions">Attributions</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Collaborations">Collaboration</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Partnership">Partnership</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
| + | <!--Modeling--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Model">Modeling</a> |
| + | </li> |
| | | |
| + | <!--Entrepreneurship--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Entrepreneurship">Entrepreneurship</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Entrepreneurship">Entrepreneurship</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Business_Plan">Business Plan</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
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− | <div class="column two_thirds_size"> | + | <!--Implementation--> |
− | <h3>What should this page have?</h3> | + | <li class="item"> |
− | <ul>
| + | <a href="https://2021.igem.org/Team:Fujian_United/Implementation">Implementation</a> |
− | <li>Chronological notes of what your team is doing.</li>
| + | </li> |
− | <li> Brief descriptions of daily important events.</li>
| + | |
− | <li>Pictures of your progress. </li>
| + | |
− | <li>Mention who participated in what task.</li>
| + | |
− | </ul>
| + | |
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− | </div> | + | <!--Human Practice--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Human Practice</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Integrated Human |
| + | Practice</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Communication">Communication</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Fundraising">Fundraising</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
− | <div class="column third_size"> | + | <!--Parts--> |
− | <div class="highlight decoration_A_full"> | + | <li class="item"> |
− | <h3>Inspiration</h3> | + | <a href="https://2021.igem.org/Team:Fujian_United/PartsCollection">Parts</a> |
− | <p>You can see what others teams have done to organize their notes:</p> | + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Parts_Collection">Parts |
| + | Collection</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Engineering">Engineering</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
− | <ul> | + | <!--Project--> |
− | <li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li> | + | <li class="item active"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Description">Project</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Description">Description</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Experiments">Experiments</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Results">Result</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/ProofOfConcept">Proof of Concept</a> |
| + | </li> |
| + | <li class="child-item active"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Notebook">Notebook</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United/Safety">Safety</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
− | <li><a href="https://2019.igem.org/Team:Georgia_State/Notebook">2019 Georgia State</a></li> | + | <!--Home--> |
− | <li><a href="https://2019.igem.org/Team:Newcastle/Notebook">2019 Newcastle</a></li> | + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Fujian_United">Home</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| | | |
| + | <!--图片--> |
| + | <div class="page-header"> |
| + | <img src="https://static.igem.org/mediawiki/2021/9/94/T--Fujian_United--bg_1.png" alt=""> |
| + | </div> |
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− | <li><a href="https://2020.igem.org/Team:IISER-Pune-India/Notebook">2020 IISER Pune India</a></li> | + | <!--内容--> |
− | <li><a href="https://2020.igem.org/Team:Lund/Notebook">2020 Lund</a></li> | + | <div class="content-middle-outer"> |
− | <li><a href="https://2020.igem.org/Team:NOVA_LxPortugal/Notebook">2020 NOVA LxPortugal</a></li> | + | <div class="content-middle"> |
− | <li><a href="https://2020.igem.org/Team:RDFZ-China/NoteBook">2020 RDFZ China</a></li> | + | <section class="article p-t-30 p-b-54"> |
− | </ul> | + | <h1 class="content-header">Notebook</h1> |
| + | |
| + | <table> |
| + | <tr> |
| + | <td colspan="2" |
| + | style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px"> |
| + | July |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td style="width: 20%">4<sup>th</sup></td> |
| + | <td>Prepare culture mediums.</td> |
| + | </tr> |
| + | <tr> |
| + | <td>5<sup>th</sup></td> |
| + | <td>Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.</td> |
| + | </tr> |
| + | <tr> |
| + | <td>6<sup>th</sup></td> |
| + | <td>Construction of the 4 plasmids.</td> |
| + | </tr> |
| + | <tr> |
| + | <td>7<sup>th</sup></td> |
| + | <td>Colony PCR, gel electrophoresis, and select single clones.</td> |
| + | </tr> |
| + | <tr> |
| + | <td>8<sup>th</sup></td> |
| + | <td>Plasmids extraction from E. Coli, and sent to the DNA sequencing company.</td> |
| + | </tr> |
| + | <tr> |
| + | <td>9<sup>th</sup></td> |
| + | <td>Verify the DNA sequencing resuls and carry out the transformation of plasmids into the |
| + | Saccharomyces cerevisiae by Heat-Shock approach. |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td>11<sup>st</sup></td> |
| + | <td>Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in |
| + | 1ml culture mediums. |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td>12<sup>nd</sup></td> |
| + | <td>Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate |
| + | efficient growth. Hence, we were not able to carry out the enzyme acitivety tests. |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td>13<sup>th</sup></td> |
| + | <td>Carry out fermentation performance test by testing the concentrations of glucose and ethanol. |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td colspan="2" |
| + | style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px"> |
| + | August |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td colspan="2">Repeat the plasmids construction experiments and the yeast culture by AQ yeast.</td> |
| + | </tr> |
| + | <tr> |
| + | <td colspan="2" |
| + | style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px"> |
| + | September |
| + | </td> |
| + | </tr> |
| + | <tr> |
| + | <td colspan="2">Carry out enzyme activity tests and fermentation performance tests.</td> |
| + | </tr> |
| + | </table> |
| + | </section> |
| + | </div> |
| + | </div> |
| + | |
| + | <!--Footer--> |
| + | <div class="footer"> |
| + | <div class="content-middle"> |
| + | <hr> |
| + | <span>WeChat Official Account:</span> |
| + | <span class="red">iGEM Alpha Luco</span> |
| + | </div> |
| + | </div> |
| </div> | | </div> |
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| </html> | | </html> |